P02774 Vitamin D-binding protein precursor 52.95 5.4 Cell communication c, a,b S,C P08833 Insulin-like growth factor binding protein 1 precursor 27.88 5.1 Cell communication c, a P02760 AMBP protein precursor [Contains: Alpha-1-microglo 38.98 6 Cell communication c, a,b S P02751 Fibronectin precursor 262.58 5.5 Cell communication d S P00450 Ceruloplasmin precursor 122.19 5.4 Cell communication c S,C P02763 Alpha-1-acid glycoprotein 1 precursor 23.49 4.9 Cell communication a S,C P02452 Collagen alpha 1 (I) chain precursor 138.87 5.7 Cell communication d S P02790 Hemopexin precursor 51.66 6.6 Defense response c S,C P04217 Alpha-1B-glycoprotein precursor 54.25 5.6 Defense response c, a S,C P01042 Kininogen precursor 71.93 6.3 Enzyme activity c S,C P02749 Beta-2-glycoprotein I precursor 38.28 8.3 Cell communication S,C P61769 Beta-2-microglobulin precursor 13.7 6.1 Signal transduction c, a P02753 Plasma retinol-binding protein precursor 22.99 5.8 Cell communication S P09486 SPARC precursor 34.61 4.7 Development d S P19652 Alpha-1-acid glycoprotein 2 precursor 23.59 5 Cell communication S P00747 Plasminogen precursor 90.55 7 Enzyme activity S,C P02771 Alpha-fetoprotein precursor 68.66 5.5 Cell communication b S P01236 Prolactin precursor 25.86 6.5 Signal transduction P02461 Collagen alpha 1 (III) chain precursor 138.54 6.2 Cell communication P02647 Apolipoprotein A-I precursor 30.76 5.6 Cell communication d S,C P80370 Delta-like protein precursor 41.12 5.4 Development P25311 Zinc-alpha-2-glycoprotein precursor 33.85 5.6 Cell proliferation P20908 Collagen alpha 1 (V) chain precursor 183.6 4.9 Cell communication P02765 Alpha-2-HS-glycoprotein precursor 39.31 5.4 Metabolism a,d S,C P13727 Eosinophil granule major basic protein precursor 25.21 6.2 Defense response P04196 Histidine-rich glycoprotein precursor 59.56 7.1 Enzyme activity c S Q16610 Extracellular matrix protein 1 precursor 60.66 6.3 Signal transduction S,C P01033 Metalloproteinase inhibitor 1 precursor 23.15 8.5 Metabolism S P43652 Afamin precursor 69.05 5.6 Cell communication S P08603 Complement factor H precursor 139.11 6.2 Defense response c S,C P12111 Collagen alpha 3 (VI) chain precursor 343.53 6.4 Cell communication c P41222 Prostaglandin-H2 D-isomerase precursor 21.01 7.7 Cell communication S P23142 Fibulin-1 precursor 77.24 5.1 Cell communication c S P17936 Insulin-like growth factor binding protein 3 precursor 31.64 9 Development
P01034 Cystatin C precursor 15.78 9 Enzyme activity P18065 Insulin-like growth factor binding protein 2 precursor 35.12 7.5 Cell communication S Q16270 Insulin-like growth factor binding protein 7 precursor 29.11 8.3 Cell communication d Q12841 Follistatin-related protein 1 precursor 34.97 5.4 Cell proliferation c P01008 Antithrombin-III precursor 52.59 6.3 Enzyme Activity c S,C P98160 Basement membrane-specific heparan sulfate proteo468.79 6.1 Cell communication c P10451 Osteopontin precursor 35.4 4.4 Cell communication c P09466 Glycodelin precursor 20.61 5.4 Development P12109 Collagen alpha 1 (VI) chain precursor 108.53 5.3 Cell communication d P51884 Lumican precursor 38.41 6.2 Cell communication c S Q14766 Latent transforming growth factor beta binding protein173.2 5.3 Enzyme activity P22692 Insulin-like growth factor binding protein 4 precursor 27.92 6.8 Metabolism P62328 Thymosin beta-4 4.9 5 Metabolism C Q12805 EGF-containing fibulin-like extracellular matrix protein54.62 5 Defense response S P10909 Clusterin precursor 52.48 5.9 Signal transduction c S P04004 Vitronectin precursor 54.29 5.6 Cell communication c S,C Q15582 Transforming growth factor-beta induced protein IG-H74.66 7.6 Cell proliferation d S P61626 Lysozyme C precursor 16.52 9.4 Enzyme activity C P00751 Complement factor B precursor 85.52 6.7 Metabolism c, a S P13987 CD59 glycoprotein precursor 14.16 6 Defense response c C P35555 Fibrillin-1 precursor 312.28 4.8 Development P08294 Extracellular superoxide dismutase [Cu-Zn] precurso 25.86 6.1 Metabolism Q9HC84 Mucin-5B precursor 590.47 6.2 Cell communication C P07998 Ribonuclease pancreatic precursor 17.63 9.1 Enzyme activity P02788 Lactotransferrin precursor 78.32 8.5 Defense response a S,C Q9UBC9 Small proline-rich protein 3 18.14 8.9 FNA C P01344 Insulin-like growth factor II precursor 20.12 9.5 Signal transduction Q14118 Dystroglycan precursor 97.56 8.7 Signal transduction P00748 Coagulation factor XII precursor 67.8 8 Enzyme activity S P07602 Proactivator polypeptide precursor [Contains: Saposi58.09 5.1 Metabolism P01019 Angiotensinogen precursor 53.14 5.9 Metabolism c S P01833 Polymeric-immunoglobulin receptor precursor 83.3 5.6 Cell communication c C P02766 Transthyretin precursor 15.87 5.5 Cell communication c, a S P02768 Serum albumin precursor 69.35 5.9 Cell communication b,d S,C
P05156 Complement factor I precursor 65.7 7.7 Defense response S Q15113 Procollagen C-proteinase enhancer protein precurso 47.95 7.4 Development S P11464 Pregnancy-specific beta-1-glycoprotein 1 precursor 47.21 8.3 FNA c P29279 Connective tissue growth factor precursor 38.05 8.4 Metabolism Q9UGM5 Fetuin-B precursor 42.08 5 Metabolism P02671 Fibrinogen alpha/alpha-e chain precursor 94.96 5.7 Cell proliferation S,C P00746 Complement factor D precursor 26.99 8.1 Defense response S P02748 Complement component C9 precursor 63.16 5.4 Defense response c S P07476 Involucrin 68.45 4.6 Development C P05090 Apolipoprotein D precursor 21.26 5.1 Metabolism c S P01243 Chorionic somatomammotropin hormone precursor 25 5.3 Signal transduction a P08123 Collagen alpha 2(I) chain precursor 129.44 9 Cell communication d P05362 Intercellular adhesion molecule-1 precursor 57.81 8.3 Cell communication P24593 Insulin-like growth factor binding protein 5 precursor 30.55 8.6 Cell communication P02679 Fibrinogen gamma chain precursor 51.5 5.4 Cell proliferation c S,C Q07654 Trefoil factor 3 precursor 8.62 5.7 Defense response P35321 Cornifin A 9.86 8.9 Development C O95633 Follistatin-related protein 3 precursor 27.64 6.4 FNA P31949 Calgizzarin 11.72 6.6 Metabolism P61916 Epididymal secretory protein E1 precursor 16.55 7.6 Metabolism O43278 Kunitz-type protease inhibitor 1 precursor 58.38 5.9 Enzyme activity P04278 Sex hormone-binding globulin precursor 43.76 6.2 Cell communication S P16070 CD44 antigen precursor 81.54 5.1 Cell communication Q13421 Mesothelin precursor 69.03 6 Cell communication c P01028 Complement C4 precursor 192.75 6.7 Defense response c S,C P08174 Complement decay-accelerating factor precursor 41.38 7.8 Defense response P31025 Von Ebner's gland protein precursor 19.23 5.4 Defense response c, a Q10588 ADP-ribosyl cyclase 2 precursor 35.72 8 Defense response S P03973 Antileukoproteinase 1 precursor 14.31 9.1 Enzyme activity C P04080 Cystatin B 11.12 7 Enzyme activity a C P02652 Apolipoprotein A-II precursor 11.16 6.3 Metabolism S P10599 Thioredoxin 11.59 4.8 Metabolism C Q96IY4 Carboxypeptidase B2 precursor 48.39 7.6 Metabolism S P11684 Uteroglobin precursor 9.98 5 Signal transduction
P55290 Cadherin-13 precursor 78.27 4.8 Cell organization P22105 Tenascin-X precursor 464.42 5.2 Cell communication S P80188 Neutrophil gelatinase-associated lipocalin precursor 22.57 9 Cell communication S,C P01024 Complement C3 precursor 187.15 6 Defense response c S,C P01860 Ig gamma-3 chain C region 32.31 7.9 Defense response S P48745 NOV protein homolog precursor 39.14 8.1 Development P03952 Plasma kallikrein precursor 71.35 8.6 Enzyme activity S P17900 Ganglioside GM2 activator precursor 20.8 5.2 Enzyme activity P04156 Major prion protein precursor 27.64 9.1 Metabolism P07988 Pulmonary surfactant-associated protein B precursor42.1 5.3 Metabolism c P01215 Glycoprotein hormones alpha chain precursor 13.06 8.5 Signal transduction Q14767 Latent transforming growth factor-beta-binding protei 195.04 5.1 Signal transduction P00734 Prothrombin precursor 70.02 5.6 Enzyme activity c S P02787 Serotransferrin precursor 77.03 6.8 Defense response a,c,d S,C P09228 Cystatin SA precursor 16.43 4.8 Enzyme activity Q03591 Complement factor H-related protein 1 precursor 37.64 7.7 Cell communication S P12830 Epithelial-cadherin 97.4 4.6 Cell communication P14543 Nidogen-1 136.37 5.1 Cell communication P16949 Stathmin 17.16 5.8 Cell communication P49747 Cartilage oligomeric matrix protein 82.78 4.3 Cell communication S P62258 14-3-3 protein epsilon 29.16 4.6 Cell communication d P63104 14-3-3 protein zeta/delta 27.73 4.7 Cell communication d C P68871 Hemoglobin subunit beta 15.86 6.8 Cell communication a S Q13908 Alpha-2 type V collagen 5.22 4.7 Cell communication Q14036 Alpha-2 type I collagen 3.74 8.6 Cell communication Q14112 Nidogen-2 151.3 5.1 Cell communication Q5XKQ4 Alpha-2-glycoprotein 1, zinc 34.22 5.7 Cell communication S Q6GRB6 Fetuin-B 42.03 6.5 Cell communication S Q6GTW4 Cadherin 13, preproprotein 78.27 4.8 Cell communication Insulin-like growth factor binding protein, acid labile Q8TAY0 subunit 66.03 6.3 Cell communication S Q92496 Complement factor H-related protein 4 37.3 5.2 Cell communication P35609 Alpha-actinin-2 103.79 5.3 Cell organization P53485 Actin, cytoplasmic 2 41.74 5.3 Cell organization
P68133 Actin, alpha skeletal muscle 42.02 5.2 Cell organization S P02675 Fibrinogen beta chain 55.89 8.5 Cell proliferation d, a S,C P60709 Actin, cytoplasmic 1 41.71 5.3 Cell proliferation S,C P61981 14-3-3 protein gamma 28.15 4.8 Cell proliferation d P01011 Alpha-1-antichymotrypsin 47.62 5.3 Defense response S,C P01595 Ig kappa chain V-I region Bi 12.02 5.2 Defense response P01598 Ig kappa chain V-I region EU 11.78 8.6 Defense response S P01603 Ig kappa chain V-I region Ka 11.89 9 Defense response P01605 Ig kappa chain V-I region Lay 11.83 8 Defense response P01621 Ig kappa chain V-III region NG9 10.72 6.3 Defense response S P01622 Ig kappa chain V-III region Ti 11.78 8.7 Defense response S P01624 Ig kappa chain V-III region POM 11.91 9.1 Defense response S P01625 Ig kappa chain V-IV region Len 12.63 7.9 Defense response S P01766 Ig heavy chain V-III region BRO 13.22 6.4 Defense response P01767 Ig heavy chain V-III region BUT 12.37 9.3 Defense response P01857 Ig gamma-1 chain C region 36.08 8.5 Defense response S,C P01859 Ig gamma-2 chain C region 35.86 7.7 Defense response S,C P01861 Ig gamma-4 chain C region 35.92 7.2 Defense response S,C P01876 Ig alpha-1 chain C region 37.63 6.1 Defense response S,C P01877 Ig alpha-2 chain C region 36.49 5.7 Defense response S,C P04083 Annexin A1 38.56 6.6 Defense response d S,C P04206 Ig kappa chain V-III region GOL 11.82 9.3 Defense response S P04434 Ig kappa chain V-III region VH 12.75 5.6 Defense response S P05109 Protein S100-A8 10.83 6.5 Defense response S,C P05155 Plasma protease C1 inhibitor 55.12 6.1 Defense response c S P05164 Myeloperoxidase* 83.81 9.2 Defense response C P06681 Complement C2 83.21 7.2 Defense response S P06702 Protein S100-A9 13.23 5.7 Defense response S,C P08571 Monocyte differentiation antigen CD14 40.05 5.8 Defense response S P08697 Alpha-2-antiplasmin 54.53 5.9 Defense response c S P10643 Complement component C7 93.46 6.1 Defense response S P15814 Immunoglobulin lambda-like polypeptide 1 22.95 10.1 Defense response P18135 Ig kappa chain V-III region HAH 14.06 7.8 Defense response S P20160 Azurocidin* 26.87 9.8 Defense response
P40121 Macrophage capping protein 38.49 5.9 Defense response d P49913 Antibacterial protein FALL-39* 19.29 9.5 Defense response Long palate, lung and nasal epithelium carcinomaassociated protein 1* 52.41 6.7 Defense response C Q8TDL5 Q9UGM3 Glycoprotein 340* 260.57 5.2 Defense response P05452 Tetranectin 22.55 5.5 Development S P06741 Myosin light chain 3, skeletal muscle isoform 16.54 4.6 Development P07355 Annexin A2 38.45 7.6 Development d S,C P07996 Thrombospondin-1 129.33 4.7 Development S P36955 Pigment epithelium-derived factor 46.31 6 Development c S Q9UBI9 Headcase protein homolog 58.8 8.9 Development P30740 Leukocyte elastase inhibitor* 42.71 5.9 Enzyme activity C P05543 Thyroxine-binding globulin 46.29 5.9 Enzyme activity c S P05546 Heparin cofactor 2 57.03 6.4 Enzyme activity S P08185 Corticosteroid-binding globulin 45.11 5.6 Enzyme activity c S P09525 Annexin A4 35.73 5.9 Enzyme activity d Q02325 Plasminogen-related protein B 10.96 6.1 Enzyme activity S P02750 Leucine-rich alpha-2-glycoprotein 38.15 6.5 FNA S P13796 Plastin-2* 70.24 5.2 FNA S,C Q2XP30 Mutant beta-globin 2.4 5.5 FNA Q5SYV9 Lipocalin 2 22.77 8.7 FNA Q6EMK4 Vasorin 71.67 7.2 FNA Q92520 Protein FAM3C 22.15 7.8 FNA Q9UJ14 Gamma-glutamyltransferase 4 70.79 6.4 FNA Q9Y5Z4 Heme-binding protein 2 22.86 4.6 FNA O00187 Mannan-binding lectin serine protease 2 75.64 5.4 Metabolism S P00918 Carbonic anhydrase 2 29.1 6.9 Metabolism P01023 Alpha-2-macroglobulin 163.17 6 Metabolism S P06727 Apolipoprotein A-IV 45.37 5.3 Metabolism c,d S P08709 Coagulation factor VII 51.56 6.9 Metabolism P15144 Aminopeptidase N 109.31 5.3 Metabolism S P18669 Phosphoglycerate mutase 1 28.65 6.8 Metabolism d C P19440 Gamma-glutamyltranspeptidase 1 61.37 6.7 Metabolism P22352 Glutathione peroxidase 3 25.49 8.2 Metabolism
P36222 Chitinase-3-like protein 1 42.6 8.7 Metabolism P43251 Biotinidase 58.88 5.5 Metabolism S P55058 Phospholipid transfer protein 54.7 6.5 Metabolism S P60174 Triosephosphate isomerase 26.52 6.5 Metabolism d C P62979 40S ribosomal protein S27a 9.41 9.9 Metabolism P62987 60S ribosomal protein L40 6.18 10.3 Metabolism P62988 Ubiquitin 8.56 6.6 Metabolism C Q16819 Meprin A subunit alpha 84.31 5.4 Metabolism S Q53XZ5 Tyrosine 3-monooxygenase/tryptophan 5- monooxygenase 29.16 4.6 Metabolism Q5T986 Inter-alpha (Globulin) inhibitor, H2 polypeptide 106.4 6.4 Metabolism CDNA FLJ32377 fis, clone SKMUS1000014, highly similar to Polyubiquitin 9 43.62 6 Metabolism Q96MH4 Q96PQ8 Factor VII active site mutant immunoconjugate 75.5 6.6 Metabolism Q14406 Chorionic somatomammotropin hormone-like 1 22.63 5.6 Signal transduction Q53YY1 Angiotensinogen 53.09 5.9 Signal transduction S Q7Z7P3 Coagulation factor II, 69.96 5.6 Signal transduction Q86VW7 Colony stimulating factor 1 receptor, 108 6 Signal transduction Q8WZA4 Collectin placenta 1 81.47 5.5 Signal transduction Proteins that were identified in experiment are listed with their SwissProt or TrEMBL accession 1. Molecular weight (kda) 2 and pi 3 calculated using CalPI/ tool available on SwissProt website. 4 Functional annotation is performed using procedure outlined in methods section. FNA-Function not assigned. 5 AF proteins that were also found in other amniotic fluid (a I,b II,c III ) and amnion cell (d IV ) studies are marked accordingly. 6 AF proteins that are also present in Serum (S) and Cervico-vagianl Fluid (C) are also marked (see results section for details). * Proteins identified in amniotic fluid with infection. I - Park, SJ et. al. Proteomics, 2006, 6, (1), 349-63; II - Nilsson S et. al. J. Proteome Research, 2004, 3, (4), 884-889; III - Michel, P. E. et. al. Electrophoresis, 2006, 7, 7.; IV- Tsangaris, G. et. al. Electrophoresis, 2005, 26, (6), 1168-73; Proteins are also color coded for their role in fetal biology (see results for details): Red-Complement and Coagulation cascades, Grey-Metabolism, Green - Defense response, Yellow-Signal transduction, Orange-Development, and Cyan- Transport