Supplement to "Basic Research in Cardiology", Vol. 80 (1985) Editors: R. Jacob (fiibingen) and W. Schaper (Bad Nauheim) Adult heart muscle cells Isolation, properties and applications Edited by H. M. Piper and P. G. Spieckermann With contributions by J. C. Altona, 1. A. Bailey, M. Banfalvi, M. Bechem, Z. Bendukidze, A. Beresewicz, B. Bertier-Savalle, C. H. F. Bloys van Treslong, J. V. Bonventre, T. K. Borg, M. Borgers, G. Brum, E. Carmeliet, J. Y. Cheung, J. Eckel, S. A. E. Finch, W. Graf, D. R. Harris, J. F. Hutter, G. Isenberg, S. L. Jacobson, H. Kammermeier, U. Klockner, R. Koch, B. Koidl, W. Kubler, A. Leaf, E. Lundgren, B. Maisch, F. Marotte, D. Mascher, A. A. Noronha-Dutra, W. Osterrieder, F. Pieper, H. M. Piper, L. Pott, T. Powell, 1. Probst, L. Rappaport, B. Rauch, H. Reinauer, J. L. Samuel, R. Spahr, P. G. Spieckermann, K. Schwartz, P. Schwartz, T. A. Schwarzfeld, E. M. Steen, A. Stier, L. Terracio, F. Thone, H. A. Tritthart, G. Trube, V. von Tscharner, F. Valenzuela, G. Van der Heyden, A. Van der Laarse, J. Veerecke, L. Ver Donck, B. Wein, N. Woolf, G. Zernig Springer-Verlag Berlin Heidelberg GmbH
ISBN 978-3-662-1 1043-0 ISBN 978-3-662-11041-6 (ebook) DOI 10.1007/978-3-662-11041-6 This work is subject to copyright. AII rights are reserved, whether the whole or part of the material is concerned, specifically those oftranslation, reprinting, re-use ofillustrations, broadcasting, reproduction by photocopying machine or similar means, and storage in data banks. Under 54 ofthe German Copyright law, where copies are made for other than private use, a fee is oavable to the publisher, the amount ofthe fee to be determined by agreement with the publisher. 1984 by Springer-Verlag Berlin Heidelberg Originally published by Dr. Dietrich SteinkopffVerlag GmbH & Co. KG. Darmstadt in 1984 Softcover reprint ofthe hardcover Ist edition 1984 The use of registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use.
Foreword With this volume results become generally available of a recent conference that was the first to discuss properties of isolated heart muscle cells from the adult myocardium as a new cardiological research tool. The conference was held as an International Erwin Riesch Symposium under the auspices of the Deutsche Gesellschaft fur Herz- und Kreislaufforschung at Spangenberg Castle, March 22-25, 1984. It was made possible through funding by the Erwin Riesch Foundation and the Bayer AG which deserve our gratitude for their generosity. It may not be fully reflected in this collection of papers that the atmosphere of this meeting was unique. The reason for this resided apparently in the still rudimentary experiences with the adult cardiocyte model that is now widely acknowledged to be very promising. Thus, quite basic interests brought together experts from different biological disciplines who in respect to this new model were all still "beginners". It was noteworthy that such common interests exerted a unifying effect upon otherwise rather separated fields of science. The main task of the years to come will certainly consist in improving isolation and culturing conditions for adult myocytes in order to fully establish the isolated cell model in heart research. Very basic investigations are still to be done to achieve this goal. For this, cooperation and information exchange is urgently needed among the yet limited number of groups working actively with isolated adult myocytes. The broad perspectives of a further development of this model are elucidated not only by the topics of the articles in this volume, but also by the summary of the informal discussions at the conference. We hope that these proceedings may contribute to the progress in research with cellular models of the myocardium. H. M. Piper P. G. Spieckermann
Contents Foreword Isolated adult heart cells. The development of a new experimental model T. Powell........ Ca-tolerant guinea-pig ventricular myocytes as isolated by pronase in the presence of 250 11M free calcium Z. Bendukidze, G. Isenberg, U. KI6ckner.......................... 13 Guinea-pig atrial cardioballs M. Bechem, F. Pieper, L. Pott Determination of isolated myocyte viability: Staining methods and functional criteria J. y. Cheung, A. Leaf, 1. V. Bonventre............ 23 Sarcolemma-bound calcium. Its importance for cell viability M. Borgers, F. Thone, L. Ver Donck..... 31 Anoxic injury of adult cardiac myocytes H. M. Piper, P. Schwartz, R. Spahr, J. F. Hutter, P. G. Spieckermann 37 Non-invasive measurements of cell surface receptors I. A. Bailey, V. von Tscharner, D. R. Harris 43 Surface antigens of adult heart cells and their use in diagnosis B. Maisch........ 47 Substrate utilization of adult cardiac myocytes R. Spahr, I. Probst, H. M. Piper....... 53 Characteristics of lactate transfer in isolated cardiac myocytes H. Kammermeier, B. Wein, W. Graf.... 57 The insulin receptor of adult heart muscle cells J. Eckel, H. Reinauer......... 61 Kinetics of cellular uptake of tracers used in myocardial scintigraphy B. Rauch, W. Kubler.... 65 Adhesion of cardiac myocytes to extracellular matrix components E. Lundgren, L. Terracio, T. K. Borg..... 69 Development of new intercellular contacts between adult cardiac myocytes in culture P. Schwartz, H. M. Piper, R. Spahr, J. F. Hutter, P. G. Spieckermann....... 75 Long-term primary cultures of adult human and rat cardiomyocytes S. L. Jacobson, M. Banfalvi, T. A. Schwarzfeld........ 79 Morphological dedifferentiation of adult cardiac myocytes in coculture with hepatocytes R. Spahr, H. M. Piper, P. Schwartz, I. Probst, P. G. Spieckermann.......... 83 Electrophysiological properties of isolated ventricular myocytes T. Powell.... 87 5 8 19
The effect of cyanide on the K-current in guinea-pig ventricular myocytes G. Van der Heyden, 1. Vereecke, E. Carme1iet.............. Measurements of single-channel currents in the membrane of isolated cells: A TP-dependence of K + -channels G. Trube................................. 97 Evidence for Ca-mediated inactivation of lea in dialysed guinea-pig atrial cardioballs M. Bechem, L. Pott........................... 101 93 Cardiac membrane currents and energetic state W. Osterrieder, G. Brum....... Simultaneous measurements of action potentials and contractions in single cultured adult and embryonic heart muscle cells B. Koidl, G. Zernig, H. A. Tritthart The two components in the shortening of unloaded ventricular myocytes: Their voltage dependence G. Isenberg, A. Beresewicz, D. M ascher, F. Valenzuela........ 107 III 117 Temperature dependence of verapamil action H. M. Piper, R. Koch, P. G. Spieckermann Microtubules and desmin filaments during the onset of heart growth in the rat L. Rappaport, 1. L. Samuel, B. Bertier-Savalle, F. Marotte, K. Schwartz The correlation between catecholamine and lipid peroxidation induced damage in heart cells A. A. Noronha-Dutra, E. M. Steen, N. Woolf.......... 133 123 129 Anoxia in neonatal rat heart cell cultures A. Van der Laarse, 1. C. Altona, C. H. F. Bloys van Treslong 137 Enzyme release and glycolytic energy production H. M. Piper, R. Spahr, 1. F. Hutter, P. G. Spieckermann 143 Anoxia influences the lateral diffusion of a lipid probe in the plasma membrane of isolated cardiac myocytes S. A. E. Finch, H. M. Piper, P. G. Spieckermann, A. Stier............... 149 Summaries of the discussions of the symposium 1. Y. Cheung, B. Koidl, H. M. Piper, G. Trube 153 Abstracts of the poster-session on the adult myocyte model 160 Author Index 165 Subject Index 166