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INTERNATIONAL STANDARD ISO 4716 Third edition 2013-08-01 Essential oil of vetiver [Chrysopogon zizanioides (L.) Roberty, syn. Vetiveria zizanioides (L.) Nash] Huile essentielle de vétiver [Chrysopogon zizanioides (L.) Roberty, syn. Vetiveria zizanioides (L.) Nash] Reference number ISO 4716:2013(E) ISO 2013

ISO 4716:2013(E) COPYRIGHT PROTECTED DOCUMENT ISO 2013 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO s member body in the country of the requester. ISO copyright office Case postale 56 CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyright@iso.org Web www.iso.org Published in Switzerland ii ISO 2013 All rights reserved

ISO 4716:2013(E) Contents Page Foreword...iv 1 Scope... 1 2 Normative references... 1 3 Terms and definitions... 1 4 Requirements... 1 4.1 Appearance... 1 4.2 Colour... 1 4.3 Odour... 2 4.4 20 Relative density at 20 C, d 20... 2 4.5 Refractive index at 20 C... 2 4.6 Optical rotation at 20 C... 2 4.7 Miscibility in ethanol 80 % volume fraction at 20 C... 2 4.8 Acid value... 2 4.9 Chromatographic profile... 2 4.10 Flashpoint... 3 5 Sampling... 3 6 Test methods... 3 6.1 20 Relative density at 20 C, d 20... 3 6.2 Refractive index at 20 C... 3 6.3 Optical rotation at 20 C... 3 6.4 Miscibility in ethanol 80 % volume fraction at 20 C... 3 6.5 Acid value... 3 6.6 Chromatographic profile... 3 7 Packaging, labelling, marking and storage... 4 Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of vetiver [Chrysopogon zizanioides (L.) Roberty, syn. Vetiveria zizanioides (L.) Nash]... 5 Annex B (informative) Flashpoint...10 Bibliography...11 ISO 2013 All rights reserved iii

ISO 4716:2013(E) Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2, www.iso.org/directives. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received, www.iso.org/patents. Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement. The committee responsible for this document is ISO/TC 54, Essential oils. This third edition cancels and replaces the second edition (ISO 4716:2002), which has been technically revised. iv ISO 2013 All rights reserved

INTERNATIONAL STANDARD ISO 4716:2013(E) Essential oil of vetiver [Chrysopogon zizanioides (L.) Roberty, syn. Vetiveria zizanioides (L.) Nash] 1 Scope This International Standard specifies certain characteristics of the essential oil of vetiver [Chrysopogon zizanioides (L.) Roberty, syn. Vetiveria zizanioides (L.) Nash], growing in Reunion Island, China, Haiti, Indonesia, Madagascar, Brazil, and Comoros Island in order to facilitate assessment of its quality. 2 Normative references The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 210, Essential oils General rules for packaging, conditioning and storage ISO/TR 211, Essential oils General rules for labelling and marking of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Determination of refractive index ISO 592, Essential oils Determination of optical rotation ISO 875, Essential oils Evaluation of miscibility in ethanol ISO 1242, Essential oils Determination of acid value ISO 11024 (all parts), Essential oils General guidance on chromatographic profiles 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 essential oil of vetiver essential oil obtained by steam distillation of the roots of Chrysopogon zizanioides (L.) Roberty, syn. Vetiveria zizanioides (L.) Nash of the Poaceae family Note 1 to entry: For information on the CAS number, see ISO/TR 21092. [2] 4 Requirements 4.1 Appearance Viscous liquid. 4.2 Colour From yellowish brown to reddish brown. ISO 2013 All rights reserved 1