Legionella pneumophila SG1 Kit for the CellStream Ultrafast Detection Including Viability Assessment Speed Separation, concentration and purification of Legionella in 1-2 hours Specificity High specificity and sensitivity Superior monoclonal antibody pools Detection of viable but non-culturable Legionella Easy handling Adaptable to any matrix Flexible integration into any workflow Compatible with flow cytometry, PCR, cultivation method, microscopy, etc.
rqmicro developed this highly specific kit to simplify and accelerate the detection of Legionella pneumophila SG1 in water samples. Together with the CellStream it opens a new chapter in the field of pathogen detection and in the monitoring of water quality. Kit Components Cartridges: Magnetic particles: Staining dye: Viability dye: Positive control: Filters: Buffer 1: Buffer 2: 24 cartridges (96 tests) Magnetic particles, conjugated to a monoclonal antibodies Labelling antibody; Ex/Em 493/518 nm Propidium iodide as an indicator of membrane integrity; Ex/Em 535/617 nm Lyophilised, chemically fixed, freeze-dried Legionella pneumophila SG1-cells (Philadelphia) 100 polycarbonate filters; pore size 0.22 µm; Ø 47 mm Washing buffer Separation buffer Flexible Integration into Your Workflow 1. Concentration 2. Labelling 3. Automatic IMS on Disposable Cartridge 4. Analysis Quantitative results within 1-2 hours following a straightforward workflow from filtration to quantification using standard flow cytometry (FCM). rqmicro AG Page 2
Rapid Sample Preparation for Flow Cytometric Analysis rqmicro s kits are suitable for all standard flow cytometers. Using FCM in combination with the CellStream, water laboratories gain new insights into water systems due to specific live and dead staining of pathogens and the possibility of monitoring processes in real time, e.g. disinfection. Example of a flow cytometric quantification of Legionella pneumophila SG1 cells after the immunomagnetic separation with the CellStream before (left) and after (right) a successful disinfection. The sample is stained with staining dye (green) and viability dye (red). In the dot plot on the left, Legionella cells are green. The cells are viable and possess intact membranes so the viability dye does not stain them red. On the dot plot on the right, the Legionella cells are red and green. The disinfection process destroyed the integrity of the membrane and the cells are no longer viable. Pick the Analytical Method of Your Choice In addition to flow cytometry, the CellStream supports other analytical methods (e.g. cultivation method, PCR, fluorescence microscopy). Contact our experts for more information. rqmicro AG Page 3
Performance Sample purity: 95-99.9 % Limit of detection: 20-50 cells / liter* Background noise: 0-25 events / liter* Separation time: 35 minutes *Dependent on flow cytometry instrumentation used Performance tests show a recovery of > 80 % and a broad working range. Triplicates per data point. The lower detection limit is at around 20-50 cells per liter. Equivalence to ISO Method The rqmicro testing procedure in combination with flow cytometry achieves > 98 % correlation with the current gold standard (ISO method). The sensitivity of the rqmicro method is 5-10 fold higher. Eight measurements per data point. Equivalent or more accurate results achieved in 1-2 hours instead of in 10 days. rqmicro AG Page 4
Specificity Cross reactivity experiments with the rqmicro Legionella pneumophila SG1 Kit using various SG1 strains portray high specificity and a complete coverage of SG1. Additional cross reactivity experiments are ongoing. For up-to-date information, please visit www.rqmicro.com. Organism L.p. SG1 Antibody binding Legionella pneumophila Philadelphia DSM 7513 + Legionella pneumophila OLDA DSM 25200 + Legionella pneumophila Knoxville DSM 25070 + Legionella pneumophila Bellingham DSM 25214 + Legionella pneumophila Benidorm DSM 25199 + Legionella pneumophila Oxford DSM 25213 + Legionella pneumophila Allentown/France ATCC 43106 + Legionella pneumophila Camperdown ATCC 43113 + Legionella pneumophila Heysham ATCC 43107 + No Cross Reactivity Extensive tests confirmed the specificity of the Legionella pneumophila SG1 Kit and evidenced no cross reactivity with the following, relevant organisms from the water and food industry. An up-to-date list is available on www.rqmicro.com. Legionella pneumophila SG 2-14 Legionella longbeachae Staphylococcus aureus Legionella anisa Legionella micdadei Staphylococcus epidermidis Legionella birminghamensis Legionella oakridgiensis Campylobacter jejuni Legionella bozemanii Legionella parisiensis Escherichia coli Legionella cincinnatiensis Legionella parisiensis Salmonella typhimurium Legionella dumoffii Legionella rubilucens Citrobacteri gillenii Legionella jamestowniensis Legionella taurinensis Listeria monocytogenes Legionella jordanis Pseudomonas aeruginosa Listeria welshimeri Legionella londoniensis Pseudomonas fluorescens Listeria fleishmanii rqmicro AG Page 5
90% Downtime Reduction Disinfection procedures guided by rapid tests: are targeted save costs and energy result in less downtime for affected infrastructures Rapid testing method provides immediate process feedback Enables operators to take corrective measures and/or stop process once complete Accurate Detection of VBNC Bacteria Real time monitoring of a disinfection process at 70 C Viable but non-culturable (VBNC) bacteria refers to bacteria that are in a state of very low metabolic activity. They do not divide, but are alive and have the ability to become culturable once resuscitated. rqmicro s testing procedure reveals that after a disinfection process viable Legionella cells are potentially still present after 60 minutes. The cultivation method does not detect VBNC bacteria. FCM Cultivation Method The presence of VBNC bacteria does therefore question the accuracy of the current gold standard (ISO method). VBNC rqmicro AG Page 6
Who Can Benefit from rqmicro s Rapid Legionella Test Cooling tower operators Environmental service laboratories Healthcare Institutions (hospitals, nursing homes, etc.) Industrial plants Public facilities (schools, universities, etc.) Water supply companies Public swimming baths, wellness and spa sector, etc. Current Test Kits in Development: Our R&D team is constantly working on expanding our offer. To check our current kits in development and our news please check out our website: www.rqmicro.com rqmicro AG Page 7
Contact us: rqmicro AG Brandstrasse 24 8952 Zürich - Schlieren Switzerland Tel: +41 44 512 51 51 Sales support: sales@rqmicro.com General information: info@rqmicro.com www.rqmicro.com Copyright rqmicro AG 2017 Test Kit SG1 Leaflet 201704