Waters Mass Spectrometry Systems

Waters Mass Spectrometry Systems Mark N Groudas VP Americas Field Operations 25 th, July 2017 2016 Waters Corporation 1

The Science of What s Possible 2016 Waters Corporation 2

Waters MS Systems Portfolio Mass Detection Quan HRMS Research ACQUITY SQD2 QDa Xevo TQ-XS TQ-S micro TQD Xevo G2-XS QTof G2-XS Tof Vion IMS-Q-Tof SYNAPT G2-Si HDMS G2-Si MS Imaging System Universal Source Architecture: ESI UniSpray ESCi APCI ASAP APGC APPI ionkey/ms nanoesi REIMS DART DESI LAESI - LDTD HPLC UHPLC UPLC UPC 2 APGC Empower UNIFI MassLynx Progenesis 2016 Waters Corporation 3

2016 Waters Corporation 4

Xevo TQ-XS 2016 Waters Corporation 5

Xevo TQ-XS Most sensitive MS we have ever produced with market-leading performance Ultimate sensitivity from StepWave XS Tool-free probes for improved user experience Easy method transfer/accessible sensitivity with XDR detector Wider compound coverage with UniSpray 2016 Waters Corporation 6

Maximum robustness, with no compromise in performance Innovative off-axis design Ions are transferred from the source region with maximum efficiency Potential contaminants are actively filtered out Critical hardware components stay clean for longer Results are more reproducible The lab stays productive 2016 Waters Corporation 7

Key improvements to optimise performance for challenging compounds RF voltage constant improving robustness while mass switching Segmented quadrupole second stage focussed ion beam Wider profile first stage, more space for ion cloud Horizontal plates provide more controlled extraction of ions from gas flow less energetic collisions 2016 Waters Corporation 8

Improved Customer Experience Tool-free ESI and APCI probes New design: Completely tool-free Based on QDa design Takes <2 min to change the probe assembly Removed customer-assembled union at the top of the probe Helpful features included after customer feedback Probe adaptor IP-protected technology, patents in process Probe assembly 2016 Waters Corporation 9

Improved Customer Experience Xtended Dynamic Range detector Long-life photomultiplier technology Accessible sensitivity data for peptide Val-Tyr-Val UPLC/MRM ESI+, 6 orders linear dynamic range Easy method transfer to a more sensitive instrument 2016 Waters Corporation 10

Universal Ion Source Architecture Ultimate flexibility from the widest choice of ionization techniques ESI, APCI, ESCI nanoflow ESI APPI, APCI DESI APGC ASAP ionkey/ms 2016 Waters Corporation 11

One source for all UniSpray What is UniSpray? A novel ion source option exclusive to Waters Wider compound coverage Greater ionization efficiency Comparable robustness and linearity to ESI 2016 Waters Corporation 12

Dynamic range, speed and accuracy Innovative geometry and ion detection system, delivering: Dynamic range Sensitivity Mass Resolution Speed of analysis...simultaneously 2016 Waters Corporation 16

Vion 2016 Waters Corporation 17

Vion calculates a unique CCS value for each ion but. What is CCS? Important differentiating Characteristic of an ion Chemical Structure (mass, size) 3-dimensional Conformation (shape) Precise Physicochemical Property of an ion 2016 Waters Corporation 18

Benefits of Routine Ion Mobility Clarity cleaner mass spectra Confidence valuable CCS information for every ion System Productivity with UNIFI analytical workflows 2016 Waters Corporation 19

Routine ion mobility Seamlessly from sample to answers Just press START Simply Acquire Get to the Answers 2016 Waters Corporation 20

Benefits of Routine Ion Mobility 617.3076 m/z 236.6 Å2 Verapamil Metabolite 441.2758 m/z 289.1923 m/z 518.2235 m/z 2016 Waters Corporation 21

Benefits of Routine Ion Mobility Clarity 617.3076 m/z 236.6 Å2 Verapamil Metabolite Unlimited Product ion HDMS E 518.2235 m/z 2016 Waters Corporation 22

Vion measured CCS(N 2 ) values <2% time after time Reserpine - 609.28 m/z : 252 Å 2-0.10 % RSD LueEnk 556.27 m/z : 230 Å 2-0.15 % RSD Terfenadine 472.32 m/z : 230 Å 2-0.12 % RSD Verapamil 455.29 m/z : 211 Å 2-0.17 % RSD Sulphadimethoxine 311.08 m/z : 168 Å 2-0.15 % RSD Caffine 195.08 m/z : 136 Å 2-0.22 % RSD Data from 400 injections over 33 hours 2016 Waters Corporation 23

Acetamiprid Azoxystrobin Bromuconazole II Carbetamide Carbofuran Chlorbromuron Chloridazon (Pyrazon) Clethodim I Clethodim I fgt Clethodim II Clethodim II fgt Cyromazine DEET Demeton-S-methyl-sulfone Dichlorvos Diethofencarb fgt Disulfoton-sulfone Diuron Ethoprophos Fenamiphos sulphone Fenoxycarb Fenoxycarb fgt Fenpropimorph Fenpyroximat Flutriafol Formetanate Fosthiazate Furathiocarb Heptenophos Hexazinone Indoxacarb Isoxaben Linuron Mesotrione Metalaxyl Methabenzthiazuron Metobromuron Metolachlor Metribuzin Mevinphos trans Nitenpyram Oxadiazon pethoxamid phenthoate Pirimicarb Primicarb-desmethyl fgt* Propamocarb propargite Prosulfocarb Pymetrozine Pyraclostrobin Pyridaben Pyridaben fgt 1 Pyridate Pyriproxifen Simazine Spinosad Spirodiclofen Spiroxamine II Tebuconazole tepraloxydim Thiacloprid Thiodicarb Thiophanate-methyl Triasulfuron Triflumuron Trinexapac-ethyl Triticonazole Vamidothion % CCS Difference WIV ISP Study: SYNAPT/Vion Comparison for Pesticides between two labs UK vs Belgium Confidence 5 Synapt WIV ISP/Vion IMS Q ToF Comparison April 2015 4 3 2 1 0 Pesticide -1-2 -3-4 -5 Axis Title 2016 Waters Corporation 24

UNIFI UNIFI 1.8 Streamlined Instrument Controls Seamless Workflows Integrated Ion Mobility Tools Sophisticated Reporting UNIFI: Taking Data to Knowledge 2016 Waters Corporation 25

Thank you and have a great Conference! 2016 Waters Corporation 27