QUICK POLAR PESTICIDES METHOD

QUICK POLAR PESTICIDES METHOD

Quick Polar Pesticides Method QuPPe-Method allows the analysis of residues of very polar, non-quechers-amenable, pesticides in foods of plant origin PO (fruits, vegetables, cereals and processed products) and animal origin AO (milk and eggs). The new kit designed for the application of the QuPPe-PO method includes: Isotopically labeled analogues as internal standards (ISTDs); Standard mixtures of polar pesticides. Quick Method for the Analysis of numerous Highly Polar Pesticides in Foods of Plant Origin via LC-MS/MS involving Simultaneous Extraction with (QuPPe-Method) - Version 8.1 (March 2015, Document History, see page 58) Authors: M. Anastassiades; D. I. Kolberg; E. Eichhorn; A. Benkenstein; S. Lukačević; D. Mack; C. Wildgrube; I. Sigalov; D. Dörk; A. Barth The new kit designed for the application of the QuPPe-AO method includes: Centrifuge tubes containing ODS (octadecylsilane) as sorbent; Isotopically labeled analogues as internal standards (ISTDs); Standard mixtures of polar pesticides. Quick Method for the Analysis of Residues of numerous Highly Polar Pesticides in Food Commodities involving Simultaneous Extraction with and Determination via LC-MS/MS (QuPPe-AO-Method) II. Food of Animal Origin - Version 2 (Jan 2016, Document History, see page 16) Authors: M. Anastassiades; D. Kolberg; A. Benkenstein; S. Zechmann; Ready to use Standards: safer handling and application Cost-effective: frequently delivering fresh batches More accurate quantification: isotopically labeled analogues Evaluation of container/content interaction phenomena

PROCEDURE PLANT ORIGIN PRODUCTS ANIMAL ORIGIN PRODUCTS Weigh a representative portion of the sample homogenate into a 50 ml centrifuge tube Fresh fruits, vegetables and juices: 10 g ± 0,1 g Dried fruits and dried mushrooms: 5 g ± 0,05 g or 13,5 g ± 0,1 g of the re-hydrated and homogenized sample (corresponding to 5 g dried sample) Cereals, dried pulses and honey: 5 g ± 0,05 g Depending on the nature of the matrix, add water to a total content of ca. 10 g (Table 19) Add 10 ml acidified methanol (CH 3 OH + 1% F.A.) and 50 μl of IS (IS-working solution I) containing isotopically labeled analogues of one or more of the analytes of interest Weigh a representative portion of the sample homogenate into a 50 ml centrifuge tube Fresh milk and fresh eggs: 10 g ± 0,05 g Add water to a total content of ca. 10 g. In the case of cow milk (88% H 2 O) add 1,2 g of water and in the case of chicken eggs (76% H 2 O) add 2,4 g of water Add 10 ml acidified methanol (CH 3 OH + 1% F.A.) and 50 μl of IS (IS-working solution I) containing isotopically labeled analogues of one or more of the analytes of interest Shake vigorously for 1 min by hand or for 5-20 min by a mechanical shaker Shake vigorously by a mechanical shaker (Fresh products: 5-15 min; Dried products: 15-30 min). Alternatively shake by hand for 1 min; in the case of dried products the shaking by hand is to be followed by a soaking period (15-30 min) and a subsequent second vigorous shaking by hand (1 min) For Paraquat and Diquat determination the shaking (1 min) is followed by a thermal treatment of 15 min at 80 C in a water bath. Then shaking again for 1 min and wait for the sample to cool down to room temperature Centrifuge for 5 min at >3000 g Transfer an aliquot (2 ml) into a 10 ml tube, containing 100 mg of ODS sorbent (Code: QPPAO01) and 2 ml of acetonitrile Centrifuge for 5 min at 4000 rpm Shake vigorously for 1 min by hand Filter an aliquot (3 ml) of the extract into a sealable storage vessel Use plastic flasks if pesticides (Paraquat, Diquat, Streptomycin, Glyphosate, etc.) that tend to interact with glass-surfaces are present Centrifuge for 5 min at >3000 g Filter an aliquot (ca. 3-4 ml) of the cleaned-up extract into a sealable storage vessel Transfer one or more aliquots (1 ml) of the filtered extract into vials for LC analysis Transfer one or more aliquots (1 ml) of the filtered extract into vials for LC analysis

Method: QuPPe-AO V14 g MgSO₄, 1 g NaCl, 1 g SCTD, 0,5 0,1 g ODS Packaging 50 Tubes (10 ml) Code QPPAO01 STANDARD MIX 1.1 CRM3G11L344 Ethephon HEPA 16672-87-0 22987-21-9 Glyphosate AMPA 1071-83-6 1066-51-9 Glufosinate MPPA 51276-47-2 15090-23-0 N-Acetyl Glufosinate 73634-73-8 STANDARD MIX 1.2 CRM3G11L345 Ethephon HEPA Glyphosate 16672-87-0 22987-21-9 1071-83-6 AMPA Glufosinate MPPA 1066-51-9 51276-47-2 15090-23-0 N-Acetyl Glufosinate Fosetyl-Aluminium N-Acetyl AMPA 73634-73-8 39148-24-8 57637-97-5 Phosphonic acid 13598-36-2 STANDARD MIX 1.3 CRM3G11L346 Ethephon HEPA Glyphosate AMPA 16672-87-0 22987-21-9 1071-83-6 1066-51-9 Glufosinate MPPA N-Acetyl Glufosinate Fosetyl-Aluminium 51276-47-2 15090-23-0 73634-73-8 39148-24-8 N-Acetyl AMPA Phosphonic acid Maleic hydrazide Perchlorate 57637-97-5 13598-36-2 123-33-1 14797-73-0 Chlorate Cyanuric acid Bialaphos 14866-68-3 108-80-5 35597-43-4 STANDARD MIX 1.4 CRM3G11L347 Phosphonic acid Perchlorate Chlorate 13598-36-2 14797-73-0 14866-68-3 CRM3G11L348 Fosetyl-Aluminium Maleic hydrazide Perchlorate 39148-24-8 123-33-1 14797-73-0 STANDARD MIX 3 CRM3G11L349 Amitrole 61-82-5 Daminozide ETU PTU 1596-84-5 96-45-7 2122-19-2 Trimethylsulfonium Cyromazine 676-84-6 66215-27-8

website/e-commerce www.labinstruments.org e-mail info@labinstruments.it STANDARD MIX 4.1 CRM3G11L350 Diquat Paraquat 2764-72-9 4685-14-7 Daminozide 1596-84-5 N,N-Dimethylhydrazine 57-14-7 Cyromazine Trimethylsulfonium Nereistoxin 66215-27-8 676-84-6 1631-58-9 Melamine Propamocarb 108-78-1 24579-73-5 STANDARD MIX 4.2 CRM3G11L351 Amitrole ETU 61-82-5 96-45-7 Daminozide PTU Cyromazine Trimethylsulfonium 1596-84-5 2122-19-2 66215-27-8 676-84-6 Nereistoxin Melamine Propamocarb 1631-58-9 108-78-1 24579-73-5 Morpholine Diethanolamine Triethanolamine 110-91-8 111-42-2 102-71-6 STANDARD MIX 5 CRM3G11L352 ETU PTU 96-45-7 2122-19-2 STANDARD MIX 6 CRM3G11L353 Streptomycin Kasugamycin 57-92-1 6980-18-3 STANDARD MIX 7 CRM3G11L354 Morpholine Diethanolamine Triethanolamine 110-91-8 111-42-2 102-71-6 STANDARD MIX 8 CRM3J11L355 Water 1,2,4-Triazole Triazole-alanine Triazole-acetic acid 288-88-0 86362-20-1 28711-29-7 Triazole-lactic acid 1,2,3-Triazole N.A. 288-36-8

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