Excel UHPLC Columns info@ace-hplc.com Advanced Chromatography Technologies Ltd www.ace-hplc.com Australia & New Zealand contact: infor@winlab.com.au or call + 0
Outline Introduction Key Product Points Examples of Isocratic and Gradient HPLC UHPLC Key Product Characteristics Fixtures and Fittings Australia & New Zealand contact: infor@winlab.com.au or call + 0
Key Excel Features Available as NEW high efficiency, ultra-inert mm silica particles and suitable for UHPLC at 000bar (000psi) Nine selectivities including the unique C8-AR and C8-PFP Highly reproducibility: column-to-column and batch-to-batch Ultra-robust phases: NEW low dispersion column hardware and NEW High Stability Column (HSC ) packing technology Engineered with lower back pressures compared to other <mm phases due to mm particle size and frit technology Fully scalable to mm, mm and 0mm (Usable at lower pressures giving a performance boost to HPLC) Australia & New Zealand contact: infor@winlab.com.au or call + 0
Using Excel C8-PFP Selectivity & Throughput (Isocratic) Aim: obtain R s. in shortest possible time for mixture Waters XBridge C8 0 x. mm.00 ml/min R s =. HPLC: µm C8 t r =.0 mins (L/d p = ) Waters ACQUITY. BEH C8 0 x. mm 0. ml/min R s =. UHPLC: <µm C8 t r =.9 mins (L/d p =.9) To maintain R s and reduce run time, keep L / d p ratio constant ~ x Quicker Excel C8-PFP 0 x. mm.0 ml/min UHPLC: Excel µm C8-PFP t r =0. mins R s =. R s =.9 Using UHPLC and selectivity, it is possible to dramatically improve resolution allowing shorter columns & increased flow rates Sample: ),,dimethoxybenzene, ),-dimethoxybenzene, ),,-trimethoxybenzene, ) toluene (reference). Mobile phase 0:0 MeOH / H O; Temperature 0 C All trademarks are recognised Australia & New Zealand contact: infor@winlab.com.au or call + 0
Using Excel C8-PFP Selectivity & Throughput (Gradient) Aim: obtain R s. in shortest possible time for mixture C8 00 x. mm ml/min, t G = 9 min max pressure: 9 bar 0 min cycle time R s =.8,.9 min HPLC: µm C8.9 mins Excel C8 0 x. mm 0. ml/min, t G = min max pressure: bar 9 min cycle time Excel C8-PFP 0 x. mm. ml/min, tg = 0. min max pressure: 9 bar min cycle time 0 0 R s =.8 0 R s =., 0. min R s =.9 R s =. 0.. R s =. UHPLC: µm C8-PFP 0. mins,. min UHPLC: <µm C8. mins... ~ x Quicker min R s. 0 0 0, aspirin;, phenacetin;,,-dinitrobenzene ;, ethylbenzoate;, nimesulide;, ibuprofen;, indomethacin. min Australia & New Zealand contact: infor@winlab.com.au or call + 0
Excel Has Typically Lower Back Pressure For UHPLC Specifically engineered for lower UHPLC backpressures Excel C8-AR 8 9 0 P max : bar Waters ACQUITY.um BEH C8 8 0 9 P max : 8 bar Phenomenex Kinetex.um C8, 0 8 9 P max : 0 bar Agilent ZORBAX Eclipse.8 um XDB C8, 8 0 9 P max : 0 bar 0..... min 0x.mm; conditions: A=mM formic acid (aq); B=mM formic acid in MeOH; tg= to 00%B in min; 0. ml/min; 0C; nm. Paracetamol. Hydrochlorothiazide. Methylphenylsulphoxide. Methylphenylsulphone. Aspirin. Phenacetin.,-dinitrobenzene 8.,,-trimethoxybenzene 9. Ethylbenzoate 0. Nimesulide. Ibuprofen. Indomethacin. Mefenamic acid All trademarks are recognised Australia & New Zealand contact: infor@winlab.com.au or call + 0
Excel UHPLC Columns Scalability & Reproducibility Excel C8 0 x.mm 0.mL/min µm UHPLC C8 0 x.0mm 0.0mL/min µm HPLC C8 0 x.mm.00ml/min µm 0 C8 0 x.mm.ml/min 0 µm 0 8 Prep LC MP: : v/v MeCN:0.% TFA (aq); C; nm;. uracil;. -hydroxybenzoic acid;. acetylsalicyclic acid;. benzoic acid;. -hydroxybenzoic acid. ethyl paraben Australia & New Zealand contact: infor@winlab.com.au or call + 0
Excel UHPLC Column Robustness 000 bar for ~000 gradient runs Isocratic efficiency assessments every ~00 runs more demanding! Excel C8 (with HSC Packing Technology) Prototype mm C8 (without HSC Packing Technology) 00x.mm; MPA 0.% FA (aq); MPB: 0.% FA in MeOH; 0.mL/min; gradient: 0-90%B in mins, min hold; min re-equilibration. NEW High Stability Column (HSC ) Packing Technology Significantly Improves UHPLC Column Robustness 8 Australia & New Zealand contact: infor@winlab.com.au or call + 0
Excel UHPLC Columns ph and Temperature Stability: For optimal column lifetime, as with standard columns, the recommended ph operating range is -8 Excel columns can be used outside of this ph range but column lifetimes will vary with a number of factors (buffer, temperature, solvents etc)* Excel columns can be operated at temperatures up to 00 C (but temperatures >0 C will reduce column lifetimes) * For further information see FREE booklet: A Guide to HPLC and LC-MS Buffer Selection Australia & New Zealand contact: infor@winlab.com.au or call + 0
Excel C8-AR: Water Soluble Vitamins - Selectivity Excel C8-AR P max : 09 bar, Excel C8 P max : bar Agilent ZORBAX Eclipse XDB-C8 P max : 99 bar Waters ACQUITY BEH C8 P max : bar Phenomenex Kinetex. C8, P max : bar 0x.mm; MP A: 0 mm KH PO, ph.; MPB: 0 mm KH PO, ph. in MeOH/H O (0:0 v/v); Gradient: 0 0 %B in min; 0. ml/min; 0 C; 0 nm. Pyridoxine;. P-aminobenzoic acid;. Pantothenic acid;. Folic acid;. D-Biotin;. Cyanocobalamin;. Riboflavin min Australia & New Zealand contact: infor@winlab.com.au or call + 0
Excel C8-PFP: Structural Isomers - Selectivity Excel C8-PFP P = bar,, Excel C8, P = 0 bar,, Waters. ACQUITY BEH C8, P = bar,, Phenomenex Kinetex. C8, P = bar,, Agilent Poroshell 0 EC-C8, P = 08 bar 8 9 min 0x.mm; MP: MeOH/H O (0:0 v/v); 0. ml/min; 0 C; 0/ nm;.,,-trimethoxybenzene;.,-dimethoxybenzene;.,,-trimethoxybenzene;. methoxybenzene;.,-dimethoxybenzene;.,,-trimethoxybenzene;. toluene Australia & New Zealand contact: infor@winlab.com.au or call + 0
Excel UHPLC Columns A reusable UHPLC column connector is available EXL-CC EXL-CC0 = / pk = 0 / pk Rated to 0 bar (,000 psi), these are suitable for at least 0 make / break cycles when correctly fitted Suitable for use at temperature up to 00C A torque wrench is recommended to ensure the correct force is applied EXL-TW = / pk Australia & New Zealand contact: infor@winlab.com.au or call + 0
Excel UHPLC Columns A starter kit containing x Torque Wrench and x reusable connectors is available + + + + EXL-CCSK