Preparative Chromatography Meets UHPLC

Preparative Chromatography Meets UHPLC The Power of Kinetex Core-Shell Technology with the Performance of Piston Packed Prep Columns Ultra-High Efficiencies Ultra-Long Lifetimes NO Added Pressure

Two Award Winning Technologies Award Recipient AXIA Award Recipient Kinetex NOW COMBINED to give you unmatched purification performance for both HPLC and SFC applications. Core-Shell Technology Kinetex Core-Shell Technology produces increased efficiencies over traditional, fully porous columns, yielding remarkable chromatographic resolution, higher peak capacities, and greater sensitivity, so labs can get even more out of their HPLC analyses! The benefits of Kinetex Core-Shell Technology include: Increased efficiencies over traditional fully porous columns Seamless scalability from HPLC/UHPLC to Preparative LC Kinetex µm provides better performance than traditional fully porous and µm materials. µm.7 µm.6 µm µm Preparative Column Packing Technology An advanced preparative column packing and hardware design, Axia incorporates patented Hydraulic Piston Compression technology that offers increased sorbent bed density and eliminates media bed collapse as a source of premature column failure in preparative HPLC columns. Unlike traditional column packing methods, the Axia packing method is completely automated and monitored by multiple sensors to allow for measurement and recording of all process parameters for every column. The result is a vastly improved packing process that offers the following benefits: Extended column lifetimes Improved reproducibility: Column-to-Column and Batch-to-Batch Efficiencies and peak symmetries on par with analytical separations Increased column stability under high flow rates

Combining These Two Award Winning Technologies Gives You Higher Efficiency! Start with sharper peaks by taking advantage of the high efficiencies of Kinetex µm Axia preparative columns. Waters XBridge µm C8 Prep OBD Kinetex µm XB-C8 Axia Packed Response - Millivolts 4 x 9. mm N =, plates App ID 46 Response - Millivolts 4 x. mm N = 8,4 plates Over % Increase in Efficiency App ID 4 4 6 8 4 min 4 6 8 4 min Conditions for both columns: Columns: Kinetex µm XB-C8 Axia Packed XBridge µm C8 Prep OBD Dimensions: x. mm (Kinetex) x 9 mm (XBridge) Mobile Phase: Water/ Acetonitrile (:) Injection Volume: µl Flow Rate: ml/ min Temperature: Ambient Detection: UV @ 4 nm Sample:. Uracil. Acetophenone. Toluene 4. Naphthalene Excellent Loadability! With narrower peak widths than fully porous columns across every sample load, Axia packed Kinetex µm columns give you the capability of increased sample load and higher throughput for vastly improved purification performance and economics. 77 Waters XBridge µm C8 Prep OBD mv 6 6 4 4 4 6 7 8 9 min Conditions for both columns: Columns: Kinetex μm C8 Axia Packed XBridge µm C8 Prep OBD Dimensions: x. mm (Kinetex) x 9 mm (XBridge) Mobile Phase: A: Water with. % Formic acid B: Acetonitrile with. % Formic acid Gradient: Time (min) 8 % B App ID 77 mv 6 6 4 4 Kinetex µm C8 Axia Packed Increased Sensitivity and Excellent Loadability! 4 6 7 8 9 min Flow Rate: ml/min Temperature: Ambient Detection: UV @ 4 nm Sample: mg/ml in DMSO. Doxepin (From - mg on-column). Amitriptyline (From - mg on-column) App ID 774 Waters is a registered trademark of Waters Corp. OBD and XBridge are trademarks of Waters Corp. Phenomenex is in no way affiliated with Waters Corp. Comparative separations may not be representative of all applications.

Preparative Technology Redefined Axia Technology Vs. Traditional Prep Column Packing Waters OBD Patented Prep Column Packing Process: TRADITIONAL PREP PACKING In traditional slurry packing processes, like the Waters OBD (Optional Bed Density) Prep column packing approach, pressure on the packed bed is released when the column is removed from the column packing station to allow attachment of the endfitting. This conventional packing process involves: Compression Decompression Recompression Several problems with this packing method are: Variability in column performance due to increased number of manual operations required for assembly Potential silica media damage during recompression Limited level of process control is based on traditional slurry packing technology Pump Column Media escapes from disconnected column Silica media packed under liquid pressure Removal of escaped media Violent recompression of silica media Media density disruption and potential media crushing Diagram from Waters Corporation U.S. Patent No. 7,99,4 Our Vastly Improved Patented Packing Process: AXIA TECHNOLOGY In contrast to the multi-step process required in conventional slurry packing, Axia packed preparative columns are packed using a single axial compression step. The ideal column bed density is custom calculated and automated for each specific media and column size. Computer control of the entire process ensures both proper bed density and column uniformity every time. During the Axia packing process, the packing piston is locked in place, eliminating any de compression and recompression of the packed bed. This improves media integrity and column bed stability, and solves the primary lifetime and performance problems associat ed with conventional slurry packed preparative columns. Silica media packed under piston pressure Column Specialized computer control for ideal pressure Packing piston locked. No media or pressure escape Media maintains initial optimal packing density and shape Axia patent: U.S. Patent No. 7,674,8 Waters is a registered trademark of Waters Corp. OBD is a trademark of Waters Corp. Phenomenex is in no way affiliated with Waters Corp. 4

The Packing Difference You Can See! Traditional Prep Packing Decompression and then recompression during packing can damage the media and lead to increased column-to-column variability, flow disturbances, and decreased column lifetimes. *SEM of Waters OBD inlet frit Crushed media or silica fines at frit surface after packing Axia Packing Technology Highly tuned patented process and hardware eliminates potential decompression and ensures media stability and optimal bed density. *SEM of Axia inlet frit Intact media at frit surface after packing *The images are believed to be representative, but individual columns may vary. View an animated packing process comparison www.axiaprep.com Unmatched Column Reproducibility The completely automated Axia packing system provides feedback control and infinite tuning of packing density for specific media characteristics such as mechanical strength and porosity. An optimum bed density can be consistently reproduced column-to-column. This directly translates into consistent efficiency and peak asymmetry measurements and decreases the column variability seen in traditionally packed preparative columns. Efficiency (N),, 8, Reproducible Column-to-Column Efficiency Average Efficiency (N) with Synergi 4 µm Hydro-RP x. mm.8 % RSD. 7 % Improved Avg. Efficiency Peak Asymmetry.4.. Reproducible Column-to-Column Peak Asymmetry Average Peak Asymmetry with Gemini µm C8 x. mm 9. % RSD % Improved Avg. Peak Shape. Density (%) 6 6 6 9 8 7 6 Density Comparison of Packed Beds 9 % Increase in Packing, density and More Uniformly Packed Bed 6,. 4 4, Traditional Slurry Packing Axia Packed Hydraulic Piston Compression Packing Traditional Slurry Packing Axia Packed Hydraulic Piston Compression Packing Traditional Slurry Packing Axia Packed Hydraulic Piston Compression Packing Waters is a registered trademark of Waters Corp. OBD is a trademark of Waters Corp. Phenomenex is in no way affiliated with Waters Corp. Comparative separations may not be representative of all applications.

Seamless Scalability from HPLC/UHPLC to PREP The recent addition of the Kinetex µm in the Axia packed format (. mm ID) makes it the first core-shell sorbent commercially available for small-scale preparative applications. Combine this with the added flexibility that the entire Kinetex core-shell line (. µm,.7 µm,.6 µm and μm) is fully scalable in retention and selectivity, makes transferring high performance HPLC/ UHPLC methods to preparative and SFC applications, simple. Analytical method Kinetex.6 μm XB-C8 mau 8 6 4 App ID 4 Column: Kinetex.6 µm XB-C8 Dimensions: x 4.6 mm Part No.: F-4496-E Mobile Phase: A:.% TFA in Water B:.% TFA in Acetonitrile Gradient: Linear 8: (A/B) to :9 (A/B) over minutes Injection Volume: µl Flow Rate: ml/ min Temperature: Ambient Detection: UV @ nm Sample: Crude peptide mix 4 6 8 min Preparative scale-up and fraction collection Kinetex μm XB-C8 mv 7 7 FR FR FR FR 4 App ID 44 Column: Kinetex µm XB-C8 Axia Packed Dimensions: x. mm Part No.: F-46-P-AX Mobile Phase: A:.% TFA in Water B:.% TFA in Acetonitrile Gradient: Linear 8: (A/B) to :9 (A/B) over minutes Injection Volume: ml Flow Rate: ml/ min Temperature: Ambient Detection: UV @ nm Sample: Crude peptide mix........ 4. 4... 6. 6. 7. 7. 8. 8. 9. 9. min Analytical fraction analysis Kinetex.6 μm XB-C8 Fraction = 97. % Purity Fraction 4 Fraction Fraction Fraction App ID 4 Column: Kinetex.6 µm XB-C8 Dimensions: x 4.6 mm Part No.: F-4496-E Mobile Phase: A:.% TFA in Water B:.% TFA in Acetonitrile Gradient: Linear 8: (A/B) to :9 (A/B) over minutes Injection Volume: µl Flow Rate: ml/ min Temperature: Ambient Detection: UV @ nm Sample: Crude peptide mix. 4 4.. min Tip: For more information on the power of Kinetex core-shell scalability, request technical note TN- at: www.phenomenex.com/kinetex/axiarequest 6

A Broad Spectrum of Column Selectives Kinetex core-shell columns are available in a wide range of stationary phases, allowing you to optimize your separation for maximum resolution and loadability across HPLC, UHPLC, and Preparative HPLC and SFC applications. 9 4 C8 6 4 App ID 9 8 7 6 9 9 min C8 min Endcapped C8 phase Increased retention for polar basic compounds XB-C8 6 4, 8 4 7 69 Si App ID 9 XBC8 min min Protective isobutyl side chains Increased retention of polar acidic compounds 86, 6 4 Phenyl-Hexyl App ID 994 6,4 8 7 9 96 PhenylHexyl Phenyl-Hexyl Greater retention and separation of aromatic hydrocarbons min F,4 F PFP 6 F F F App ID 99 8 7 6 4 Conditions for all columns: Column: Kinetex.6 µm C8 Kinetex.6 µm XB-C8 Kinetex.6 µm Phenyl-Hexyl Kinetex.6 µm PFP Dimensions: x. mm Mobile Phase: A:.% Formic acid in Water B:.% Formic acid in Acetonitrile Gradient: Time (min) %B.. 4. 9 4.. 9 Flow Rate: Temperature: Detection: Sample: PFP Pentafluorophenyl phase Unique aromatic and polar selectivity min.8 ml/min ºC UV @ 4 nm (ambient). Pyridine. Triprolidine. Acetaminophen. Nortriptyline. Pindolol. Prednisolone 4. Quinidine. -Methyl. Sulfathiazole 4-nitrobenzoic acid 6. Acebutolol 4. -Hydroxy7. Benzyl alcohol -methylbenzaldehyde 8. Chlorpheniramine. Diflunisal 9. Phenol 6. Hexanophenone red-acids blue-bases white-neutrals Comparative separations may not be representative of all applications. Columns are ph stable from.- under isocratic conditions. Columns are ph stable.-8. under gradient conditions. Phenomenex l WEB: www.phenomenex.com 7

A New Era of Technical Support Services, Let Us Do the Work for You PhenoLogix, our in-house application support lab, saves you time and money by screening multiple scout columns and solvent strategies for new purification methods or revalidating your current methods. We work together to make you successful by minimizing your process purification development time and optimizing your purification method. Column Screening Normal Phase Reversed Phase Polar Organic SFC Chiral Method Optimization Services Fast Turnaround Easy Method Transfer Continued Support Preparative and Process Scale-Up Media Screening Small Scale Purification DAC Packing Assistance Get started today and let us exceed your expectations. www.phenomenex.com/phenologix 8

Extend the Lifetime of Your Axia Preparative Column Use the SecurityGuard PREP column protection system! Extend preparative column lifetime by as much as x Protect column from samples that precipitate out of solution Protect column from contaminants Stable and leak-free up to 6 ml/min The SecurityGuard PREP system was designed to effectively (and inexpensively) protect your valuable Prep columns from the damaging effects of mobile phase and sample chemical contaminants and particulates, without altering your chromatographic results. Forced Degradation Lifetime Study Axia packed column with SecurityGuard PREP cartridge after initial injection Injection - 4 min Conditions Column: Luna µm C8() Axia Packed Dimension: x. mm Part No.: B-4-P-AX Mobile Phase: A:.% TFA in Water B:.% TFA in Water/Acetonitrile (:7) Gradient: Linear 9:7 (A/B) to % B over minutes Injection Volume: 4 µl Flow Rate: 6 ml/min Temperature: Ambient Detection: UV @ 7 nm Sample:. Nadolol. Metoprolol. Propranolol Axia packed column with SecurityGuard PREP cartridge after multiple injections Injection 4-4 min Time to change the PREP cartridge Axia packed column after removing SecurityGuard column protection system Injection 4 Original column performance maintained by using SecurityGuard PREP SecurityGuard PREP - 4 min 9

If Axia packed columns do not provide at least an equivalent separation as compared to a competing preparative column of the same particle size, same phase and dimensions, return the column with comparative data within 4 days for a FULL REFUND. Only applies to. mm ID columns.

Ordering Information Kinetex Analytical Columns SecurityGuard SecurityGuard µm Columns (mm) ULTRA Cartridges* ULTRA Cartridges* x. /pk x 4.6 x 4.6 x 4.6 x 4.6 /pk XB-C8 B-46-AN AJ-878 B-46-E D-46-E F-46-E G-46-E AJ-8768 C8 B-46-AN AJ-878 B-46-E D-46-E F-46-E G-46-E AJ-8768 PFP B-46-AN AJ-8787 B-46-E D-46-E F-46-E G-46-E AJ-877 Phenyl-Hexyl B-46-AN AJ-8788 B-46-E D-46-E F-46-E G-46-E AJ-8774 for. mm ID for 4.6 mm ID SecurityGuard.6 μm Analytical Columns (mm) ULTRA Cartridges* x 4.6 x 4.6 7 x 4.6 x 4.6 x 4.6 /pk XB-C8 B-4496-E C-4496-E D-4496-E F-4496-E AJ-8768 C8 A-446-E B-446-E C-446-E D-446-E F-446-E AJ-8768 PFP A-4477-E B-4477-E C-4477-E D-4477-E F-4477-E AJ-877 Phenyl-Hexyl B-449-E C-449-E D-449-E F-449-E AJ-8774 for 4.6 mm ID SecurityGuard.6 μm MidBore Columns (mm) ULTRA Cartridges* x. x. 7 x. x. x. /pk XB-C8 A-4496-Y B-4496-Y C-4496-Y D-4496-Y F-4496-Y AJ-877 C8 A-446-Y B-446-Y C-446-Y D-446-Y F-446-Y AJ-877 PFP A-4477-Y B-4477-Y C-4477-Y D-4477-Y F-4477-Y AJ-878 Phenyl-Hexyl D-449-Y F-449-Y AJ-878 for. mm ID SecurityGuard.6 μm Minibore Columns (mm) ULTRA Cartridges* x. x. 7 x. x. x. /pk XB-C8 A-4496-AN B-4496-AN C-4496-AN D-4496-AN F-4496-AN AJ-878 C8 A-446-AN B-446-AN C-446-AN D-446-AN F-446-AN AJ-878 PFP A-4477-AN B-4477-AN C-4477-AN D-4477-AN F-4477-AN AJ-8787 Phenyl-Hexyl A-449-AN B-449-AN C-449-AN D-449-AN F-449-AN AJ-8788 for. mm ID SecurityGuard.7 μm MidBore Columns (mm) ULTRA Cartridges* x. x. x. /pk XB-C8 A-4498-Y B-4498-Y D-4498-Y AJ-877 C8 B-447-Y D-447-Y AJ-877 PFP B-4476-Y D-4476-Y AJ-878 for. mm ID SecurityGuard.7 μm Minibore Columns (mm) ULTRA Cartridges* x. x. x. x. /pk. µm Columns (mm) x. XB-C8 A-4498-AN B-4498-AN D-4498-AN F-4498-AN AJ-878 C8 A-447-AN B-447-AN D-447-AN F-447-AN AJ-878 PFP A-4476-AN B-4476-AN D-4476-AN F-4476-AN AJ-8787 Phenyl-Hexyl B-4-AN D-4-AN F-4-AN AJ-8788 for. mm ID C8 B-4-AN Axia Packed Kinetex Preparative Columns SecurityGuard µm Axia Packed Preparative Columns (mm) PREP Cartridges** x. x. x. x. x. /ea XB-C8 B-46-P-AX D-46-P-AX F-46-P-AX G-46-P-AX AJ-94 C8 B-46-P-AX D-46-P-AX F-46-P-AX G-46-P-AX AJ-94 PFP B-46-P-AX D-46-P-AX F-46-P-AX G-46-P-AX AJ-946 Phenyl-Hexyl B-46-P-AX D-46-P-AX F-46-P-AX G-46-P-AX AJ-947 for. mm ID SecurityGuard Column Protection Protect your Kinetex and Axia Packed preparative columns from the damaging effects of mobile phase and sample chemical contaminants with SecurityGuard ULTRA and SecurityGuard PREP Dramatically extends column lifetime and performance Virtually no change in chromatography Simple to use *SecurityGuard ULTRA cartridges require holder, Part No. AJ-9 **SecurityGuard PREP cartridges require holder, Part No. AJ-8 SecurityGuard ULTRA Part No. Description Unit Price AJ-9 SecurityGuard ULTRA Holder for UHPLC/ HPLC Columns. to 4.6mm ID ea SecurityGuard PREP Part No. Description Unit Price AJ-8 SecurityGuard PREP HPLC Guard Cartridge Holder Kit,.mm ID, includes column coupler ea Phenomenex l WEB: www.phenomenex.com

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