Breeding in the past. Seedling were the main source for the cultivars today. Spontaneous mutations on branches were used to select new cultivars.

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Transcription:

Origin of citrus

Breeding in the past Seedling were the main source for the cultivars today. Spontaneous mutations on branches were used to select new cultivars.

Present breeding The current breeding projects are based on crossing and Selection: 1.Crossing selecion and inducing mutation for seedlessness. 2.Crossing for seedless triploids.

ARO Citrus Breeding Program A. New flavors and colors B. Easy peeling C. Early and Late Ripening Seasons D. Seedlessness C. High yield and low alternate bearing- more then 30 tons per hectare E. Long shelf life F. Pests resistance G. Dwarfing

INDUCING MUTATIONS A technique used to thoroughly extract seeds Before (with seeds): After (seedles): SEEDLESS

Breeding in the future Developing molecular markers for important traits such as: ripening season, alternaria, seedlessness, etc. The selection will start at the net house by sampling DNA from the seedling following by PCR. Developing biotechnology approaches for transforming genes of important traits into citrus cultivars.

Current projects A. Developing molecular markers for parents identification and alternaria resistance. B. Transformation of genes for drought resistance.

Germinating in the dark in sterile condition

Infecting with agrobacterium

Grafting the trasformants

Growing in transgenic greenhouse

Expression of reporting gene in the stomata

KEDEM Ripening Season - September-November Total soluble solids (TSS): 14.9% Acid content: 1.02% TSS/acid ratio: 14.55 alternaria: resistant - Seedless - Early ripening

Rishonit RIPENING SEASON - OCTOBER-DECEMBER TOTAL SOLUBLE SOLIDS (TSS): 16.3% ACID CONTENT: 1.0% TSS/ACID RATIO: 16.3 alternaria: resistant - Seedless - Early ripening

ORIT Ripening Season January-February Total soluble solids (TSS): 15.6% Acid content: 1% TSS/acid ratio: 15.6 alternaria: resistant - Seedless - Late ripening

Admoni Ripening Season September - October Total soluble solids (TSS): 13.27% Acid content: 1.6% TSS/acid ratio: 8.3 - Early ripening

RIPENING SEASON - NOVEMBER TOTAL SOLUBLE SOLIDS (TSS): 13.5% ACID CONTENT: 1.1% TSS/ACID RATIO: 12.3 alternaria: resistant Tami - Seedless - Early ripening --Dwarf Tree

Dity RIPENING SEASON - NOVEMBMBER TOTAL SOLUBLE SOLIDS (TSS): 12.8% ACID CONTENT: 1.57% TSS/ACID RATIO: 8.15 alternaria: susceptible - Seedless

RIPENING SEASON: DECEMBER-JANUARY TOTAL SOLUBLE SOLIDS (TSS): 17.5% ACID CONTENT: 1.34% TSS/ACID RATIO: 13 alternaria: susceptible Meirav - Seedless -Long Shelf-life

Ripening Season: December-January Total soluble solids (tss):15.5% Acid content:1.1% TSS/acid ratio: 14.09 NECTAR - Seedless -Long Shelf-life

RIPENING SEASON: FEBRUARY-MARCH TOTAL SOLUBLE SOLIDS (TSS): 14.27% ACID CONTENT: 1.54% TSS/ACID RATIO: 9.26 alternaria: resistant YIFAT - Seedless --Long Shelf-life

RIPENING SEASON: FEBRUARY-MARCH TOTAL SOLUBLE SOLIDS (TSS): 14.1% ACID CONTENT: 2.24% TSS/ACID RATIO: 6.3 alternaria: susceptible WINOLA - Seedless --Long Shelf-life

RONIT RIPENING SEASON: FEBRUARY- MARCH TOTAL SOLUBLE SOLIDS (TSS): 18.8% ACID CONTENT: 1.2% TSS/ACID RATIO: 15.67 alternaria: resistant - Seedless --Long Shelf-life

ODEM RIPENING SEASON: JANUARY- FEBRUARY TOTAL SOLUBLE SOLIDS (TSS): 15% ACID CONTENT: 0.96% TSS/ACID RATIO: 15.62 alternaria: resistant - Seedless --Long Shelf-life

RIPENING SEASON: JANUARY-FEBRUARY TOTAL SOLUBLE SOLIDS (TSS): 15.8% ACID CONTENT: 1.2% TSS/ACID RATIO: 13.16 alternaria: resistant SIGAL

DANIT RIPENING SEASON: FEBRUARY-MARCH TOTAL SOLUBLE SOLIDS (TSS): 14.8% ACID CONTENT: 1.28% TSS/ACID RATIO: 11.56 alternaria: resistant - Seedless --Long Shelf-life

MOR- SEEDLESS MURCOTT RIPENING SEASON: JANUARY-MARCH TOTAL SOLUBLE SOLIDS (TSS): 15% ACID CONTENT: 1.3% TSS/ACID RATIO: 11.54 alternaria: susceptible - Seedless --Long Shelf-life

ORRI RIPENING SEASON: JANUARY-APRIL TOTAL SOLUBLE SOLIDS (TSS): 14% ACID CONTENT: 0.7% TSS/ACID RATIO: 20 alternaria: resistant - Seedless --Long Shelf-life

FLAMINGO Ripening Season: October-December Total soluble solids (TSS): 12.5% Acid content: 0.52 TSS/acid ratio: 24 - Seedless -High lycopene --Long Shelf-life

Aliza RIPENING SEASON: NOVEMBER-FEBRUARY TOTAL SOLUBLE SOLIDS (TSS): 15% ACID CONTENT: 0.64% TSS/ACID RATIO: 23.43 - Seedless --Long Shelf-life

EINAT RIPENING SEASON: NOVEMBER-FEBRUARY TOTAL SOLUBLE SOLIDS (TSS): 12.1% ACID CONTENT: 1.1% TSS/ACID RATIO: 11 - Seedless -High lycopene --Long Shelf-life

Ayelet-Seedless Villafranca Lemon RIPENING SEASON: ALL YEAR AROUND TOTAL SOLUBLE SOLIDS (TSS):7% ACID CONTENT: 9.6% TSS/ACID RATIO: 0.73 - Seedless --Long Shelf-life

Galya-Seedless Urica Lemon RIPENING SEASON: ALL YEAR AROUND TOTAL SOLUBLE SOLIDS (TSS):7% ACID CONTENT:9.8% TSS/ACID RATIO: 0.71 - Seedless --Long Shelf-life

1 RIPENING SEASON: JANUARY TOTAL SOLUBLE SOLIDS (TSS):12.4% ACID CONTENT:1.7% TSS/ACID RATIO: 7.3 - Seedless --Long Shelf-life

2 RIPENING SEASON: FEBRUARY TOTAL SOLUBLE SOLIDS (TSS):14% ACID CONTENT:1.3% TSS/ACID RATIO: 10.8 alternaria: resistant - Seedless --Long Shelf-life

3 RIPENING SEASON: JANUARY TOTAL SOLUBLE SOLIDS (TSS):14.8% ACID CONTENT:1.3% TSS/ACID RATIO: 11.4 alternaria: resistant - Seedless --Long Shelf-life

4 RIPENING SEASON: JANUARY TOTAL SOLUBLE SOLIDS (TSS):14.3% ACID CONTENT:1.1% TSS/ACID RATIO: 13 - Low seeds --Long Shelf-life

5 RIPENING SEASON: JANUARY TOTAL SOLUBLE SOLIDS (TSS):11.8% ACID CONTENT:1% TSS/ACID RATIO: 11.8 alternaria: resistant - Seedless --Long Shelf-life

6 RIPENING SEASON: JANUARY TOTAL SOLUBLE SOLIDS (TSS):11.9% ACID CONTENT:1.1% TSS/ACID RATIO: 10.8 alternaria: resistant - low Seeds --Long Shelf-life