LAMPIRAN A SERTIFIKAT PENGUJIAN STANDARISASI EKSTRAK ETANOL DAUN SALAM

LAMPIRAN A SERTIFIKAT PENGUJIAN STANDARISASI EKSTRAK ETANOL DAUN SALAM 81

LAMPIRAN B SERTIFIKAT PENGUJIAN STANDARISASI EKSTRAK ETANOL HERBA SAMBILOTO 82

LAMPIRAN C HASIL UJI PENETAPAN KADAR FLAVONOID TOTAL PADA EKSTRAK ETANOL SALAM-SAMBILOTO Rep. Abs. C obs (µg/ml) FP Csampel (µg/ml) W sampel (gram) Cteoritis (µg/ml) Kadar (%) I 0,593 6,681 10 66,807 1,0013 40052 0,167 II 0,651 7,284 10 72,843 1,0010 40040 0,182 III 0,502 5,734 10 57,338 1,0003 40012 0,143 Keterangan: FP = Faktor pengenceran Perhitungan 4d 0,143* 0,167 0,0075 0,1745 0,182 0,0075 + 0,0150 : 2 = 0,0075 4d = 4 x 0,0075 = 0,03 0,1745 0,143 > 0,03 0,0315 > 0,03 Data 0,143 di-reject Jadi, rata rata % kadar flavonoid total pada ekstrak adalah 0,175% 83

LAMPIRAN D HASIL UJI PENETAPAN KADAR FLAVONOID TOTAL PADA FRAKSI AIR SALAM-SAMBILOTO Rep. Abs. C obs (µg/ml) FP Csampel (µg/ml) W sampel (gram) Cteoritis (µg/ml) Kadar (%) I 0,475 5,453 66,667 363,526 1,0007 40028 0,908 II 0,485 5,557 66,667 370,463 1,0030 40120 0,923 III 0,502 5,734 66,667 382,256 1,0014 40056 0,954 Keterangan: FP = faktor pengenceran Perhitungan 4d 0,908 0,0075 0,9155 0,923 0,0075 + 0,954* 0,0150 : 2 = 0,0075 4d = 4 x 0,0075 = 0,03 0,954 0,9155 > 0,03 0,0385 > 0,03 Data 0,954 di-reject Jadi, rata rata % kadar flavonoid total pada fraksi air adalah 0,916% 84

LAMPIRAN E HASIL UJI MUTU FISIK GRANUL Mutu fisik yang diuji Kelembaban granul Bulk density Tapped density Hausner ratio Carr s index Formula tablet ekstrak etanol salamsambiloto Replikasi F I F II F III F IV I 3,74 3,31 3,23 4,28 II 4,83 4,83 4,33 3,67 4,29 4,07 3,78 3,98 SD 0,77 1,07 0,78 0,43 I 0,38 0,33 0,35 0,33 II 0,33 0,37 0,34 0,33 0,36 0,35 0,35 0,33 SD 0,04 0,03 0,01 0,00 I 0,44 0,39 0,41 0,39 II 0,39 0,43 0,40 0,39 0,42 0,41 0,41 0,39 SD 0,04 0,03 0,01 0,00 I 1,16 1,19 1,16 1,18 II 1,19 1,18 1,18 1,19 1,18 1,19 1,17 1,19 SD 0,02 0,01 0,01 0,01 I 14,00 16,00 14,00 15,50 II 16,00 15,00 15,00 16,00 15,00 15,50 14,50 15,75 SD 1,41 0,71 0,71 0,35 Persyaratan 3-5% (Voigt, 1995) - -,25 (Wells, 1988) 2 (Wells, 1988) 85

LAMPIRAN F HASIL UJI KESERAGAMAN BOBOT TABLET EKSTRAK SALAM-SAMBILOTO Hasil uji keseragaman bobot tablet formula I Replikasi I Replikasi II No. Bobot Penyimpangan Bobot Penyimpangan tablet (mg) (%) tablet (mg) (%) 1 703,1 0,22 703,2 0,06 2 701,9 0,39 700,7 0,29 3 707,1 0,35 703,5 0,11 4 705,9 0,18 700,5 0,32 5 706,0 0,20 700,8 0,28 6 703,6 0,14 701,6 0,16 7 700,7 0,56 703,6 0,12 8 703,9 0,10 704,2 0,21 9 705,6 0,14 701,2 0,22 10 707,6 0,42 703,2 0,06 11 704,3 0,04 704,7 0,28 12 705,5 0,13 700,2 0,36 13 704,2 0,06 701,3 0,21 14 702,3 0,33 701,8 0,14 15 705,9 0,18 705,6 0,40 16 702,8 0,26 702,1 0,09 17 708,6 0,57 704,3 0,22 18 704,7 0,01 706,1 0,48 19 703,3 0,19 701,3 0,21 20 705,3 0,10 705,2 0,35 704,62 702,76 SD 1,99 1,84 86

Hasil uji keseragaman bobot tablet formula II Replikasi I Replikasi II No. Bobot tablet (mg) Penyimpangan (%) Bobot tablet (mg) Penyimpangan (%) 1 701,5 0,33 704,1 0,24 2 699,7 0,07 704,5 0,30 3 700,4 0,17 701,7 0,10 4 698,1 0,16 700,7 0,24 5 695,2 0,57 701,3 0,15 6 702,5 0,47 699,2 0,45 7 699,1 0,01 701,8 0,08 8 700,1 0,13 701,5 0,13 9 695,1 0,58 704,8 0,34 10 697,5 0,24 704,1 0,24 11 697,4 0,26 702,4 0,00 12 701,4 0,32 702,5 0,02 13 698,8 0,06 701,3 0,15 14 702,2 0,43 700,5 0,27 15 696,4 0,40 702,7 0,04 16 700,1 0,13 702,8 0,06 17 700,2 0,14 703,5 0,16 18 697,8 0,20 704,7 0,33 19 699,4 0,03 702,2 0,03 20 700,9 0,24 701,4 0,11 699,19 702,39 SD 2,14 1,53 87

Hasil uji keseragaman bobot tablet formula III Replikasi I Replikasi II No. Bobot tablet (mg) Penyimpangan (%) Bobot tablet (mg) Penyimpangan (%) 1 709,1 0,39 701,1 0,51 2 709,9 0,50 704,1 0,08 3 708,9 0,36 708,6 0,55 4 700,2 0,87 710,2 0,78 5 704,4 0,28 705,4 0,10 6 707,6 0,17 707,5 0,40 7 704,1 0,32 703,1 0,23 8 700,8 0,79 705,3 0,09 9 707,6 0,17 705,8 0,16 10 709,6 0,46 702,7 0,28 11 708,4 0,29 705,2 0,07 12 705,8 0,08 700,1 0,65 13 704,4 0,28 701,0 0,52 14 708,8 0,34 704,7 0,00 15 709,2 0,40 703,8 0,13 16 704,0 0,34 703,5 0,17 17 707,0 0,09 702,5 0,31 18 709,0 0,37 707,6 0,41 19 704,7 0,24 704,5 0,03 20 703,9 0,35 707,1 0,34 706,37 704,69 SD 2,92 2,63 88

Hasil uji keseragaman bobot tablet formula IV Replikasi I Replikasi II No. Bobot Penyimpangan Bobot Penyimpangan tablet (mg) (%) tablet (mg) (%) 1 705,7 0,63 708,0 0,03 2 695,5 0,82 707,3 0,06 3 706,4 0,73 708,5 0,11 4 703,9 0,37 707,5 0,04 5 698,1 0,45 708,2 0,06 6 706,9 0,80 709,8 0,29 7 701,9 0,09 708,2 0,06 8 697,3 0,56 706,7 0,15 9 699,3 0,28 709,2 0,20 10 699,9 0,20 705,1 0,38 11 699,6 0,24 708,7 0,13 12 704,3 0,43 708,7 0,13 13 697,7 0,51 707,4 0,05 14 699,4 0,27 707,4 0,02 15 707,7 0,92 707,9 0,62 16 700,3 0,14 708,9 0,16 17 704,2 0,42 709,0 0,18 18 695,3 0,85 703,4 0,62 19 704,2 0,42 709,5 0,25 20 698,0 0,47 709,7 0,27 701,28 707,76 SD 3,86 1,85 89

LAMPIRAN G HASIL UJI KEKERASAN TABLET EKSTRAK ETANOL SALAM- SAMBILOTO No. Kekerasan tablet ekstrak etanol salam-sambiloto (kp) F I F II F III F IV Rep I Rep II Rep I Rep II Rep I Rep II Rep I Rep II 1 5,70 5,20 5,70 5,90 7,00 5,80 7,60 7,60 2 6,10 6,00 6,60 6,10 5,60 5,70 7,70 7,50 3 6,30 5,50 6,20 6,30 6,50 6,30 6,90 7,40 4 5,50 5,60 6,50 6,30 5,40 6,30 7,10 7,00 5 5,10 5,80 6,40 6,20 6,30 6,10 8,00 6,90 6 5,70 5,60 6,10 6,10 6,60 6,10 7,50 6,70 7 6,30 5,70 6,00 5,90 6,40 6,30 6,80 7,40 8 5,90 5,70 6,30 6,40 6,30 6,00 6,80 7,50 9 5,40 5,50 6,20 5,90 6,70 5,90 6,60 7,10 10 6,00 5,80 6,20 5,80 6,50 5,70 6,90 7,20 5,80 5,64 6,22 6,09 6,33 6,02 7,19 7,23 SD 0,37 0,22 0,26 0,21 0,49 0,24 0,47 0,30 90

LAMPIRAN H HASIL UJI KERAPUHAN TABLET EKSTRAK ETANOL SALAM- SAMBILOTO Formula Rep Berat awal (gram) Berat akhir (gram) Kerapuhan (%) Rata - rata SD KV (%) I II III IV I 14,14 14,08 0,46 II 14,05 13,99 0,45 I 14,00 13,95 0,36 II 14,08 14,02 0,43 I 14,14 14,08 0,45 II 14,06 14,00 0,44 I 14,04 13,98 0,39 II 14,20 14,15 0,39 0,46 0,00 0,96 0,39 0,05 12,47 0,44 0,00 0,86 0,39 0,00 0,85 91

LAMPIRAN I HASIL UJI WAKTU HANCUR TABLET EKSTRAK ETANOL SALAM-SAMBILOTO Formula Waktu hancur tablet ekstrak etanol salam-sambiloto (menit) Rata - rata Replikasi I Replikasi II SD KV (%) I 39,63 42,42 41,03 1,97 4,81 II 48,75 48,95 48,85 0,14 0,29 III 40,37 38,36 39,37 1,42 3,61 IV 43,33 42,20 42,77 0,08 1,87 92

LAMPIRAN J CONTOH PERHITUNGAN Contoh perhitungan Carr s index dan Hausner ratio: Formula I: Berat gelas ukur kosong (W 1 ) = 105,6253 gram Berat gelas ukur kosong + granul (W 2 ) = 143,7159 gram V 1 V 2 = 100 ml = 86 ml Bulk density = = 0,3809 Tapped density = = 0,4429 Carr s index = x 100% = x 100% = 14,00% Hausner ratio = = = 1,16 Contoh Perhitungan Kadar Flavonoid Total Fraksi Air dan Ekstrak Etanol Salam-Sambiloto Ekstrak etanol salam-sambiloto Rep. Abs. C obs (µg/ml) FP Csampel (µg/ml) W sampel (mg) Cteoritis (µg/ml) Kadar (%) I 0,593 6,681 10 66,807 1,0013 40052 0,167 93

perhitungan : Abs = 0,593 y= -0,0490 + 0,0961x C obs (x) = 6,681 µg/ml Csampel = C obs x FP = 6,681 x 10 = 66,807 µg/ml Cteoritis = = = 40052 µg/ml Kadar (%) = = x 100% = 0,167% Contoh Perhitungan Kadar Flavonoid Total dalam Tablet Ekstrak Etanol Salam-Sambiloto Rep. Abs. C obs (µg/ml) FP Csampel (µg/ml) W sampel (mg) Kadar total flavonoid (%) Bobot rata rata tablet (mg) Perolehan kembali (%) I 0,231 2,914 10 29,139 702,6 0,104 701 92,58 Perolehan kembali = x 100 % Perhitungan : Abs = 0,231 y= -0,0490 + 0,0961x C obs (x) = 2,914 µg/ml Csampel = C obs x FP = 2,914 x 10 = 29,139 µg/ml Perolehan kembali = x 100 % = 92,58% 94

LAMPIRAN K TABEL UJI r 95

LAMPIRAN L TABEL UJI F 96

LAMPIRAN M HASIL UJI STATISTIK KESERAGAMAN BOBOT TABLET ANTAR FORMULA N Mean Std. Deviation Descriptives Std. Error 95% Confidence Interval for Mean Lower Bound Upper Bound Minimum Maximum form 1 2 703,69000 1,315219 0,930000 691,87323 715,50677 702,760 704,620 form 2 2 700,79000 2,262742 1,600000 680,46007 721,11993 699,190 702,390 form 3 2 705,53000 1,187939 0,840000 694,85679 716,20321 704,690 706,370 form 4 2 704,52000 4,582052 3,240000 663,35190 745,68810 701,280 707,760 Total 8 703,63250 2,782567 0,983786 701,30622 705,95878 699,190 707,760 ANOVA: One Way Sum of squares df Mean square F Sig. Between groups 24,943 3 8,314 1,137 0,435 Within groups 29,256 4 7,314 total 54,199 7 Multiple comparisons Dependent variable: keseragaman bobot Tukey HSD Mean 95% confidence interval (I) (J) difference Std. Error Sig. formula formula Lower Upper (I-J) bound bound form 1 form 2 2,900000 2,704450 0,723-8,10943 13,90943 form 3-1,840000 2,704450 0,900-12,84943 9,16943 form 4-0,830000 2,704450 0,989-11,83943 10,17943 form 2 form 1-2,900000 2,704450 0,723-13,90943 8,10943 form 3-4,740000 2,704450 0,407-15,74943 6,26943 form 4-3,730000 2,704450 0,570-14,73943 7,27943 form 3 form 1 1,840000 2,704450 0,900-9,16943 12,84943 form 2 4,740000 2,704450 0,407-6,26943 15,74943 form 4 1,010000 2,704450 0,980-9,99943 12,01943 form 4 form 1 0,830000 2,704450 0,989-10,17943 11,83943 form 2 3,730000 2,704450 0,570-7,27943 14,73943 form 3-1,010000 2,704450 0,980-12,01943 9,99943 98

LAMPIRAN N HASIL UJI STATISTIK KEKERASAN TABLET ANTAR FORMULA N Mean Std. Deviation Std. Error Descriptive 95% Confidence Interval for Mean Lower Bound Upper Bound Minimum Maximum form 1 2 5,7200 0,11314 0,08000 4,7035 6,7365 5,64 5,80 form 2 2 6,1550 0,09192 0,06500 5,3291 6,9809 6,09 6,22 form 3 2 6,1750 0,21920 0,15500 4,2055 8,1445 6,02 6,33 form 4 2 7,2100 0,02828 0,02000 6,9559 7,4641 7,19 7,23 Total 8 6,3150 0,59409 0,21004 5,8183 6,8117 5,64 7,23 ANOVA: One Way Sum of squares df Mean square F Sig. Between groups 2,401 3 0,800 45,659 0,001 Within groups 0,070 4 0,018 total 2,471 7 Dependent variable: kekerasan Tukey HSD (I) formula (J) formula Mean difference (I-J) Multiple comparisons Std. Error Sig. 95% confidence interval Lower Upper bound bound form 1 form 2-0,43500 0,13238 0,096-0,9739 0,1039 form 3-0,45500 0,13238 0,084-0,9939 0,0839 form 4-1,49000 * 0,13238 0,001-2,0289-0,9511 form 2 form 1 0,43500 0,13238 0,096-0,1039 0,9739 form 3-0,02000 0,13238 0,999-0,5589 0,5189 form 4-1,05500 * 0,13238 0,005-1,5939-0,5161 form 3 form 1 0,45500 0,13238 0,084-0,0839 0,9939 form 2 0,02000 0,13238 0,999-0,5189 0,5589 form 4-1,03500 * 0,13238 0,005-1,5739-0,4961 form 4 form 1 1,49000 * 0,13238 0,001 -,9511 2,0289 form 2 1,05500 * 0,13238 0,005 0,5161 1,5939 form 3 1,03500 * 0,13238 0,005 0,4961 1,5739 *. The mean difference is significant at the 0,05 level. 99

LAMPIRAN O HASIL UJI STATISTIK KERAPUHAN TABLET ANTAR FORMULA N Mean Std. Deviatio n Std. Error Descriptives 95% Confidence Interval for Mean Lower Bound Upper Bound Minimum Maximum form 1 2 0,4550 0,00707 0,00500 0,3915 0,5185 0,45 0,46 form 2 2 0,3950 0,04950 0,03500-0,0497 0,8397 0,36 0,43 form 3 2 0,4450 0,00707 0,00500 0,3815 0,5085 0,44 0,45 form 4 2 0,3900 0,00000 0,00000 0,3900-0,3900 0,39 0,39 Total 8 0,4213 0,03643 0,01288 0,3908 0,4517 0,36 0,46 ANOVA: One Way Sum of squares df Mean square F Sig. Between groups 0,007 3 0,002 3,523 0,128 Within groups 0,003 4 0,001 total 0,009 7 Dependent variable: kerapuhan Tukey HSD Multiple comparisons Mean 95% confidence interval (I) (J) difference Std. Error Sig. formula formula Lower Upper (I-J) bound bound form 1 form 2 0,06000 0,02525 0,224-0,0428 0,1628 form 3 0,01000 0,02525 0,976-0,0928 0,1128 form 4 0,06500 0,02525 0,185-0,0378 0,1678 form 2 form 1-0,06000 0,02525 0,224-0,1628 0,0428 form 3-0,05000 0,02525 0,328-0,1528 0,0528 form 4 0,00500 0,02525 0,997-0,0978 0,1078 form 3 form 1-0,01000 0,02525 0,976-0,1128 0,0928 form 2 0,05000 0,02525 0,328-0,0528 0,1528 form 4 0,05500 0,02525 0,271-0,0478 0,1578 form 4 form 1-0,06500 0,02525 0,185-0,1678 0,0378 form 2-0,00500 0,02525 0,997-0,1078 0,0978 form 3-0,05500 0,02525 0,271-0,1578 0,0478 100

LAMPIRAN P HASIL UJI STATISTIK WAKTU HANCUR TABLET ANTAR FORMULA N Mean Std. Deviation Descriptives Std. Error 95% Confidence Interval for Mean Lower Bound Upper Bound Minimum Maximum form 1 2 41,0250 1,97283 1,39500 23,2998 58,7502 39,63 42,42 form 2 2 48,8500 0,14142 0,10000 47,5794 50,1206 48,75 48,95 form 3 2 39,3650 1,42128 1,00500 26,5953 52,1347 38,36 40,37 form 4 2 42,8750 0,64347 0,45500 37,0937 48,6563 42,42 43,33 Total 8 43,0288 3,94685 1,39542 39,7291 46,3284 38,36 48,95 ANOVA: One Way Sum of squares df Mean square F Sig. Between groups 102,783 3 34,261 20,857 0,007 Within groups 6,571 4 1,643 total 109,354 7 Multiple comparisons Dependent variable: waktu hancur Tukey HSD Mean 95% confidence interval (I) (J) Std. difference Sig. formula formula Error Lower Upper (I-J) bound bound form 1 form 2-7,82500 * 1,28165 0,012-13,0424-2,6076 form 3 1,66000 1,28165 0,611-3,5574 6,8774 form 4-1,74000 1,28165 0,580-6,9574 3,4774 form 2 form 1 7,82500 * 1,28165 0,012 2,6076 13,0424 form 3 9,48500 * 1,28165 0,006 4,2676 14,7024 form 4 6,08500 * 1,28165 0,030 0,8676 11,3024 form 3 form 1-1,66000 1,28165 0,611-6,8774 3,5574 form 2-9,48500 * 1,28165 0,006-14,7024-4,2676 form 4-3,40000 1,28165 0,172-8,6174 1,8174 form 4 form 1 1,74000 1,28165 0,580-3,4774 6,9574 form 2-6,08500 * 1,28165 0,030-11,3024-0,8676 form 3 3,40000 1,28165 0,172-1,8174 8,6174 *. The mean difference is significant at the 0,05 level. 101

LAMPIRAN Q HASIL UJI ANAVA KEKERASAN TABLET EKSTRAK ETANOL SALAM-SAMBILOTO DENGAN DESIGN EXPERT Response 1 kekerasan ANOVA for selected factorial model Analysis of variance table [Partial sum of squares - Type III] source Sum of Mean p-value df F value squares square Prob > F Model 2,40 3 0,80 45,66 0,0015 significant A-PVP K-30 1,08 1 1,08 61,65 0,0014 B-Crospovidone 1,14 1 1,14 65,05 0,0013 AB 0,18 1 0,18 10,27 0,0327 Pure error 0,070 4 0,018 Cor total 2,47 7 The Model F-value of 45.66 implies the model is significant. There is only a 0.15% chance that a "Model F-Value" this large could occur due to noise. Values of "Prob > F" less than 0.0500 indicate model terms are significant. In this case A, B, AB are significant model terms. Values greater than 0.1000 indicate the model terms are not significant. If there are many insignificant model terms (not counting those required to support hierarchy), model reduction may improve your model. Std. Dev. 0.13 R-Squared 0.9716 Mean 6.31 Adj R-Squared 0.9503 C.V. % 2.10 Pred R-Squared 0.8865 PRESS 0.28 Adeq Precision 15.917 The "Pred R-Squared" of 0.8865 is in reasonable agreement with the "Adj R-Squared" of 0.9503. 102

"Adeq Precision" measures the signal to noise ratio. A ratio greater than 4 is desirable. Your ratio of 15.917 indicates an adequate signal. This model can be used to navigate the design space. Factor Coefficient estimate df Standard error 95% CI low 95% CI high VIF Intercept 6,31 1 0,047 6,19 6,44 A-PVP K-30 0,37 1 0,047 0,24 0,50 1,00 B-Crospovidone 0,38 1 0,047 0,25 0,51 1,00 AB 0,15 1 0,047 0,020 0,28 1,00 Final Equation in Terms of Coded Factors: kekerasan = +6,31 +0,37 *A +0,38 *B +0,15 *A*B Final Equation in Terms of Actual Factors: kekerasan = +6,31500 +0,36750 * PVP K-30 +0,37750 * Crospovidone +0,15000 * PVP K-30 * Crospovidone The Diagnostics Case Statistics Report has been moved to the Diagnostics Node. In the Diagnostics Node, Select Case Statistics from the View Menu. 103

Proceed to Diagnostic Plots (the next icon in progression). Be sure to look at the: 1) Normal probability plot of the studentized residuals to check for normality of residuals. 2) Studentized residuals versus predicted values to check for constant error. 3) Externally Studentized Residuals to look for outliers, i.e., influential values. 4) Box-Cox plot for power transformations. If all the model statistics and diagnostic plots are OK, finish up with the Model Graphs icon. 104

LAMPIRAN R HASIL UJI ANAVA KERAPUHAN TABLET EKSTRAK ETANOL SALAM-SAMBILOTO DENGAN DESIGN EXPERT Response 2 kerapuhan ANOVA for selected factorial model Analysis of variance table [Partial sum of squares - Type III] source Sum of squares df Mean square F value p-value Prob > F Model 6,738E-003 3 2,246E-003 3,52 0,1277 A-PVP K-30 6,613E-003 1 6,613E-003 10,37 0,0323 B-Crospovidone 1,125E-004 1 1,125E-004 0,18 0,6960 AB 1,250E-005 1 1,250E-005 0,020 0,8954 Pure error 2,550E-003 4 6,375E-004 Cor total 9,288E-003 7 not significant The "Model F-value" of 3.52 implies the model is not significant relative to the noise. There is a 12.77 % chance that a "Model F-value" this large could occur due to noise. Values of "Prob > F" less than 0.0500 indicate model terms are significant. In this case A are significant model terms. Values greater than 0.1000 indicate the model terms are not significant. If there are many insignificant model terms (not counting those required to support hierarchy), model reduction may improve your model. Std. Dev. 0.025 R-Squared 0.7254 Mean 0.42 Adj R-Squared 0.5195 C.V. % 5.99 Pred R-Squared -0.0983 PRESS 0.010 Adeq Precision 3.641 105

A negative "Pred R-Squared" implies that the overall mean is a better predictor of your response than the current model. "Adeq Precision" measures the signal to noise ratio. A ratio of 3.64 indicates an inadequate signal and we should not use this model to navigate the design space. Factor Coefficient Standard 95% CI 95% CI df estimate error low high VIF Intercept 0,42 1 8,927E-003 0,40 0,45 A-PVP K-30-0,029 1 8,927E-003-0,054-3,965E- 003 1,00 B-Crospovidone -3,750E-003 1 8,927E-003-0,029 0,021 1,00 AB 1,250E-003 1 8,927E-003-0,024 0,026 1,00 Final Equation in Terms of Coded Factors: kerapuhan = +0,42-0,029 *A -3,750E-003 *B +1,250E-003 *A*B Final Equation in Terms of Actual Factors: kerapuhan = +0,42125-0,028750 *PVP K-30-3,75000E-003 *Crospovidone +1,25000E-003 *PVP K-30*Crospovidone The Diagnostics Case Statistics Report has been moved to the Diagnostics Node. In the Diagnostics Node, Select Case Statistics from the View Menu. 106

LAMPIRAN S HASIL UJI ANAVA WAKTU HANCUR TABLET EKSTRAK ETANOL SALAM-SAMBILOTO DENGAN DESIGN EXPERT Response 3 waktu hancur ANOVA for selected factorial model Analysis of variance table [Partial sum of squares - Type III] source Sum of Mean p-value df F value squares square Prob > F Model 102,78 3 34,26 20,86 0,0066 significant A-PVP K-30 63,00 1 63,00 38,35 0,0035 B-Crospovidone 29,99 1 29,99 18,26 0,0129 AB 9,79 1 9,79 5,96 0,0711 Pure error 6,57 4 1,64 Cor total 109,35 7 The Model F-value of 20.86 implies the model is significant. There is only a 0.66% chance that a "Model F-Value" this large could occur due to noise. Values of "Prob > F" less than 0.0500 indicate model terms are significant. In this case A, B are significant model terms. Values greater than 0.1000 indicate the model terms are not significant. If there are many insignificant model terms (not counting those required to support hierarchy), model reduction may improve your model. Std. Dev. 1.28 R-Squared 0.9399 Mean 43.00 Adj R-Squared 0.8949 C.V. % 2.98 Pred R-Squared 0.7597 PRESS 26.28 Adeq Precision 10.466 The "Pred R-Squared" of 0.7597 is in reasonable agreement with the "Adj R-Squared" of 0.8949. 108

"Adeq Precision" measures the signal to noise ratio. A ratio greater than 4 is desirable. Your ratio of 10.466 indicates an adequate signal. This model can be used to navigate the design space. Factor Coefficient Standard 95% CI 95% CI df estimate error low high VIF Intercept 43,00 1 0,45 41,74 44,26 A-PVP K-30 2,81 1 0,45 1,55 4,06 1,00 B-Crospovidone -1,94 1 0,45-3,19-0,68 1,00 AB -1,11 1 0,45-2,36 0,15 1,00 Final Equation in Terms of Coded Factors: waktu hancur = +43,00 +2,81 *A -1,94 *B -1,11 *A*B Final Equation in Terms of Actual Factors: waktu hancur = +43,00125 +2,80625 * PVP K-30-1,93625 * Crospovidone -1,10625 * PVP K-30 * Crospovidone The Diagnostics Case Statistics Report has been moved to the Diagnostics Node. In the Diagnostics Node, Select Case Statistics from the View Menu. 109

LAMPIRAN T HASIL UJI MUTU FISIK TABLET EKSTRAK SALAM- SAMBILOTO BERDASARKAN FORMULA OPTIMUM Keseragaman bobot No. Bobot tablet (mg) Replikasi I Penyimpangan (%) Bobot tablet (mg) Replikasi II Penyimpangan (%) 1 701,2 0,49 702,5 0,11 2 697,3 1,04 699,4 0,55 3 704,2 0,06 712,9 1,37 4 709,3 0,66 691,2 1,72 5 710,0 0,76 692,0 1,61 6 710,4 0,82 707,0 0,53 7 699,0 0,80 706,0 0,39 8 702,2 0,35 704,3 0,14 9 703,5 0,16 702,8 0,07 10 700,1 0,65 704,5 0,17 11 699,1 0,79 703,8 0,07 12 707,2 0,36 717,4 2,01 13 706,4 0,25 707,4 0,58 14 701,5 0,45 705,4 0,30 15 708,5 0,55 699,0 0,61 16 700,1 0,65 699,8 0,50 17 706,8 0,31 700,8 0,35 18 711,9 1,03 701,8 0,21 19 708,9 0,60 702,4 0,13 20 705,4 0,11 705,4 0,30 704,65 703,29 SD 4,42 5,94 111

Kekerasan tablet Kekerasan tablet ekstrak etanol salamsambiloto No. (kp) Replikasi I Replikasi II 1 6,00 6,30 2 5,50 6,90 3 5,80 5,80 4 5,40 6,50 5 5,90 6,00 6 6,20 6,30 7 6,00 5,90 8 6,20 6,20 9 5,80 6,30 10 5,70 6,50 5,85 6,27 SD 0,27 0,32 Kerapuhan tablet Replikasi Berat awal (gram) Berat akhir (gram) Kerapuhan (%) I 14,37 14,30 0,47 II 14,48 14,42 0,46 Rata - rata SD KV (%) 0,46 0,00 0,37 Waktu hancur tablet Replikasi Waktu hancur tablet ekstrak etanol salamsambiloto (menit) I 40,05 II 39,72 Rata - rata SD KV (%) 39,89 0,23 0,59 112

LAMPIRAN U TABEL UJI T 113

LAMPIRAN V HASIL UJI STATISTIK HASIL PERCOBAAN DAN HASIL TEORITIS PADA KEKERASAN TABLET EKSTRAK ETANOL SALAM-SAMBILOTO One-Sample Statistics N Mean Std. Deviation Std. Error Mean kekerasan 2 6,0600 0,29698 0,21000 t df One-Sample Test Sig. (2- tailed) Test Value = 6.17 Mean Difference 95% Confidence Interval of the Difference Lower Upper kekerasan -0,524 1 0,693-0,11000-2,7783 2,5583 114

LAMPIRAN W HASIL UJI STATISTIK HASIL PERCOBAAN DAN HASIL TEORITIS PADA KERAPUHAN TABLET EKSTRAK ETANOL SALAM-SAMBILOTO One-Sample Statistics N Mean Std. Deviation Std. Error Mean kerapuhan 2 0,4650 0,00707 0,00500 t df Sig. (2- tailed) One-Sample Test Test Value = 0.44 Mean Difference 95% Confidence Interval of the Difference Lower Upper kerapuhan 5,000 1 0,126 0,02500-0,0385 0,0885 115

LAMPIRAN X HASIL UJI STATISTIK HASIL PERCOBAAN DAN HASIL TEORITIS PADA WAKTU HANCUR TABLET EKSTRAK ETANOL SALAM-SAMBILOTO One-Sample Statistics N Mean Std. Deviation Std. Error Mean waktuhancur 2 39,8850 0,23335 0,16500 One-Sample Test Test Value = 39.61 t df Sig. (2- tailed) Mean Difference 95% Confidence Interval of the Difference Lower Upper waktuhancur 1,667 1 0,344 0,27500-1,8215 2,3715 116

LAMPIRAN Y HASIL PENETAPAN KADAR FLAVONOID TOTAL TABLET FORMULA OPTIMUM Rep. Abs. C obs (µg/ml) FP Csampel (µg/ml) W sampel (mg) Kadar flavonoid total (%) Bobot rata rata tablet (mg) Perolehan kembali (%) I 0,231 2,914 10 29,139 702,6 0,104 701 92,58 II 0,234 2,945 10 29,451 703,1 0,105 701 93,47 117

LAMPIRAN Z SERTIFIKAT ANALISIS BAHAN Ekstrak Etanol Salam 118

Ekstrak Etanol Sambiloto 119

Crospovidone 120

121

122

PVP K-30 123

124

Laktosa Monohidrat 125

126