Recombinant Integrins Ligand INTEGRIN α subunit S S β subunit
Recombinant Integrins Integrins are heterodimeric integral membrane proteins that play key roles in regulating cell-cell and cell-extracellular matrix interactions, which together contribute to tissue formation and initiate signaling pathways that control cell movement, differentiation, growth and survival. Twenty-four integrin α/β heterodimers have been identified. These heterodimeric proteins play important roles in cancer, fibrosis, and thrombotic and inflammatory diseases and therefore, have become a key subject of interest for pharmaceutical companies. R&D Systems offers a wide selection of Integrin-related research reagents. We are the only company that develops, tests, and sells active integrin heterodimers including popular pharmaceutical targets, such as Integrins α4β7, α2bβ3, αvβ1, αvβ3, αvβ5, and αvβ6 (details below). In addition, we offer a broad range of antibodies against integrin single chains and heterodimers, as well as a number of small molecules and peptides that act as integrin inhibitors or antagonists. A Subset of Bioactive Recombinant Human Integrin Proteins from R&D Systems Integrin a2bb3 Integrin avb3 Integrin avb6 2.5 4 2.5 Integrin α2bbβ3 Binding (Mean O.D.) 2 1.5 1 0.5 Vitronectin Binding (Mean O.D.) 3 2 1 Integrin αvβ6 Binding (Mean O.D.) 2 1.5 1 0.5 0 0.1 1 10 100 1000 Fibronectin (ng/ml) Recombinant Integrin a2bb3 Binds Fibronectin. Recombinant Human Integrin a2bb3 (Catalog # 7148-A2) was coated onto microplate wells at 2 µg/ml and the indicated concentrations of Human Fibronectin (Catalog # 1918-FN) were added. Fibronectin bound to Integrin a2bb3 in a dose-dependent manner with an apparent K D < 1 nm. kda 190 0 1 10 100 1000 10000 Integrin αvβ3 (ng/ml) Recombinant Integrin avb3 Binds Vitronectin. Recombinant Human Vitronectin (Catalog # 2308-VN) was coated onto microplate wells at 1 µg/ml and increasing concentrations of Recombinant Human Integrin avb3 (Catalog # 3050-AV) were added. Integrin avb3 bound to Vitronectin in a dose-dependent manner with an apparent K D < 10 nm. kda 190 0 0.01 0.1 1 10 100 LAP TGF-β1 (ng/ml) Recombinant Integrin avb6 Binds LAP TGF-b1. Recombinant Human Integrin avb6 (Catalog # 3817-AV) was coated onto microplate wells at 2 µg/ml and increasing concentrations of Recombinant Human LAP TGF-b1 (Catalog # 246-LP) were added. LAP TGF-b1 bound to Integrin avb6 in a dose-dependent manner with an apparent K D <0.1 nm. kda 190 92.5 66 55 43 Integrin α2bbβ3 92.5 66 55 43 36 Integrin αvβ3 92.5 66 55 43 Integrin αvβ6 36 29 21 18.4 12.4 6.3 29 21 18.4 12.4 6.3 36 29 18.4 21 12.4 6.3 SDS-PAGE Analysis of Recombinant Human Integrin a2bb3 under Reducing Conditions. To assess purity, Recombinant Human Integrin a2bb3 (Catalog # 7148-A2; 1 µg/lane) was resolved by 4 20% SDS-PAGE under reducing conditions and visualized by silver staining. SDS-PAGE Analysis of Recombinant Human Integrin avb3 under Reducing Conditions. To assess purity, Recombinant Human Integrin avb3 (Catalog # 3050-AV; 1 µg/lane) was resolved by 4 20% SDS-PAGE under reducing conditions and visualized by silver staining. SDS-PAGE Analysis of Recombinant Human Integrin avb6 under Reducing Conditions. To assess purity, Recombinant Human Integrin avb6 (Catalog # 3817-AV; 1 µg/lane) was resolved by 4 20% SDS-PAGE under reducing conditions and visualized by silver staining.
A Subset of Bioactive Recombinant Human Integrin Proteins from R&D Systems Integrin a4b7 Integrin avb5 Integrin avb1 60 3 2 % Cell Adhesion 50 40 30 20 10 Vitronectin Binding (Mean O.D.) 2 1 Integrin αvβ1 Binding (Mean O.D.) 1.5 1 0.5 0 0.01 0.1 1 10 100 Integrin α4β7 (µg/ml) Recombinant Integrin a4b7 Supports the Adhesion of VCAM-1-transfected Cells. Cultured Chinese hamster ovary (CHO) cells transfected with VCAM-1 were added to plates coated with increasing concentrations of Recombinant Human Integrin a4b7 (Catalog # 5397-A3). The cells adhered to immobilized Recombinant Human Integrin a4b7 in a concentration-dependent manner. 0 0.01 0.1 1 10 100 1000 Integrin αvβ5 (ng/ml) Recombinant Integrin avb5 Binds Vitronectin. Recombinant Human Vitronectin (Catalog # 2308-VN) was coated onto microplate wells at 1 µg/ml and increasing concentrations of Recombinant Human Integrin avb5 (Catalog # 2528-AV) were added. Integrin avb5 bound to Vitronectin in a dose-dependent manner with an apparent K D < 0.5 nm. 0 0.1 1 10 100 1000 10000 Fibronectin (ng/ml) Recombinant Integrin avb1 Binds Fibronectin. Recombinant Human Integrin avb1 (Catalog # 6579-AV) was coated onto microplate wells at 5 µg/ml and increasing concentrations of Human Fibronectin (Catalog # 1918- FN) were added. Fibronectin bound to Integrin avb1 in a dose-dependent manner with an apparent K D < 0.1 nm. kda 190 92.5 66 55 43 36 29 21 18.4 12.4 6.3 Integrin α4β7 kda 190 92.5 66 55 43 36 29 18.4 21 12.4 6.3 Integrin αvβ5 kda 190 92.5 66 55 43 36 29 21 18.4 12.4 6.3 Integrin αvβ1 SDS-PAGE Analysis of Recombinant Human Integrin a4b7 under Reducing Conditions. To assess purity, Recombinant Human Integrin a4b7 (Catalog # 5397-A3; 1 µg/lane) was resolved by 4 20% SDS-PAGE under reducing conditions and visualized by silver staining. SDS-PAGE Analysis of Recombinant Human Integrin avb5 under Reducing Conditions. To assess purity, Recombinant Human Integrin avb5 (Catalog # 2528-AV; 1 µg/lane) was resolved by 4 20% SDS-PAGE under reducing conditions and visualized by silver staining. SDS-PAGE Analysis of Recombinant Human Integrin avb1 under Reducing Conditions. To assess purity, Recombinant Human Integrin avb1 (Catalog # 6579-AV; 1 µg/lane) was resolved by 4-20% SDS-PAGE under reducing conditions and visualized by silver staining. Learn more rndsystems.com/integrins
Recombinant & Natural Proteins Protein Species Accession # Source Catalog # Size Human P56199 (Integrin α1) & P05556 (Integrin β1) CHO 7064-AB-025 25 µg Integrin a1b1 Mouse Q3V3R4(Integrin α1) & P09055 (Integrin β1) CHO 8188-AB-025 25 µg Human Q9UKX5 (Integrin α11) & P05556 (Integrin β1) CHO 6357-AB-050 50 µg Integrin a11b1 Mouse P61622 (α11 subunit) & P09055 (β1 subunit) CHO 7808-AB-050 50 µg Human NP_002194 (Integrin α2) & P05556 (Integrin β1) CHO 5698-A2-050 50 µg Integrin a2b1 Mouse Q62469.2 (Integrin α2) & P09055 (Integrin β1) CHO 7828-A2-050 50 µg Integrin a2bb3 Human P08514 (Integrin α2b) & AAA52589 (Integrin β3) CHO 7148-A2-025 25 µg Human P26006 (Integrin α3) & P05556 (Integrin β1) CHO 2840-A3-050 50 µg Integrin a3b1/vla-3 Mouse Q62470 (Integrin a3); P09055 (Integrin β1) coming soon Human P13612 (Integrin α4) & P05556 (Integrin β1) CHO 5668-A4-050 50 µg Integrin a4b1 Mouse AAH68313 (Integrin α4) & P09055 (Integrin β1) CHO 6054-A4-050 50 µg Human P13612 (Integrin α4); P26010 (Integrin β7) CHO 5397-A3-050 50 µg Integrin a4b7/lpam-1 Mouse NEW AAH68313 (Integrin α4); P26011 (Integrin β7) CHO 8615-A3-050 50 µg Human P08648 (Integrin α5) & P05556 (Integrin β1) CHO 3230-A5-050 50 µg Integrin a5b1 Mouse P11688 (Integrin α5) & P09055 (Integrin β1) CHO 7728-A5-050 50 µg Human NP_000201 (α6 subunit) & P05556 (β1 subunit) CHO 7809-A6-050 50 µg Integrin a6b1 Mouse Q61739 (α6 subunit) & P09055 (β1 subunit) CHO 7810-A6-050 50 µg Integrin a6b4 Mouse Q61739 (Integrin α6) & NP_598424 (Integrin β4) CHO 8067-A6-050 50 µg Integrin a6b4x1 isoform Human NP_000201 (Integrin α6) & P16144 (Integrin β4) CHO 5497-A6-050 50 µg Integrin a7b1 Mouse NP_032424(Integrin α7) & P09055 (Integrin β1) CHO 7958-A7-050 50 µg Human Q13797 (Integrin α9) & P05556 (Integrin β1) CHO 5438-A9-050 50 µg Integrin a9b1 Mouse CAC69080 (Integrin α9) & P09055 (Integrin β1) CHO 7826-A9-050 50 µg Human O75578 (Integrin α10) & P05556 (Integrin β1) CHO 5895-AB-050 50 µg Integrin a10b1 Mouse NP_001074522 (Integrin α10) & P09055 (Integrin β1) CHO 7827-AB-050 50 µg Human Q9UKX5 (Integrin α11) & P05556 (Integrin β1) CHO 6357-AB-050 50 µg Integrin a11b1 Mouse P61622 (α11 subunit) & P09055 (β1 subunit) CHO 7808-AB-050 50 µg Human P38570 (Integrin αe) & P26010 (Integrin β7) CHO 5850-A3-050 50 µg Integrin aeb7 Mouse NEW ABD49099 (Integrin αe), P26011 (Integrin b7) CHO 8356-A3-050 50 µg Human P20701 (Integrin αl) & P05107 (Integrin β2) CHO 3868-AV-050 50 µg Integrin alb2 Mouse P24063 (Integrin αl) & P11835 (Integrin β2) CHO 7825-AB-050 50 µg Human NP_001139280 (Integrin αm) & P05107 (Integrin β2) CHO 4047-AM-050 50 µg Integrin amb2 Mouse NP_032427 (Integrin αm) & P11835 (Integrin β2) CHO 7959-AM-050 50 µg Human NP_002201 (Integrin αv) & P05556 (Integrin β1) CHO 6579-AV-025 25 µg Integrin avb1 Mouse P43406 (Integrin αv) & P09055 (Integrin β1) CHO 7705-AV-050 50 µg Human NP_002201 (Integrin αv) & AAA52589 (Integrin β3) CHO 3050-AV-050 50 µg Integrin avb3 Mouse P43406 (Integrin αv) & O54890 (Integrin β3) CHO 7889-AV-050 50 µg Human P06756 (Integrin αv) & P18084 (Integrin β5) CHO 2528-AV-050 50 µg Integrin avb5 Mouse P43406 (Integrin αv) & NP_001139356 (Integrin β5) CHO 7706-AV-050 50 µg Human NP_002201 (Integrin αv) & P18564 (Integrin β6) CHO 3817-AV-050 50 µg Integrin avb6 Mouse P43406 (Integrin αv) & Q9Z0T9 (Integrin β6) CHO 7480-AV-050 50 µg Human P06756 (Integrin av) & P26012.1 (Integrin b8) CHO 4135-AV-050 50 µg Integrin avb8 Mouse P43406 (Integrin av) & Q0VBD0 (Integrin b8) CHO 8314-AV-050 50 µg Human P20702 (Integrin αx) & AAA59490 (Integrin β6) CHO 5755-AX-050 50 µg Integrin axb2 Mouse Q9QXH4 (Integrin αx) & P11835 (Integrin β2) CHO 7987-AX-050 50 µg
Antibodies Antibody Species Source Application(s) Catalog # Size B/N,, IHC, IP MAB3050 100 µg MAB3050-SP 25 µg Integrin avb3 FAB3050P FAB3050A FAB3050G FAB2528P MAB2528 100 µg B/N,, ICC/IF, IP Integrin avb5 MAB2528-SP 25 µg FAB2528A FAB2528G, ICC/IF, IP BAM1233 100 µg FAB1233P, ICC/IF, IP MAB1233 500 µg MAB1233-SP 25 µg Human Rat IgG 2A MAB12331 100 µg MAB12331-SP 25 µg MAB12332 100 µg Human Rat IgG 2A, ICC/IF Integrin a2/cd49b MAB12332-SP 25 µg AF1740 100 µg Mouse Sheep IgG, ICC/IF, AF1740-SP 25 µg Mouse Sheep IgG, ICC/IF, BAF1740 50 µg FAB1740A FAB1740P MAB1740 100 µg MAB1740-SP 25 µg, MAB7616 100 µg MAB7616-SP 25 µg FAB7616P FAB7616A Integrin a2b/cd41 MAB41181 100 µg, ICC/IF MAB41181-SP 25 µg MAB4118 100 µg MAB4118-SP 25 µg FAB41181P, ICC/IF BAM1345 100 µg, ICC/IF MAB1345 500 µg MAB1345-SP 25 µg FAB1345A Integrin a3/cd49c FAB1345F AF2787 100 µg Mouse Goat IgG, AF2787-SP 25 µg Mouse Goat IgG FAB2787P Mouse Goat IgG FAB2787A Mouse Goat IgG FAB2787G Learn more rndsystems.com/integrins
Antibodies continued Antibody Species Source Application(s) Catalog # Size, ICC/IF BAM1354 100 µg B/N BBA37 200 µg FAB1354A FAB1354P, ICC/IF MAB1354 500 µg MAB1354-SP 25 µg Integrin a4/cd49d FAB1354G FAB1354C MAB2450 100 µg MAB2450-SP 25 µg FAB2450A FAB2450P AF1864 100 µg Human Goat IgG B/N,, AF1864-SP 25 µg Human Goat IgG, BAF1864 50 µg FAB1864A FAB1864P, FA, ICC/IF MAB1864 100 µg MAB1864-SP 25 µg Integrin a5/cd49e MAB18642 100 µg MAB18642-SP 25 µg AF1754 100 µg Mouse Sheep IgG, AF1754-SP 25 µg, ICC/IF, MAB1754 100 µg MAB1754-SP 25 µg FAB1754P, IHC, IP MAB1350 500 µg MAB1350-SP 25 µg AF1350 100 µg Human Goat IgG AF1350-SP 25 µg Integrin a6/cd49f Human/Mouse/Bovine Rat IgG 2A FAB13501A Human/Mouse/Bovine Rat IgG 2A FAB13501P Human/Mouse/Bovine Rat IgG 2A B/N,, IHC MAB13501 100 µg MAB13501-SP 25 µg Human/Mouse/Bovine Rat IgG 2A FAB13501G MAB3518 100 µg MAB3518-SP 25 µg FAB3518A Integrin a7 FAB3518F FAB3518P FAB3518N FAB3518C
Antibody Species Source Application(s) Catalog # Size FAB6194P FAB6194A MAB6194 100 µg, ICC/IF, Integrin a8 MAB6194-SP 25 µg AF4076 100 µg Mouse Goat IgG, ICC/IF, AF4076-SP 25 µg Mouse Goat IgG, ICC/IF, BAF4076 50 µg MAB4574 100 µg MAB4574-SP 25 µg Integrin a9 AF3827 100 µg Mouse Goat IgG, AF3827-SP 25 µg Mouse Goat IgG FAB3827P AF4235 100 µg Human Goat IgG AF4235-SP 25 µg Integrin a11 Human/Mouse Rat IgG 1 MAB4235 100 µg MAB4235-SP 25 µg AF6498 100 µg Mouse Sheep IgG ICC/IF, AF6498-SP 25 µg AF1990 100 µg Mouse Goat IgG, AF1990-SP 25 µg Mouse Goat IgG, BAF1990 50 µg Integrin ae/cd103 Mouse Rat IgG 1 MAB1990 100 µg MAB1990-SP 25 µg Mouse Goat IgG FAB1990A Mouse Goat IgG FAB1990P Mouse Goat IgG FAB1990G, MAB3595 100 µg MAB3595-SP 25 µg FAB3595F FAB3595P Integrin al/cd11a FAB3595A FAB35951A FAB35951C MAB35951 100 µg MAB35951-SP 25 µg Learn more rndsystems.com/integrins
Antibodies continued Antibody Species Source Application(s) Catalog # Size, IHC BAM1699 100 µg FAB16991A-025 25 Tests FAB16991A-100 FAB16991P-025 25 Tests FAB16991P-100, ICC/IF, IHC MAB1699 500 µg MAB1699-SP 25 µg MAB16992 500 µg MAB16992-SP 25 µg FAB16991C-025 25 Tests FAB16991C-100 FAB16991G-025 25 Tests FAB16991G-100 FAB1699A-025 25 Tests FAB1699A-100 FAB1699P-025 25 Tests FAB1699P-100 FAB1699G-025 25 Tests FAB1699G-100 Human NEW Mouse IgG 2B FAB16991V-025 25 Tests FAB16991V-100 Human NEW Mouse IgG 2B FAB16991R-025 25 Tests FAB16991R-100 Human NEW Mouse IgG 2B FAB16991T-025 25 Tests FAB16991T-100 Integrin am/cd11b Human NEW Mouse IgG 2B FAB16991S-025 25 Tests FAB16991S-100 Human NEW Mouse IgG 2B FAB16991N-025 25 Tests FAB16991N-100 AF1699 100 µg Human/Canine Goat IgG, AF1699-SP 25 µg Human/Equine Mouse IgG 2B, ICC/IF MAB16991 100 µg MAB16991-SP 25 µg, ICC/IF, IHC, IP BAM1124 250 µg FAB1124A-025 25 Tests FAB1124A-100 FAB1124F-025 25 Tests FAB1124F-100 FAB1124P-025 25 Tests FAB1124P-100 MAB1124-100 100 µg, ICC/IF, IHC, IP MAB1124-500 500 µg MAB1124-SP 25 µg CD MLDP5 1 Vial FAB1124C-025 25 Tests FAB1124C-100 FAB1124N-025 25 Tests FAB1124N-100 Mouse NEW Rat IgG 2B FAB1124V-025 25 Tests Mouse NEW Rat IgG 2B FAB1124R-025 25 Tests Mouse NEW Rat IgG 2B FAB1124T-025 25 Tests Mouse NEW Rat IgG 2B FAB1124S-025 25 Tests
Antibody Species Source Application(s) Catalog # Size AF1219 100 µg Human Goat IgG, ICC/IF, AF1219-SP 25 µg FAB1219P MAB1219 500 µg, ICC/IF Integrin av/cd51 MAB1219-SP 25 µg, ICC/IF MAB12191 100 µg MAB12191-SP 25 µg Human Goat IgG, ICC/IF, BAF1219 50 µg FAB1219G-025 25 Tests Human Goat IgG, BAF1778 50 µg FAB17781P, ICC/IF, IHC, IP, MAB1778 100 µg MAB1778-SP 25 µg B/N,, ICC/IF, IP MAB17781 100 µg MAB17781-SP 25 µg, FA, ICC/IF, IP MAB17782 100 µg MAB17782-SP 25 µg FAB17783A FAB17783P AF1778 100 µg Human/Canine Goat IgG, ICC/IF, AF1778-SP 25 µg Integrin b1/cd29 Human/Porcine/Equine Mouse IgG 2B, ICC/IF, MAB17783 100 µg MAB17783-SP 25 µg AF2405 100 µg Mouse Goat IgG, IHC, AF2405-SP 25 µg Mouse Goat IgG BAF2405 50 µg, ICC/IF, IHC, MAB2405 100 µg MAB2405-SP 25 µg FAB2405A FAB2405F FAB2405P-025 25 Tests FAB2405P-100 FAB2405C AF1730 100 µg Human Goat IgG B/N,, ICC/IF, AF1730-SP 25 µg Human Goat IgG, BAF1730 50 µg FAB1730F FAB1730P, ICC/IF, MAB1730 500 µg MAB1730-SP 25 µg Integrin b2/cd18 FAB1730A MAB2618 100 µg MAB2618-SP 25 µg Mouse Goat IgG FAB2618F Mouse Goat IgG FAB2618P AF2618 100 µg Mouse/Canine Goat IgG, FA, AF2618-SP 25 µg Learn more rndsystems.com/integrins
Antibodies continued Antibody Species Source Application(s) Catalog # Size AF2266 100 µg Human Goat IgG, AF2266-SP 25 µg Human Goat IgG, BAF2266 50 µg FAB2266A Integrin b3/cd61 FAB2266P, MAB2266 100 µg MAB2266-SP 25 µg Mouse NEW Rat IgG 1 FAB8557P-025 25 Tests Mouse NEW Rat IgG 1 FAB8557A-025 25 Tests Mouse NEW Rat IgG 1 MAB41182 100 µg AF4060 100 µg Human Sheep IgG, ICC/IF, AF4060-SP 25 µg, ICC/IF, MAB4060 100 µg MAB4060-SP 25 µg FAB4060A FAB4060G Integrin b4/cd104 MAB4054 100 µg, IHC, MAB4054-SP 25 µg AF4054 100 µg Mouse Goat IgG AF4054-SP 25 µg FAB4054F FAB4054P Mouse Goat IgG BAF4054 50 µg Human Sheep IgG, ICC/IF, BAF3824 50 µg AF3824 100 µg Human Sheep IgG, ICC/IF, Integrin b5 AF3824-SP 25 µg AF8035 100 µg Mouse/Rat Sheep IgG, IHC, AF8035-SP 25 µg AF4155 100 µg Human Sheep IgG, ICC/IF, AF4155-SP 25 µg Human Sheep IgG, ICC/IF, BAF4155 50 µg, ICC/IF MAB4155 100 µg MAB4155-SP 25 µg FAB4155A Integrin b6 FAB4155P MAB41551 100 µg MAB41551-SP 25 µg AF2389 100 µg Mouse Goat IgG ICC/IF, AF2389-SP 25 µg Mouse Goat IgG ICC/IF, BAF2389 50 µg ICC/IF, MAB2389 100 µg MAB2389-SP 25 µg
Antibody Species Source Application(s) Catalog # Size AF4669 100 µg Human Sheep IgG, AF4669-SP 25 µg, ICC/IF MAB4669 100 µg MAB4669-SP 25 µg Human Sheep IgG BAF4669 50 µg FAB4669F FAB4669P Integrin b7 AF3060 100 µg Mouse Goat IgG, AF3060-SP 25 µg MAB3060 100 µg MAB3060-SP 25 µg FA MAB30601 100 µg MAB30601-SP 25 µg MAB30602 100 µg MAB30602-SP 25 µg MAB4775 100 µg Integrin b8 MAB4775-SP 25 µg FAB4775A Antibody Application Key: B/N - Blocking/Neutralize CD - Cell Depletion - Flow Cytometry FA - Functional Assay ICC/IF - Immunofluorescence IHC - Immunohistochemistry IP - Immunoprecipitation - Western Blot Learn more rndsystems.com/integrins
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