Impact of NM23-M1 "knock-out" on metastatic potential of hepatocellular carcinoma: a transgenic approach

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Impact of NM23-M1 "knock-out" on metastatic potential of hepatocellular carcinoma: a transgenic approach

NM23-M1 "knock- out" mice (without NDPK A) Experimental models of hepatocarcinogenesis Chemical model (Diethylnitrosamine, DEN) : single IP injection in 15-days-old mice (10 g/g body weight). Double transgenic mice : ASV/NM23-M1 / breeding of NM23-M1 "knock-out" mice with ASV mice expressing specifically in the liver the SV40 large T antigen under the control of antithrombin III promoter and developping hepatocellular carcinoma (P. Briand).

Primary tumor development in DEN-treated and ASV mice No difference in tumor primary formation between NM23-M1 +/+ and NM23-M1 -/- mice.

Immunohistochemical analysis with Hep-Par 1 antibody Only the lung nodules of ASV mice are hepatocarcinoma metastases.

Metastatic dissemination in ASV mice Highly significant increase in the metastatic incidence in ASV/NM23-M1 -/- mice.

NM23 silencing and consequences in epithelial cancer cell models

NM23-H1 silencing and cell scattering (1) HepG2 cells

NM23-H1 silencing and cell scattering (2) C Si 24h 48h 72h 24h 48h 72h NM23-H1 silenced cells show a random spatial distribution

NM23-H1 silencing and cell scattering (3) PLC/PRF/5 cells HCT8/S11 cells NM23-H1 silenced cells are scattered and show cellular extensions

NM23-H1 silencing and intercellular adhesion HepG2 cells fast aggregation assay slow aggregation assay

NM23-H1 silencing and intercellular adhesion HCT8/S11 cells fast aggregation assay slow aggregation assay Inhibition of intercellular adhesion by NM23-H1 silencing

NM23-H1 silencing and adherens junctions NM23-H1 silencing induces the disruption of adherens junction complexes

NM23-H1 silencing and adherens junctions NM23-H1 silencing induces -catenin-tcf/lef-1 transactivation through a GSK-3 -independent mechanism

NM23-H1 silencing and cell motility HepG2 cells NM23-H1 silenced cells exhibit higher motility

NM23-H1 silencing and cell motility HCT8/S11 cells NM23-H1 silencing induces directional migration

NM23-H1 silencing and actin cytoskeleton Fluorescence microscopy Scanning electron microscopy NM23-H1 silencing induces filopodia-like structures and Rac1 activation

NM23-H1 silencing and invasion Matrigel Native collagen I -NM23-H1 silencing induces invasion in matrigel and collagen I. -Conditioned media from control and silenced cells does not promote invasion.

NM23-H2 silencing and invasion Native collagen I NM23-H2 silencing does not induce invasion in collagen I.

NM23 silencing, invasion and NDPK activity H1: invasion H1 H2 H2: no invasion H1 H2 20% 15% 52% 38% The NDPK intrinsic activity of NM23-H1 only represents a minor fraction of the global cellular NDPK activity

NM23-H1 silencing, invasion and signaling HepG2 cells HCT8/S11 cells Involvement of MAPK, PI3K, Rho/ROCK, and Src in invasion induced by NM23-H1 silencing

NM23-H1 silencing, invasion and signaling Hyperactivation of MAPK and PI3K/Akt/S6K induced by NM23-H1 silencing

NM23-H1 silencing and invadopodia (1)

NM23-H1 silencing and invadopodia (2) Depletion of NM23-H1 stimulates matrix degradation, invadopodia formation and invasion in a MT1-MMPdependent manner

NM23-H1 silencing and invasion in other studies Invasion induced by NM23-H1 silencing -in melanoma cell lines (Bakalian et al, 2007) -in lung cancer cell lines (Ma et al, 2008) -in trophoblast cells (Xie et al, 2010) Microarray analysis: MMP-1 and MMP-2 upregulated genes, E-cadherin downregulated gene (Ma et al, 2008) NM23-H1 silencing induced increased levels of activated FGF receptor (Hsu et al, 2006)

NM23-H1 silencing and proliferation Flow cytometry Soft agar assay No effect on anchorage -dependent and -independent growth

NM23-H1 in hepatocellular carcinoma (1)

NM23-H1 in hepatocellular carcinoma (2)

NM23-H1 in colon carcinoma (1)

NM23-H1 in colon carcinoma (2) Loss or reduction of NM23-H1 at the invasive front of clinical tumors in comparison to the central body of the tumor

Phénotype épithélial (NM23-H1 >0) E-cadhérine -caténine Cohésion et polarité épithéliale NM23-H1 Déstabilisation des jonctions adhérentes (Ecadhérine). Translocation nucléaire ( -caténine) Phénotype mésenchymateux (NM23-H1 <0) Dispersion cellulaire MEC TEM partielle Motilité INVASION Collagène type I Matrigel Gélatine Invadopodes (MT1-MMP) Perte de NM23-H1 au front invasif de tumeurs primaires Src Rac1-GTP MEK PI3K p38 ERK Akt LEF-TCF (Wnt) MT1-MMP Tiam-1 JNK -caténine

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