Assays Patents: EP 2362222, AU 2011217190 New Combinations New Possibilities
Technology Classical Handling of Autoimmune Diagnostics 2-Step Diagnostics 1 st Screening 2 nd Confirmation Cell based IFA ELISA / Dot 1-Step Diagnostics Next Generation IFA ANA / ANA 2 ANCA / RPGN CeliAK - Technology Suitable for all manual fluorescence microscopes!
Technology Screening and Confirmation at once! One Well - One Step - One Assay Example CytoBead ANCA 148077020001 ANCA GBM negative Reference bead Granulocytes (EtOH) canca pattern PR3 positive MPO negative Reference bead + + Antigen-coated bead (e.g. PR3) Primary antibody (patient sera) Secondary antibody fluorochrome labeled CytoBead is time saving and economical.
Bead evaluation Bead Compartment Markers for better Orientation 4 1 Cells 2 3 Cells 1 2 Reading direction Compartment 1 to cells to compartment 2 Compartment marker Reading direction Cells to compartment 1 to 2 to 3 to 4 Compartment marker Assays: ANCA, RPGN, CeliAK Assays: ANA, ANA 2 Evaluation of Fluorescence Intensity (semi-quantitative) +++ ++ + +/- +++ ++ + +/- Enlarged images (10x objective, obtained with AKLIDES, MEDIPAN GMBH)
ANA / ANA 2 Fast and easy ANA Diagnostics ANA (Anti-Nuclear Antibodies) are autoantibodies (AAB) which recognize conserved nuclear antigens. ANA show a characteristic staining of nuclear structures with indirect immunofluorescence on human epithelial cells (HEp-2). The confirmation of ANA is done in accordance to the target antigens. Unique Combination of HEp-2 Cells with antigen coated Microbeads 148081020001 ANA CytoBead ANA 4 HEp-2 cells Advantages of the CytoBead ANA / ANA 2 Screening with standardized HEp-2 cells Confirmation of 8 ANA specificities with microbeads 3 1 2 CytoBead ANA / ANA 2 HEp-2 cell pattern Compartment Bead (positive) Possible beadfluoresce nce Possible antigens Clinical relevance dsdna dsdna Systemic lupus erythematosus (SLE) Homogenous 3 Scl-70 Scl-70 Marker for progressive systemic sclerosis (PSS) 2 Sm RNP-Sm Sm nrnp Sm antibodies highly specific for SLE; high anti-nrnp titers specific for mixed connective tissue disease (MCTD) together with other ANAs in rheumatoid arthritis (RA), SLE, PSS Speckled Ro60 Ro60/SS-A 4 Ro52 Ro52/SS-A Often in primary Sjögren s syndrome, anti-ss-a often in neonatal lupus 1 La La/SS-B Centromere 1 CENP-B Centromeric proteins of the chromosomes Marker for CREST syndrome, rarely in diffuse scleroderma and Raynaud s phenomenon CytoBead ANA 2, Jo-1 microbead replaces CENP-B microbead Cytoplasmic 1 Jo-1 Jo-1 (PL-7, PL-12) Polymyositis, dermatomyositis Small bead: negative Large bead: negative Reference bead in each compartment
ANCA / RPGN Fast and easy ANCA Diagnostics 148077020001 ANCA CytoBead ANCA Granulocytes (EtOH) 1 2 ANCAs (Anti-Neutrophil Cytoplasmic Antibodies) play an important role in the diagnosis of ANCA associated vasculitides (AAV). According to international guidelines ANCA screening is performed using indirect immunofluorescence (IIF) with ethanol fixed granulocytes, whereby cytoplasmic (canca; antigen PR3) and perinuclear (panca; antigen MPO) IIF patterns can be differentiated. Unique Combination of Ethanol fixed Granulocytes with antigen coated Microbeads Advantages of the CytoBead ANCA Screening with standardized ethanol fixed granulocytes Confirmation of 3 antibody specificities with microbeads CytoBead RPGN Granulocytes (EtOH) 1 2 RPGN (Rapid Progressive Glomerulo-Nephritis) Especially for Use in Emergency Situations Advantages of the CytoBead RPGN Screening with standardized ethanol fixed granulocytes Confirmation of 4 antibody specificities with microbeads CytoBead ANCA / RPGN Cell pattern Compartment Bead (positive) Possible beadfluorescen ce Possible antigens Clinical relevance negative 1 GBM GBM Goodpasture syndrome cytoplasmic 2 PR3 PR3 Granulomatosis with polyangiitis perinuclear 2 MPO MPO Microscopic polyangiitis, eosinophilic granulomatosis with polyangiitis, polyateritis nodosa Additionally in CytoBead RPGN nuclear 1 dsdna dsdna Systemic lupus erythematosus, pathogenesis of lupus nephritis Small bead: negative Large bead: negative Reference bead in each compartment
CeliAK Fast and easy Diagnostics of Celiac Disease or Dermatitis Herpetiformis and Detection of IgA Deficiency Celiac disease (gluten induced enteropathy) is an intolerance to gluten. This intolerance leads to extended lesions of the mucous membranes, which manifests as a flat mucosa. Gliadin, the alcohol-soluble fraction of gluten, is responsible for the emergence of celiac disease. Gliadin induces inflammatory processes in the small intestinal mucosa as part of the humoral and cellular immune processes. The diagnosis of celiac disease is characterized through highly specific AAB against transglutaminase 2 (tissue transglutaminase, ttg) and deamidated gliadin (DG). Endomysial antibodies (EmA) are directed against extracellular ttg. Celiac antibodies are typically of IgA class. In celiac patients with IgA deficiency, antibodies of the IgG type form the basis of diagnostics. 148080020001 CeliAK CytoBead CeliAK Monkey oesophagus 1 2 Unique Combination of esophageal Tissue with antigen coated Microbeads Advantages of the CytoBead CeliAK Screening with standardized monkey esophageal tissue Confirmation of 3 antibody specificities with microbeads CytoBead CeliAK Pattern Tissue Compartment Bead (positive) Possible beadfluorescen ce Possible antigens Clinical relevance honeycomb pattern of muscularis mucosa 1 ttg ttg 1 DG DG Celiac disease Dermatitis herpetiformis 2 IgA IgA IgA deficiency Small bead: negative Large bead: negative Reference bead in each compartment
Order information CytoBead ANA Order no. 8065 CytoBead ANA 2 Order no. 8220 CytoBead ANCA Order no. 8063 CytoBead RPGN Order no. 8066 CytoBead CeliAK Order no. 8064 Literature Simultaneous detection of celiac disease-specific IgA antibodies and total IgA. Grossmann K, Röber N, Hiemann R, Rödiger S, Schierack P, Reinhold D, Laass M.-W., Conrad K, Roggenbuck, D. Auto Immun Highlights. 2016 Dec, 7(1): 2. Second generation analysis of antinuclear antibody (ANA) by combination of screening and confirmatory testing. Scholz J, Grossmann K, Knütter I, Hiemann R, Sowa M, Röber N, Rödiger S, Schierack P, Reinhold D, Bogdanos DP, Meroni PL, Radice A, Conrad K, Roggenbuck D. Clin Chem Lab Med 2015, 53(12): 1991-2002. Simultaneous automated screening and confirmatory testing for vasculitis-specific ANCA. Sowa M, Grossmann K, Knütter I, Hiemann R, Röber N, Anderer U, Csernok E, Bogdanos DP, Borghi MO, Meroni PL, Schierack P, Reinhold D, Conrad K, Roggenbuck D. PLoS One. 2014, 9(9):e107743. The CytoBead assay - a novel approach of multiparametric autoantibody analysis in the diagnostics of systemic autoimmune diseases. Sowa M, Grossmann K, Scholz J, Röber N, Rödiger S, Schierack P, Conrad K, Roggenbuck D, Hiemann R. J Med Lab 2014; 38:309-17. GA Generic Assays GmbH Ludwig-Erhard-Ring 3 15827 Dahlewitz/Berlin Germany Phone: +49 33708-9286-0 Fax: +49 33708-9286-50 info@genericassays.com GA-BR-E-CytoBead_v003-2016-03-17