177189 1388 1 11 (Fusarium oxysporum f. sp. radiciscucumerinum) **** *** ** * 88/2/22 : 86/12/1 : (Cucumis sativus) Fusarium oxysporum f. sp. radiciscucumerinum 16. (Cucurbitaceae) (Cucumis melo). F. oxysporum f. sp. radiciscucumerinum 20. SR3645664 PSR3645007 Khassib Jakie Festival FDC101 Ayat 120118 PSR36 Nicoo 100 Negeen Nasco Janette CB61688222 8Ayat 32PV Storm. Rubahs Rubahl 47112 Fusarium oxysporum f. sp. : radiciscucumerinum (emolavi@gmail.comemail: ) * ** *** **** ١٧٧
40 20..(11).(10). 18 Dominica Amazing Sienna Korinda. Aviance Euphoria.(9) Tkw152, Tkw145, Tkw1 Festival 87, Tkw1127, Tkw1228, Astoria Number one Rubahs Columvia Cashmere Parma RubahL, PSR3645007, Evergreen PSR03645009, Tkw29, PSR3647112, PSR3645664, TKW197, TKW196, TKW180, TKW168, PSR3644326, PX3646330, Bs03641491, Tkw1110.(10) F. oxysporum Schlechtend.:Fr. f. sp. ) radiciscucumerinum f. sp. nov. 198990 (Vakalounakis..(11) 1998 1994 2000 1999.(13 8 6 3) 138182 2060.(10)..... 178
2 1).(7.. 100 10 90) 10 (. 121 20. 25. ) (1:2:1:1.. 11 J 1, P 1, P 7, P 8, Ps, H 1, H 2.. 10 1 4/5 ) 25. ( 25.. 2 3. 1 APDA (Acidified Potato Dextrose Agar) 2 PSA (Potato Sucrose Agar) 3 CLA (Carnation Leaf Agar) 179
. ( P 1 J 1 2830 1517. ) 16 3. (. F. oxysporum f. sp. radiciscucumerinum F. oxysporum f. sp. cucumerinum 1 PI390265 Ashley. 20.(1 ) P 1 J 1.. 1 Plant Protection Institute, Heraklio crete Greece, 2006..(10) 1517 2830. :1 : ) :2 ( :3.(13). SAS 9.0. ) 180
. 4/8 9/7 2/4 3/6 1 1 ).(A 25 37/5 3/85.(B 1 ) 10.(C 1 ) F. oxysporum Schlechtend.:Fr.(7 2 1) 15 P 1 J 1..(2 ). 2830 1517. 10. ( ) 30 ) 20 3. (. SAS 9.0. ) (. APDA 1 False head 181
1 Table 1 Different cultivars of greenhousecucumber used for study of resistance No. Cultivar No. Cultivar 1 Ayat 11 Nicoo100 2 8Ayat 12 PSR3647112 3 CB61688222 13 PSR3645007 4 FDC101 14 RubahL 5 Festival 15 RubahS 6 Jakie 16 7 Janette 17 SR3645664 8 Khassib 18 Storm 9 Nasco 19 120118 10 Negeen 20 32PV. Peto & Seed The hybrid cultivars were produced by Peto and Seed American company and were provided from Varamin agricultural research center. A B C 25 C CLA F. oxysporum f. sp. radiciscucumerinum 1 570. :C :B :A Fig. 1. Microscopic view of fungal organs of F. oxysporum f. sp. radiciscucumerinum, were observed seven days after culturing on CLA (at 25 C in dark), A: false heads and microconidia, B: Pathogen microconidia and macroconidia, C: Pathogen chlamydospores, 570 182
F. oxysporum f. sp. radiciscucumerinum 2 Table 2 The average of disease severity caused by different isolates of F. oxysporum f. sp. radiciscucumerinum. Isolates of F. oxysporum f. sp. radiciscucumerinum Origin of isolates Cucumber cultivars Disease severity score (03) J1 Jiroft Negin 2.3 ± 0.2 a P1 Pishva 1.8 ± 0.2 b H2 Habibabad 1.3 ± 0.2 c P8 Pishva 1 cd Ps Pishva 0.8 ± 0.2 d P7 Pishva 0.3 ± 0.2 e H1 Habibabad 0 e Control 0 e :1 :0. 03 :2 ±. :3.(P 0/01) Disease severity was assessed with a 0 3 rating visual scale where zero, no symptoms; 1: light or moderate rot of tap root, secondary roots and crown, light vascular discoloration in the stem; 2: severe rot of tap root, secondary roots and crown, with or without wilting and stunting, vascular discoloration in the stem and 3: dead seedlings. Data are means of three replicate ± SE. Significant differences are denoted by different letter according to Duncan's Multiple Range Test (P 0.01). F. oxysporum f. sp. cucumerinum.(11) PI390265 Ashley Ashley PI390265 30 P 1 J 1.(3 ) F. oxysporum f. sp. radiciscucumerinum 183
.(4 ) cucumerinum F. oxysporum f. sp. radicis F. oxysporum f. sp. radiciscucumerinum 3 Table 3 Determination of host range of two isolates of F. oxysporum f. sp. radiciscucumerinum Plant species P 1 Inoculated with P 1 isolate J 1 Inoculated with J 1 isolate Plant species P 1 Inoculated with P 1 isolate Inoculated with J 1 isolate J 1 Zea mays Lactuca sativa Tritichum aestivum Cucumis sativus + + Cicer arietinum Cucumis melo + + (Cantaloupe) Pisum sativum Cucumis melo + + (Melon) Phaseolus vulgaris Cucurbita maxima Betta vulgaris Cucurbita pepo Lycopersicon esculentum Cucurbita mixta Solanum melongena Citrullus vulgaris 4 Table 4 The reaction of two differential cucumber cultivars to two form specialis of pathogen. F. oxysporum f. sp. radiciscucumerinum F. oxysporum f. sp. cucumerinum Differential cucumber J1 P1 III II I cultivars R R W Ashley H H H PI390265 : H (Healthy) : R (Rot) : W (Wilt) W (Wilt): Wilting with stem and root vessles discoloration, R (Rot): Corrtical rot of stem and root, H (Healthy): Healthy, without wilting and rot symptoms of stem and root 184
Jakie Festival Ayat 120118 SR36 PSR3645007 Khassib FDC101 32 8Ayat Storm 45664 Negeen Nasco Janette CB61688222 PV Rubahs Rubahl,PSR36471121 Nicoo100.(3 2 1 ) 30 10.. 3 2.5 P1 isolate J1 isolate Disease severity (03) 2 1.5 1 0.5 0 Festival Nasco Rubahs Rubahl Khassib SR3645664 Nicoo100 Janette psr3647112 psr3645007 Cultivars of cucumber 8Ayat Jakie 32Pv FD101 Negeen Storm CB6168 12018 Ayat F. oxysporum f. sp. radicis J 1 P 1 1 30 cucumerinum Fig. 1. Comparison of disease severity caused by P 1, J 1 isolates of F. oxysporum f. sp. radiciscucumerinum on various cultivars of cucumber 30 days after inoculation under glasshouse conditions 185
F. oxysporum f. sp. 2 30 radiciscucumerinum Fig. 2. Comparison the means of fresh weight of root of various cucumber cultivars caused by two isolates of F. oxysporum f. sp. radiciscucumerinum in proportion to control plants, 30 days after inoculation under glasshouse conditions fresh weight of shoot (gr) ( ) fresh weight of root (gr) ( ) Cultivars of cucumber Cultivars of cucumber F. oxysporum f. 3 30 sp. radiciscucumerinum Fig. 3. Comparison the means of fresh weight of shoot of various cucumber cultivars caused by two isolates of F. oxysporum f. sp. radiciscucumerinum in proportion to control plants, 30 days after inoculation under glasshouse conditions 186
390265..(11) F. solani f. sp. cucurbitae F. oxysporum f. sp. radicis cucumerinum F. oxysporum. F. solani F. oxysporum f. sp.. radiciscucumerinum.(11 7 1) 16 ) (.(11).(10) F. oxysporum f. sp. luffae 11.(11 10) F. oxysporum f. sp. radiciscucumerinum.... ( 1729) F. oxysporum f. sp. cucumerinum 29 187
Dominica Amazing Sienna Korinda Aviance Euphoria.(9) Tkw152, 28 Tkw145, Tkw187, Tkw1127, Tkw1228, Astoria, Number one, Rubahs, Columvia,, Festival PSR3645007, PSR03645009, Tkw29, RubahL, Evergreen, Cashmere, Parma, PSR3647112, PSR3645664, TKW197, TKW196, TKW180, TKW168, PSR36 44326, PX3646330, Bs03641491, tkw1.(10) 110 ( ).... F. luffae oxysporum f. sp..(11) F. oxysporum f. sp. F. oxysporum melonis f. sp. melonis.(13) (F. oxysporum f. sp. cucumerinum).(14 11 5 4). Festival FDC101 Ayat 120118 SR3645664 PSR3645007 Khassib Jakie Storm ( ) 8Ayat 32PV Negeen Nasco Janette CB61688222 Rubahs Rubahl PSR3647112 Nicoo100 ) (. 18 188
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Journal of Agriculture, Vol. 11, No. 1, Spring 2009 Investigation the resistance of greenhousecucumber cultivars to Fusarium stem and root rot disease (Fusarium oxysporum f. sp. radiciscucumerinum) E. Molavi *, H. Aminian **, H. R. Etebarian *** and D. Shahriari **** Abstract For determination the host range of Fusarium stem and root rot of greenhouse cucumber (Cucumis sativus) that caused by Fusarium oxysporum f. sp. radiciscucumerinum, after isolation, identification and study of pathogenisity, 16 species of several plant families were artificially inoculated with this pathogen by sand and corn meal contaminated with pathogen, two varieties of Cucumis melo, of Cucurbitaceous developed stem and root rot symptoms similar to those that were developed on cucumber. Symptomology and host range studies indicate that this pathogen is F. oxysporum f. sp. radiciscucumerinum. Reactions of 20 cucumber cultivars were tested, ranged from susceptible to tolerant to this disease under glasshouse conditions showed that the tolerant cultivars were 120118, Ayat, FDC101, Festival, Jakie, Khassib, PSR3645007, SR3645664 and Storm and the most susceptible cultivars were 32PV, 8Ayat, CB61688222, Janette, Nasco, Negeen, Nicoo 100, PSR3647112, Rubahl, Rubahs and. Keywords: Cucumber, Fusarium rot, Fusarium oxysporum f. sp. radiciscucumerinum, Susceptibility, Tolerant cultivars * Former M.Sc. Student, Department of Entomology and Plant pathology, College of Abouraihan, University of Tehran, Tehran Iran (Email: emolavi@gmail.com) ** Assistant Professor, Department of Entomology and Plant pathology, College of Abouraihan, University of Tehran, Tehran Iran *** Professor, Department of Entomology and Plant pathology, College of Abouraihan, University of Tehran, Tehran Iran **** Academic press, Department of Plant Pathology, Agricultural Research Center of Varamin, Tehran Iran