Core-Shell Technology for Proteins and Peptides

Core-Shell Technology for Proteins and Peptides Better BioSeparations on HPLC and UHPLC Systems www.phenomenex.com/aeris

Aeris Core-Shell Technology Core-Shell Particles Precision Engineered for Protein and Peptide Separations Core-shell particle technology provides striking increases in peak capacity and resolution at lower backpressures, giving chromatographers the ability to achieve ultra-high performance on ANY system, HPLC or UHPLC. Optimizing the pore size and shell thickness for intact proteins or smaller peptide fragments provides well-defined depth penetration of biomolecules leading to maximum separation power. 3.6 µm Core-Shell Particle 2.6 µm Solid Core.5 µm Porous Shell 1.7 µm Core-Shell Particle 1.25 µm Solid Core.22 µm Porous Shell Aeris Peptide 3.6 µm 1.7 µm Aeris WIDEPORE 3.2 µm Solid Core 3.6 µm Core-Shell Particle.2 µm Porous Shell 3.6 µm Aeris TM WIDEPORE and make a perfect pair for peptide mapping. HPLC SYSTEM 3.6---3.2 solid core 3.6---2.6 solid core 1.7---1.25 solid core 3.6 µm PROTEIN OR PEPTIDE < 1 kda UHPLC SYSTEM 1.7 µm 3.6 µm Hydrophilic Proteins 3.6 µm > 1 kda HPLC OR UHPLC SYSTEMS Hydrophobic Proteins 3.6 µm XB-C8 Very Hydrophobic Proteins 3.6 µm 213 Phenomenex, Inc. All rights reserved.

Aeris Peptide Ultra-High Resolving Power on HPLC and UHPLC Systems with 3.6 µm Columns The Aeris PEPTIDE 3.6 µm core-shell column was designed with one purpose in d: to maximize the separation of large numbers of peptides on any HPLC or UHPLC system. Because core-shell particles remove the backpressure constraints of HPLC or UHPLC systems, chromatographers can achieve the ultra-high performance of similar length sub-2 µm columns at a fraction of the backpressure. 8 3.6 µm 125 bar Resolves an Additional Pair of Peptides 8 *Waters ACQUITY BEH 1.7 µm C18 465 bar Conditions for both columns: Column: 3.6 µm ACQUITY BEH 1.7 µm C18 Dimensions: 15 x 2.1 mm Mobile Phase: A: Water with.1 % TFA B: Acetonitrile with.1 % TFA Gradient: A/B (97:3) for 5 to A/B (55:45) over 55 Flow Rate:.3 ml/ Temperature: 4 ºC Instrument: Agilent 12SL Detection: UV @ 214 nm (ambient) Sample: Human IgG Tryptic Digest 6 6 4 4 2 App ID 19928 2 App ID 1993 25 3 35 4 25 3 35 4 Maximize Performance on UHPLC Systems with 1.7 µm Technology AU With pressure stability up to 1, bar and the high efficiencies brought about by core-shell particle technology, the sub-2 µm column produces breakthrough chromatographic performance on UHPLC systems. Use 1.7 µm columns to boost the performance of sub-2 µm fully porous peptide mapping methods. 1.7 µm AU *Waters ACQUITY BEH 1.7 µm C18 Conditions for both columns: Column: 1.7 µm ACQUITY BEH 1.7 µm C18 Dimensions: 15 x 2.1 mm Mobile Phase: A: Water with.1 % TFA B: Acetonitrile with.8 % TFA Gradient: A/B (97:3) for 1.5 to A/B (6:4) over 11 to A/B (5:95) over 1 Flow Rate:.5 ml/ Temperature: 4 ºC Injection Volume: 5 µl Instrument: Aglient 12SL Detection: UV @ 214 nm (ambient) Sample: Alpha-Casein Tryptic Digest.3.3 Identify More Peaks Peak count = 79 Peak count = 57.2.2.1.1 App ID 19897 App ID 19895 2 4 6 8 1 12 2 4 6 8 1 12 * Waters and ACQUITY are registered trademarks, and BEH Technology is a trademark of Waters Corporation. Phenomenex is not affiliated with Waters Corporation. Study was performed using new columns and, to the extent possible, identical experimental conditions were applied. Comparative separations may not be representative of all applications.

Aeris Widepore 2 15 5 32 34 36 38 4 42 2 15 5 32 34 36 38 4 42 2 15 5 32 34 36 38 4 42 Utilize Long Columns to Maximize Resolution on HPLC and UHPLC Systems Aeris WIDEPORE 3.6 µm Increased Resolution Using Core-Shell Technology *Waters ACQUITY BEH3 1.7 µm C18 12 bar 4 bar App ID 19822 App ID 19823 15 x 2.1 mm 15 x 2.1 mm Moving to a Longer Column 25 x 2.1 mm 3.6 µm 16 bar Even Greater Resolution on Your UHPLC System App ID 19824 Conditions for all columns: Column: 3.6 µm ACQUITY BEH3 1.7 µm C18 Dimensions: as noted in chromatogram Mobile Phase: A: Water with.1 % TFA B: Acetonitrile Gradient: A/B (9:1) for 5 to A/B (5:5) over 45 Flow Rate:.2 ml/ Temperature: 22 ºC Injection Volume: 2 µl Instrument: Agilent 12SL Detection: UV @ 21 nm Sample: Degraded Myoglobin Easy Method Development with Three Selectivities 3.6 µm Core-Shell Stationary Si Si XB- C18 XB- C8 Maximum hydrophobicity recommended for: Proteins Hydrophilic proteins PEGylated proteins High temperature separations Alternative selectivity for peptide mapping XB-C8 Moderate hydrophobicity recommended for: Large proteins Moderately hydrophobic proteins Monoclonal antibodies Glycosylated proteins High temperature separations Low hydrophobicity recommended for: Very large proteins Very hydrophobic proteins Membrane proteins Least retentive * Waters and ACQUITY are registered trademarks, and BEH Technology is a trademark of Waters Corporation. Phenomenex is not affiliated with Waters Corporation. Study was performed using new columns and, to the extent possible, identical experimental conditions were applied. Comparative separations may not be representative of all applications.

Ordering Information Material Characteristics Packing Material Total Particle Porous Size (µm) Shell (µm) Core Size (µm) Pore Size (Å) ph Stability Temp Stability Pressure Stability 3.6.2 3.2 2 1.5-9 9 C 6 bar 1.7.22 1.25 1.5-9 9 C bar Order Online for Exclusive Savings! 3.6.5 2.6 1.5-9 9 C 6 bar www.phenomenex.com/aeris 5 x 2.1 x 2.1 15 x 2.1 25 x 2.1 B-4482-AN D-4482-AN F-4482-AN G-4482-AN AJ-8783 XB-C8 B-4481-AN D-4481-AN F-4481-AN G-4481-AN AJ-8785 B-4486-AN D-4486-AN F-4486-AN G-4486-AN AJ-8899 3.6 µm Minibore Columns (mm) x 4.6 15 x 4.6 25 x 4.6 D-4482-E F-4482-E G-4482-E AJ-8769 3.6 µm Analytical Columns (mm) UHPLC Column Protection Cartridge Holder Cartridge with Holder XB-C8 D-4481-E F-4481-E G-4481-E AJ-8771 D-4486-E F-4486-E G-4486-E AJ-891 5 x 2.1 x 2.1 15 x 2.1 B-456-AN D-456-AN F-456-AN AJ-8948 1.7 µm Minibore Columns (mm) 5 x 2.1 x 2.1 15 x 2.1 25 x 2.1 B-457-AN D-457-AN F-457-AN G-457-AN AJ-8948 3.6 µm Minibore Columns (mm) x 4.6 15 x 4.6 25 x 4.6 D-457-E F-457-E G-457-E AJ-8946 ea AJ-9 Price 3.6 µm Analytical Columns (mm) If you are not completely satisfied with your Aeris core-shell columns, return the column with comparative data within 45 days for a FULL REFUND. ULTRA Cartridge Holder* (for 2.1 to 4.6 mm ID columns) ULTRA Guard Cartridge Holder * ULTRA cartridges require holder part number AJO-9.

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