CELL CULTURE MEDIA AND REAGENTS

CELL CULTURE MEDIA AND REAGENTS Classical Media Buffered Salt Solutions Reagents & Supplements

CELL CULTURE MEDIA AND REAGENTS MEDIA DULBECCO S MODIFIED EAGLE S MEDIUM (DMEM) A modification of Basal Medium Eagle (BME), Dulbecco s Modified Eagle's Medium (DMEM), that contains approximately four times as much vitamins and amino acids and two to four times as much glucose as the original formulation. DMEM is suitable for most types of cells, including human, monkey, hamster, rat, and mouse. DMEM is available in a variety of formulations including high or low glucose, with or without sodium pyruvate, and with or without L-glutamine. Description DMEM 4.5 g/l w/ Sodium Pyruvate and DMEM 4.5 g/l w/ Sodium Pyruvate, w/out DMEM 4.5 g/l w/out Sodium Pyruvate, w/ DMEM 4.5 g/l w/out Sodium Pyruvate and DMEM 1.0 g/l w/ Sodium Pyruvate and DMEM 1.0 g/l w/ Sodium Pyruvate, w/out Cat. No. VWRL0101-0500 VWRL0148-0500 VWRL0100-0500 VWRL0102-0500 VWRL0111-0500 VWRL0149-0500 Components mg/l mg/l mg/l mg/l mg/l mg/l Glycine 30.00 30.00 30.00 30.00 30.00 30.00 L-Arginine HCl 84.00 84.00 84.00 84.00 84.00 84.00 L-Cystine 2HCl 63.00 63.00 63.00 63.00 63.00 63.00 584.00 0.00 584.00 0.00 584.00 0.00 L-Histidine HCl H 2 O 42.00 42.00 42.00 42.00 42.00 42.00 L-Isoleucine 105.00 105.00 105.00 105.00 105.00 105.00 AMINO ACIDS INORGANIC SALTS VITAMINS OTHER L-Leucine 105.00 105.00 105.00 105.00 105.00 105.00 L-Lysine HCl 146.00 146.00 146.00 146.00 146.00 146.00 L-Methionine 30.00 30.00 30.00 30.00 30.00 30.00 L-Phenylalanine 66.00 66.00 66.00 66.00 66.00 66.00 L-Serine 42.00 42.00 42.00 42.00 42.00 42.00 L-Threonine 95.00 95.00 95.00 95.00 95.00 95.00 L-Tryptophan 16.00 16.00 16.00 16.00 16.00 16.00 L-Tyrosine 2Na 2H 2 O 104.00 104.00 104.00 104.00 104.00 104.00 L-Valine 94.00 94.00 94.00 94.00 94.00 94.00 CaCl2 (Anhydrous) 200.00 200.00 200.00 200.00 200.00 200.00 Fe(NO 3 ) 9H 2 0 0.10 0.10 0.10 0.10 0.10 0.10 KCl 400.00 400.00 400.00 400.00 400.00 400.00 MgSO 4 7H 2 O 199.99 199.99 199.99 199.99 199.99 199.99 NaHCO 3 3700.00 3700.00 3700.00 3700.00 3700.00 3700.00 NaCl 6400.00 6400.00 6400.00 6400.00 6400.00 6400.00 NaH 2 PO 4 125.00 125.00 125.00 125.00 125.00 125.00 Choline Chloride 4.00 4.00 4.00 4.00 4.00 4.00 D-Calcium Pantothenate 4.00 4.00 4.00 4.00 4.00 4.00 Folic Acid 4.00 4.00 4.00 4.00 4.00 4.00 i-inositol 7.20 7.20 7.20 7.20 7.20 7.20 Niacinamide 4.00 4.00 4.00 4.00 4.00 4.00 Pyridoxine HCl 4.00 4.00 4.00 4.00 4.00 4.00 Riboflavin 0.40 0.40 0.40 0.40 0.40 0.40 Thiamine HCl 4.00 4.00 4.00 4.00 4.00 4.00 D-Glucose (Dextrose) 4500.00 4500.00 4500.00 4500.00 1000.00 1000.00 Phenol Red 15.00 15.00 15.00 15.00 15.00 15.00 Sodium Pyruvate 110.00 110.00 0.00 0.00 110.00 110.00 DMEM, 4.5 g/l Glucose w/ Sodium Pyruvate and 500 VWRL0101-0500 Each w/ Sodium Pyruvate, w/out 500 VWRL0148-0500 Each w/out Sodium Pyruvate, w/ 500 VWRL0100-0500 Each w/out Sodium Pyruvate and 500 VWRL0102-0500 Each DMEM, 1.0 g/l Glucose w/ Sodium Pyruvate and 500 VWRL0111-0500 Each w/ Sodium Pyruvate, w/out 500 VWRL0149-0500 Each 2 VWR CELL CULTURE MEDIA AND REAGENTS PLACE YOUR ORDER TODAY AT VWR.COM, OR CALL 1.800.932.5000

RPMI-1640 RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth in many types of cultured cells. AMINO ACIDS INORGANIC SALTS VITAMINS OTHER Description RPMI 1640 w/ RPMI 1640 w/out Cat. No. VWRL0105-0500 VWRL0106-0500 Components mg/l mg/l Glycine 10.00 10.00 L-Arginine 200.00 200.00 L-Asparagine 50.00 50.00 L-Aspartic Acid 20.00 20.00 L-Cystine 2HCl 65.00 65.00 L-Glutamic Acid 20.00 20.00 300.00 0.00 L-Histidine 15.00 15.00 L-Hydroxyproline 20.00 20.00 L-Isoleucine 50.00 50.00 L-Leucine 50.00 50.00 L-Lysine HCl 40.00 40.00 L-Methionine 15.00 15.00 L-Phenylalanine 15.00 15.00 L-Proline 20.00 20.00 L-Serine 30.00 30.00 L-Threonine 20.00 20.00 L-Tryptophan 5.00 5.00 L-Tyrosine 2Na 2H 2 O 29.00 29.00 L-Valine 20.00 20.00 Ca(NO 3 ) 2 4H 2 O 100.00 100.00 MgSO 4 7H 2 O 100.00 100.00 KCl 400.00 400.00 NaHCO 3 2000.00 2000.00 NaCl 6000.00 6000.00 Na 2 HPO 4 7H 2 O 1512.00 1512.00 Biotin 0.20 0.20 Choline Chloride 3.00 3.00 D-Calcium Pantothenate 0.25 0.25 Folic Acid 1.00 1.00 i-inositol 35.00 35.00 Niacinamide 1.00 1.00 Para-Aminobenzoic Acid 1.00 1.00 Pyridoxine HCl 1.00 1.00 Riboflavin 0.20 0.20 Thiamine HCl 1.00 1.00 Vitamin B12 0.005 0.005 D-Glucose (Dextrose) 2000.00 2000.00 Glutathione (Reduced) 1.00 1.00 Phenol Red 5.00 5.00 RPMI 1640 w/ 500 VWRL0105-0500 Each RPMI 1640 w/out 500 VWRL0106-0500 Each PLACE YOUR ORDER TODAY AT VWR.COM, OR CALL 1.800.932.5000 VWR CELL CULTURE MEDIA AND REAGENTS 3

CELL CULTURE MEDIA AND REAGENTS BUFFERED SALT SOLUTIONS DULBECCO S PHOSPHATE BUFFERED SALINE (DPBS) Dulbecco s Phosphate Buffered Saline (DPBS) is a balanced salt solution used for the handling and culturing of mammalian cells. Phosphate buffering maintains the ph in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. INORGANIC SALTS Description DPBS (1X) w/ Ca and Mg DPBS (1X) w/out Ca and Mg Cat. No. VWRL0118-0500, VWRL0118-1000 Components g/l g/l CaCl 2 (Anhdyrous) 0.1 0 MgCl 2 6H 2 O 0.1 0 NaCl 8 8 KCl 0.2 0.2 Na 2 HPO 4 7H 2 O 2.16 2.16 KH 2 PO 4 0.2 0.2 VWRL0119-0500, VWRL0119-1000 DPBS (1X) w/ Ca and Mg 500 VWRL0118-0500 Each DPBS (1X) w/ Ca and Mg 1000 VWRL0118-1000 Each DPBS (1X) w/out Ca and Mg 500 VWRL0119-0500 Each DPBS (1X) w/out Ca and Mg 1000 VWRL0119-1000 Each HANK S BALANCED SALT SOLUTION (HBSS) Hank s Balanced Salt Solution (HBSS) is a buffer used to maintain a physiological ph for cells maintained in non-co 2 atmospheric conditions. HBSS can be used for washing cells or as a solution for transporting cells and tissue culture. INORGANIC SALTS HBSS (1X) w/out Ca, Mg, and Phenol Red VWRL0121-0500 Components g/l % of Solution NaCl 8 0.8% KCl 0.4 0.04% Na 2 HPO 4 (Anhydrous) 0.048 0.005% KH 2 PO 4 (Monobasic) 0.06 0.01% NaHCO 3 0.35 0.04% OTHER D-Glucose (Dextrose) 1 0.1% HBSS (1X) w/out Ca, Mg, and Phenol Red 500 VWRL0121-0500 Each 4 VWR CELL CULTURE MEDIA AND REAGENTS PLACE YOUR ORDER TODAY AT VWR.COM, OR CALL 1.800.932.5000

WATER, STERILE, LOW ENDOTOXIN, TISSUE CULTURE GRADE Water, sterile, low endotoxin, cell culture grade is ideal for use in the preparation of cell culture media and reagents. Test ph Sterility Total Organic Carbon (Before Packaging) Conductivity (Before Packaging) Endotoxin Check and Record Negative NMT 500 ppb NMT 1.3 µs/cm NMT 0.25 EU/mL Other Storage Conditions 15 C to 30 C For Further Manufacturing or Research Use. Not for Therapeutic Applications. Size Cat. No. Unit 500 ml VWRL0200-0500 Each 1 L VWRL0200-1000 Each AMINO ACIDS Amino acids supply nutrients to cells in culture supporting growth. Charged Amino Acids L-Arginine 100 g 97062-466 Each L-Arginine 500 g 97062-468 Each L-Aspartic Acid 500 g 97061-188 Each L-Aspartic Acid 1 kg 97061-186 Each L-Glutamic Acid 1 kg 97061-634 Each L-Glutamic Acid 2.5 kg 97061-636 Each L-Histidine 25 g 97063-020 Each L-Histidine 100 g 97063-018 Each L-Histidine 100 g 97062-598 Each L-Histidine 500 g 97062-602 Each L-Histidine 500 g 97064-022 Each L-Histidine 1 kg 97062-600 Each L-Lysine 100 g 97061-666 Each L-Lysine 500 g 97061-668 Each Hydrophobic Amino Acids L-Alanine 100 g 97061-024 Each L-Alanine 250 g 97061-026 Each L-Alanine 500 g 97061-028 Each L-Glycine, Biotechnology Grade 1 kg 97061-128 Each L-Glycine, Proteomics Grade 1 kg 97063-736 Each L-Glycine, Biotechnology Grade 5 kg 97061-132 Each L-Glycine, Proteomics Grade 5 kg 97063-738 Each L-Glycine 10 kg 97061-124 Each Hydrophobic Amino Acids L-Glycine 12 kg 97061-126 Each L-Glycine 50 kg 97061-130 Each L-Isoleucine 25 g 97064-016 Each L-Isoleucine 100 g 97064-014 Each L-Leucine 25 g 97064-034 Each L-Leucine 100 g 97064-030 Each L-Leucine 250 g 97063-032 Each L-Methionine 25 g 97064-010 Each L-Methionine 100 g 97063-008 Each L-Methionine 250 g 97063-012 Each L-Phenyalanine 25 g 97062-558 Each L-Phenyalanine 100 g 97062-556 Each L-Phenyalanine 500 g 97062-560 Each L-Proline 25 g 97064-038 Each L-Proline 100 g 97064-036 Each L-Proline 500 g 97064-040 Each L-Typtophan 25 g 97064-004 Each L-Typtophan 100 g 97064-002 Each L-Typtophan 500 g 97064-006 Each L-Valine 25 g 97062-564 Each L-Valine 100 g 97062-562 Each L-Valine 500 g 97062-566 Each PLACE YOUR ORDER TODAY AT VWR.COM, OR CALL 1.800.932.5000 VWR CELL CULTURE MEDIA AND REAGENTS 5

CELL CULTURE MEDIA AND REAGENTS REAGENTS AND SUPPLEMENTS ANTIBIOTICS Antibiotics are used to prevent contamination in cell culture. The use of antibiotics in cell culture is dependent on the needs of the researcher. Ampicillin, Gamma Irradiated, Tissue Culture 20 ml 97062-796 Each Grade Ampicillin, Sodium Salt, USP Grade 20 g 97061-442 Each Ampicillin, Sodium Salt, USP Grade 100 g 97061-440 Each Ampicillin, Trihydrate, USP Grade 50 g 97062-300 Each Ampicillin, Trihydrate, USP Grade 100 g 97062-296 Each Ampicillin, Trihydrate, USP Grade 500 g 97062-298 Each Carbenicillin, Disodium Salt, Ultra Pure Grade 250 mg 97063-146 Each Carbenicillin, Disodium Salt, Ultra Pure Grade 1 g 97063-144 Each Penicillin G, Sodium Salt, USP Grade 100 MU 97061-280 Each Penicillin G, Sodium Salt, USP Grade 1 BU 97061-282 Each Penicillin G, Sodium Salt, Irradiated, Tissue 2 ml 97062-798 Each Culture Grade Penicillin Streptomycin, Irradiated, Tissue Culture Grade 20 ml 97062-806 Each CITRIC ACID, TRISODIUM SALT, DIHYDRATE A biological buffer used for cell culture, in vitro, enzyme assays and some electrophoretic applications at physiological ph. Size Cat. No. Unit 500 g 97061-008 Each 1 kg 97061-004 Each 2.5 kg 97061-006 Each 12 kg 97061-002 Each 50 kg 97061-010 Each DIMETHYL SULFOXIDE, ULTRAPURE (DMSO) Used in cell culture applications >99.9% pure Sterile tested Dimethyl Sulfoxide, Ultrapure 5 x 10 ml 97063-136 Each L-GLUTAMINE L-glutamine is an essential amino acid. It is used as an energy source for cells in cell culture. L-glutamine is stable as a dry powder and as a frozen solution but degrades rapidly in liquid media or stock solutions. Components, 200mM (100X) VWRL0131-0100 g/l 29.2 NaCl 8.5, 200mM (100X) 100 VWRL0131-0100 Each GLYCEROL, STERILE, BIOTECHNOLOGY GRADE Cryoprotectant used in cell freezing Glycerol, Sterile, Biotechnology Grade 100 ml 97062-832 Each SERAFREE CRYOPRESERVATION MEDIA Sterile and endotoxin tested Reduces potential for transmission of infectious agents Eliminates regulatory compliance requirements associated with use of animal products Cost effective SeraFree Cryopreservation Media are ready-to-use freezing media for cryopreservation of adherent or suspension cultured cells. The animal-free RPMI- or DMEMbased media composition eliminates batch-to-batch variability and is optimized for cell viability and cell growth after thawing. Serafree Cryopreservation Media for DMEM 50 ml 97063-278 Each Serafree Cryopreservation Media for RPMI 6 x 5 ml 97064-278 Each Serafree Cryopreservation Media for RPMI 50 ml 97064-276 Each SODIUM CHLORIDE Sodium chloride is a common source of salts for media propagation of many organisms. Size Cat. No. Unit 500 g 97061-274 Each 1 kg 97061-270 Each 2.5 kg 97061-272 Each 5 kg 97061-278 Each 10 kg 97061-266 Each 12 kg 97061-268 Each 50 kg 97061-276 Each 100 kg 97061-264 Each 6 VWR CELL CULTURE MEDIA AND REAGENTS PLACE YOUR ORDER TODAY AT VWR.COM, OR CALL 1.800.932.5000

SUGARS High purity sugars are used for media supplements and are a primary source of energy for cells in culture. D-Fructose 1 kg 97061-236 Each D-Fructose 2.5 kg 97061-238 Each D-Fructose 5 kg 97061-242 Each D-Fructose 12 kg 97061-234 Each D-(+)-Galactose 100 g 97062-076 Each D-(+)-Galactose 250 g 97062-078 Each D-(+)-Galactose 500 g 97062-080 Each D- Anhydrous 500 g 97061-168 Each D- Anhydrous 1 kg 97061-164 Each D- Anhydrous 2.5 kg 97061-166 Each D- Anhydrous 5 kg 97061-172 Each D- Anhydrous 12 kg 97061-162 Each D- Anhydrous 50 kg 97061-170 Each 20% Solution, Sterile 100 ml 97062-880 Each Maltose Monohydrate 100 g 97062-604 Each Maltose Monohydrate 500 g 97062-608 Each Maltose Monohydrate 1 kg 97062-606 Each TRYPSIN (0.25%) EDTA Trypsin (0.25%) EDTA (1X) is used to release adherent cells from tissue culture plasticware for harvesting and passaging. Contains 0.001% phenol red, 0.04% Trypsin, 0.25% EDTA. INORGANIC SALTS OTHER Trypsin (0.25%) EDTA (1X) VWRL0154-0100 Components g/l % of Solution NaCl 8 0.8% KCl 0.4 0.04% Na 2 HPO 4 7H 2 O 0.091 0.009% KH 2 PO 4 (Monobasic) 0.06 0.01% NaHCO 3 0.35 0.04% Phenol Red 0.01 0.0010% EDTA 4Na 2H 2 O 0.38 0.04% Trypsin (1:250) 2.5 0.25% D-Glucose (Dextrose) 1 0.1% Trypsin (0.25%) EDTA (1X) 100 VWRL0154-0100 Each ALL YOU NEED Solutions for Science At VWR it is our mission to enable the advancement of science throughout the world. We do this by developing deep connections with our customers. We have a commitment to provide the best-in-class product portfolio, services, and operational efficiency to ensure that our customers can focus on their research. All You Need Solutions are a testament to this mission. We now offer a comprehensive range of products and services suited to a specific industry or application. Whether you are working in the Life Science, Analytical, or Production environments we have programs designed for you. Find out how VWR is helping the scientific community with All You Need Solutions! vwr.com/aynsolutions PLACE YOUR ORDER TODAY AT VWR.COM, OR CALL 1.800.932.5000 VWR CELL CULTURE MEDIA AND REAGENTS 7

TISSUE CULTURE PLASTICWARE VWR Collection Cell Culture Volume and Area Guide Description VWR Standard Line Multiwell Cell Culture Plates Size Working Volume, ml Cell Growth Area, cm 2 Max Cell Yield** Cat. No. 6 Well 17.0 ml 1.90-2.90 9.6 9.6 x 10 5 10062-892 12 Well 6.80 ml 0.76-1.14 3.85 3.85 x 10 5 10062-894 24 Well 3.50 ml 0.38-0.57 1.93 1.93 x 10 5 10062-896 48 Well 1.55 ml 0.19-0.29 0.84 8.4 x 10 4 10062-898 96 Well, Flat Bottom 96 Well, U Bottom 0.39 ml 0.075-0.20 0.33 3.3 x 10 4 10062-900 0.32 ml 0.075-0.20 0.32 3.2 x 10 4 10062-902 Crystal clear virgin PS - sterile and certified non-pyrogenic Plasma treated for consistent cell attachment and growth Well volume uniformity ensures uniform growth surface area exposure Single position lid reduces misplacement and the risk of contamination VWR Standard Line Cell Culture Dishes VWR Standard Line Cell Culture Dishes, Gripping Ring for Improved Handling VWR Standard Line Cell Culture T-Flasks, Non-vented Caps 35 Dia. x 12 H, mm 1.7-4.25 8.5 8.5 x 10 5 10062-888 Crystal clear virgin PS - sterile and 90 Dia. x 17 H, mm 11-27.5 55 5.5 x 10 6 10062-878 certified non-pyrogenic Plasma treated for consistent cell 150 Dia. x 22 H, mm 60 Dia. x 18 H, mm 70 Dia. x 15 H, mm 100 Dia. x 22 H, mm 28.6-71.5 4.24-10.6 7.26-18.15 12.16-30.4 143 21.2 36.3 60.8 1.43 x 10 7 2.12 x 10 6 3.63 x 10 6 6.08 x 10 6 10062-882 10062-890 10062-876 10062-880 attachment and growth Flat bottom and uniform wall thickness ensure dishes are distortion-free Optically clear, making them suitable for microscopy 12.5 cm 2 (25 ml) 2.5-6.25 12.5 1.25 x 10 6 10062-870 Angled neck design offers good pipette 25 cm 2 (50 ml) 5-12.5 25 2.5 x 10 6 10062-874 and cell scraper access 75 cm 2 (250 ml) 15-37.5 75 7.5 x 10 6 10062-862 Plasma treated for consistent cell attachment and growth 182.5 cm 2 (600 ml) 36.5-91.25 182.5 1.83 x 10 7 10062-866 Crystal clear virgin PS - sterile and 300 cm 2 (850 ml) 60-150 300 3 x 10 7 10062-886 certified non-pyrogenic Also available with vented caps VWR Roller Bottles, Surface Treated 2/Bag, 40/Case 1,000 ml 100-150 490 4.9 x 10 7 10769-794 2/Bag, 20/Case 2,000 ml 175-250 850 8.5 x 10 7 10769-796 5/Bag, 40/Case 2,000 ml 175-250 850 8.5 x 10 7 10769-798 2/Bag, 40/Case 2,000 ml 175-250 850 8.5 x 10 7 10769-800 5/Bag, 40/Case 2,000 ml 380-570 (Ribbed Design) 1900 1.9 x 10 8 10769-802 Sterilized, non-pyrogenic Bottle surface is smooth and free from striation to maximize usable growth area Two different cap styles can be used in both open and closed systems Cat. No. 10769-802 features a rib design VWR Erlenmeyer Flasks, Flat Base, Non-vented Caps VWR Erlenmeyer Flasks, Baffled Base*, Non-vented Caps * Baffled base flasks may allow higher working volumes or low shaking speeds ** Based on cell diameter 10µm 125 ml 21.3-50 89095-260 250 ml 62.5-100 89095-268 500 ml 125-200 89095-276 1 L 250-400 89095-284 2 L 500-800 10126-438 3 L 750-1200 10126-410 125 ml 21.3-50 89095-264 250 ml 62.5-100 89095-272 500 ml 125-200 89095-280 1 L 250-400 89095-288 2 L 500-800 10126-408 3 L 750-1200 10126-412 Virgin, medical-grade resin retains its clarity after being exposed to gamma radiation Non-pyrogenic, inert, and nonleaching Excellent for non-adhering/suspension cultures Also available with vented caps 2L and 3L flasks with convenient vented duo-cap makes switching caps easy; simply remove the dustguard cover to allow sterile exchange, then replace the cover 0616 5M Lit. No. 93612