Analytical Reference Materials European OPP Mix Catalog # 32418 Lot # A0104957 110 Benner Circle Bellefonte, PA 16823-8812 1-814-353-1300 1-800-356-1168 www.restek.com FOR LABORATORY USE ONLY. READ MSDS PRIOR TO USE. RAW MATERIAL TEST INFORMATION AVAILABLE UPON REQUEST MANUFACTURED UNDER RESTEK'S ISO 9001 REGISTERED QUALITY SYSTEM Page 1 of 7
CERTIFIED REFERENCE MATERIAL 110 Benner Circle Bellefonte, PA 16823-8812 Tel: (800)356-1688 Fax: (814)353-1309 Certificate of Analysis www.restek.com FOR LABORATORY USE ONLY-READ SDS PRIOR TO USE. This Reference Material is intended for Laboratory Use Only as a standard for the qualitative and/or quantitative determination of the analyte(s) listed. Catalog No. : 32418 Lot No.: A0104957 Description : European OPP Mix Container Size : Organophosphorous Pesticide Mix European Formulation 100-1000µg/mL, Acetone, 1mL/ampul 2 ml > 1 ml Pkg Amt: Expiration Date : July 31, 2015 Storage: 10 C or colder Handling: This product is photosensitive. C E R T I F I E D V A L U E S Elution Order Compound Grav. Conc. (weight/volume) 1 Methamidophos 503.0 µg/ml CAS # 10265-92-6 (Lot 10711) 2 Dichlorvos (DDVP) 502.7 µg/ml CAS # 62-73-7 (Lot 2144700) Purity 98% 3 Acephate 201.0 µg/ml CAS # 30560-19-1 (Lot 2442600) 4 Omethoate 1,001.3 µg/ml CAS # 1113-02-6 (Lot 2729200) Purity 96% 5 Demeton-s-methyl 201.4 µg/ml CAS # 919-86-8 (Lot 1958600) Purity 95% 6 Dimethoate 201.0 µg/ml CAS # 60-51-5 (Lot 2349900) 7 Tolclofos-methyl 100.0 µg/ml CAS # 57018-04-9 (Lot 306500) 8 Pirimiphos methyl 100.0 µg/ml CAS # 29232-93-7 (Lot 2565100) Expanded Uncertainty (95% C.L.; K=2) +/- 2.9877 µg/ml +/- 18.1214 µg/ml +/- 18.1220 µg/ml +/- 2.9861 µg/ml +/- 18.1120 µg/ml +/- 18.1126 µg/ml +/- 1.4253 µg/ml +/- 7.2831 µg/ml +/- 7.2833 µg/ml +/- 5.9473 µg/ml +/- 36.0727 µg/ml +/- 36.0739 µg/ml +/- 1.4281 µg/ml +/- 7.2976 µg/ml +/- 7.2978 µg/ml +/- 1.4253 µg/ml +/- 7.2831 µg/ml +/- 7.2833 µg/ml +/- 0.7091 µg/ml +/- 3.6234 µg/ml +/- 3.6235 µg/ml +/- 0.7091 µg/ml +/- 3.6234 µg/ml +/- 3.6235 µg/ml Page 2 of 7
9 Malathion 202.0 µg/ml CAS # 121-75-5 (Lot 2131600) 10 Chlorpyrifos 101.0 µg/ml CAS # 2921-88-2 (Lot 1583700) 11 Methidathion 199.8 µg/ml CAS # 950-37-8 (Lot 2334000) Purity 97% 12 Prothiofos 200.8 µg/ml CAS # 34643-46-4 (Lot 2552100) Purity 97% 13 Profenofos 200.6 µg/ml CAS # 41198-08-7 (Lot 2593700) Purity 96% 14 Ethion 200.0 µg/ml CAS # 563-12-2 (Lot 2682000) 15 Azinphos methyl 400.0 µg/ml CAS # 86-50-0 (Lot 30415) Purity 95% 16 Pyrazophos 504.0 µg/ml CAS # 13457-18-6 (Lot 2612100) +/- 1.4323 µg/ml +/- 7.3193 µg/ml +/- 7.3195 µg/ml +/- 0.7162 µg/ml +/- 3.6596 µg/ml +/- 3.6598 µg/ml +/- 1.4169 µg/ml +/- 7.2403 µg/ml +/- 7.2406 µg/ml +/- 1.4238 µg/ml +/- 7.2755 µg/ml +/- 7.2757 µg/ml +/- 1.4227 µg/ml +/- 7.2700 µg/ml +/- 7.2703 µg/ml +/- 1.4182 µg/ml +/- 7.2468 µg/ml +/- 7.2471 µg/ml +/- 2.8360 µg/ml +/- 14.4918 µg/ml +/- 14.4923 µg/ml +/- 2.9936 µg/ml +/- 18.1574 µg/ml +/- 18.1580 µg/ml Solvent: Acetone CAS # Purity 67-64-1 99% Page 3 of 7
Column: 30m x 0.25mm x 0.25µm Rtx-5 (cat.#10223) Carrier Gas: hydrogen-constant pressure 10 psi Temp. Program: 35 C (hold 3 min.) to 330 C @ 3 C/min. (hold 3 min.) Inj. Temp: 250 C Det. Temp: 300 C Det. Type: FID This chromatogram represents a general set of testing conditions chosen for product acceptance. For optimal results in your lab, conditions should be adjusted for your specific instrument, method, and application. Date Mixed: 29-Jul-2014 Balance: 1128342313 Date Passed: 04-Aug-2014 Manufactured under Restek's ISO 9001:2008 Registered Quality System Certificate #FM 80397 Page 4 of 7
General Certified Reference Material Notes Expiration Notes: Expiration date valid for unopened ampul stored in compliance with the recommended conditions. Uncertainty, concentration, and expiration of the CRM are based on the unopened product being stored according to the recommended condition found in the storage field. Purity Notes: Purity and/or chemical identity are determined by one or more of the following techniques: GC/FID, HPLC, GC/µECD, GC/MS, LC/MS, RI, and/or melting point. Compounds with a listed purity of less than 99% have been weight corrected to compensate for impurities and/or salts. A correction factor is used to calculate the amount of compound necessary to achieve the desired concentration of the parent compound in solution. Purity of isomeric compounds is reported as the sum of the isomers. Purity values are rounded to the nearest whole number. Certified Uncertainty Value Notes: The uncertainties are determined in accordance with ISO Guides 34 and 35. The certified combined stressed uncertainty value ( includes gravimetric uncertainty, homogeneity between-ampul uncertainty, storage stability uncertainty and shipping stability uncertainty and were combined using the following formula: k is a coverage factor of 2, which gives a level of confidence of approximately 95%. It is important to note that the shipping stability uncertainty was obtained under temperature extremes for specific time intervals; therefore, the certified combined stressed uncertainty value should only be applied to the product if it was stored at non-standard temperature conditions up to and including 7 days. Contact Restek Technical Service at www.restek.com/contact-us for use recommendations if your shipment was in-transit for more than 7 days at nonstandard temperature conditions. Apply the certified combined unstressed uncertainty value if the product was received under standard shipping conditions. Apply the certified combined stressed uncertainty value if the product was received under non-standard conditions as specified below. Label Conditions 25 C Nominal (Room Temperature) 10 C or colder (Refrigerate) 0 C or colder (Freezer) Standard Conditions < 60 C < 40 C < 25 C Non-Standard Conditions 60 C up to 7 days 40 C up to 7 days 25 C up to 7 days Separate (not combined) uncertainty values for gravimetric uncertainty are also displayed on the certificate, if needed, separate homogeneity between-ampul uncertainty, storage stability uncertainty and shipping stability uncertainty values are available by contacting Restek Technical Service at www.restek.com/contact-us. The packaged amount is the minimum sample size for which uncertainty is valid. The ampules are over-filled to ensure that the minimum packaged amount can be sufficiently transferred. Manufacturing Notes: Concentration is based upon gravimetric preparation using either a balance whose calibration has been verified daily using NIST traceable weights, and/or dilutions with Class A glassware. Handling Notes: Samples should be transferred into deactivated vials for handling and storage. Restek supplies deactivated vials along with most standards packed in 2 ml ampules. Due to space constraints, Restek does not supply vials for larger volume ampules. Restek sells DMDCS for the purpose of glassware deactivation as catalog number 31840, which includes complete instructions. Restek will also deactivate larger volume vials from our inventory as a custom ordered item. Contact your Restek sales or customer service representative for details. If any undissolved material is visible inside the ampul, sonicate the unopened ampul until the material is completely dissolved. Page 5 of 7
Catalog #: 32418 Target: 100-1000 ug/ml Description: European OPP Mix Solvent: Acetone Solvent Lot: 0000071000 Chemical Standard Batch Sheet Lot #:A0104957 Final Volume: 100 ml Made by: Cheryl Graham Date: 7/29/2014 7:54:17PM Tested by: Diane Shaffer Pass Date: 7/30/2014 11:21:47AM By: Diane Shaffer Date: 8/4/2014 11:32:56AM Packaged by: Lane Kibe / Lane Kibe Date: 7/29/2014 10:09:21PM No. Units: 66 Pkg Size: 1 ml Balance Used: BEDEARMBALPC1 XP205 Serial #: 1128342313 Compound CAS Pyrazophos 13457-18-6 Chlorpyrifos 2921-88-2 Malathion 121-75-5 Dimethoate 60-51-5 Azinphos methyl 86-50-0 Dichlorvos (DDVP) 62-73-7 Pirimiphos methyl 29232-93-7 Ethion 563-12-2 Prothiofos 34643-46-4 Demeton-s-methyl 919-86-8 Omethoate 1113-02-6 Methidathion 950-37-8 Methamidophos 10265-92-6 Acephate 30560-19-1 Profenofos 41198-08-7 Tolclofos-methyl 57018-04-9 Storage Target Location Lot # Purity Conc(ug/mL) Target Actual Calc Conc(ug/mL) R0450 2612100 0.99 500.00 50.00 mg 50.40 mg 504.0 R0557 1583700 0.99 100.00 10.00 mg 10.10 mg 101.0 R0568 2131600 0.99 200.00 20.00 mg 20.20 mg 202.0 R0586 2349900 0.99 200.00 20.00 mg 20.10 mg 201.0 R0592 30415 0.95 400.00 42.11 mg 42.10 mg 400.0 R0593 2144700 0.98 500.00 51.02 mg 51.30 mg 502.7 R0600 2565100 0.99 100.00 10.00 mg 10.00 mg 100.0 R0603 2682000 0.99 200.00 20.00 mg 20.00 mg 200.0 R0741 2552100 0.97 200.00 20.62 mg 20.70 mg 200.8 R0762 1958600 0.95 200.00 21.05 mg 21.20 mg 201.4 R0793 2729200 0.96 1,000.00 104.17 mg 104.30 mg 1,001.3 R0794 2334000 0.97 200.00 20.62 mg 20.60 mg 199.8 R0798 10711 0.99 500.00 50.00 mg 50.30 mg 503.0 R0807 2442600 0.99 200.00 20.00 mg 20.10 mg 201.0 R0819 2593700 0.96 200.00 20.83 mg 20.90 mg 200.6 R0988 306500 0.99 100.00 10.00 mg 10.00 mg 100.0 Page 6 of 7
QA Report: European OPP Mix (Cat.#32418) Runs of Lot # A0104957 Runs of Lot # A099346 COMPONENT Run #1 Run #2 Run #3 AVG STD DEV % RSD Run #1 Run #2 Run #3 AVG STD DEV % RSD %D MEAN P/F Methamidophos 41163 40980 40431 40858 381 0.93 40849 40494 40169 40504 340 0.84 0.87 PASS Dichlorvos 50747 50136 49364 50082 693 1.38 50526 50046 49148 49907 699 1.40 0.35 PASS Acephate 20214 20036 19821 20024 197 0.98 21092 21081 20870 21014 125 0.60-4.95 PASS Omethoate 100277 100006 98269 99517 1090 1.09 99254 98114 97268 98212 997 1.01 1.31 PASS Demeton-s-methyl 30738 30538 30010 30429 376 1.24 30804 30514 30085 30468 362 1.19-0.13 PASS Dimethoate 19866 19968 19488 19774 253 1.28 19975 19763 19813 19850 111 0.56-0.39 PASS Tolclofos-methyl 18708 18402 18355 18488 192 1.04 18423 18350 18284 18352 70 0.38 0.74 PASS Pirimiphos-methyl 21392 21099 20857 21116 268 1.27 21050 21012 20650 20904 221 1.06 1.00 PASS Malathion 27707 27480 26822 27336 460 1.68 26653 26318 26106 26359 276 1.05 3.58 PASS Chlorpyrifos 16480 16408 16075 16321 216 1.32 16225 16050 15916 16064 155 0.96 1.58 PASS Methidathion 17061 17111 17027 17066 42 0.25 16401 16163 16119 16228 152 0.93 4.91 PASS Prothiofos 37614 37358 36820 37264 405 1.09 37256 36924 36537 36906 360 0.98 0.96 PASS Profenofos 35923 35698 35187 35603 377 1.06 33671 33171 32859 33234 410 1.23 6.65 PASS Ethion 26214 26283 25927 26141 189 0.72 26578 26141 26211 26310 235 0.89-0.65 PASS Azinphos methyl 64493 64797 64384 64558 214 0.33 64155 63707 63965 63942 225 0.35 0.95 PASS Pyrazophos 86549 86894 86101 86515 398 0.46 90090 88885 89884 89620 645 0.72-3.59 PASS Page 7 of 7 8/4/2014 11:30:44 AM 1/1-1