2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Department of Cellular Pathology Contact: Karen Wignall Royal Preston Hospital Tel: +44 (0) 1772 523108 Sharoe Green Lane E-Mail: karen.wignall@lthtr.nhs.uk Fulwood Website: http://www.lancsteachinghospitals.nhs.uk Preston PR2 9HT Testing performed at the above address only Laboratory locations: Location details Activity Address Department of Cellular Pathology Royal Preston Hospital Sharoe Green Lane Fulwood Preston PR2 9HT United Kingdom Local contact Karen Wignall Histology Electron Microscopy Neuropathology Immunocytochemistry Non-Gynae Cytology Mortuary services Site activities performed away from the locations listed above: Location details Activity Mortuary Department Chorley & South Ribble Hospital Preston Road Chorley PR7 1PP United Kingdom Mortuary services Assessment Manager: GM3 Page 1 of 16
DETAIL OF ACCREDITATION Fixed, fresh and frozen tissue including excisional and incisional biopsies and surgical resection specimens Fixed, fresh and frozen tissue including excisional and incisional biopsies and surgical resection specimens Formalin- fixed and processed tissue (to paraffin wax) including excisional and incisional biopsies and surgical resection specimens tissue blocks For demonstration of general tissue architecture including basophilic and eosinophilic structures Acid Mucopolysaccharides Specimen Dissection Manual Methods SP 440 Tissue Processing Peloris II Tissue Processor SP331 Tissue Embedding 1. Surgipath Premier Series 2. Shandon Histocentre 2 3. Leica EG1150 H SP309 Tissue Sectioning General Laboratory: 1. Leica RM2235 2. Leica RM2135 3. Leica RM2135 4. Leica RM2135 5. Leica RM2235 6. Leica RM2235 Neuropathology: 1. Leica RM2235 2. Leica RM2235 SP296 Haematoxylin and Eosin Staining Manual Technique SP10 Automated Technique using Leica CV5030 Autostainer SP376 Special Stains Manual Techniques TP48, TP49 +/- Automated Technique using Ventana Benchmark and the following SOPs: SP2 (H/A) Alcian Blue Assessment Manager: GM3 Page 2 of 16
Esterase activity Nuclei Mast cells and neutrophils Myeloid cells Amyloid Elastic tissue fibres Mast Cells Helicobacter pylori Gram positive and Gram negative organisms Argyrophil cells Fungi General tissue architecture including basophilic and eosinophilic structures Elastic fibres and connective tissue Removal of tinctorial stains/pigments Melanin pigment Connective tissue Fibrin Special Stains Manual Techniques TP48, TP49 +/- Automated Technique using Ventana Benchmark and the following SOPs: SP4 (H) Chloroacetate Esterase SP4 (H/A) Congo Red Improved Method SP6 (H) Miller s Elastic Van Gieson (EVG) SP44 (H/A) Rapid Giemsa SP48 (H/A) Gram-Twort SP49 (H) Grimelius Silver Method SP9 (H/A) Grocott- Gomori Methenamine Silver SP11 (H) Rapid H&E SP13 (H) Haematoxylin Van Gieson SP53 (H) Mallory Bleach SP16 (H/A) Masson Fontana SP54 (H/A) Masson Trichrome SP18 (H/A) Martius Scarlet Blue (MSB) Assessment Manager: GM3 Page 3 of 16
Simple lipids Hepatitis B surface antigen PAS positive structures e.g. glycogen, cells of pituitary Haemosiderin and ferric iron Removal of formalin pigment Reticulin fibres Metachromatic substances Calcium Campylobacter sp. Tubercle bacilli (TB) Epithelial cells Hepatocytes and germ cell tumour Lymphoma Deficiency of alpha-1-antitrypsin Secondary amyloid Special Stains Manual Techniques TP48, TP49 +/- Automated Technique using Ventana Benchmark and the following SOPs: SP20 (H) Oil Red O SP67 (H/A) Orcein SP21 (H/A)Periodic Acid Schiff (PAS) +/- Diastase SP23 (H/A) Perl s Prussian Blue SP23 (H) Removal of formalin pigment SP26 (H/A)Gordon & Sweet s Reticulin Method SP88 (H) Toluidine blue SP89 (H/A) Von Kossa SP91 (H/A) Warthin Faulkner SP28 (H/A)Ziehl- Neelsen (ZN) AE1/AE3 Alphafetoprotein ALK1 Alpha-1-antitrypsin Amyloid A Assessment Manager: GM3 Page 4 of 16
Prognosis in malignant glioma Prostate Scattered T cells in germinal centres and mantle zone B cellsfollicular lymphoma Lymphoma Renal complement Clear cell v renal cell carcinoma GI tumours/ Pancreas Ovarian tumours Thyroid C cells and carcinoma Smooth muscle and sarcoma Mesothelial and lung tumours ATRX 34 BE12 BCL2 BCL6 C3c CAIX Ca19-9 CA125 Calcitonin CALDESMON CALRETININ Cytokeratin low MWT CAM 5.2 T Cells in mantle zone T Cells T and B cells- leukaemia T Cells T Cell malignancy Childhood ALL, follicular lymphoma CD3 CD4 CD5 CD8 CD7 CD10 Assessment Manager: GM3 Page 5 of 16
Reed Sternberg cells, Hodgkin s and meso panel B Cells - lymphoma B Cells- dendritic cells Lymphocytes in germinal centres T Cells- inflammatory and malignancy Reed Sternberg cells, Hodgkin s Endothelial cells- malignancy Vascular epithelium T Cell lymphoma Breast and lung carcinoma Neuroectodermal cells, neurones, astrocytes, Neuroectodermal cells and tumours Megakaryocytes in trephines Macrophages B Cells Ewing s sarcoma Endothelial cells GIST tumours CD15 Leu M1 CD20 CD21 CD23 CD25 (Interleukin-2) CD30 (Ki) CD31 CD34 (Q bend/10) CD43 CD44 CD56 CD57 (NK-1) CD61- separate control CD68 CD79a CD99 (MIC2) CD105 CD117 (c-kit) Assessment Manager: GM3 Page 6 of 16
B Lymphocyte differentiation Differentiation of carcinoids and metastatic adenocarcinoma Epithelial malignancy Prostate Ovarian Stratified epithelium Ductal and glandular epithelium Ovarian Used in panels of several malignancies e.g. colon, breast ductal Mantle cell lymphoma Cytomegalovirus Hairy cell leukaemia Smooth and striated muscle GIST tumours Epstein- Barr virus Epithelial cell carcinoma Epithelial cells CD138 CDX-2 CEA CK5/6 CK7 CK14 CK19 CK20 COX-2 CYCLIN D1 CMV DBA44 DESMIN DOG-1 EBV E-CADHERIN EMA Assessment Manager: GM3 Page 7 of 16
Endothelial cells and megakaryocytes Breast Ca Breast Ca Mesothelial cells FACTOR 8 (Von Willebrand Factor) GATA3 GCDFP-15 Gross cystic disease fluid protein HBME-1 Hepatitis B HBS (Hep B) Germ cell tumours Hepatocytes Skeletal, cardiac and smooth muscle Herpes virus type 8 B Cells HCG HeppAR1 HHF 35 HHV8-LNA HLA (MHC) Melanocytes HMB 45 Human papilloma virus Herpes simplex virus Renal Renal Renal Tumour classification and identifying single invasive cells in gliomas HPV HSV IgA IgG IgM IDH1 Assessment Manager: GM3 Page 8 of 16
Sex cord stromal tumours Light chains Light chain Lymphoid Prostate epithelium Breast Ca Melanocytes B Cell lymphoma Skeletal muscle Skeletal and cardiac cells Neuronal Peripheral nerves Neuroendocrine Melanoma cells B Cell lymphoma Germ cell tumour Breast ca Cervical ca Various cancers INHIBIN KAPPA LAMBDA LCA (CD45 PD7-2B11) LP34 Mammaglobin MELAN A MUM-1 MYOGENIN MYOGLOBIN NEUROFILAMENT (Invitrogen) NEUROFILAMENT (Dako) NSE NK1/C3 OCT2 OCT3/4 OESTROGEN P16 P53 Assessment Manager: GM3 Page 9 of 16
Tumour suppressor Prostate adeno Thyroid Neurons Germ cell tumours and testicular ca Breast Prostate neoplasm Renal cell ca Melanoma, glial cells Smooth muscle Smooth muscle myosin Melanoma marker- especially spindle cell type T and B Cells in leukaemia Vascular tumours Thyroid Lung and thyroid Mesenchymal cells Renal tumours, mesothelioma P57 P5045 (α Methyl CoA) PAX-8 PGP9-5 PLACENTAL AP PROGESTERONE- AB Leica Progesterone PgR636- Dako PSA RCC S100 SMA SMM SOX-10 TDT THROMBOMODULIN (CD141) THYROGLOBULIN TTF-1 VIMENTIN VIMENTIN Assessment Manager: GM3 Page 10 of 16
Plaques and tangles Nerve axons Myelin Myelin and other CNS structures Neurones Acidophils and basophils in the pituitary Muscle striations and astrocytes in CNS tissue Neuropathology Manual Staining with reference to: SP3 Bielschowsky Stain- modified SP12 HRS- Modified Silver Stain for Nerve Axons SP14 Luxol Fast Blue SP15 Luxol fast Blue H&E SP19 Nissl Demonstration SP22 PAS-Orange G Method for Pituitary SP24 PTAH Frozen Sections Mitochondrial complexes SP42 Gomori Trichrome Stain Frozen Sections Lipids SP27 Sudan Black for Lipids Frozen Sections Neuropathology Enzyme Histochemistry using manual staining with reference to the following Lysosomes in muscle Mitochondrial complexes Mitochondrial complexes Fibre typing Fibre typing Fibre typing SP66 Acid Phosphatase SP38 Cytochrome Oxidase SP41 Cytochrome Oxidase and SDH SP46 Myoadenylate Deanimase SP57 Myosin ATPase SP56 NADH for Type 1 Fibres Assessment Manager: GM3 Page 11 of 16
Frozen Sections Neuropathology Enzyme Histochemistry using manual staining with reference to the following : Frozen Sections Phosphorylase activity Stain- glycogen Phosphofructokinase and aldolase activity SDH Activity Myelin Connective tissue Myelin For localisation of proteins in muscle SP59 Phosphorylase SP61 PAS for Muscle Biopsies SP64 Phosphofructokinase and Aldolase SP66 Succinic Dehydrogenase SP78 Kultschitsky SP79 Goldner s Trichrome SP80 Solochrome Cyanine Neuropathology using SP90 manual staining with reference to the following Adhalin B Dystroglycan - frozen Dysferlin Dystrophin 1 Dystrophin 2 Dystrophin 3 Emerin Merosin 300 Assessment Manager: GM3 Page 12 of 16
Frozen Sections For localisation of proteins in muscle Pituitary hormone Neuro protein Neuro protein Neuro protein Neuro protein Membrane attack complex Pituitary hormone Pituitary hormone Complement protein Complement protein Malignancy marker brain tumour Pituitary hormone Membrane attack complex Malignancy marker brain tumour Neuropathology using SP90 manual staining with reference to the following Merosin 80 Spectrin Neuropathology ACTH α- SYNUCLEIN Alpha beat crystalline ANTI-ALZ BETA AMYLOID C5b-9 FSH GH HLA Class I HLA Class II IDH LH MAC C5b-9- membrane attack complex- frozen MGMT Assessment Manager: GM3 Page 13 of 16
Surgical Biopsies Muscle fibre type Muscle fibre type Muscle fibre type Muscle fibre type Neuronal marker Neuronal marker Inclusion body myositis Pituitary marker Tumour marker Tangles in neurones Pituitary Fibre typing Neuronal marker Neuropathology Myosin heavy chain developmental Myosin heavy chain fast Myosin heavy chain neonatal Myosin heavy chain slow Nestin- paraffin NOS-1 (nitrous oxide) P63 PROLACTIN SYNAPTOPHYSIN TAU TSH TROPONIN T Utrophin- frozen Electron Microscopy Electron Microscopy (EM) Preparation with reference to the following: SP-451 Collecting Specimens for EM Assessment Manager: GM3 Page 14 of 16
Surgical Biopsies Surgical Biopsies Examination of tissues to identify or exclude ultrastructural morphological abnormalities for the purpose of Examination of tissues to identify or exclude ultrastructural morphological abnormalities for the purpose of Electron Microscopy Electron Microscopy (EM) Preparation with reference to the following: SP-473 Processing EM Tissues SP-471 Phosphate Buffer ph7 for EM SP-404 Embedding EM Specimens SP-406 Cutting of EM Specimens SP-482 Making of Toluidine Blue for Semi Thins SP-407 EM Toluidine Blue Labels SP-622 Training Log for ICC-EM SP-413 Filing of EM Grids, Blocks and Slides TP-17 Operation of Ultramicrotome TP-52 Embedding and Processing Electron Microscopy Electron Microscopy Viewing with reference to the following: SP-481 Operation of Transmission Electron Microscope (including reporting) SP-624 Electron Microscopy Checking TP-16 Operation of Transmission Electron Microscope Assessment Manager: GM3 Page 15 of 16
Diagnostic Cytology Samples including fluids, FNAs and Brushings Alcohol- Fixed Cytological Preparations on Microscope Slides Alcohol- Fixed Cytological Preparations on Microscope Slides Air- Dried Cytological Preparations on Microscope Slides Diagnostic Cytology Samples including fluids, FNAs and Brushings Stained Tissue Sections/ Stained Cytological Preparations Differentiation of cell types and identification of normal and abnormal cells Differentiation of cell types and identification of normal and abnormal cells Differentiation of cell types Microscopic identification of cellular structures and abnormality for the purpose of clinical. Diagnostic Cytology SP195 Preparation of Slides for Staining using Shandon Cytospin Diagnostic Cytology SP511 Staining of Cytological Preparations using Stainmate for: Haematoxylin and Eosin Stain Papanicolaou Stain Diagnostic Cytology SP607 Manual Stain using Rapid Giemsa Diagnostic Cytology SP347 Preparation of Cell Block for Histological Examinations Consultants and BMS Reporting Cellular Pathology Reporting SP 634 Reporting of Results Supplementary, Amended, Clinical Advice and Referred Work SP-439 BMS Reporting Non-Gynae Specimens SP-268 END Assessment Manager: GM3 Page 16 of 16