PAIN PANEL. Application Guide. Screen 41 compounds in under 4 minutes

Application Guide PAIN PANEL Screen 41 compounds in under 4 minutes Solutions for large compound screens and individual compound classes such as benzodiazepines, opiates, and more Achieve resolution of critical isobars Simplified, rapid cleanup procedures

Here to Support You with Your Clinical Research Personalized Support Your lab has its very own Technical Consultant that is available to assist with method development, optimization, troubleshooting, and product recommendations. Feel free to give them a call with any questions you may have! Get to Know Your Technical Consultant 31-212- Take Advantage of Our Lab Our customers asked us for more support, we responded with PhenoLogix sm, a full-service analytical support laboratory. Let our PhenoLogix clinical research gurus save your lab time and money. Method development and optimization in our PhenoLogix laboratory On-site method development On-site hands-on training Explore PhenoLogix www.phenomenex.com/phenologix 24 Hour Access to Information Technical Database Access our vast database of technical notes, applications, webinars, and more. Search by industry, document type, and separation mode to easily find what you need. www.phenomenex.com/mylinks Tools Utilize our web tools to find product recommendations, create customized methods, and more. www.phenomenex.com/tools For Research Use Only. Not for use in diagnostic procedures. 214 Phenomenex, Inc. All rights reserved.

TABLE OF CONTENTS Simple Sample Preparation Solid Phase Extraction (SPE)...4- Protein Precipitation (PPT)...4- Full Pain Panel Screen Choose from 3 different core-shell stationary phases to customize your work Biphenyl, Greatest Versatility and Performance...6-7 Phenyl-Hexyl, imethod Tested...8-9 C18, General Purpose... 1 Analyze Individual Classes of Drugs Benzodiazepines... 11 Opiates... 12 Drugs of Abuse... 13 Ordering Information...14-1 3

Guaranteed Solutions Effective and highly reproducible methodology helps you ensure results and reduce costs. With guaranteed high quality products from Phenomenex, you can easily push your research to the next level. We guarantee that if Phenomenex products in this guide do not provide at least an equivalent separation as compared to other products of the same phase and dimensions, send in your comparative data within 4 days and keep the Phenomenex product for FREE! Solid Phase Extraction (SPE) Strata -X-Drug B Protein Precipitation (PPT) Impact Designed and quality controlled for basic drugs of abuse Time and solvent saving polymeric strong cation-exchange SPE sorbent No equilibration or condition steps needed Complete in plate removal of proteins (b-glucuronidase) Increase throughput 3x Leak-free oleophobic membrane Easily automated Part no. CE-766 HPLC/UHPLC Analysis Kinetex Biphenyl Core-Shell Technology Rugged and reliable core-shell columns Incredible performance and efficiencies TMS TMS Biphenyl Enhanced polar and basic retention Quality controlled for basic drugs of abuse 1.7 µm 2.6 µm µm 4

Rapid, Simple Cleanup for Pain Management Drugs and Drugs of Abuse Skip the method development, significantly reduce your sample preparation time, and save solvent using Strata -X-Drug B SPE tubes and 96-well plates. One method is all you will need to extract over 41 pain management drugs. Or easily upgrade the throughput of your existing protein precipitation methods with the new Impact Protein Precipitation long drip plates. Sample Pretreatment Acid Hydrolysis 1. To 2 ml urine sample, add µl concentrated hydrochloric acid. 2. Heat at 9 C for 2 hours. 3. Add 2 ml of 2 mm Sodium acetate buffer (ph 4.). 4. Add 1 ml of 6 N Potassium hydroxide (KOH). Vortex.. Centrifuge for -6 minutes at,-, rpm (2-22 C). 6. Verify ph of sample is between 4. 6.. 7. Load pretreated sample directly onto Strata-X-Drug B sorbent (see procedure below). Urine: Enzymatic Hydrolysis 1. Dilute µl urine with 1 µl buffer* and 2 µl of 1,, units/ml β-glucuronidase in a 96-well collection plate. 2. Vortex for -6 seconds. 3. Incubate in a water bath at 62 C for 3 minutes. 4. If performing SPE, transfer sample to a 96-well collection plate or autosampler vial, seal and centrifuge for 1 minutes at 2, rpm. Load supernatant onto Strata-X- Drug B sorbent (see procedure below.) If performing rapid removal of β-glucuronidase, load sample directly onto Impact Protein Precipitation plate (see procedure below). Serum / Plasma / Saliva: 1. Dilute plasma/serum 3:1 with 1 % Acetic acid. 2. Vortex. 3. Centrifuge for -6 minutes at 3,-3, rpm (2-22 C). 4. Load pretreated sample directly onto Strata-X- Drug B sorbent (see procedure below). *Buffer prepared by adding 8 ml deionized water and 111 ml glacial acetic acid to a 1 L volumetric flask, adjust final volume to the line with a % KOH solution and mix by inversion. For Ultra Clean and Concentrated Samples Strata-X-Drug B SPE Steps Procedure designed for 6 mg bed mass Strata-X-Drug B and can be adjusted for smaller or larger bed masses. Condition: Not required Equilibrate: Not required Load: Pretreated sample (see above for pretreatment recommendations) Wash: 2 ml of 1 mm Sodium acetate buffer (ph.) Wash 2: 2 ml Methanol Elute: 2 ml Ethyl acetate/isopropanol/ammonium hydroxide (ph.) (7:2:1) Evaporate: to dryness under a stream of Nitrogen at C Reconstitute: 1 ml 1 % Methanol Inject: μl Reduce solvent consumption; no conditioning or equilibration required! For Rapid Removal of Proteins (Ex. β-glucuronidase) Impact Protein Precipitation (PPT) Steps 1. Load 3 µl Acetonitrile to the wells of an Impact Plate 2. Load 1 µl hydrolyzed sample directly onto the Impact Plate 3. Vortex for 2 minutes at maximum possible speed 4. Apply vacuum at 2-7 inches Hg for 2-3 minutes and collect filtrate in a collection plate New long drip format now available! Part no. CE-766

Rapid, Reliable, and High Performance Pain Panel Testing Detection Mode: LC/MS/MS Stationary Phase: Kinetex Core-Shell Biphenyl The new Kinetex Biphenyl ensures that your comprehensive screens display the needed performance with a ruggedness and reliability that will keep you at ease. 22122 2.2e6 2.e6 1.8e6 1.6e6 1.4e6 1.2e6 1.e6 8.e 6.e 4.e 2.e. 2.2e6 2.e6 1.8e6 1.6e6 1.4e6 1.2e6 1.e6 8.e 6.e 4.e 2.e 2212 2.2e6 2.e6 1.8e6 1.6e6 1.4e6 1.2e6 1.e6 8.e 6.e 4.e 2.e 2.6 µm x 2.1 mm 2 bar 1 2 3 4 6 min 2.6 µm 1 2 3 4 6 min µm App ID 22122 x 3. mm 16 bar App ID 2214 x 3. mm 1 bar App ID 2212 Conditions for all separations: Columns: Kinetex 2.6 µm Biphenyl ( x 2.1 mm) Kinetex 2.6 µm Biphenyl ( x 3. mm) Kinetex µm Biphenyl ( x 3. mm) Mobile Phase: A:.1 % Formic Acid in Water B:.1 % Formic Acid in Methanol Gradient: Time (min). 2 4. 4.1..1 7 % B 1 1 2 8 8 8 1 1 Flow Rate:.6 ml/min Injection Volume: 1 µl Column Temperature: 4 C Detection: MS/MS (AB SCIEX API ) Samples: 1. Morphine 2. Oxymorphone 3. Hydromorphone 4. Amphetamine. Methamphetamine 6. MDA 7. Naloxone 8. Codeine 9. 6-MAM 1. Oxycodone 11. MDMA 12. Naltrexone 13. Hydrocodone 14. MDEA 1. Norfentanyl 16. Tramadol 17. Benzoylecgonine 18. Meprobamate 19. Normeperidine 2. Meperidine 21. Norbuprenorphine 22. Fentanyl 23. Buprenorphine 24. Flurazepam 2. PCP 26. Carisoprodol 27. Propoxyphene 28. Midazolam 29. Sufentanil 3. Norpropoxyphene 31. EDDP 32. Lorazepam 33. Methadone 34. Clonazepam 3. Oxazepam 36. Hydroxyalprazolam 37. Nordiazepam 38. Flunitrazepam 39. Temazepam 4. Alprazolam 41. Diazepam 6 1 2 3 4 6 min

Go Even Faster! Using the high performance and versatility of the Kinetex 2.6 µm Biphenyl with a ballistic gradient allows you to cut down even more time from your cycles and significantly improve productivity. Imagine what you can do with this solution on your multiplex or dual-stream systems. 22121.2e6.e6 4.8e6 4.6e6 4.4e6 4.2e6 4.e6 3.8e6 3.6e6 3.4e6 3.2e6 3.e6 2.8e6 2.6e6 2.4e6 2.2e6 2.e6 1.8e6 1.6e6 1.4e6 1.2e6 1.e6 8.e 6.e 4.e 2.e Kinetex 2.6 µm Biphenyl x 3. mm 1 2 3 4 min App ID 22121 Conditions: Column: Kinetex 2.6 µm Biphenyl Dimensions: x 3. mm Part No.: B-4622-Y Guard: SecurityGuard ULTRA Biphenyl Cartridges Part No.: AJ-928 Mobile Phase: A:.1 % Formic Acid in Water B:.1 % Formic Acid in Methanol Gradient: Time (min) % B 1 2. 1 3. 1 3.1 1 1 Flow Rate:.7 ml/min Injection Volume: 1 µl Column Temperature: 4 C Detection: MS/MS (AB SCIEX API ) Analyte Q1 Q3 Retention Time (min) Morphine 286.2 12 1.38 Oxymorphone 32.1 227 1.44 Hydromorphone 286.2 18 1.3 Amphetamine 136.1 91 1.68 Naloxone 328.1 16.2 1.78 Methamphetamine 1.1 91.2 1.84 Codeine 3.1 12.1 1.8 MDA 18.1 133 1.86 Oxycodone 316.2 241 1.92 Naltrexone 342.2 267.1 1.94 Hydrocodone 3.1 199.1 1.96 MDMA 194.1 163.2 1.99 MDEA 28.2 163.2 2.11 Norfentanyl 233.2 84.1 2.2 Tramadol 264.2 8.1 2.24 Benzoylecgonine 29.2 168.1 2.3 Meperidine 248.2 22 2.33 Meprobamate 219.2 18.2 2.3 Norbuprenorphine 414.3 83.2 2.41 Fentanyl 337.2 1.1 2.63 Buprenorphine 468.3.2 2.63 Flurazepam 388.2 31.1 2.7 Analyte Q1 Q3 Retention Time (min) Carisoprodol 261.2 176.2 2.73 PCP 244.3 91 2.74 Propoxyphene 34.2 266.3 2.7 Sufentanil 387.2 238.2 2.77 6-MAM 328.1 16.2 2.8 Midazolam 326.1 291.1 2.83 Normeperidine 234.1 16.2 2.8 EDDP 278.3 234.2 2.9 Methadone 31.2 26.2 2.98 Lorazepam 321 27.1 2.98 Clonazepam 316 27.1 3.3 Norpropoxyphene 38.2 1.1 3. Oxazepam 287.1 241 3. Hydroxyalprazolam 32.1 297 3.12 Nordiazepam 271.1 14 3.12 Flunitrazepam 314.1 268.2 3.21 Temazepam 31.1 2.1 3.24 Alprazolam 39.1 281 3.26 Diazepam 28.1 193.1 3.37 7

41 Pain Management Drugs from Urine in Under. Minutes Detection Mode: LC/MS/MS Stationary Phase: Kinetex Core-Shell Phenyl-Hexyl Achieve excellent resolution and separation of 41 pain management drugs using Kinetex core-shell Phenyl-Hexyl HPLC/UHPLC columns, a great choice for an alternative selectivity as compared to a C18. In addition to being a rapid separation, this method is also available as an imethod Application which allows AB SCIEX mass spec users to download the method and settings directly into their Cliquid software for instant method implementation. 4.6e6 4.2e6 3.8e6 3.4e6 3.e6 2.6e6 2.2e6 1.8e6 1.4e6 1.e6 6.e 2.e. 1 1. 2 2. 3 3. 4 4.. 6 6. min App ID 2847 Column: Kinetex 2.6 µm Phenyl-Hexyl Dimensions: x 4.6 mm Part No.: B-449-E Guard: SecurityGuard ULTRA Phenyl-Hexyl Cartridges Part No.: AJ-8774 Mobile Phase: A: 1 mm Ammonium formate B:.1 % Formic Acid in Methanol Gradient: Time (min) 4.1 7 % B 1 1 Flow Rate:.6 ml/min Injection Volume: 1 µl Column Temperature: 22 C Backpressure: 76.2 bar HPLC System: Agilent 126 Detection: MS/MS (AB SCIEX API ) 2.6 µm Skip the work and instantly run this method using the imethod Kit, a verified method that can be downloaded to your Cliquid software on your AB SCIEX mass spectrometer. imethod Kits contain MRM transitions, LC/MS/MS running conditions, sample preparation recommendations, and all of the consumables you will need to begin running samples. 8 Visit www.phenomenex.com/imethod for more information

Analyte Q1 Q3 Retention Time (min) 6-MAM 1 (quantifier) 328.1 16.2 3. 6-MAM 2 (confirmation) 328.1 211.2 3. Alprazolam 1 39.1 281 4.9 Alprazolam 2 39.1 2.1 4.9 Amphetamine 1 136.1 91. 2.9 Amphetamine 2 136.1 119. 2.9 Benzoylecgonine 1 29.2 168.1 3.6 Benzoylecgonine 2 29.2 1. 3.6 Buprenorphine 1 468.3 396.1 4.3 Buprenorphine 2 468.3 414.2 4.3 Carisoprodol 1 261.2 176.2 4.6 Carisoprodol 2 261.2 97.2 4.6 Clonazepam 1 316. 27.1 4.8 Clonazepam 2 316. 214.2 4.8 Codeine 1 3.1 12.1 2.8 Codeine 2 3.1 11.1 2.8 Diazepam 1 28.1 193.1.2 Diazepam 2 28.1 14..2 EDDP 1 278.3 234.2 4.3 EDDP 2 278.3 186.2 4.3 Fentanyl 1 337.2 1.1 4.3 Fentanyl 2 337.2 188.1 4.3 Flunitrazepam 1 314.1 268.2 4.9 Flunitrazepam 2 314.1 239.2 4.9 Flurazepam 1 388.2 31.1 4.4 Flurazepam 2 388.2 134.1 4.4 Hydrocodone 1 3.1 199.1 3. Hydrocodone 2 3.1 128.1 3. Hydromorphone 1 286.2 18. 2.3 Hydromorphone 2 286.2 128. 2.3 Hydroxyalprazolam 1 32.1 297. 4.8 Hydroxyalprazolam 2 32.1 216.1 4.8 Lorazepam 1 321. 27.1 4.8 Transition 1 for each analyte is used as the quantifier transition. Transition 2 for each analyte is used as the qualifier transition. Achieve Excellent Results, Every Time Recovery, Reproducibility, and Linearity of α-hydroxyalprazolam Concentration Precision Accuracy (ng/ml) (n = 3) 2 2.388 111.4 2 3.384 12.4 Analyte Q1 Q3 Retention Time (min) Lorazepam 2 321. 229.1 4.8 MDA 1 18.1 1. 3.1 MDA 2 18.1 133. 3.1 MDEA 1 28.2 163.2 3.4 MDEA 2 28.2 1.2 3.4 MDMA 1 194.1 163.2 3.2 MDMA 2 194.1 1.2 3.2 Meperidine 1 248.2 22. 3.8 Meperidine 2 248.2 174.1 3.8 Meprobamate 1 219.2 18.2 4. Meprobamate 2 219.2 97.1 4. Methadone 1 31.2 26.2 4.7 Methadone 2 31.2 1. 4.7 Methamphetamine 1 1.1 91.2 3. Methamphetamine 2 1.1 119.2 3. Midazolam 1 326.1 291.1 4.6 Midazolam 2 326.1 249.1 4.6 Morphine 1 286.2 12. 2.1 Morphine 2 286.2 16. 2.1 Naloxone 1 328.1 212. 2.7 Naloxone 2 328.1 23.1 2.7 Naltrexone 1 342.2 267.1 2.9 Naltrexone 2 342.2 212.1 2.9 Norbuprenorphine 1 414.3 11.1 4.1 Norbuprenorphine 2 414.3 16.1 4.1 Nordiazepam 1 271.1 14.. Nordiazepam 2 271.1 16.1. Norfentanyl 1 233.2 84.1 3. Norfentanyl 2 233.2 1. 3. Normeperidine 1 234.1 16.2 3.9 Normeperidine 2 234.1 188.1 3.9 Norpropoxyphene 1 38.2 1.1 4.4 22.7 Norpropoxyphene 2 38.2 143.2 22. 4.4 Analyte area / IS Area 21. 2. 19. 18. 17. 16. 1. 14. 13. 12. 11. 1. 9. 8. 7. 6.. 4. Analyte Q1 Q3 Retention Time (min) Oxazepam 1 287.1 241. 4.8 Oxazepam 2 287.1 269.1 4.8 Oxycodone 1 316.2 241. 3. Oxycodone 2 316.2 26. 3. Oxymorphone 1 32.1 227. 2.2 Oxymorphone 2 32.1 198.1 2.2 PCP 1 244.3 91. 4.2 PCP 2 244.3 19.3 4.2 Propoxyphene 1 34.2 266.3 4. Propoxyphene 2 34.2 91.1 4. Sufentanil 1 387.2 238.2 4. Sufentanil 2 387.2 111. 4. Temazepam 1 31.1 2.1. Temazepam 2 31.1 177.1. Tramadol 1 264.2 8.1 3.7 Tramadol 2 264.2 42.1 3.7 6-MAM-d3 331.1 16. 3. Amphetamine-d 141.1 93. 2.9 Benzoylecgonine-d3 293.2 171.2 3.6 Codeine-d6 36.2 12.1 2.8 Fentanyl-d 342.2 1.1 4.3 Hydrocodone-d6 36.2 22.1 3. Hydromorphone-d6 292.1 18.1 2.3 Meperidine-d4 22.2 224.2 3.8 Methadone-d3 313.2 1. 4.7 Methamphetamine-d 1.2 92. 3. Morphine-d6 292.2 12.1 2.1 Nordiazepam-d 276.1 14.. Normeperidine-d4 238.2 164.2 3.9 Norpropoxyphene-d 313.2 1.1 4. PCP-d 249.3 96. 4.2 3. Recovery, 2. Reproducibility, and Linearity of Methamphetamine 1.. Concentration Precision Accuracy (ng/ml) (n = 3) 12 Analyte Conc./IS Conc. 2.474 113. 1 4.489 11.8 1 1 2 2 3 3 4 4 6 6 7 7 8 8 9 9 1 Analyte area / IS Area 22.7 22. 21. 2. 19. 18. 17. 16. 1. 14. 13. 12. 11. 1. 9. 8. 7. 6.. 4. 3. 2. 1.. Analyte area / IS Area 4 2 48 46 44 42 4 38 36 34 32 3 28 26 24 22 2 18 16 14 12 1 8 6 4 2 1 1 2 2 3 3 4 4 6 6 7 7 8 8 9 9 1 Analyte Conc./IS Conc. 2 4 6 8 1 12 14 16 18 2 22 24 26 28 3 32 34 36 38 4 42 44 46 48 Analyte Conc./IS Conc. 4 2 9

41 Pain Management Drugs from Urine in Under 4 Minutes Detection Mode: LC/MS/MS Stationary Phase: Kinetex Core-Shell C18 Rapidly separate 41 pain management drugs, including critical isobars, using Kinetex core-shell C18 HPLC/UHPLC columns. 2.e6 1.9e6 1.8e6 1.7e6 1.6e6 1.e6 1.4e6 1.3e6 1.2e6 1.1e6 1.e6 9.e 8.e 7.e 6.e.e 4.e 3.e 2.e 1.e.. 1. 1. 2. 2. 3. 3. App ID 2689 Column: Dimensions: Part No.: Guard: Part No.: Kinetex 2.6 µm C18 x 3. mm B-4462-Y SecurityGuard ULTRA C18 Cartridges AJ-877 Mobile Phase: A: 1 mm Ammonium formate B:.1 % Formic Acid in Methanol Gradient: Time (min) 3 4 4.1 % B 1 1 Flow Rate:. ml/min Injection Volume: 1 µl Column Temperature: 22 C Detection: MS/MS (AB SCIEX API 4 ) Analyte Q1 Q3 Oxymorphone 32.1 227 Morphine 286.2 12 Hydromorphone 286.2 18 Amphetamine 136.1 91 Methamphetamine 1.1 91.2 Oxycodone 316.2 241 MDA 18.1 133 MDMA 194.1 163.2 Codeine 3.1 12.1 Naltrexone 342.2 267.1 MDEA 28.2 163.2 6-MAM 328.1 16.2 Hydrocodone 3.1 199.1 Benzoylecgonine 29.2 168.1 Norfentanyl 233.2 84.1 Tramadol 264.2 8.1 Naloxone 328.1 212 Normeperidine 234.1 16.2 Meperidine 248.2 22 Meprobamate 219.2 18.2 PCP 244.3 91 Norbuprenorphine 414.3 11.1 EDDP 278.3 234.2 Norpropoxyphene 38.2 1.1 Clonazepam 316 27.1 Flunitrazepam 314.1 268.2 a-hydroxyalprazolam 32.1 297 Carisoprodol 261.2 176.2 Oxazepam 287.1 241 Methadone 31.2 26.2 Lorazepam 321 27.1 Fentanyl 337.2 1.1 Flurazepam 388.2 31.1 Alprazolam 39.1 281 Temazepam 31.1 2.1 Propoxyphene 34.2 266.3 Nordiazepam 271.1 14 Diazepam 28.1 193.1 Midazolam 326.1 291.1 Sufentanil 387.2 238.2 Buprenorphine 468.3 396.1 Resolution of Critical Isobars Codeine/Hydrocodone 2.4e 2.e 1.e. 3.e 2.e 1.e. 1.e6 9.e 8.e 7.e 6.e.e 4.e 3.e 2.e 1.e. 1.79 1.92. 1 1. 2 2. 3 3. 4 4. min Morphine/Hydromorphone/Norhydrocodone 1.39 1.6 1.83. 1 1. 2 2. 3 3. 4 4. min Oxymorphone/Dihydrocodeine/Noroxycodone 1.47 1.76 1.93 Use our simplified SPE procedure on page 4 prior to HPLC/UHPLC analysis.. 1 1. 2 2. 3 3. 4 4. min 1

Benzodiazepines Detection Mode: LC/MS/MS Stationary Phase: Kinetex Core-Shell Biphenyl Accurately and reliably analyze benzodiazepines using Kinetex Biphenyl HPLC /UHPLC. 7.e 6.e.e 4.e 3.e 2.e 1.e 1 2 3 4 6 min App ID 2219 Column: Kinetex 2.6 µm Biphenyl Dimensions: x 3. mm Part No.: B-4622-Y Guard: SecurityGuard ULTRA Biphenyl Cartridges Part No.: AJ-928 Mobile Phase: A:.1 % Formic acid in Water B:.1 % Formic acid in Methanol Gradient: Time (min) % B 1. 1 2 2 4. 8 4.1 8. 8 7 1 Flow Rate:.6 ml/min Injection Volume: 1 µl Column Temperature: 4 C Detection: MS/MS (AB SCIEX API ) Analyte Q1 Q3 Retention Time (min) Alprazolam 1 (quantifier) 39.1 281.1.8 Alprazolam 2 (confirmation) 39.1 2.1.8 Clonazepam 1 316. 27.1.3 Clonazepam 2 316. 214.2.3 Diazepam 1 28.1 193.1 6.1 Diazepam 2 28.1 14. 6.1 Flunitrazepam 1 314.1 268.2.78 Flunitrazepam 2 314.1 239.2.78 Flurazepam 1 388.2 31.1.12 Flurazepam 2 388.2 134.1.12 Hydroxyalprazolam 1 32.1 297..6 Hydroxyalprazolam 2 32.1 216.1.6 Lorazepam 1 321. 27.1.4 Lorazepam 2 321. 229.1.4 Midazolam 1 326.1 291.1.23 Midazolam 2 326.1 249.1.23 Nordiazepam 1 271.1 14..7 Nordiazepam 2 271.1 16.1.7 Oxazepam 1 287.1 241..4 Oxazepam 2 287.1 269.1.4 Temazepam 1 31.1 2.1.8 Temazepam 2 31.1 177.1.8 11

Opiates from Urine Detection Mode: LC/MS/MS Stationary Phase: Kinetex Core-Shell Biphenyl Achieve resolution of critical isobars such as Morphine / Hydromorphone and Codeine / Hydrocodone using Kinetex core-shell Biphenyl HPLC / UHPLC columns. 2.e6 1.e6. 1 2 3 4 6 min App ID 2218 Column: Kinetex 2.6 µm Biphenyl Dimensions: x 3. mm Part No.: B-4622-Y Guard: SecurityGuard ULTRA Biphenyl Cartridges Part No.: AJ-928 Mobile Phase: A:.1 % Formic acid in Water B:.1 % Formic acid in Methanol Gradient: Time (min) % B 1. 1 2 2 4. 8 4.1 8. 8 7 1 Flow Rate:.6 ml/min Injection Volume: 1 µl Column Temperature: 4 C Detection: MS/MS (AB SCIEX API ) Analyte Q1 Q3 Retention Time (min) 6-MAM 1 (quantifier) 328.1 16.2 3.6 6-MAM 2 (confirmation) 328.1 211.2 3.6 Codeine 1 3.1 12.1 3.63 Codeine 2 3.1 11.1 3.63 EDDP 1 278.3 234.2.36 EDDP 2 278.3 186.2.36 Fentanyl 1 337.2 1.1.1 Fentanyl 2 337.2 188.1.1 Hydrocodone 1 3.1 199.1 3.84 Hydrocodone 2 3.1 128.1 3.84 Hydromorphone 1 286.2 18. 2.76 Hydromorphone 2 286.2 128. 2.76 Meperidine 1 248.2 22. 4.48 Meperidine 2 248.2 174.1 4.48 Methadone 1 31.2 26.2.3 Methadone 2 31.2 1..3 Morphine 1 286.2 12. 2.26 Morphine 2 286.2 16. 2.26 Naloxone 1 328.1 212. 3.1 Naloxone 2 328.1 23.1 3.1 Naltrexone 1 342.2 267.1 3.82 Naltrexone 2 342.2 212.1 3.82 Norfentanyl 1 233.2 84.1 4.24 Norfentanyl 2 233.2 1. 4.24 Normeperidine 1 234.1 16.2 4.47 Normeperidine 2 234.1 188.1 4.47 Norpropoxyphene 1 38.2 1.1.33 Norpropoxyphene 2 38.2 143.2.33 Oxycodone 1 316.2 241. 3.76 Oxycodone 2 316.2 26. 3.76 Oxymorphone 1 32.1 227. 2.44 Oxymorphone 2 32.1 198.1 2.44 Propoxyphene 1 34.2 266.3.2 Propoxyphene 2 34.2 91.1.2 Tramadol 1 264.2 8.1 4.32 Tramadol 2 264.2 42.1 4.32 12

Miscellaneous Drugs of Abuse Detection Mode: LC/MS/MS Stationary Phase: Kinetex Core-Shell Biphenyl Separate various drugs of abuse including amphetamines, cocaine metabolites, and more. 1.2e6 1.e6 8.e 6.e 4.e 2.e 1 2 3 4 6 min App ID 2211 Column: Kinetex 2.6 µm Biphenyl Dimensions: x 3. mm Part No.: B-4622-Y Guard: SecurityGuard ULTRA Biphenyl Cartridges Part No.: AJ-928 Mobile Phase: A:.1 % Formic acid in Water B:.1 % Formic acid in Methanol Gradient: Time (min) % B 1. 1 2 2 4. 8 4.1 8. 8 7 1 Flow Rate:.6 ml/min Injection Volume: 1 µl Temperature: 4 C Detection: MS/MS (AB SCIEX API ) Analayte Q1 Q3 Retention Time (min) Amphetamine 1 (quantifier) 136.1 91. 2.88 Amphetamine 2 (confirmation) 136.1 119. 2.88 Benzoylecgonine 1 29.2 168.1 4.37 Benzoylecgonine 2 29.2 1. 4.37 Carisoprodol 1 261.2 176.2.17 Carisoprodol 2 261.2 97.2.17 MDA 1 18.1 1. 3.49 MDA 2 18.1 133. 3.49 MDEA 1 28.2 163.2 4.6 MDEA 2 28.2 1.2 4.6 MDMA 1 194.1 163.2 3.81 MDMA 2 194.1 1.2 3.81 Meprobamate 1 219.2 18.2 4.44 Meprobamate 2 219.2 97.1 4.44 Methamphetamine 1 1.1 91.2 3.4 Methamphetamine 2 1.1 119.2 3.4 PCP 1 244.3 91..17 PCP 2 244.3 19.3.17 Sufentanil 1 387.2 238.2.2 Sufentanil 2 387.2 111..2 13

Strata-X-Drug B SPE Finally, an SPE Sorbent for Drugs of Abuse Strata-X-Drug B is a polymeric strong cation-exchange SPE sorbent that is designed and quality controlled for basic drugs of abuse analysis. Save time and solvent; no conditioning or equilibrating required Achieve consistent, high recoveries; the sorbent is QC tested using drug probes from urine to ensure batch-to-batch reproducibility Reduce technician training requirements; use a single method to extract multiple drug classes Ordering Information Format Sorbent Mass Part Number Unit Price Tube 1 mg 8B-S128-AAK 1 ml (1/box) 3 mg 8B-S128-TAK 1 ml (1/box) 3 mg 8L-S128-TAK 1 ml (1/box) 3 mg 8B-S128-TBJ 3 ml (/box) 6 mg 8B-S128-UBJ 3 ml (/box) 6 mg 8B-S128-UCH 6 ml (3/box) 6 mg 8B-S128-UCL 6 ml (2/box) 96-Well Plate 1 mg 8E-S128-AGB 2 Plates/box 3 mg 8E-S128-TGB 2 Plates/box 6 mg 8E-S128-UGB 2 Plates/box Tab-less tube Accessories for Tubes Tabless Tube Holders Part No. Description Unit Price AH-94 96-Well 1 ml Tabless Tube Holder for use with 96-well plate ea vacuum manifold (AH-89) AH-9 96-Well 1 ml Tabless Tube Holder for use with positive pressure manifolds ea Manifolds Part No. Description Unit Price AH-623 SPE 12-Position Vacuum Manifold Set, complete assembly ea AH-624 SPE 24-Position Vacuum Manifold Set, complete assembly ea Create a Customized SPE Method in Under 1 Minute www.phenomenex.com/mdtool Accessories for 96-Well Plates Manifold Part No. Description Unit Price AH-89 96-Well Plate Manifold, Universal w/vacuum gauge ea Collection Plates (deep well, polypropylene) Part No. Description Unit Price AH-7192 96-Well Collection Plate, 3 µl/conical /pk AH-7193 96-Well Collection Plate, 1 ml/conical /pk AH-7279 96-Well Collectin Plate, 1 ml Round, 7 mm /pk AH-863 96-Well Collection Plate, 2 ml Square/Round-Conical /pk AH-8636 96-Well Collection Plate, 2 ml Round/Round, 8 mm /pk Sealing Mats Part No. Description Unit Price AH-897 Sealing Mats, Pierceable, 96-Square Well, Silicone /pk AH-8631 Sealing Mats, Pierceable, 96-Round Well 7 mm, Silicone /pk AH-8633 Sealing Mats, Pierceable, 96-Round Well 8 mm, Silicone /pk AH-7362 Sealing Tape Pad 1/pk If Strata-X SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 4 days and keep the Strata-X SPE product for FREE. 14 Phenomenex l WEB: www.phenomenex.com

Ordering Information Core-Shell Technology μm Minibore Columns (mm) SecurityGuard ULTRA Cartridges Phases 3 x 2.1 x 2.1 1 x 2.1 1 x 2.1 3/pk Biphenyl A-4627-AN B-4627-AN D-4627-AN AJ-929 C18 A-461-AN B-461-AN D-461-AN F-461-AN AJ-8782 Phenyl-Hexyl B-463-AN D-463-AN AJ-8788 for 2.1 mm ID μm MidBore Columns (mm) SecurityGuard ULTRA Cartridges Phases x 3. 1 x 3. 1 x 3. 3/pk Biphenyl B-4627-Y D-4627-Y F-4627-Y AJ-928 C18 B-461-Y D-461-Y F-461-Y AJ-877 Phenyl-Hexyl B-463-Y D-463-Y AJ-8781 for 3. mm ID μm Analytical Columns (mm) SecurityGuard ULTRA Cartridges Phases x 4.6 1 x 4.6 1 x 4.6 2 x 4.6 3/pk Biphenyl B-4627-E D-4627-E F-4627-E G-4627-E AJ-927 C18 B-461-E D-461-E F-461-E G-461-E AJ-8768 Phenyl-Hexyl B-463-E D-463-E F-463-E G-463-E AJ-8774 for 4.6 mm ID 2.6 μm Minibore Columns (mm) SecurityGuard ULTRA Cartridges Phases 3 x 2.1 x 2.1 7 x 2.1 1 x 2.1 1 x 2.1 3/pk Biphenyl A-4622-AN B-4622-AN D-4622-AN F-4622-AN AJ-929 C18 A-4462-AN B-4462-AN C-4462-AN D-4462-AN F-4462-AN AJ-8782 Phenyl-Hexyl A-449-AN B-449-AN C-449-AN D-449-AN F-449-AN AJ-8788 for 2.1 mm ID 2.6 μm MidBore Columns (mm) SecurityGuard ULTRA Cartridges Phases 3 x 3. x 3. 7 x 3. 1 x 3. 1 x 3. 3/pk Biphenyl B-4622-Y D-4622-Y F-4622-Y AJ-928 C18 A-4462-Y B-4462-Y C-4462-Y D-4462-Y F-4462-Y AJ-877 Phenyl-Hexyl D-449-Y F-449-Y AJ-8781 for 3. mm ID 2.6 μm Analytical Columns (mm) SecurityGuard ULTRA Cartridges Phases 3 x 4.6 x 4.6 7 x 4.6 1 x 4.6 1 x 4.6 3/pk Biphenyl B-4622-E D-4622-E F-4622-E AJ-927 C18 A-4462-E B-4462-E C-4462-E D-4462-E F-4462-E AJ-8768 Phenyl-Hexyl B-449-E C-449-E D-449-E F-449-E AJ-8774 for 4.6 mm ID 1.7 μm Minibore Columns (mm) SecurityGuard ULTRA Cartridges Phases 3 x 2.1 x 2.1 1 x 2.1 1 x 2.1 3/pk Biphenyl B-4628-AN D-4628-AN F-4628-AN AJ-929 C18 A-447-AN B-447-AN D-447-AN F-447-AN AJ-8782 Phenyl-Hexyl B-4-AN D-4-AN F-4-AN AJ-8788 for 2.1 mm ID 1.7 μm MidBore Columns (mm) SecurityGuard ULTRA Cartridges Phases x 3. 1 x 3. 3/pk C18 B-447-Y D-447-Y AJ-877 for 3. mm ID 1.3 μm Minibore Columns (mm) Phases 3 x 2.1 x 2.1 C18 A-41-AN B-41-AN SecurityGuard ULTRA cartridges require holder, Part No.: AJ-9 If Phenomenex products in this brochure do not provide at least an equivalent separation as compared to other products of the same phase and dimensions, send in your comparative data within 4 days and keep the product for FREE. Phenomenex l WEB: www.phenomenex.com 1

Access Clinical Research Guides, Applications, and More at www.phenomenex.com/clinicaldocs Australia t: 2-9428-6444 f: 2-9428-644 auinfo@phenomenex.com Austria t: 1-319-131 f: 1-319-13 anfrage@phenomenex.com Belgium t: 2 3 41 (French) t: 2 11 8666 (Dutch) f: +31 ()3-2383749 beinfo@phenomenex.com Canada t: (8) 43-3681 f: (31) 328-7768 info@phenomenex.com Denmark t: 4824 848 f: +4 481 626 nordicinfo@phenomenex.com Finland t: 9 4789 63 f: +4 481 626 nordicinfo@phenomenex.com France t: 1 3 9 21 1 f: 1 3 9 21 11 franceinfo@phenomenex.com Germany t: 621-883- f: 621-883-11 anfrage@phenomenex.com India t: 4-312 24 f: 4-312 2411 indiainfo@phenomenex.com Ireland t: 1 247 4 f: +44 162-1796 eireinfo@phenomenex.com Italy t: 1 632711 f: 1 6327 italiainfo@phenomenex.com Luxembourg t: +31 ()3-24187 f: +31 ()3-2383749 nlinfo@phenomenex.com Mexico t: 1-8-844-226 f: 1-31-328-7768 tecnicomx@phenomenex.com The Netherlands t: 3-24187 f: 3-2383749 nlinfo@phenomenex.com New Zealand t: 9-47891 f: 9-47892 nzinfo@phenomenex.com Norway t: 81 2 f: +4 481 626 nordicinfo@phenomenex.com Puerto Rico t: (8) 41-HPLC f: (31) 328-7768 info@phenomenex.com Sweden t: 8 611 69 f: +4 481 626 nordicinfo@phenomenex.com United Kingdom t: 162-1367 f: 162-1796 ukinfo@phenomenex.com United States t: (31) 212- f: (31) 328-7768 info@phenomenex.com All other countries: Corporate Office USA t: (31) 212- f: (31) 328-7768 info@phenomenex.com www.phenomenex.com Phenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at international@phenomenex.com BR18141213_I Terms and Conditions Subject to Phenomenex Standard Terms and Conditions, which may be viewed at www.phenomenex.com/termsandconditions. Trademarks Kinetex is a registered trademark and Strata-X, Impact, MidBore, and SecurityGuard are trademarks of Phenomenex. Agilent is a registered trademark of Agilent Technologies, Inc. Cliquid is a registered trademark and API 4, API, and imethod are trademarks of AB SCIEX Pte. Ltd. AB SCIEX is being used under license. Disclaimers The products mentioned are not intended for clinical use. Because they are not intended for clinical use, no claim or representation is made or intended for their clinical use (including, but not limited to diagnostic, prognostic, therapeutic or blood banking). It is the user s responsibility to validate the performance of Phenomenex products for any particular use, since the performance characteristics are not established. Phenomenex products may be used in clinical diagnostic laboratory systems after the laboratory has validated their complete system as required by the Clinical Laboratory Improvements Amendments of 1988 (CLIA 88) regulation in the U.S. or equivalent in other countries. Strata-X is patented by Phenomenex. U.S. Patent No. 7,119,14 214 Phenomenex, Inc. All rights reserved.