Alternative co-products streams from grains used in pet food applications. Presented by: Greg Aldrich, PhD

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Alternative co-products streams from grains used in pet food applications Presented by: Greg Aldrich, PhD Pet Food R&D Showcase 11Oct2017

Overview Going with the Grain Pet Food Program at K- State Sorghum Fractions Next-Generation Distillers Grains Beyond Grain-Free

Evolution of Premiumization in Pet Specialty over the Past 5 years Lange, 2017 GfK: Petfood Forum 2017

Dog and Cat Food Ingredient Database 44% dog and 47% of cat recipes do not contain grain 28% of dog and 22% of cat have grain free in the product name Dog and Cat Food Ingredient Center http://www.pet-ingredients.com/

But. 80% of the $30 B pet food market remains conventional ingredients Value Premium products are growing As any product position begins to dominate new approaches will be begin to emerge In 1997 there were only a few therapeutic elimination diets - today they seem to predominate. Perhaps a new era is soon to emerge.beyond Grain Free

KSU:Pet Food Program Established KSU 2012 Home: Grain Science & Industry Feed Science & Mgt Training: Short courses, Minor, BS, MS, PhD Cross-campus Initiative ECO-DEVO support

Hal Ross Flour Mill & BIVAP Extrusion Lab

Milling yields (%) for Sorghum used to produce experimental pet diets Item Flour Mill-feed Germ Loss Milled fractions, % 68.3 27.2 1.25 3.25 Proximate analysis of red sorghum (as is) used to incorporate into the dietary treatments Item Whole sorghum Flour Mill-feed Moisture, % 12.94 12.37 11.49 Crude Protein, % 10.50 9.68 13.40 Crude Fat, % 2.81 3.68 5.02 Crude Fiber, % 1.18 n.d. 3.56 Ash, % 1.38 1.19 2.04

Carbohydrate analysis of red sorghum used to incorporate into the dietary treatments Item Whole Sorghum Sorghum Flour Sorghum Mill-feed Crude Fiber, % 1.18 n.d. 3.56 ADF, % 3.80 1.40 7.70 NDF, % 6.70 1.50 16.80 TDF, % 8.80 3.20 20.0 Soluble Fiber, % 2.60 2.50 1.60 Insoluble Fiber, % 6.20 0.70 18.30 Lignin, % n.d. n.d. 2.90 Starch, % 61.5 67.0 43.8

Mean piece mass, volume, density and sectional expansion index (SEI) of kibbles from CON, WSD, FLD and MFD. 1.2 1 0.8 0.6 0.4 a a a b a a a b b b c a 6 5 4 3 2 b c a d CON WSD FLD 0.2 0 P. mass, g P. volume, cm3 P. density, g/cm3 1 0 SEI MFD

Food intake and feces collected (on DM basis) per day, number of defecations per day and fecal scores of dogs fed control (CON), whole sorghum (WSD), flour (FLD) diets and mill-feed (MFD) diets (N=12) Item CON WSD FLD MFD SEM P Food intake, g/d 185 186 181 195 6.5 0.4818 Feces excreted, g/d 42.0 c 55.7 b 32.6 c 95.4 a 3.24 <.0001 Defecations per day 2.18 b 2.38 b 2.10 b 3.02 a 0.098 <.0001 Fecal score 3.60 b 3.68 ab 3.78 ab 3.92 a 0.068 0.0007 abc Means within a row that lack a common superscript differ (P 0.05).

Apparent total tract digestibility determined by estimates of fecal output by chromic oxide of dogs fed control (CON), whole sorghum (WSD), flour (FLD) and mill feed (MFD) diets Item CON WSD FLD MFD SEM P Dry Matter, % 83.0 b 81.1 c 86.0 a 65.9 d 0.44 <.0001 Organic Matter, % 88.0 b 85.8 b 90.7 a 70.6 c 0.34 <.0001 Energy, % 87.2 b 85.4 b 90.3 a 70.2 c 0.68 <.0001 Crude Protein, % 77.5 b 76.3 b 81.8 a 67.2 c 0.73 <.0001 Crude Fat, % 91.5 a 88.4 b 91.4 a 77.9 c 0.37 <.0001 abc Means within a row that lack a common superscript differ (P 0.05).

Oxygen radical absorbance capacity (ORAC) of plasma collected from dogs at the end of each period fed diets based on various sorghum fractions b CON WSD ORAC value b b FLD MFD a 0 5000 10000 15000 20000 25000 μm TE/L

USDA Agricultural Marketing Service: Federal State Marketing Improvement Program Reintroducing Kansas Grains to Midwest Pet food Manufacturing and Processing Markets Sorghum Value Added Pet Food Focus Kansas Department of Agriculture Lynne Hinrichsen (KDA), Greg Aldrich (GSI), Brandi Miller (GSI), Kadri Koppel (SAC), Sarah Sexton-Bowser (CSI) Goal: The Reintroducing Kansas Grains to Midwest Pet food Manufacturing and Processing Markets; Sorghum Value Added Pet Food Focus project is a three-phase project that will provide the necessary education and awareness to assist pet food manufactures in reformulating products and retooling processes to include sorghum in pet food and snack formulations.

ParticiPoll Navigate to: http://igp.participoll.com

Sorghum Utilization What factor limits your inclusion of sorghum in formulations? A) Availability/supply chain surety B) Pricing C) Consumer Perception D) Lack of utilization knowledge A B C D 0

Sorghum Sourcing Would you be interested in directly sourcing grains from farmers? A) Yes B) No C) Yes, if the process was facilitated A B C 0

Next-Generation Distillers Grains Higher protein distillers grains Processing evaluation Physical properties (texture) Digestibility in dogs Palatability in dogs and cats

Kibbles Produced from CGM, SBM, NG-DDG

Nutrient Composition of Experimental Ingredients Expressed on an as-is Basis. Item CGM SBM NG-DDG Moisture 10.17 12.33 6.71 Crude Protein 67.1 47.8 50.8 Crude Fat 1.58 1.12 3.90 Crude Fiber n.d. 3.18 4.13 Ash 1.00 6.10 4.26

Experimental Diets Ingredient Composition to Evaluate NG-DDGs Relative to CGM And SBM. Ingredient Name CGM SBM NG-DDG Base Ration 72.71 72.71 72.71 Corn 33.94 33.94 33.94 Chicken Meal, Low Ash 28.85 28.85 28.85 Beet Pulp 4.00 4.00 4.00 Fish Oil 0.14 0.14 0.14 Vitamins and MInerals 1.35 1.35 1.35 Natural AOX, Dry 0.04 0.04 0.04 Corn Gluten Meal 20.5 - - Soybean Meal - 24.75 - NG-DDG Flinthills (cp 49.2) - - 25 Corn Starch 4.50 0.25 - Titanium Dioxide 0.40 0.40 0.40 Chromium Sesquioxide, mw 151.99 0.25 0.25 0.25 Chicken Fat** 5.00 5.00 5.00 Flavor Powder** 1.00 1.00 1.00 Natural AOX, liquid** 0.03 0.03 0.03 Total **surface applied (not administered through extrusion 100.0001 100.0001 100.0001

Defecations, Scores, and Fecal Output of Dogs Fed Vegetable Protein Diets Diet Item CGM* SBM* NG-DDG* p- Value SEM Daily Defecations 2.03 b 2.43 a 2.38 a 0.0299 0.38 Fecal Score** 3.28 b 3.43 ab 3.64 a <.0001 1.37 Dry Fecal Weight 35.91 c 43.25 b 55.65 a <.0001 4.46 abc indicates that within a row, samples with unlike letters were significantly different (p<0.05) *Corn Gluten Meal (CGM), Soybean Meal (SBM), Next Generation-Distillers Dried Grains (NG-DDG) **Feces Were Scored On A 1-5 Scale with 1 = completely liquid and 5 hard, dry Pellets.

The Effect of Vegetable Protein Source on Nutrient Digestibility by Dogs in Extruded Dry Food Kibble Calculated by Total Fecal Collection. Diet Item CGM* SBM* NG- p- DDG* Value SEM Dry Matter 84.25 a 81.07 b 77.27 c <.0001 1.35 Organic Matter 88.09 a 84.53 b 82.25 c <.0001 1.37 Crude Protein 86.90 a 83.36 b 82.92 b <.0001 1.31 Crude Fat 92.15 a 92.09 a 90.54 b <.0001 0.77 Crude Fiber 30.00 a 23.20 a 3.67 b 0.0007 15.73 Ash 37.39 b 42.50 a 38.12 b 0.0449 5.18 abc indicates that within a row, samples with unlike letters were significantly different (p<0.05) *Corn Gluten Meal (CGM), Soybean Meal (SBM), Next Generation-Distillers Dried Grains (NG- DDG)

The effect of vegetable protein source* on palatability assessed by dogs Diet A vs. B n 1 IR of diet A 2 NG-DDG vs. CGM 17 0.365* SBM vs. CGM 13* 0.432 SBM vs. NG-DDG 20 0.454 *p-value is less than 0.05 1 Number of first visits to bowl with diet B can be obtained by 40-n 2 IR of diet A = intake (g) of diet A/total intake (g) of diets A+B IR of diet B can be obtained by 1-IR of diet A 3 Corn Gluten Meal (CGM), Soybean Meal (SBM), Next Generation-Distillers Dried Grains (NG-DDG)

The effect of vegetable protein source* on palatability assessed by cats Diet A vs. B 3 n 1 IR of diet A 2 NG-DDG vs. CGM 22 0.606* SBM vs. CGM 19 0.632* SBM vs. NG-DDG 20 0.456 *p-value is less than 0.05 1 Number of first visits to bowl with diet B can be obtained by 40-n 2 IR of diet A = intake (g) of diet A/total intake (g) of diets A+B IR of diet B can be obtained by 1-IR of diet A 3 Corn Gluten Meal (CGM), Soybean Meal (SBM), Next Generation-Distillers Dried Grains (NG-DDG)

Conclusions Markets evolve and change Ancient, alternative, and grain co-products may have a place in the next generation of pet foods Sorghum fractions created two potential new products for the pet market Sorghum Bran rich in antioxidants and fiber Sorghum Flour for specialty products Next-generation DDG s create a new alternative protein to soybean meal as a value-added and sustainable contribution to pet foods.

Acknowledgments

Events & Short Courses 2017 October 12-14 Pet Food R&D Showcase Going with the Grain KSU Manhattan December 5 Technolgia de Alimentos para Mascotas: Innovacion en Dietas Superpremuim UABC, MX 2018 January 8-12 Pet Food Formulation for Commercial Production KSU (IGP) April 23-25 Pet Food Innovation Workshop & Petfood Forum August 6-10 Pet Food Workshop & Extrusion Workshop