CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY BSL4 FACILITY. Prof. Maria Rita Gismondo

Meeting of Experts - Palais des Nations Geneva 4-8 August 2014 CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY BSL4 FACILITY L.SACCO UNIVERSITY HOSPITAL, MILAN (ITALY) Prof. Maria Rita Gismondo

Meeting of Experts - Palais des Nations Geneva 4-8 August 2014 CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY BSL4 FACILITY L.SACCO UNIVERSITY HOSPITAL, MILAN (ITALY) Prof. Maria Rita Gismondo RESEARCH ACTIVITIES Antibiotic Resistance, Antiviral Resistance, New Tropical Diseases, Multiresistan Mycobacteria etc Results are published in open scientific literature

Meeting of Experts - Palais des Nations Geneva 4-8 August 2014 CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY BSL4 FACILITY L.SACCO UNIVERSITY HOSPITAL, MILAN (ITALY) Prof. Maria Rita Gismondo INTERNATIONAL PARTNERSHIPS AND PROJECTS Capacity Building Gap Analysis Training (E-learning / In-presence) and Sharing Experience Hands-on courses (BSL2; BSL3 and BSL4) tailored to countries local needs Workshops and seminars

Meeting of Experts - Palais des Nations Geneva 4-8 August 2014 CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY INTERNATIONAL PARTNERSHIPS AND PROJECTS ONGOING BSL4 FACILITY L.SACCO UNIVERSITY HOSPITAL, MILAN (ITALY) Prof. Maria Rita Gismondo 1.The Master Euro-Africain: Biosécurité et Biosûreté 2.Biorisk Assessment Mission, Tiblisi 3.CBRN CoE Initiative (P3, P6, P7, P13 and P18) 4.Instrument for Stability

Meeting of Experts - Palais des Nations Geneva 4-8 August 2014 CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY BSL4 FACILITY L.SACCO UNIVERSITY HOSPITAL, MILAN (ITALY) Prof. Maria Rita Gismondo Develop a holistic and multidisciplinary approach on bio-related issues: bio-threats analysis, biosafety, biosecurity, biorisk assessment and management and biohazardous waste containment Support and foster a risk mitigation culture Support the harmonization of international bio-safety and bio-security standards among the participating countries Strengthen regional and international collaboration and cooperation so as knowledge sharing and lessons learned exchange Enhance networking and awareness raising on bio-related issues and and support synergies among scientific communities and institutions.

Meeting of Experts - Palais des Nations Geneva 4-8 August 2014 CLINICAL MICROBIOLOGY, VIROLOGY AND BIOEMERGENCY BSL4 FACILITY L.SACCO UNIVERSITY HOSPITAL, MILAN (ITALY) Prof. Maria Rita Gismondo Prof. Maria Rita Gismondo, MD mariarita.gismondo@unimi.it Address: Via G. B. Grassi, 74, 20157 Milan (Italy) Phone: +39 02 39042239 / +39 02 50319831 Fax: +39 02 50319832 Dott. Francesca Cerutti secretary@bioemergency.eu

Meeting of Experts Palais des Nations, Geneva 4-8 August 2014. TRAINING ON FIELD A practical training for the isolation of Bacillus anthracis from environmental samples in high-risk areas Antonio Fasanella Istituto Zooprofilattico Sperimentale di Puglia e Basilicata Foggia, Italy

NETWORK OF ISTITUTI ZOOPROFILATTICI SPERIMENTALI

Control of zoonosis Food security programs Management of emergency ( brucellosis, tuberculosis, avian flu, anthrax, blue tongue ) Research on animal infectious diseases Development of vaccines for veterinary use Cooperation with national and international scientific organizations

Anthrax Reference Institute of Italy

Major tasks of the Anthrax Reference Institute of Italy Anthrax vaccine production Molecular epidemiology and genotype database Disease control Bioterrorism emergency ( Italian Laboratory Reference) Animal model Research

Anthrax in Italy In Italy, anthrax is a sporadic disease. Every year, few outbreaks occur in the known risk areas of central-southern Italy and in the major islands. The low diversity of B. anthracis genotypes in Italy suggests a single, dominant historical introduction, followed by localized differentiation

Pollino National Park ( Basilicata Region South Italy) epidemic map, with the representation of the single outbreaks identified by GIS information. Foggia Epidemic outbreak of 2004 During the 2004 summer in the Pollino National Park in Basilicata it was registered an anthrax epidemic that involved 41 farms of 13 towns in an area of about 900 Km 2 in which there were about 7.000 cattle and 33.000 sheep and goats. In 4 weeks 81 cattle died, as well as 15 sheep, 9 goats,11 horses and 8 deer.

Epidemic outbreak of 2004 Bovine Red Deer Horse Carcass burial

Epidemic outbreak of 2004 More than 17% of the population of deer in the park died during the outbreak of anthrax (Fasanella A. et al. 2007 Anthrax in Red Deer (Cervus elaphus), Italy. Emerg Infect Dis. 13(7): 1118 1119)

Epidemic outbreak of 2004 Two human suspect cases

Outdoor laboratory In some restricted areas of the park, for several time were buried animals died of anthrax Vaccination programs ensure the control of the disease in animals that graze in that area. These areas represent a valuable environment for the study of the ecology of Bacillus anthracis

Outdoor laboratory Since 2010, the Anthrax Reference Institute of Italy use this outdoor laboratory for trainings direct to researchers (from foreign countries) regarding the technique of isolation of spores from contaminated environments. The training on field was included in the EU funded project EQADeBa, as an educational activity. These training activities have been continued in the framework of the subsequent Joint Action QUANDHIP. For further information on QUANDHIP, please, see website on next slide.

http://www.quandhip.eu/

First day: Training on field: Program Arrival at the airport of Bari and departure to Pollino National Park. Overnight Second day: Sampling of soils in areas of National Park. Return to Foggia and overnight Third day: Processing of collected samples at the BL3 laboratory of the National Reference Centre for anthrax. Isolation of Bacillus anthracis using GABRI or WHO method Fourth day: Biomolecular analysis of suspect colonies and final meeting Fifth day: Departure from Bari airport

Training on field Processing of collected samples

Training on field Biomolecular analysis of suspect colonies: reading of plates

List of Institutes that participated in the training 1. Österreichische Agentur für Gesundheit und Ernährungssicherheit (AGES) Austria 2. Friedrich-Loeffler-Institut / Bundesforschungsinstitut für Tiergesundheit (FLI) Germany 3. FoHM: Folkhälsomyndigheten Public Healt Agency of Sweden Sweden 4. Public Healt England England 5. Polish National Institute of Hygiene (PZH) Poland 6. Infectiology Centre of Latvia (LIC) Lithuania 7. Federal Office for Civil Protection (FOCP), Spiez Laboratory Switzerland 8. Robert Koch Institute Germany 9. Basurto University Hospital, Bilbao Spain 10. ANSES France 11. Faculty of Veterinary Medicine of Tirana Albania 12. Faculty of Medicine, Mu tah University, Al-karak Jordan 13. Biotechnology Institute of a National Academy of Sciences of the Kyrgyz Republic - Kyrgyzstan

Positive contribution Many researchers have the opportunity to observe B. anthracis in its natural environment Exchange of opinions and experiences Incentive to improve the technique of isolation Start of new collaborations The aim of the training is to prepare the researchers in the techniques of collection of contaminated soil samples. This training aims to verify the contamination level of environment (natural or with suspect of a deliberate release). The training is free and is directed to researchers prior approval of the Italian government authorities

Contacts: Antonio FASANELLA a.fasanella@izsfg.it Angela ACETI acetiangela@hotmail.com Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata WEB SITE: www.izsfg.it