Ceramic Veneers: A Step-by-Step Case Report

Similar documents
Porcelain veneers: Treatment guidelines for optimal aesthetics

Anterior Esthetic Techniques & Materials

A conservative restorative smile makeover

COURSE CURRICULUM FOR AESTHETIC DENTISTRY

Shadeguides Ceramic Veneers: Tooth Preparation for Enamel Preservation

No Prep And Minimal Prep Veneers

Restoration of the worn dentition

SCD Case Study. Background

Minimally invasive veneers

ESTHETIC REHABILITATION OF PATIENT USING PORCELAIN VENEERS: A SERIES OF 3 CASE REPORTS

JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH

Esthetic Rehabilitation of Severely Discolored Maxillary Anterior Teeth with Porcelain Laminate Veneers: A Case Report

A clinical case involving severe erosion of the maxillary anterior teeth restored with direct composite resin restorations

The use of Ceramic Veneers to esthetically rehabilitate a dentition with Severe Fluorosis: A Case Report

Hands-on Posterior Tooth Preparation. Practical Skills Courses, SWL, 25/11/2016

Composite Veneers Vs Porcelain Veneers Which one to choose?

Creating deluxe aesthetics with direct, layered composite resin veneers

Complex esthetic and functional rehabilitation using glass-ceramic materials - long-term documentation of a restoration

The Use of Pre-Fabricated Composite Veneers to Enhance Esthetics

Esthetic Correction with Laminate Veneers - A Case Series

Ceramic Based Aesthetic CAD/CAM Restorative

Contraindicated internal bleaching what to do?

Lingual Veneers, a conservative approach

Introduction to Layering with Filtek Supreme Plus Universal Restorative. Filtek. Supreme Plus Universal Restorative

Optimizing Esthetics with Ceramic Veneers: A Case Report

MAURO FRADEANI, MD, DDS

Simple. Esthetic. Efficient. Available exclusively from:

Two approaches, one goal: Digital expertise versus manual skill in the fabrication of ceramic veneers

CERASMART. The new leader in hybrid ceramic blocks

Digital Smile Design using the M Proportions and GPS 2D to 3D Digital Facebow: Clinical Case 1

5,6 Significant improvements of the dentin bond

ADHESIVE RECONSTRUCTION IN HELP OF THE ORTHODONTIC TREATMENT

Smile design with composites: A case study

For many years, patients with

Practicing Minimally Invasive Dentistry with Durability and Esthetic in Mind

The power of four: Aesthetic treatment in the anterior area.

CPR for Complex Dental Treatment; From Concept, to Prototype, to Restoration

Calibra. Cements. The Simple Choice for Easy Cleanup

FRACTURE RESISTANCE OF FELDSPATHIC VENEERS WITH DIFFERENT PREPARATION DESIGNS IN VITRO

how to technique Issue How to improve the longevity of porcelain veneers.

6/1/15. Bonding Ceramic Veneers in What we did? What we do? Using Evidence-Based Dentistry XXXX ?????

Smile Designing with Ceramic Veneers and Crowns

Based on their strength, longevity, conservative nature,

speaker Dr. Mauro FRADEANI from JUNE 28 to JULY The esthetic rehabilitation FULL IMMERSION PROGRAM A COMPRENSIVE PROSTHETIC APPROACH

Invasive lumineer prosthodontic correction of malaligned teeth

MINIMAL INTERVENTION DENTISTRY THE PENN COMPOSITE STENT

A Step-by-Step Approach to

Restoration of teeth using lithium disilicate glass-ceramics in a patient with Dentinogenesis Imperfecta

Lect. 3 operative Dr. Ameer AL-Ameedee

illustrated technique guide

Management of Inadequate Margins and Gingival Recession Presenting as Tooth Sensitivity

TECHNICAL GUIDE. For use with CEREC

Patient demand for esthetic dentistry

Jay M. Lerner, DDS* Key Words: anterior, conservative, preparation, minimally invasive, porcelain, porcelain laminate veneers (PLVs)

Porcelain veneers: techniques and precautions

GENERAL DENTISTRY & COMPREHENSIVE CARE

A Systematic Approach to Anterior Esthetics

Restorations with CAD/CAM technology

SMILE DESIGN SEVEN KEY AREAS

Esthetic rehabilitation of crowded maxillary anterior teeth utilizing ceramic veneers: a case report Süha Türkaslan* and Kivanç Utku Ulusoy

ANTERIOR ESTHETIC RESTORATIONS USING DIRECT COMPOSITE RESTORATION AND ALL CERAMIC VENEERS - 2 CASE REPORTS

Direct composite restorations for large posterior cavities extended range of applications for high-performance materials

Predictable Real World Aesthetics. The Key to Success with Natural Restorations

Minimally Invasive Porcelain Laminate Veneer Preparation Design

Active Clinical Treatment Case 48

PREDICTABILITY IN COMPREHENSIVE RECONSTRUCTION Bite registration and recovery process for comprehensive reconstructive cases.

16 Vol. 1, No _CEREC_Griffin.qxd 7/25/06 5:48 PM Page 16

allinone... unbelievable? But true! Picture: Dr. Thano Kristallis

JMSCR Vol 05 Issue 01 Page January 2017

5Recommended Shade-Matching Protocol

Chairside Reference Guide

MINIMAL PREPARATION VENEER CASE SELECTION PROCESS

Midline Diastema Closure Following Post-Orthodontic Treatment Relapse Using Metal Free Restorations

Posterior Adhesive Dentistry

Prefabricated Composite Resin Veneers A Clinical Review

Aesthetic Closure Of Maxillary And Mandibular Anterior Spaces Using Direct Composite Resin Build-Ups: A Case Report

Direct restoration in the aesthetic zone - a case study

Empress * CAD IPS. Confidence. Reliability. Esthetics. IPS Empress CAD for the CAD/CAM Technology Information for Dentists

The mock-up: your everyday tool

CAD/CAM PREPARATION GUIDELINES & TISSUE MANAGEMENT TECHNIQUES RECOMMENDATIONS FOR OPTIMAL SCANNING, DESIGNING, AND MILLING

Nine Steps To Occlusal Harmony

Individual Tools for Controlling and Positioning Porcelain Veneers: Easychecker The Aesthetic Key Ralf Barsties, CDT and Dr.

All too often, restorative dentists

A conservative smile makeover utilizing both a minimally invasive and non-invasive prep-less porcelain veneer technique.

Smile creation for misaligned dentition

Psychological Impact of Communication

Simultaneous implant reconstruction of the maxilla and mandible

Awareness of the population about ceramic laminate veneers in Saudi Arabia

Dental erosion is spreading rapidly among

Case Report - Dr. Arthur Weiss

Contouring vs. Orthodontics. Contouring to Eliminate Fractures and Enhance Proportions

Empress Esthetic Special Edition

Used Products. Variolink N LC. Proxyt fluoride-free. OptraStick. Ivoclean. Monobond N. OptraDam. N-Etch. Tetric N-Bond.

Anew system for placing direct composite resin veneers, the

2017 Oregon Dental Conference Course Handout

Peninsula Dental Social Enterprise (PDSE)

Minimally invasive vertical preparation design for ceramic veneers

Bonding to dentine: How it works. The future of restorative dentistry

STAGES CORE RESTORATIONS FOUNDATION WORK 09/03/2018. Endodontic treatment. Extractions. Crown lengthening. Implants.

Types of prostetic appliances Dr. Barbara Kispélyi

Transcription:

20 Global Journal of Oral Science, 2016, 2, 20-27 Ceramic Veneers: A Step-by-Step Case Report Manuele Mancini 1,* and Maurizio Mancini 2 1 Department of Dental Materials, University of Rome Tor Vergata, Rome, Italy and 2 Private practice, Rome, Italy Abstract: The success of aesthetic rehabilitation never depends on clinical procedures only. A proper dental technique is required as well as the respect for some biomimetic principles to obtain the aesthetic final result. This case report aimed to describe a prosthetic rehabilitation with all-ceramic veneers of a patient unsatisfied of her smile. A 60-year-old female patient was reported to our clinic complaining about the lifelike appearance of her composite restorations. Before restoring the upper frontal teeth, a mock-up was conducted to verify the patient's satisfaction. A ceramist conducted all the fabrication process so that surface characterizations could be visually verified and the lifelike appearance of natural tooth could be reproduced. After the cementation procedure, the patient reported being satisfied with the lifelike appearance of the new restorations. Based on the clinical findings of the present case report, it can be concluded that the reproduction of the lifelike aesthetic appearance of natural teeth and the visualization of the final results before definitive procedures are essential to obtain the clinical success. Keywords: Ceramic veneers, Aesthetic evaluation, Resin cement, Diagnostic wax-up. 1. INTRODUCTION The patients demand for treatment of unaesthetic anterior teeth is steadily growing. Historically, the most predictable and durable treatment was with the preparation of full coverage crowns with the removal of substantial amounts of tooth structure. However, this approach is undoubtedly most invasive with substantial removal of large amounts of sound tooth substance and possible adverse effects on adjacent pulp and periodontal tissues. Following the introduction of bonding by Buonocore in 1955, research led to the development of multi-step total-etch adhesive systems [1-3], along with the development of high performance and more universally applicable small particle hybrid resin composites has led to more conservative restorative adhesive techniques to deal with unaesthetic tooth appearance. Resin composites can be used to mask tooth discolourations and improve tooth contours and positions. However, such restorations still suffer from a limited longevity, because resin composites remain susceptible to discoloration, wear and marginal fractures, reducing thereby the aesthetic result in the long term [4,5] Due to these concerns, resin composites have been recommended for minor cases and for short to medium term restorations. Charles Pincus [6] introduced porcelain veneers in 1938 to provide temporary aesthetic improvement to patients in the film industry. These were retained with the use of a denture adhesive, but it was with Simonsen and Calamia [7] as well as with Horn [8] that the interest in porcelain veneers * Address correspondence to this author at the Department of Dental Materials, University of Rome Tor Vergata, Rome, Italy; E-mail: manuele.mancini@uniroma2.it was reactivated by introducing special acid etching procedures that substantially improved the long-term porcelain veneer retention. Porcelain veneers have superior aesthetics especially over the longer term. Due to the biocompatibility and non-porous nature of the porcelain, this minimises plaque adherence, with no adverse effect on gingival health in well-maintained mouths. Porcelain veneers have become an important treatment modality in providing solutions to patient s aesthetic and functional problems and need careful planning and execution to ensure a successful longterm result. Laminate veneers came as a good alternative when full veneer crowns were cemented to the teeth after extensive preparation, which put the tooth vitality into jeopardy. Glazed porcelain has a longstanding history as a nonporous, biocompatible and highly esthetic material with high resistance to stain and abrasion. These features along with the acid etching, silane coupling, enamel and dentine bonding agents and the improved composite resins puts the ceramic laminate veneer system a step above the rest. Most importantly the conservative approach towards its preparation will always make a sensible dentist to think before going on to any alternative esthetic procedure. Ceramic veneers can be offered as the treatment option in a wide variety of different cases such as: Abrasion; Coronal fracture; Correcting tooth defects (e.g. the closure of interdental spacing and restoration of malformed teeth where crowns are not indicated); Diastema; 2016 Revotech Press

Ceramic Veneers: A Step-by-Step Case Report Global Journal of Oral Science, 2016, Vol. 2, 21 Orthodontics (e.g. discrepancies in the size and shape of teeth that are not correctable by orthodontics alone); Tooth discoloration (especially for treatment of discoloured teeth that do not respond to toothwhitening or micro-abrasion procedures); To adjust occlusion (e.g. realignment of in-standing, rotated or protruding teeth). The above features mean that, when used appropriately, ceramic veneers can dramatically transform unaesthetic, damaged dentitions in ways once thought impossible. Indeed, their aesthetic and strength characteristics are closer to those of natural tooth structure than almost any other dental restoration. They are not, however, without limitations: More than one appointment is required; Prior to cementation they are fragile and difficult to manipulate; Repair can be difficult; Satisfactory provisional restorations can be difficult to make and retain; Some tooth preparation is usually necessary; Their colour cannot easily be modified once placed; They are more costly than a number of possible alternatives; They are technique-sensitive and time-consuming to place; Veneers are not indicated in heavily restored teeth, worn teeth and any teeth with insufficient enamel available for bonding or teeth too weak to withstand functional forces; There are not predictable results where the spaces requiring closure are too wide to be closed just by increasing tooth width alone; It should be aware veneers is not indicated where any tooth discolouration is too severe to be masked by a thin porcelain veneer and where thickening of the veneer would require extensive preparation into dentin; In non-vital teeth for reasons of tooth weakness and the possibility of subsequent, unfavourable, colour changes, ceramic veneers are not indicated. Before preparing the teeth a complete analysis should be carried out in order to optimize the result. In this way it can be ensured that the teeth being veneered will need only minimal preparation, or in some areas none at all. The teeth being restored with ceramic veneers are prepared between 3 and 30 per cent only, whereas in comparison a tooth being prepared for either a full metal-gold or bonded crown will receive a preparation of between 63 and 72 per cent that is somewhat invasive. Although the literature contains a variety of recommendations with regard to tooth preparation, luting cement and ceramic material [9], the technique almost invariably comprises a thin ceramic laminate veneer bonded, ideally, to a predominantly enamel substrate by means of a composite resin luting cement aided by the application of silane to the etched porcelain fitting surface. 2. CASE REPORT In this paper it will be reported a case where a canine-canine-rehabilitation was performed, following a step-by-step technique using feldspathic porcelain veneers. 2.1. Informed Consent Ceramic veneers are an esthetic procedure and require a full discussion on the benefits and risks with the functional and aesthetic objectives defined within this process. Alternative treatments to achieve the patient s goals must be mentioned and a discussion on the procedures involved with the steps from start to completion. 2.2. Selection of the Shade The shade selection can be challenging due to the thin and translucent nature of the veneer. The final colour is a result of the underlying tooth colour, the luting resin and the opacity/translucency of the porcelain used. The colour of the existing teeth can be shared with the ceramist, using photographs and/or a spectrophotometer (e.g. VITA Easyshade). 2.3. Diagnostic Wax-Up/Mock-Up A clear treatment planning is critical, especially to understand where treatment is heading. The utilisation of a wax-up can assist in the desired aesthetic appearance. This wax-up is also necessary to create the putty keys for temporaries and reduction guides useful during preparation. The contours and form of the final teeth can be transferred from the desired wax up to the

22 Global Journal of Oral Science, 2016, Vol. 2 Mancini and Mancini provisionals allowing patients to have a preview of their desired appearance (Figures 1-4). 2.4. Teeth Preparation The aims of tooth preparation are to: Maintain the preparation within enamel; Provide a finished preparation, which is smooth and free of any sharp internal line-angles, which may cause stress concentration within the ceramic; Provide a margin from which the dental technician can create a normal emergence of the veneer from the gingival margin; Figure 1: Preoperative. Figure 2: Preoperative. Close-up. Provide definite seating landmarks making proper seating of the veneer; Provide enough thickness for the porcelain for sufficient fracture resistance. 2.4.1. Vestibular Preparation The preparation of the buccal plane of the incisors needs to be addressed in three planes with incisal, middle and cervical planes. A careful labial reduction (Figure 5) is carried out to provide a minimum of 0.3 to 0.6 mm, depending on the material chosen for the veneer (feldspathic or lithium disilicate). There are three methods to reduce the vestibular enamel: Figure 3: Diagnostic wax-up. Figure 5: Gingival retraction cord in-situ, #1 cord (Ultrapak, Ultradent). 1. Freehand; 2. Use of depth cuts/grooves. The use of standardised objects allows accurate judgement of depth; Figure 4: Bis-GMA temporary created from the diagnostic wax-up, using a PVS reduction index. 3. Use of silicone putty index. Using a silicone index may help in assessing the amount of tissue reduction and can be prepared by the dentist or the dental ceramist from the initial wax-up model. When viewed from the occlusal view, this can be cut in horizontal slices, which can be peeled back to assess different vertical positions of the reduced teeth. Utilisation of a silicone index derived from the

Ceramic Veneers: A Step-by-Step Case Report Global Journal of Oral Science, 2016, Vol. 2, 23 wax-up allows a visualisation of the reduction required to achieve the form and contours of the preplanned shape and length of the final veneers (Figures 6,7). Figure 6: PVS reduction index in use on the right side. 0.4 mm. This allows the veneer to avoid an overcontour. Additionally, it allows simple seating of the veneer and minimises stresses, enhancing the future fracture resistance unit tooth-veneer. Unlike crown margins that are often buried in the sulcus, the use of the thin translucent porcelain allows often a contact lens effect where the margins are blended with no discernible demarcation. With the supra-gingival preparation there are many advantages, such as: margins will be very often in enamel (extending the longevity of the veneer and not revealing a distinct line of demarcation between the veneer and the natural tooth); there will be less risk of exposing dentine (with less chance of injury to the soft tissues during preparation); impression taking is easier (with no need for retraction) (Figures 8,9). Figure 7: PVS reduction index in use on the left side. 2.4.2. Incisal Edge Reduction Different preparation designs have been advocated from feather and window preparations that involve no reduction of the incisal edge or preparation of the lingual surfaces, to other preparations that involve a reduction of the incisal edges. The author prefers an incisal preparation that is carried over the incisal edge from buccal to palatal, with up to 1.5-2 mm of incisal reduction. According to Calamia [10,11] and Magne [12], a tooth preparation that incorporates incisal edge is preferable, because the veneer is stronger and provides a positive seat during cementation. Moreover, this preparation design is simple and the incisal translucency is easier to be created by the ceramist. 2.4.3. Proximal Preparation Interproximally the clinician should stop the preparation before the contact area if the contour of the tooth does not need to be changed. On the contrary, breaking the contact may be necessary to clear the contact in certain situations, such as diastema. 2.4.4. Cervical Margin The cervical preparation for a veneer is recommended to be a chamfer design with a maximum depth of Figure 8: Preparation on the right side (the presence of infiltrated Class III restoration, previously done, will be treated after the cementation of the veneer). Immediate dentine sealing was performed. Figure 9: Preparation on the left side (The presence of infiltrated Class III restoration, previously done, will be treated after the cementation of the veneer). Immediate dentine sealing was performed. 2.5. Facebow Transfer and Bite Registration To communicate clearly to the dental technician the correct final orientation of the incisal plane of the planned veneers, it is important that they receive a stick bite or symmetry bite. This can be as simple as two sticks within the bite registration to register the midline and the inter-pupillary line to the teeth.

24 Global Journal of Oral Science, 2016, Vol. 2 Mancini and Mancini 2.6. Temporization Some clinicians feel that provisionalisation is not necessary with veneers due to the minimal tooth reduction required. On the contrary, some others think that temporization is an integral part of the treatment process, especially considering that provisionals (if taken from the diagnostic wax-up) give the patient a preview of the final result (Figure 10). Figure 12: Close-up of the palatal margin of veneers. Note the perfect adaptation of the veneer on the Type IV plaster master model. Figure 10: Temporaries in situ. Temp-Bond Clear Dental) was used. (Kerr 2.7. Try-in of Veneers Once the material for the veneer was chosen (feldspathic ceramic or lithium disilicate), and the ceramist has created the veneers (Figures 11-13), the tooth surface should be cleaned of any residual resin cement or provisional material, to ensure perfect adaptation of the veneers, and each veneer should be tried individually to assess fit. This phase can be done with or without water or try-in gels (without the complete seating and marginal adaptation can be better visualised). Incomplete seating is normally due to remaining provisional material, luting resin that has not been removed or tight contact points. Once the patient is happy and has approved the final aesthetics, the restorations are prepared for cementation (Figures 14,15). Figure 13: Type IV plaster master model. Figure 14: Smile with veneers in situ with try-in gel (NX3 Kit, Kerr Dental). Figure 11: Feldspathic ceramic veneers. Figure 15: Veneers in situ with try-in gel.

Ceramic Veneers: A Step-by-Step Case Report Global Journal of Oral Science, 2016, Vol. 2, 25 2.8. Bonding Veneers The inner surface of veneers (being silica-based restorations) must be etched with 9.5% hydrofluoric acid for 20 seconds (for lithium disilicate) or 60 seconds (for other silica based ceramics). The acid should be thoroughly cleansed with air-water spray and the porcelain should then be placed into a container of distilled water (or 95% alcohol or acetone) and put into an ultrasonic bath for 4 minutes to remove any residues remaining on the surface. Restorations are removed, dried and silane primer is applied to the fitting surface, which helps provide a chemical covalent bond to the ceramic. This is allowed to remain on the veneer for 1 minute and after that the veneer should be gently blown with air to evaporate any remaining solvent. The application of rubber dam is mandatory to achieve adequate isolation (Figure 16). Light curing composite resin is preferred for cementation of the veneers as they have a longer working time than dual cure or chemically cured composites (Figure 17). This allows sufficient time to remove excess composite prior to curing and thus reduces the finishing procedures. The use of various coloured resin cements has a 10% influence on the final result obtained (Figure 18). Figure 17: Enamel etched on tooth 1.1. Figure 18: Veneer on tooth 1.1 luted (NX3 cement, Kerr Dental). photocuring Figure 16: Proper rubber dental dam isolation with singletooth etching technique. 2.9. Finishing and Polishing If the bonding procedure was completed smoothly with a well fitting veneer, there should be very little cement to clean up from around the margins (Figure 19). It is preferred not to use a rotary instrument as a #12 blade is able to carefully remove excess cement. Figure 19: Veneers luted on sector I.

26 Global Journal of Oral Science, 2016, Vol. 2 Mancini and Mancini However sometimes a fine diamond bur can be used to adjust the porcelain that will be then carefully polished. Occlusion is carefully checked initially with centric occlusion followed by other excursive movements. The use of rugby-shaped diamonds with water spray can be used to adjust the porcelain. Any adjustments must be further polished (Figure 20-23). The long-term clinical success (Figure 24) of porcelain veneers depends on a careful case selection and diagnostic approach, as well as accurate and appropriate tooth preparation and adhesive bonding procedures. visualization of the final results before definitive procedures are essential to obtain the clinical success. Figure 23: Immediate esthetical result integration with lips Lateral view. Figure 20: All veneers luted. Figure 21: Immediate postoperative. Figure 22: Immediate esthetical result integration with lips Frontal view. 3. CONCLUSION Based on the clinical findings of the present case report, it can be concluded that the reproduction of the lifelike aesthetic appearance of natural teeth and the Figure 24: Pre-op and Post-op. REFERENCES [1] Nakabayashi N, Kojima K, Masuhara E. The promotion of adhesion by infiltration of monomers into tooth substrates. J Biomed Mater Res. 1982; 16: 265-73. http://dx.doi.org/10.1002/jbm.820160307 [2] Van Meerbeek B, Vanherle G, Lambrechts P, Braem M. Dentin- and enamel-bonding agents. Curr Opin Dentistry 1992; 2: 117-27. [3] Pashley DH, Ciucchi B, Sano H, Horner JA. Permeability of dentin to adhesive agents. Quintessence Int. 1993; 24: 618-31. [4] Peumans M, Van Meerbeek B, Lambrechts P, Vanherle G. The five-year clinical performance of direct composite additions to correct tooth form and position. Part I: aesthetic qualities. Clin Oral Investig. 1997; 1: 12-18. http://dx.doi.org/10.1007/s007840050003 [5] Peumans M, Van Meerbeek B, Lambrechts P, Vanherle G. The five-year clinical performance of direct composite additions to correct tooth form and position. Part II: marginal qualities. Clin Oral Investig. 1997; 1: 19-26. http://dx.doi.org/10.1007/s007840050004 [6] Pincus CR. Building mouth personality. J S California Dental Assoc. 1938;14: 125-9. [7] Simonsen RJ, Calamia JR. Tensile bond strength of etched porcelain. J Dental Res. 1983; 62: 297 Abstract 1154.

Ceramic Veneers: A Step-by-Step Case Report Global Journal of Oral Science, 2016, Vol. 2, 27 [8] Horn RH. Porcelain laminate veneers bonded to etched enamel. Dental Clin N America 1983; 27: 671-84. [9] Sadowsky SJ. An overview of treatment considerations for esthetic restorations: a review of the literature. J Prosthet Dentistry 2006; 96: 433-42. http://dx.doi.org/10.1016/j.prosdent.2006.09.018 [10] Calamia JR. The etched porcelain veneer technique. NY State Dent J. 1988: 54: 48-50. [11] Calamia JR, Calamia CS. Porcelain laminate veneers: reasons for 25 years of success. Dental Clin N America 2007; 51(2): 399-417. http://dx.doi.org/10.1016/j.cden.2007.03.008 [12] Magne P, Belser U. Bonded porcelain restorations in the anterior dentition: A Biomimetic Approach. Germany: Quintessence, 2003. Received on 05-11-2015 Accepted on 25-11-2015 Published on 30-01-2016 2016 Mancini and Mancini; Licensee Revotech Press. This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.