FirePlex Multiplex Immunoassay product list Contents Purchasing the assay Human - pre-designed panels Human list of analytes Human protein standard mixes Mouse - pre-designed panels Mouse list of analytes Mouse protein standard mixes Purchasing the assay Getting started with FirePlex immunoassays in your own lab is simple: 1. Select a pre-designed panel or design your own panel from the full list of analytes below. Add any additional analytes you might want to a pre-designed panel 2. Choose one or more protein standard mixes from the list below 3. Add a human (ab208203) or mouse (ab209499) multiplex immunoassay core kit If you d rather discuss your specific needs with one of our expert support scientists or request a quote for services, please contact us. All human analytes are validated for use with serum, plasma (EDTA, heparin, citrate), cell culture supernatant, urine, saliva, milk (defatted), bronchial lavage (BAL), cerebrospinal fluid (CSF) and synovial fluid samples. All mouse analytes are validated for use with serum, plasma (EDTA, heparin, citrate), cell culture supernatant & urine. All sample types should be diluted by at least 1:4 before running the assay. Cell culture supernatant may be used at 1:2 dilution or neat if necessary to improve assay sensitivity although assay linearity is improved at 1:4 dilution.
Pre-designed human panels Panel Plex abid Analytes Key cytokines 15 ab213392 TNF alpha, IL6, IL8, IL10, IL12 p70, IFN gamma, IL4, IL2, IL5, IL9, IL13, IL17A, GM-CSF, MCP1, IL1 beta Th1/Th2/Th17 7 ab213390 IL2, IL4, IL6, IL10, TNF alpha, IFN gamma, IL17A Th1/Th2 7 ab213389 IL2, IL4, IL5, IL6, IL10, TNF alpha, IFN gamma Inflammation 8 ab213391 IL6, IL8, IL10, TNF alpha, IL12 p70, IL1 beta, MCP1, IFN gamma Key cytokines 4 ab213388 TNF alpha, IL6, IFN gamma, IL10 List of human analytes Once you ve assembled your custom panel or added extra analytes to a pre-designed panel, we can pre-mix the separate assay reagents into a single vial to save you time. Just request a quote for this option. In rare cases, combinations of analytes cannot be run together in the same panel, but we ll check this when you place an order or request a quote. Assay dynamic ranges can be extended with an alternate extended standard curve, typically 1.5 3,333 becomes 0.61-10,000 and 4.6 10,000 becomes 1.83 30,000. abid Dynamic Range (pg/ml) low high Limit of Detection (pg/ml) alpha Fetoprotein ab213387 41.2 90,000 6.32 Apolipoprotein AII ab219002 41.15 90,000 17.30 Apolipoprotein A-IV ab219003 1111.11 2,430,000 313.00 BCA1 ab208220 4.6 10,000 0.79 BDNF ab219005 4.57 10,000 2.70 BST2 ab219006 1.52 3,333 0.30 Cardiotrophin 1 ab219007 1.52 3,333 0.22 CD14 ab219008 4.57 10,000 2.0 CD276 ab219009 123.46 270,000 26.34 CD40 ab219010 4.57 10,000 0.95
CD40 Ligand ab213382 1.5 3,333 0.44 CEA ab213386 123.5 270,000 20.20 CEACAM1 ab219011 4.57 10,000 0.90 Coronin 1a ab219012 41.15 90,000 32.50 CRP ab213377 4.6 10,000 0.87 CXCL16 ab213374 4.6 10,000 0.65 CXCL5 ab213384 4.6 10,000 0.65 CXCL7 ab219013 1.52 3,333 0.40 Cystatin C ab219014 13.72 30,000 6.0 EGF ab219015 0.51 1,111 0.07 Eotaxin ab208213 1.5 3,333 0.49 FGFR3 ab219016 41.15 90,000 8.20 Fibrinogen ab213385 123.5 270,000 52.66 Fractalkine ab208223 1.5 3,333 0.29 G-CSF ab208218 4.6 10,000 1.78 GM-CSF ab208216 4.6 10,000 2.13 GRO alpha ab209509 1.5 3,333 0.29 ICAM1 ab213381 13.7 30,000 1.85 ICAM2 ab219017 123.46 270,000 23.09 IFN-gamma ab209504 4.6 10,000 0.67 IGFBP3 ab219018 41.15 90,000 25.40 IL-1 beta ab209502 1.5 3,333 0.46 IL-10 ab208209 4.6 10,000 1.76 IL-12p70 ab209505 1.5 3,333 1.06 IL-13 ab208210 4.6 10,000 2.52 IL-15 ab219019 4.57 10,000 0.86 IL-16 ab213375 13.7 30,000 3.72
IL-17A ab208215 4.6 10,000 2.88 IL-17F ab213380 4.6 10,000 0.98 IL-18 ab219020 13.72 30,000 2.43 IL-1a ab209844 1.5 3,333 1.19 IL-1Ra ab208224 1.5 3,333 0.76 IL-2 ab208207 4.6 10,000 4.05 IL-22 ab219021 1.52 3,333 0.24 IL-23 ab209508 4.6 10,000 1.94 IL-3 ab219022 4.57 10,000 1.22 IL-4 ab208208 4.6 10,000 1.23 IL-5 ab208225 1.5 3,333 0.50 IL-6 ab208206 4.6 10,000 2.46 IL-6 R alpha ab208217 13.7 30,000 4.62 IL-7 ab208226 1.5 3,333 0.74 IL-8 ab208214 1.5 3,333 0.34 IL-9 ab209506 1.5 3,333 0.51 Interferon alpha 1 ab219023 0.51 1,111 0.07 IP-10 ab209503 1.5 3,333 0.2 Leptin ab213379 41.2 90,000 10.70 Lipocalin-2 ab219024 1.52 3,333 0.40 Lipoprotein A ab219025 3333.33 7,290,000 1001.2 LOX1 ab219026 1.52 3,333 0.70 MCP1 ab208211 1.5 3,333 0.44 MCP3 ab213372 4.6 10,000 0.80 MDC ab208222 4.6 10,000 0.79 Mesothelin ab219027 13.72 30,000 3.49 MIP1 alpha ab208228 1.5 3,333 0.23
MIP1 beta ab208227 1.5 3,333 0.89 MIP2 ab208221 1.5 3,333 0.44 MIP4 ab219028 1.52 3,333 0.20 MIP5 ab219029 4.57 10,000 0.94 MMP13 ab219030 4.57 10,000 1.10 MMP8 ab219031 13.72 30,000 3.20 Myoglobin ab219032 123.46 270,000 41.40 Oncostatin M ab219033 1.52 3,333 0.22 Osteoprotegerin ab219034 4.57 10,000 0.85 PCSK9 ab219035 41.15 90,000 30.60 PF4 ab213373 4.6 10,000 1.45 Procollagen Type 1 ab219036 13.72 30,000 2.30 RANTES ab208212 1.5 3,333 0.77 Serum Albumin ab213376 41.5 90,000 14.38 stnf RI ab219037 4.57 10,000 1.57 TARC ab208219 13.7 30,000 0.79 Tissue Factor ab219038 4.57 10,000 1.20 TNF-alpha ab208205 4.6 10,000 1.37 tpsa ab213383 1.5 3,333 0.25 Transferrin ab219039 370.37 810,000 58.90 Urokinase type Plasminogen Activator ab219040 4.57 10,000 2.30 VE-Cadherin ab219041 1111.11 2,430,000 399.80 VEGF Receptor 2 ab219042 13.72 30,000 4.30
If you can't see the analytes you need, then let us know or check back later - we are constantly developing new assays. Human protein standard mixes All protein standard mixes contain sufficient protein to run a standard curve in duplicate twice. Name Product code Analytes Protein standard mix A ab208204 GM-CSF, IFN-gamma, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-10, IL-12 p70, IL-13, IL-17A, MCP1, TNF alpha Protein standard mix B ab209501 IL-1a, IL-1Ra, IL-6Ra, IL-7, IL-23, IP-10, G-CSF, GRO alpha, MIP2, Eotaxin, MIP1a, MIP1b, RANTES, Fractalkine, TARC, MDC, BCA1 Protein standard mix C ab213371 MCP3, PF4, CXCL16, IL-16, Serum Albumin, C- Reactive Protein, Beta-2-Microglobulin, Leptin, IL-17F, ICAM1, CD40 Ligand, Total Prostate Specific Antigen, CXCL5, Fibrinogen, Carcino Embryonic Antigen, alpha Fetoprotein Protein standard mix D ab218999 Cardiotrophin 1, CD276, CD40, EGF, ICAM2, IL- 15, IL-18, IL-22, IL-3, Interferon alpha 1, Mesothelin, Oncostatin M, Osteoprotegrin Protein standard mix E ab219000 BDNF, BST2, CEACAM1, Coronin 1a, FGFR3, LOX1, MMP13, MMP8, Myoglobin, PCSK9, Tissue Factor, Urokinase type Plasminogen Activator, VE-Cadherin, VEGF Receptor 2 Protein standard mix F ab219001 Apolipoprotein AII, Apolipoprotein A-IV, CD14, CXCL7, Cystatin C, IGFBP3, Liopcalin-2, Lipoprotein A, MIP4, Procollagen Type 1, Transferrin
Mouse pre-designed panels Panel Plex abid Analytes Key cytokines 14 ab213397 TNF alpha, IL6, IL10, IL12 p70, IFN gamma, IL4, IL2, IL5, IL9, IL13, IL17A, GM-CSF, MCP1, IL1 beta Th1/Th2/Th17 7 ab213395 IL2, IL4, IL6, IL10, TNF alpha, IFN gamma, IL17A Th1/Th2 7 ab213394 IL2, IL4, IL5, IL6, IL10, TNF alpha, IFN gamma Inflammation 7 ab213396 IL6, IL10, TNF alpha, IL12 p70, IL1 beta, MCP1, IFN gamma Key cytokines 4 ab213393 TNF alpha, IL6, IFN gamma, IL10 List of mouse analytes Once you ve assembled your custom panel or added extra analytes to a pre-designed panel, we can pre-mix the separate assay reagents into a single vial to save you time. Just request a quote for this option. In rare cases, combinations of analytes cannot be run together in the same panel, but we ll check this when you place an order or request a quote. Assay dynamic ranges can be extended with an alternate extended standard curve, typically 1.5 3,333 becomes 0.61-10,000 and 4.6 10,000 becomes 1.83 30,000. abid Dynamic Range (pg/ml) low high Limit of Detection (pg/ml) Angiogenin ab216804 4.6 10000 1.79 Axl ab215151 41.15 90,000 5.82 beta IG H3 ab216806 123.5 270000 27.58 CCL27 ab215133 13.72 30,000 2.00 CD31 ab215156 13.72 30,000 3.67 CD40 ab215153 4.57 10,000 1.11 Chemerin ab216809 1.5 3333 0.19 Clusterin ab216805 123 270000 44.93
Complement Factor D ab215150 123.46 270,000 25.63 CXCL1 ab209507 1.52 3,333 0.26 CXCL11 ab215138 4.57 10,000 0.72 CXCL16 ab215125 4.57 10,000 0.87 Cystatin C ab216810 1.5 3333 0.72 DKK1 ab215154 13.72 30,000 5.22 E-Cadherin ab216811 123 270000 17.45 EGFR ab216812 123.5 270000 23.34 EMMPRIN ab216813 1.5 3333 0.31 Eotaxin ab215143 1.52 3,333 0.24 Eotaxin-2 ab215130 4.57 10,000 0.92 EphA2 ab215159 4.57 10,000 2.16 E-Selectin ab216807 13.7 30000 2.02 Fractalkine ab215145 13.72 30,000 4.65 Galectin 3 ab216814 13.7 30000 2.83 G-CSF ab215124 1.52 3,333 0.21 GM-CSF ab209514 4.57 10,000 4.11 GRO gamma ab215137 1.52 3,333 0.21 ICAM2 ab216815 13.7 30000 2.54 IFN-gamma ab209522 1.52 3,333 0.24 IL-1 beta ab209513 1.52 3333 0.24 IL-10 ab209519 13.72 30000 3.08 IL-12p70 ab209521 4.57 10000 1.60 IL-13 ab209525 1.52 3333 0.28 IL-16 ab215131 1.52 3333 0.19 IL-17A ab209512 1.52 3333 0.23 IL-17F ab215135 1.52 3333 0.21
IL-1a ab215127 1.52 3333 0.26 IL-2 ab209516 4.57 10000 0.79 IL-27a ab215140 41.15 90000 38.37 IL-33 ab215144 1.52 3333 0.36 IL-4 ab209523 1.52 3333 0.18 IL-5 ab209515 1.52 3333 0.33 IL-6 ab209520 13.72 30000 4.00 IL-6 R alpha ab216802 41.1 90000 7.9 IL-9 ab209524 4.57 10000 3.04 IP-10 ab216803 1.5 3333 0.39 Leptin ab215147 13.72 30000 3.32 Lipocalin-2 ab216816 1.5 3333 0.25 LOX1 ab216817 41.1 90000 5.63 L-Selectin ab216808 1.5 3333 0.36 MCP1 ab209518 4.57 10000 1.41 MCP2 ab215134 1.52 3333 0.18 MCP3 ab215139 1.52 3333 0.21 M-CSF ab215126 13.72 30000 3.84 MDC ab215136 1.52 3333 0.22 Mesothelin ab216818 13.7 30000 3.23 MIF ab216819 41.1 90000 16.88 MIG ab215132 1.52 3333 0.27 MIP1a ab209510 4.57 10000 1.92 MIP1b ab209517 4.57 10000 0.61 MIP2 ab215128 1.52 3333 0.19 NGF R ab216821 41.1 90000 6.43 NOV ab216822 13.7 30000 2.72
Osteoprotegerin ab216823 13.7 30000 3.02 PF4 ab215129 1.52 3333 0.41 PLGF ab216824 1.5 3333 0.22 Prolactin ab215157 123.46 270000 35.70 RAGE ab216825 123 270000 18.61 RANTES ab215141 1.52 3333 0.60 Resistin ab215158 1.52 3333 0.60 Retinol binding protein 4 ab216826 1.5 3333 0.22 Serum Albumin ab215146 41.15 90000 10.35 Stem Cell Factor ab215155 1.52 3333 0.31 stnf RI ab215149 4.57 10000 0.61 stnf RII ab216827 4.6 10000 1.05 TARC ab215152 4.57 10000 1.81 TECK ab215142 4.57 10000 1.93 Thrombomodulin ab216828 41.1 90000 18.91 TNF-alpha ab209511 4.57 10000 2.00 VEGFA ab215148 1.52 3333 0.23 If you can't see the analytes you need, then let us know or check back later - we are constantly developing new assays. Mouse protein standard mixes All protein standard mixes contain sufficient protein to run a standard curve in duplicate twice. Name Product code Analytes Protein standard mix A ab209500 TNF-alpha, IFN-gamma, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, MCP1, GM-CSF, CXCL1, MIP1a, MIP1b Protein standard mix B ab215122 G-CSF, CXCL16, M-CSF, IL-1a, Eotaxin-2, IL- 16, MIG, CCL27, MCP2, IL-17F, MDC, GRO
gamma, MCP3, RANTES, Eotaxin, IL-33, Complement Factor D, Resistin Protein standard mix C ab215123 MIP2, PF4, CXCL11, IL-27a, TECK, Fractalkine, Serum Albumin, Leptin, VEGFA, stnf RI, Axl, TARC, CD40, DKK1, Stem Cell Factor, CD31,Prolactin, EphA2 Protein standard mix D ab216800 IP-10, beta IG H3, EGFR, EMMPRIN, Galectin 3, ICAM2, LOX1, Mesothelin, MIF, NGF R, Osteoprotegrin, stnf RII Protein standard mix E ab216801 Angiogenin, Clusterin, E-Selectin, L- Selectin, Chemerin, Cystatin C, E- Cadherin, Lipocalin-2, NOV, PLGF, RAGE, RBP4, Thrombomodulin, MMP3 IL-6 R alpha protein standard ab217149 IL-6 R alpha