Heidi Rossow, PhD UC Davis School Of Veterinary Medicine, VMTRC Tulare, CA. Interpreting Forage Quality from the Cows Perspective

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Heidi Rossow, PhD UC Davis School Of Veterinary Medicine, VMTRC Tulare, CA Interpreting Forage Quality from the Cows Perspective

Interpretation of chemical nutrient analyses Plant Nutrient Analyses Digestibility (Animal) Physical attributes of forage

Nutrients Water (DM and DMI) Energy (NE, DE, ME) Protein (CP, RUP, RDP, MP, SP, NPN) Fat (Triglycerides, plant lipids) Carbohydrates (NDF, ADF, Starch, SC, Cellulose, Pectins, Hemicellulose, Lignin, Organic acids, NSC, NFC, ESC, WSC, Fructans) Minerals (Ash) Vitamins

Nutrients - DM Dry Matter (%DM) = Amount of feed w/out H20 100*Weight of dry feed / Weight As Fed Analysis : Oven or microwave drying Alfalfa Hay = 85-90% Corn Silage = 30-45% Corn grain = 88-91%

Ruminants

Nutrients - Energy Alfalfa Hay = 1.27 mcal/kg Corn Silage = 1.45 mcal/kg Corn Grain = 2.0 mcal/kg Ingested Energy Digestible E 70% Feces E 30% Urine 5% & Gas 5% Metabolizable E 60% Net E 40% (Recovered E) Heat 20% (tissue+lactation+conception E)

Nutrients - Protein Total N Crude Protein RUP RDP (includes NPN) Unavailable Digestion Microbes CP RUP RDP Alfalfa Hay 16-23% 17-25% 75-83% Corn Silage 8-10% 30-40% 60-70% Corn grain 9-10% 30-50% 50-80% Digestion

Nutrients - Fat Ether Extract: Fats, oils, waxes, vitamins ADEK FA Analysis: Gas Chromotography EE Alfalfa Hay 2-4% Corn Silage 2.5-3% Corn Grain 4%

Nutrients - Minerals Ash Analysis: Total mineral content muffle oven, ICPMS digestion Macro : Ca, P, Na, Cl, K, Mg, S Micro : Co, Cu, I, Fe, Mn, Mb, Se, Zn, Cr Analysis: Boiled in acid, AAS, ICPMS Ash Ca P K Alfalfa Hay% 10 1.5 0.3 1.7 Corn Silage% 4-5 0.28 0.25 1.0 Corn Grain% 1.5 0.04 0.3 0.4

Interpretation of chemical nutrient analyses Plant Nutrient Analyses Digestibility (Animal) Physical attributes of forage

Carbohydrates (Plant)

Nutrients - Carbohydrates

Nutrients - Carbohydrates NDF = Neutral detergent fiber Cell Wall (NDF Residue) = cellulose, hemicellulose, lignin Cell Contents (NDF Soluble) = protein, NPN, lipid, starch, sugars, fructans, organic acids NDF NDICP (%DM) Alfalfa Hay 35-50% 2-3% Corn Silage 45-70% 1.3% Corn Grain 9-10% 0.7%

Nutrients - Carbohydrates ADF = Acid detergent fiber ADF Residue = Cellulose, Lignin ADF Soluble = everything else ADFIN Acid Detergent Insoluble N = Bound N (unavailable to cow) ADF ADICP Lignin Alfalfa Hay 25-40% 1.5% 6-7% Corn Silage 28-35% 0.9% 3.4% Corn Grain 3.5% 0.3% 0.9%

Nutrients - Carbohydrates

Nutrients - Carbohydrates NSC = Non structural carbohydrate (enzymatic) (starch, fructans, sugars) NFC = Non fiber carbohydrate (by difference) (starch, sugars, pectins) NFC = 100-(NDF+CP+Fat+Ash) Starch = amylose, amylopectin NSC NFC Starch Sugar Pectin Alfalfa Hay % 12.5 22.0 24.5 20 33 Corn Silage % 34.7 41.0 71.3 0 0 Corn Grain % 68.7 67.5 80.0 20.9 0

Nutrients (Corn Silage) Yeast < 100,000 cfu ph < 5 Mold < 600,000 cfu Mold Types Total VFA = 10-14% Lactic = 3-7%, > 60% of total Acetic = 1-3.5% Lactic / acetic > 3:1 Propionic 0-1% Butyric < 0.1% Ethanol = 1-3% Ammonia = 5-7% Temp = Depends on stage, Ambient

Interpretation of chemical nutrient analyses Plant Nutrient Analyses Digestibility (Animal) Physical attributes of forage

Digestibility

Animal Response Ingested Digestible Feces Urine & Gas Metabolizable Bioavailable Net (tissue+lactation+conception)

TDN Related to Proximate Analysis, represents energy content of feeds TDN = digestible NFE + digestible CF + digestible protein + digestible EE*2.25 OR TDN = DE/0.044 TDN Alfalfa Hay 55-60% Corn Silage 65% Corn Grain 85-92%

Alfalfa Digestibility Stage %Digestibility Pre-bud 66.8 Bud 65 Early Bloom 63.1 Mid-Bloom 61.3 Full-Bloom 59.4 Late-Bloom 57.5 Mature 55.8 GROWTH Stage %Leaves % CP %ADF %NDF RFV Bud >40 >18 <30 <40 >140 Early Bloom 30-40 16-18 30-35 40-45 124-140 Mid-Bloom 20-30 13-16 35-40 45-50 100 Full-Bloom <20 <12 >40 >50 <100

Interpretation of chemical nutrient analyses Plant Nutrient Analyses Digestibility (Animal) Physical attributes of forage

Physical Attributes Forage length Sorting (Silage packing) (Shear Force) Particle Size Rumination, Rumen ph Processing / Processing score Digestion Fecal Starch, Fecal Sorting, Fecal Scoring - Digestion

Forage Length

Particle Size

Particle Size

Corn Processing Processing: Whole Cracked Steam Flaked Dry Rolled Ground Shifts site of digestion and Starch digestibility Rumen (DR 34% SF Thin 45% SF Thick 27%) Intestines (DR 44% SF Thin 50% SF Thick 58%)

Corn Silage Processing Score Coarse Fraction > 4.75mm (Starch) Fine Fraction < 1.18mm (NDF) % Starch passing coarse screen Ranking Greater than 70% Optimum 50% to 70% Average Less than 50% Inadequate

Fecal Starch Enzymatic lab analyses ($17- $20) Fecal Starch < 4.5% 1% = 0.67 lbs Milk Corn in Feces, fecal starch >10%

Fecal Sorting and Scoring

Fecal Sorting

Thanks