2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Paediatric Histopathology Western Bank Sheffield S10 2TH United Kingdom Contact: Tel: +44 (0) 1142 717240 Fax: +44 (0) 1142 706121 E-Mail: Karen.Bennett@sch.nhs.uk Website: www.sheffieldchildrens.nhs.uk/ourservices/laboratory-medicine/ Additional site activity performed away from the laboratory Testing performed at the above address only Location details Activity On-site Mortuary and Body Store Storage of bodies Retrieval of tissue via post mortem Record keeping (body identification and audit trail) Assessment Manager: RBB Page 1 of 14
DETAIL OF ACCREDITATION HUMAN BODY TISSUE resection specimens diagnosis purpose of diagnosis Specimen Dissection SOP 326.4.100 - General specimen reception procedures. SOP 327.1.1 - Photography QPulse Asset module - HIS023 - HIS161 SOP 326.4.311 - Surgical samples - cut up SOP 326.4.346 - Handling fresh samples SOP 326.4.310 - Tumours & Lymph nodes (lymphomas) SOP 322.3 - Macro micro form SOP 322.5 - Gastrointestinal biopsy macro sheet SOP 326.4.311 - Surgical samples - cut up Processing Thermo Shandon Excelsior Tissue Processor & Leica ASP300S SOP 326.4.422 - Tissue Processing schedules SOP 326.4.424 - Tissue processor - General use Microtomy Leica Microtomes for sectioning blocks to produce 4um thick ribbons Assessment Manager: RBB Page 2 of 14
Unfixed Body fluids Unfixed Cell washings Unfixed Fine Needle Aspirates Cell slide preparations Cell block preparations purpose of Special stains to identify the following: Acid Mucins Mucins Amyloid Mast cells and white cells (granulocytes), of myeloid lineage Microtomy RA Lamb and Thermo Shandon Water baths set at 45⁰C for floating sections SOP 326.4.334 Microtomy Non Gynae Cytology SOP 326.4.302 - Types of cytology specimens SOP 326.4.300 - Receipt of cytology and Surgical specimens SOP 326.4.304 - Cytospin preparation Haematoxylin and Eosin stain Automated and manual Leica AutoStainer XL automated H&E stainer Leica CV 5030 Coverslipper automated coverslipper SOP 326.3.032 - Haematoxylin & Eosin SOP 326.4.428 - Leica Autostainer XL & Coverslipper CV5030 Special staining Manual SOP 326.3.002 Alcian Blue/PAS SOP 326.3.003 Alcian Blue SOP 326.3.003A Alcian blue ph 1.0 SOP 326.3.010 Congo Red SOP 326.3.013 Chloroacetate Esterase - Leder's Assessment Manager: RBB Page 3 of 14
purpose of Special stains to identify the following: (cont d) Remove deposits of mineral Micro-organisms and Protozoa Elastic fibres Reticulin fibres Special staining Manual SOP 326.3.016 EDTA SOP 326.3.020 Giemsa SOP 326.3.024 Elastic Stain (Modified Miller's) SOP 326.3.026 Gordon and Sweet's Reticulin Gram +ve micro-organisms Gram +ve and -ve micro-organisms Argyrophil cells Fungi and Pneumocystis carinii Myelin Fibrin SOP 326.3.027 Gram +ve SOP 326.3.028 Gram Twort Method SOP 326.3.029 Grimelius Silver Method SOP 326.3.030 Grocott's Silver Technique SOP 326.3.031 Grocott Methanamine Silver rapid Method SOP 326.3.038 Luxol Fast Blue standard method SOP 326.3.040 Luxol Fast Blue/Cresyl Fast Violet SOP 326.3.041 MSB (Martius Yellow, Crystal Scarlet, Soluble Blue) Assessment Manager: RBB Page 4 of 14
purpose of Special stains to identify the following: (cont d) Melanin Connective tissues Hematopoietic cells Basement membranes Helicobacter Copper associated protein, elastic and Hepatitis B surface antigen (HBSAg) Glycogen, Basement membranes Fungi Glycogen Viral inclusions and keratin Astrocytes, Neuroglial cells and Fibres Removal of pigments Lipofucsin Special staining Manual SOP 326.3.042 Masson's Fontana SOP 326.3.043b Masson's Trichrome Blue method SOP 326.3.045 May Grunwald Giemsa SOP 326.3.046 MST (Methanamine Silver Technique) Jones SOP 326.3.047 Giemsa (Modified) Helicobacter SOP 326.3.051 Shikata's (Modified Orcein) SOP 326.3.056 PAS (Periodic Acid Schiff) SOP 326.3.057 PAS - Diastase SOP 326.3.059 Phloxine Tartrazine SOP 326.3.061 PTAH for Glia SOP 326.3.062 Pigments (Removal of) SOP 326.3.070 Sudan Black Assessment Manager: RBB Page 5 of 14
purpose of Special stains to identify the following: (cont d) Special staining Manual Urine Mast cells SOP 326.3.071 Toluidine Blue Metachromatic granules Calcium Mycobacterium leprae Spirochaetes and other non filamentous bacteria Acid and alcohol fast bacilli Nuclear stain Iron Melanin Connective tissues SOP 326.3.073 Toluidine Blue for Metachromatic Dystrophy SOP 326.3.076 Von Kossa SOP 326.3.077 Wade Fite SOP 326.3.078 Warthin Starry SOP 326.3.079 Ziehl-Neelsen (ZN) SOP 326.3.080 Weigert's Iron Haematoxylin SOP 326.3.081 Perl's Prussian Blue SOP 326.3.084 Masson Hamperl (Singh's Modification) SOP 326.3.085 Picro Sirius Red Special staining (Non-Gynae Cytology) Manual Metachromatic leuko-dystrophy Glycogen SOP 326.3.073 - Toluidine blue for metachromatic leuko-dystrophy SOP 326.3.056 PAS (Periodic Acid Schiff) Assessment Manager: RBB Page 6 of 14
purpose of Special stains to identify the following: (cont d) Routine staining for identification of basophilic and eosinophilic structures Fungi and Pneumocystis carinii Special staining (Non-Gynae Cytology) Manual SOP 326.3.032 - Haematoxylin & Eosin SOP 326.3.030 Grocott's Silver Technique Immunohistochemistry Automated Dako Autostainer Link 48 automated stainer for use with a wide range of antibodies SOP 326.1.010 - Dako Autostainer Link 48 SOPs that cover Immunocytochemistry are 326.1.001 to 326.1.013. The main ones are: SOP 326.1.001 - Immunocytochemistry general procedures SOP 326.1.004 - Immunocytochemsitry reagents SOP 326.1.013 - Control material for Immunocytochemistry SOP 341.1.004 - UKNEQAS ICC SOP 327.1.008 - Criteria for the rejection of immunocytochemistry Assessment Manager: RBB Page 7 of 14
purpose of Immunocytochemistry for the identification of: Smooth Muscle/ myofibroblast Adenovirus Alpha1 antitrypsin genetic diseases/ various tumours Cytogenetic marker Some lymphomas Heptocellular carcinoma/ germ cell tumours Diagnostic utility lymphomas/ bone marrow biopsies Axonal damage Various carcinomas Smooth muscle Mesothelial marker, some tumours and Hirschsprungs Early hypoxic cellular damage Diagnostic utility lymphomas/ bone marrow biopsies Immunohistochemistry Automated And antibodies as appropriate:- Actin (Smooth muscle) Adenovirus Alpha1 Anti trypsin AE1&3 (Cytokeratin) ALK1 Protein Alpha Feto Protein BCL-2 BCL-6 Beta-APP Beta Catenin Caldesmon Calponin Calretinin C9 CD1a (010) Assessment Manager: RBB Page 8 of 14
purpose of Immunocytochemistry for the identification of: (cont d) Diagnostic utility lymphomas/ bone marrow biopsies (cont d) Immunohistochemistry Automated And antibodies as appropriate:- CD3 CD4 CD7 CD8 CD10 (Calla) CD15 CD20 (L26) CD21 (1F8) CD23 CD30 (Kil) Ber H2 CD44 CD45 (LCA) CD45RO (UCLH1) CD56 (NCAM) CD61 CD68 (PGM1) CD79a Assessment Manager: RBB Page 9 of 14
purpose of Immunocytochemistry for the identification of: (cont d) Diagnostic utility lymphomas/ bone marrow biopsies (cont d) Endothelial marker Stromal cells GIST, Haematopoietic progentitor, Mast cells Immunohistochemistry Automated And antibodies as appropriate:- CD99 CD31 CD34 (QB-End10) CD117 (C-KIT) Bone Biopsies/ Erythroid cells CD235a (Glycophorin A) Intestinal metaplasia/ Cancer colorectal origin Neuroendocrine differentiation tumours/ medullary carcinoma thyroid Identification primary site carcinomas Cytomegalovirus CDX2 Chromogranin A CK7 CMV Lymphatic endothelium D2-40 Smooth muscle marker Muscle biopsy Desmin D33 DYS1 DYS2 DYS3 Assessment Manager: RBB Page 10 of 14
purpose of Immunocytochemistry for the identification of: (cont d) Most normal and neoplastic epithelium/ some lymphomas Endothelial markers Dermal dendrocytes (skin/ soft tissue markers) Astrocytes, schwann cells and pheripheral nerve tumours Infantile haemangioma Trophoblast/ germ cell tumours Be cells, B lineage ALL, most nonhodgkins lymphomas Immunohistochemistry Automated And antibodies as appropriate:- EMA Factor VIII Factor XIIIa GFAP GLUT-1 HCG HLA-DR H. Pylori H. Pylori Herpes Epitheliod sarcoma, atypical teratois/ rhabdoid tumour Tumour cell proliferation Mast cells Muscle biopsies Neutrophils, Granulocytic sarcoma HSV INI-1 Ki67 Mast Cell Tryptase (MCT) Merosin (M-Chain) Myeloperoxidase Assessment Manager: RBB Page 11 of 14
purpose of Immunocytochemistry for the identification of: (cont d) Differentation hepatocellular carcinoma from cholangiocarcinoma Skeletal muscle differentiation Muscle biopsy Skeletal muscle differentiation Be cell lineage/ Lymphoma Neuroblastoma/ Small round cell tumour Neurofilament protein Neurosecretery cells Parvovirus Immunohistochemistry Automated And antibodies as appropriate:- MOC 31 Myo-D Myosin Slow Myogenin MUM-1 NB84 NFP NSE Parvovirus Neuroendocrine differentiation PGP 9.5 Trophoblastic tumours Muscle biopsy PLAP Sarcoglycan Alpha Sarcoglycan Beta Sarcoglycan Gamma Spectrin Assessment Manager: RBB Page 12 of 14
Fresh Human Tissue purpose of Immunocytochemistry for the identification of: (cont d) Muscle biopsy (cont d) Differential skin, soft tissue & neural lesions Neuroendocrine marker Differential leukaemia/ lymphomas Differential for a variety of benign lesions and tumours Wilm's tumour Examination of fresh frozen tissue to rapidly identify or exclude morphological and cytological abnormalities for the purpose of diagnosis Routine staining for identification of basophilic and eosinophilic structures Connective tissue Glycogen, Basement membranes Fungi Immunohistochemistry Automated And antibodies as appropriate:- Myosin Slow Myosin Fast S100 Synaptophysin TdT Vimentin Wilm's Tumour (WT1) Leica CM1900 & Leica CM1850 used for sectioning frozen fresh tissue SOP 326.3.032 - Haematoxylin & Eosin SOP 326.2.013 - Van Gieson Frozen Section SOP 326.2.016 - Cell PAS Frozen Section SOP 326.2.010 Cryotomy Assessment Manager: RBB Page 13 of 14
purpose of diagnosis Interpretive Microscopy Nikon Microscope Olympus Microscope SOP 341.1.004 - UKNEQAS Immunocytochemistry SOP 341.1.003 - UKNEQAS Cellular Pathology - General Pathology Module. SOP 341.1.003a - UKNEQAS Cellular pathology - Muscle Histochemistry. SOP 326.9.006 - Internal assessment of quality of Histological Preparations. SOP 327.2.004 - Writing Surgical reports. SOP 321.1.054 - Revised Reports. SOP 321.1.055 - Reporting frozen sections. SOP 326.2.004a - Muscular biopsy report. SOP 321.1.042 - Oncology reporting. SOP 321.1.047 - Neonatal/ Child post mortem report. SOP 321.1.052 - Coroners post mortem report. SOP 321.1.046 - Fetal/ Perinatal post mortem report. SOP 326.4.341 - Macroscopic description of singleton placenta. SOP 326.4.342 - Macroscopic description of placenta - twin SOP 326.4.341a - Placental histology comments END Assessment Manager: RBB Page 14 of 14