2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Unit 5 Contact: Mr Paul Hancock Berkeley Business Park Tel: +44 (0)1905 751300 Wainwright Road Fax: +44 (0)1905 751301 Worcester E-Mail: scientificservices@worcestershire.gov.uk WR4 9FA Website: www.worcestershire.gov.uk/scientificservices Testing performed at the above address only DETAIL OF ACCREDITATION ANIMAL FEEDING STUFFS Chemical Tests Documented In-House Methods Crude oil and fat Moisture Nitrogen and crude protein Ash Crude Fibre A02 using acid digestion, solvent extraction and gravimetry A01B using gravimetric technique FGEN39 using Rapid N Dumas system A08 using gravimetric technique A06 using Fibertec M6 system FOOD Chemical Tests Documented In-House Methods Baking powder Available and Residual CO2 F0102 Cocoa and chocolate products, Cocoa, Chocolate Theobromine Total Fat F0601 using HPLC F0604 using Soxhlet method Fish The identification of fish species F2402 using PCR-RFLP analysis using the Agilent 2100 Bioanalyser Frozen, cooked and peeled prawns Determination of ice glaze content F2433 Flour, self-raising Available and Residual CO2 F0410 Fruit products Dry soluble residue (soluble solids) F3201 using refractometry Fruit juices and fortified drinks Vitamin C FGEN50 by HPLC with UV detection Honey Moisture F6101 using refractometry Assessment Manager: ED Page 1 of 6
FOOD (cont d) Chemical Tests (cont d) Documented In-House Methods Honey (cont d) Hydroxymethylfurfural F6104A using HPLC with UV detection Diastase activity F6105 using spectrophotometry and based on European Honey Commission Regulations 2003 Ice cream Fat F1128 using alkaline hydrolysis Alcoholic beverages Alcoholic beverages (cont d) Alcohol: 25% - 100% Congeners covering: acetaldehyde iso-amyl alcohol methanol 2-methyl-propanol propan-1-ol F1706 using density meter F1704 using GC Wine Sulphur dioxide FGEN01 using distillation and titrimetry Alcoholic beverages & foods Alcohol: 0.02% - 25% F1701 using GC Meat - fresh or frozen Beta-HADH Activity F4404 by an Enzymatic technique Meat and meat products Meat content (by calculation) F4546/F4552 Standard operating procedure for the calculation of meat content including: F4560B, Fat free meat F4547 Carbohydrate by difference F4541B Collagen in meat protein ratio F4562A Excess Fat, not included in meat calculation F4564A excess connective tissue Hydroxyproline Nitrogen Soya F4501 based on BS4401:Part 11:1995 FGEN39 using Rapid N Dumas System F4519 using immunoassay techniques Assessment Manager: ED Page 2 of 6
FOOD (cont d) Chemical Tests (cont d) Documented In-House Methods Milk, liquid Fat F1128 using alkaline hydrolysis Freezing Point Depression Titratable acidity Total solids F1105 using cryoscope F1108 using titration F1101 using gravimetric technique. Milk products Fat F1128 using alkaline hydrolysis Oils and fats Acidity and acid value F6002 using titration Foods (General) Beverages Sugar and sugar confectionery Peroxide index Butter fat in fat Fatty acid composition: Saturates Mono-unsaturates Poly-unsaturates Trans fatty acids Sweeteners and preservatives: Aspartame Acesulfame K Caffeine Saccharin Sorbic acid Benzoic acid Sugars: Fructose Glucose Lactose Maltose Sucrose F6001 using titration F6003 using Gas-Liquid Chromatography F6006 using Gas-Liquid Chromatography based on BS EN ISO 12966-2:2011 F2102 using HPLC F6399 using HPLC Assessment Manager: ED Page 3 of 6
FOOD (cont'd) Chemical Tests (cont'd) Documented In-House Methods Soft drinks and confectionery FOOD Cereals, dried fruit, spices and herbs and ANIMAL FEEDING STUFFS Water soluble artificial colourings (i.d. and quantification) Allura red Amararanth Carmoisine Erythrosine Ponceau 4R Red 2 G Quinoline Yellow Sunset Yellow Brilliant Blue Patent Blue V Green S Indigo Carmine Tartrazine Development and modification of methods/analysis Aflatoxins G1, G2, B1, B2 FGEN17 by HPLC with DAD detector Methods modified/developed under the protocols identified in the Generic Methods File using the techniques of HPLC and GC, Generic Protocol G1 to G3 F5102 using Rhone Diagnostics Easi Extract columns and HPLC with detection by Kobra FOOD Ash F4517 Incineration at 525 ± 25 C and gravimetric technique Dietary fibre Energy content (by calculation) Fat Moisture Nitrogen and crude protein FDFIBRE using colorimetry by Fiberzym kit Standard operating procedure for the calculation of energy content F4544/F4543 FGEN27 using acid digestion and soxhlet extraction F4521 Drying at 102 ± 2 C and gravimetric technique FGEN39 using Rapid N Dumas System Assessment Manager: ED Page 4 of 6
FOOD (cont'd) Chemical Tests (cont'd) Documented In-House Methods Ochratoxin A ph Salt (expressed as Sodium Chloride) Sodium Sorbic and Benzoic acid Sulphur dioxide Total volatile bases/total volatile nitrogen FGEN30 by immunoaffinity column clean-up and HPLC fluorescence FpH by ph electrode based on AOAC 981.2, 960.19 and 945.10 FNaICP1i10 by stoichiometric calculation based on Sodium results FNaICP1i10 using acid digestion followed by Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) FGEN20 using HPLC FGEN01 using distillation and titrimetry FGEN02 using titrimetry Physical Tests Microscopical examination of herbs, spices and other vegetable materials FGEN24 Optical Microscopy FOODS, General and Animal Feeding stuffs Rice and Rice Products Meat and Meat Products Molecular Tests Detection and quantification of Round-up Ready Soya Detection of genetic events p35s, tnos and CryAb/Ac Species identification of beef, lamb, pork, goat, chicken, and horse (0.1% LOD) FGMO05 using Real Time PCR FGMO12/13 using SYBR-Green Real Time PCR for the detection of the genetic events p35s, tnos and CryAb/Ac F4407 by detection of mitochondrial DNA using real time qpcr Assessment Manager: ED Page 5 of 6
Materials and articles in contact with food Chemical Tests Plastic materials in contact with food overall migration into: (1) Selection of conditions and test methods for overall migration (2) Overall migration into vegetable oil by total immersion (3) Overall migration into aqueous simulants by total immersion (4) Overall migration into aqueous simulants by article filling (5) Substitute tests for overall migration from plastics intended to come into contact with fatty foodstuffs using test media isooctane and 95% ethanol Documented In-House Methods based on: FGEN25I based on BS EN 1186-1:2002, inm accordance withcommission Regulation (EU) No 10/2011 on plastic materials and articles FGEN25A based BS EN 1186-2:2002, in accordance with Commission Regulation (EU) No Regulation 10/2011 on plastic materials and articles FGEN25B based on BS EN 1186-3:2002, in accordance withcommission Regulation (EU) 10/2011 on plastic materials and articles FGEN25D based on BS EN 1186-9:2002, in accordance with Commission Regulation (EU) No 10/2011 on plastic materials and articles FGEN25G based on BS EN 1186-14:2002 END Assessment Manager: ED Page 6 of 6