OIE Reference Laboratory Reports Activities Activities in 2016 This report has been submitted : 2016-12-30 08:33:00 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Foot and mouth disease Address of laboratory: 177, Hyeoksin 8-ro Gimcheon-si Gyeongsangbukdo, 39660 KOREA (REP. OF) Tel.: +82-54 912.09.06 Fax: +82-54 912.08.90 E-mail address: parkjhvet@korea.kr Website: www.qia.go.kr Name (including Title) of Head of Laboratory (Responsible Official): Dr. Sung-Hwan Wee Director of FMD Division Name (including Title and Position) of OIE Reference Expert: Dr. Jong-Hyeon Park FMD OIE expert Senior Veterinary Researcher Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities, 2016 1
ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally FMD Ab tests(sp&nsp) 14,140 Direct diagnostic tests Nationally Internationally Virus Isolation 18 Antigen ELISA 1 (realtime) RT-PCR) 19,950 VP1 gene Sequencing 21 ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2016
Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients FMDV antibody detection kits NSP ELISA (1) Commercia 440T LAOS Africa America s Asia and Pacific Europe Middle East FMDV antibody detection kits NSP ELISA (2) Commercia 440T LAOS Africa America s Asia and Pacific Europe Middle East FMDV antigen kits (FMDV Rapid diagnostickits) Rapid Ag kit Commercia 105T LAOS Africa America s Asia and Pacific Europe Middle East 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? OIE Reference Laboratory Reports Activities, 2016 3
ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided LAOS Development and selection of FMD vaccine Improve knowledge on vaccine development process and vaccine strain selection with animal tests ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country Comparative studies for avian influenza virus and foot-andmouth disease virus between Korea and Vietnam 9 years(2016-2024) To carry out Comparative studies of Avian influenza virus and Foot and mouth disease virus between Korea and Vietnam National Center for Veterinary Diagnosis, Department of Animal Health, Hanoi VIETNAM Annual scientific seminar on contagious animal disease including FMD Annually Preparedness for FMD outbreaks Animal Health Laboratory NEW ZEALAND To differentiate the FMDV serotype as the third OIE reference laboratory On going To differentiate the FMDV serotype as the third OIE reference laboratory Regional Reference laboratory for FMD in SEA, Pakchong THAILAND 4 OIE Reference Laboratory Reports Activities, 2016
ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 5 1. Seo-Yong Lee, Mi-Kyeong Ko, Kwang-Nyeong Lee, Joo-Hyung Choi, Su-Hwa You, Hyun-Mi Pyo, Myoung-Heon Lee, Byounghan Kim, Jong-Soo Lee, Jong-Hyeon Park. Application of mouse model for effective evaluation of footand-mouth disease vaccine, Vaccine 34 (2016) 3731 3737. 2. Jong-Hyeon Park, Dongseob Tark, Kwang-Nyeong Lee, Seo-Yong Lee, Mi-Kyeong Ko, Hyang-Sim Lee, Su-Mi Kim, Young-Joon Ko, Min-Goo Seo, Ji-Eun Chun, Myoung-Heon Lee, Byounghan Kim,vel foot-and-mouth disease virus in Korea, July-August 2014, Clin Exp Vaccine Res 5 (2016) 83-87. 3. Jeong-Nam Park, Mi-Kyeong Ko, Rae-Hyung Kim, Min-Eun Park, Seo-Yong Lee, Ji-Eun Yoon, Joo-Hyung Choi, Su- Hwa You, Jung-Won Park, Kwang-Nyeong Lee, Ji-Eun Chun, Su-Mi Kim, Dongseob Tark, Hyang-Sim Lee, Young-Joon Ko, Byounghan Kim, Myoung-Heon Lee, Jong-Hyeon Park. Construction of stabilized and tagged foot-and-mouth disease virus. Journal of Virological Methods 237 (2016) 187nd-m. 4. Su-Hwa You, Hyun Mi Pyo, Seo-Yong Lee, Mi-Kyeong Ko, Joo-Hyung Choi, Sung Ho Shin, Kwang-Nyeong Lee, Byounghan Kim, Jong-Hyeon Park Complete genome sequence of foot-and-mouth disease virus serotype SAT3 Zimbabwe/4/81, Genome announcement 4(4) e00801-16. 5. Min-Eun Park, Seo-Yong Lee, Rae-Hyung Kim, Mi-Kyeong Ko, Jeong-Nam Park, Kwang-Nyeong Lee, Su-Mi Kim, Joo-Hyung Choi, Su-Hwa You, Byounghan Kim, Jong-Soo Lee, Jong-Hyeon Park. Altered adjuvant of foot-and-mouth disease vaccine improves immune response and protection from virus challenge, Trials in Vaccinology 5 (2016) 97 104. b) International conferences: 4 1.J.H. Park, FMD situation in Korea and activity of FMD reference lab, QIA in 2016,. (The 11th OIE/FAO FMD Reference Laboratories Network Annual Meeting) 2. B.K. Ku, J.J Nah, S.Y. Ryoo, T.S. Kim, M.G. Sagong, S.M. Lee, K.N Lee, D.S. Tark, Y.J. Ko,H.M. Pyo, M.K Shin, J.H. Park, M.H. Lee, S.H. Wee, Genetic characterization of the 2016 foot-and-mouth disease viruses in South Korea.(2016 EuFMD, Open session) 3. S.Y. Lee, M.K. Ko, K.N. Lee, J.H. Choi, S.H. You, H.M. Pyo, M.H. Lee, B. Kim, J.S. Lee, J.H. Park, Application of mouse model for effective evaluation of FMD vaccine. (2016 EuFMD, Open session) 4. Mi-Young Park, Young-Joon Ko, Hyun Mi Pyo, Sun Young Park, ChoongSu Kim, Dong Jin Chae, Sung Bin Im, Sung Hwan Wee1 and Dongseob Tark O, Nationwide Serological surveillance of FMDV antibodies in South Korea during 2014~2016 (Conference Research of World Animal Disease, 2016) c) National conferences: 8 1. Taeseong Kim, Soyoon Ryoo, Jinju Nah, Mingun Sagong, Sumee Lee, Ki Sik Choung, Sung-Hwan Wee, Bok Kyung Ku, Sensitive detection method of Foot-and-mouth disease virus in pigs by oral-fluid using cotton rope. O (National conference for the Korean Society of Veterinary Science, Oct 2016) 2. Mingeun Sagong, Jin-Ju Nah, Soyoon Ryoo, Sung-Hwan Wee, Taesung Kim, Sumi Lee, Ki Sik Choung, Bok Kyung Ku, Development of a new prototype of Point Of Care testing (POC) for Differentiation of Foot-and-Mouth sensitive Disease Virus Serotype O (National conference for the Korean Society of Veterinary Science, Oct 2016) 3. Mi-Young Park, Young-Joon Ko, Hyun Mi Pyo, Sun Young Park, ChoongSu Kim, Dong Jin Chae, Sung Bin Im, Sung OIE Reference Laboratory Reports Activities, 2016 5
Hwan Wee1 and Dongseob Tark O, Nationwide Serological surveillance of FMDV antibodies in South Korea during 2014~2016 (National conference for the Korean Society of Veterinary Science, Oct 2016) 4. Chungsu Kim, Sun Young Park, Mi-Young Park, Hyun Mi Pyo, Sunghwan Wee, Comparative evaluation of three serological tests for the detection of vaccine induced antibody of foot-and-mouth disease virus (National conference for the Korean Society of Veterinary Science, Oct 2016) 5. Dong Jin Chae, Sung Bin Im, Mi-Young Park, Hyun Mi Pyo, Sun Young Park, ChoongSu Kim, Dongseob Tark, Sung Hwan Wee, Serological surveillance of FMDV antibodies in South Korea during January to August 20 16 O (National conference for the Korean Society of Veterinary Science, Oct 2016) 6. Min Yeong Kim, Ji-Hyeon Hwang, Kwang-Nyeong Lee, Seo-Yong Lee, Jang-Kwan Hong, Sung-Ho Shin, Jong- Hyeon Park, Byounghan Kim, Field study on Foot-and-Mouth-Disease Vaccination Using Needless Transdermal device in Pigs. (National conference for The Korean Society of Veterinary Science, Oct. 2016) 7. Ji-Hyeon Hwang, Min Yeong Kim, Kwang-Nyeong Lee, Jong-Hyeon Park, Byounghan Kim, Cultivation of Foot-and-mouth disease virus vaccine candidates of Serotype O in Bioreactor. (National conference for The Korean Society of Veterinary Science,Oct. 2016) 8. Efficacy of two commercial monovalent vaccines (O Campos, O Primorsky) against a heterologous strain, FMDV O Jinchun strain, in pigs, Hyejun Jo, Tae Young Lee, Changhee Han, Jaejo Kim, Byounghan Kim, Yong Joo Kim,, National conference for The Korean Society of Veterinary Science,Oct. 2016 d) Other: (Provide website address or link to appropriate information) 0 ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 1 b) Seminars: 1 c) Hands-on training courses: 4 d) Internships (>1 month): 0 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country a Thailand 2 b Thailand 2 c Fiji 2 c Bangladesh 1 c Sri Lanka 3 c Laos 3 6 OIE Reference Laboratory Reports Activities, 2016
ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO/IEC 17025 Certificate scan (PDF, JPG, PNG format) KT372_KOLAS ISOIEC 17025.pdf 16. Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited Antigen detection(realtimert-pcr, RT-PCR, AntigenELISA) Antibody detection(sp ELISA, NSP ELISA) Accreditation body KOLAS-Korean Laboratory Accrediation Scheme KOLAS-Korean Laboratory Accrediation Scheme 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 1.1.4) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? OIE Reference Laboratory Reports Activities, 2016 7
Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented 2016 EUFMD 26th~28th Oct 2016 Portuga 2 presenting posters Ref 14. b) International conferences The 11th OIE/FAO FMD Reference Laboratories Network Annual Meeting 30th v~ 2nd Dec 2016 France Speaker Ref 14. b) International conferences ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? Purpose of the proficiency tests: 1 Role of your Reference Laboratory (organiser/ participant). participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. FMD and SVD Proficiency tests in 2016 (UK) Participant 1 Various/WRLFMD(UK) 1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? ToR 11: To organise inter-laboratory proficiency testing with laboratories other 8 OIE Reference Laboratory Reports Activities, 2016
than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: http://www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3 Purpose for inter-laboratory test comparisons 1. participating laboratories Region(s) of participating OIE Member Countries National Proficiency test for diagnosis of FMD(Organiser) 45 provincial animal health Africa Americas Asia and Pacific Europe Middle East ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? 25. Additional comments regarding your report: OIE Reference Laboratory Reports Activities, 2016 9