PERENNIAL RYEGRASS SEED PRODUCTION RESEARCH ROSEAU/LAKE OF THE WOODS,MINNESOTA- 2010

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PERENNIAL RYEGRASS SEED PRODUCTION RESEARCH ROSEAU/LAKE OF THE WOODS,MINNESOTA- 2010 Perennial Ryegrass Seed Production Meeting -Roseau December 15, 2010 Presented by Donn Vellekson- University of Minnesota Table 1. Stand Accessment- MagPlots Table 2. Fall Planting Date x Seeding Rate-MagPlots Table 3. Fall Seeding Rates-MagPlots Table 4. Winter Hardiness Variety Trial-MagPlots Table 5. Overwintered Spring Wheat Control-Magnusson Farm Table 6. Fungicides Applications- D.Pieper and MagPlots Table 7. Growth Regulator x Fungicide Management-MagPlots Table 8. Late Growth Regulator Applications-MagPlots Table 9. Field Scale Fertility Trial-Magnusson Farm Table 10. Spring Topdress Fertility-Magnusson/Helmstetter Farms Table 11. Spring Fertility Applications-MagPlots Table 12. Fertility Source x Timing Applications-MagPlots Table 13. In Furrow Fertility to Late Fall Ryegrass Planting-MagPlots

Table 1. 2010 Perennial Ryegrass Stand Modification/Accessment Trial Magnusson Research Farm Roseau,Mn Fall Seeded Variety Arctic Green into wheat stubble Stand* Treatment Seed Yield (%squares w/plants) (#/ac.) 15% 838 33% 1010 50% 1118 100% 1186 LSD @5% level 299 * 1 2 remaining plants in each 6" x 7.5" grid square; all other plants hand pulled 100% stand is natural solid stand in 7.5" rows 4 2.5' x 2.5' grids per plot established 4/9/2010. 3 replications Harvest Date 7/29/10 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 2009 Ryegrass stand evaluation Richard Magnusson Farm Quest' Perennial ryegrass 1.5 mi. west of Roseau Natural stand using grid frame Seed Yield Stand (#/ac.) 39% 553 56% 1048 73% 1066

Table 2. 2009 Planting Date/Seeding Rate of Fall Planted Perennial Ryegrass Magnusson Research Farm Roseau,Mn. Seeding Seeding Seed Yield Maturity Lodging Date Rate (#/ac.) Date 7/22/2010 13-Jul 5 1368 26 Jul 5.0 13-Jul 8 1371 25 Jul 3.7 27-Jul 5 1502 25 Jul 5.3 27-Jul 8 1481 26 Jul 5.0 11-Aug 5 1501 23 Jul 5.0 11-Aug 8 1472 23 Jul 6.7 27-Aug 5 1543 22 Jul 5.3 27-Aug 8 1475 22 Jul 6.7 3-Sep 5 1487 24 Jul 7.7 3-Sep 8 1478 25 Jul 8.0 10-Sep 5 833 27 Jul 4.0 10-Sep 8 764 26 Jul 4.3 17-Sep 5 1273 27 Jul 6.0 17-Sep 8 1299 27 Jul 7.0 24-Sep 5 630 2 Aug 1.0 24-Sep 8 821 1 Aug 1.3 LSD @5% level 326 2 2.1 seeding rate 5#/acre=Mean yield 1267#/ac. seeding rate 8#/acre=Mean yield 1270#/ac. Granite spring wheat planted @ 20#/ac. In all plots Arctic Green Per.ryegrass seeded at 5# or 8#/ac. 57+31+40+8s to all 10/09 50+0+0 applied 4/23/10 Experimental Design: RCB with 3 reps Plot size= 6' x 15' XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Table 3. 2009 'Arctic Green' Perennial Rygrass Seeded at 3 Rates with 'Granite' Spring Wheat Cover * Seeding Rate Seed Yield (#/ac.) (#/ac.) 3# 1028 5# 970 8# 943 LSD @5% level NS * Granite spring wheat planted at 20#/ac. into summer fallow black ground Experimental design= RCB with 4 reps Seeding Date 9/14/2009

Table 4. 2009 Perennial Ryegrass Winter Hardiness Variety Trial St.Paul,Mn seeded 9/14/09 Roseau seeded 9/3/09 Winter Injury* St.Paul Roseau Variety seed lot 4/20/2010 4/24/2010 Affinity 3500 1 1 Arctic Green(MHT) 3900 1.1 1 Brightstar SLT 3661 1.3 1 FTM blue c1 09 3914 1 1 FTM red c1 08 3915 1 1 FTM white c1 09 3916 1.5 1 NK 200 3538 1.3 1 Quebec 3913 1 1 Ragnar II (P201) 3611 1 1 Ribeye(annual) 3689 5 3.8 Spreader III x P201 3910 1 1 Survivor 3848 1 1 WH x TQ(Polar Green) 3372 1 1 MSP comp. Watkins 3912 1.8 1 LSD @5% level 0.6 0.6 *Winter Injury 1=no injury;9=dead Experimental design=rcb with 4 reps Plots =1' x 20'

Table 5. Control of Overwintered Spring Wheat in Non Assure Tolerant Perennial Ryegrass Magnusson Farm Just southwest of Magnusson Research Farm Roseau,Mn Yield* Crop** Wheat** Treatment Adjuvunt (#/ac.) Injury Control Ht.(in.) 1 Callisto 3 oz.+coc NH* 3 43 25 2 2,4 DE+Nortron 1pt+2pt 785 0 57 24.7 3 Nortron 2 pt. NH* 0 50 25 4 Nortron 2pt. NH* 0 43 25 5 2,4 D E+Nortron.5pt+2pt. NH* 3 43 24 6 check* 633 0 0 25 7 2,4 D Ester+Callisto.5 pt.+3oz.+mso+28%n 871 0 83 24.3 8 2,4 D Ester+Callisto.5pt.+3oz.+MSO NH* 0 63 24.3 9 Callisto 3 oz.+mso+28%n 785 2 85 24 10 Callisto 3oz.+MSO 657 0 27 23.7 11 Callisto 5oz.+MSO NH* 0 60 23.7 12 Callisto 5oz.+MSO+28% NH* 7 90 24 13 Sharpen 2oz+MSO 654 13 47 23 14 Sharpen 1oz+MSO NH* 0 20 24 15 Sharpen 1oz+NIS NH* 0 20 24.3 128 9 22 1.3 * Harvested only selected plot treatments **Crop injury and wheat control 6/19/10 wheat in milk stage treatments 1,2 &3 applied 4/11/10 temp 52f wind 5 10 nw ryegrass 1/4" 1/2" All other Treatments 4/23/10 9am wind 5 12SW 50F ryegrass 3" wheat 7" Plot size= 10' x 30' Adjuvunt solution rates: MSO=1% COC= 1% NIS=.25% 28%N=2.5%

Table 6. 2010 Fungicide Application to 'Arctic Green' Perennial Ryegrass Locations=Dan Pieper Lake of the Woods Magnusson Research Farm 2 Location Pieper location only Pieper MagPlots mean Rust* Color** Lodging*** Treatment Rate Seed Yield (#/ac) 6/30/2010 7/21/201 7/21/2010 1) Folicur **** 4 oz. 663 NA NA 4 1.3 5.7 2)Folicur 4 oz.+.25%nis 740 1014 877 3.3 2.7 7 3)Tilt 4 oz. 693 880 787 3 2 5.3 4)Quilt 12oz. 907 1088 998 3 4.3 6.7 5)No Treatment 226 951 589 6 1 4 6)Absolute 6oz.+.5%HCCOC 1094 1103 1099 2 6.3 4 LSD @5% level 238 202 167 1.6 0.9 NS Location 1 = Dan Pieper Farm Sandy Shores,Lake of the Woods Location 2= Magnusson Research Farm *Rust 1=least;9=worst **Color 1=green;9=brown visual rating ***Lodging 1=erect;9=flat **** Folicur with no adjuvunt Pieper only Pieper Applications 6/22/10. Harvested 7/21/10 cldy wind 0 5 ese 65F Heavy pollen shedding MagPlots Applications 6/24/10. Harvest date 7/29/10 All plots severely lodged no observed differences among treatments Active Trade name common name (#/gal.) Folicur tebuconazole 3.6# Tilt propiconazole 3.6# Quilt propiconazole+azoxystrobin 1.04#+.62# Absolute tebuconazole+triflozystrobin 2.18#+2.18#

Table 7. 2010 Fungicide x Growth Regulator Applied to Arctic Green Perennial Ryegrass Magnusson Research Farm Seed* Application Yield Lodging** Treatment Rate dates (#/ac.) at harvest 1 Quilt 12 oz. 6/29/2010 922 8.3 2 Quilt+Quilt 6 oz. + 6 oz. 6/4 + 6/22 856 8.0 3 Folicur 5 oz. 6/29/2010 931 8.0 4 Headline/ Tilt 4 oz. + 3 oz. 6/4 + 6/22 957 7.3 5 No treatment 845 8.3 6 Apogee /Folicur 6oz.+5oz. 6/4 + 6/29 1204 5.0 7 Apogee /Quilt 6oz.+12oz 6/4 + 6/29 1127 5.0 8 Apogee only no fungicide 6oz. 6/4/2010 1148 5.0 9 Apogee +Headline / Tilt 6oz+4oz/3oz 6/4 + 6/29 1100 5.0 10 Radiate /Quilt 2oz+12oz. 6/4 + 6/29 1002 8.3 11 Apogee /Quilt 6oz.+12oz. 6/14+ 6/29 1109 5.3 12 Apogee/Quilt 6oz.+12oz. 6/22+ 6/29 821 7.0 LSD @5% level 198 1.5 *Mean of Apogee treatments= 1138#/ac. Mean of Fungicide only treatments= 916#/ac. ** Lodging;1=none,9=flat 6/4/2010 ryegrass 12 to 16 inches tall, early heading Temp 69F, Wind SW 5 15 mph 6/14/2010, ryegrass begin of shedding pollen ryegrass 16 22" tall Temp 68 F, wind 0 5 mph NE 6/22/2010,ryegrass light pollen shedding ryegrass 20 24" tall 6/29/2010,ryegrass mostly pollenated early milk stage filling This date was the scout, rust application date Harvest Date=7/28/10 Experimental Design= RCB with 3 reps Treatments Common name Active adjuvant Apogee prohexadione 27.5%DF.25%NIS+ 2.5%N Quilt propiconazole+azoxystrobin 1.04#+.62# Folicur tebuconazole 3.6#.25%NIS Absolute tebuconazole+triflozystrobin 2.18#+2.18# 1%COC Tilt propiconazole 3.6# Headline pyraclostrobin 2.09# Radiate IBA+Cytokinetin.85%+.15%

Table 8. 2010 Late Apogee and Fungicide Applications Arctic Green Perennial Ryegrass Magnusson Research farm Seed Yield Lodging* Treatment Rate Date (#/ac.) at harvest 1 Apogee/Folicur 4 oz./5oz. 6/14+ 6/22 1029 5.3 2 Apogee/Folicur 6 oz./5oz. 6/14+ 6/22 776 3.7 3 Apogee/Folicur 8 oz./5oz. 6/14+ 6/22 928 2.7 4 No treatment 880 6.7 5 Apogee only 6 oz. 14 Jun 779 4.3 6 Folicur only 6 oz. 22 Jun 794 7.7 7 Apogee+Quilt 6+10 22 Jun 818 6.0 8 Apogee+Folicur 6+6 22 Jun 928 7.0 LSD 5% level NS 2.0 * Lodging; 1=none, 9=flat 6/14/2010, ryegrass begin of shedding pollen ryegrass 16 to 22 inches tall Temp 68 F, wind 0 5 mph NE 6/22/2010, light Pollen shed Ryegrass 20 24 inches tall Temp 77 F, wind South 5 10 mph Harvest Date=7/28/10 Experimental Design= RCB with 3 reps

Table 9. Large Plot Spring Urea and Foliar 'N' Applications To 'Tandem' Perennial Ryegrass Magnusson Farm - Roseau,Mn Corrected Total Clean clean seed nitrogen Seed Yield % moisture Test wt. Fertilizer applied* applied (#/ac) at harvest per bushel 50+30+30 10/09 only 50# 986 12.1 29.9 50+30+30 10/09 +25N 5/12 75# 979 14.2 29.1 50+30+30 10/09 +50N 5/12 100# 800 15.6 28.6 50+30+30 10/09 +50N 5/12+ foliar 6/30** 103# 1173 15.4 27.9 LSD at 5% level 136 3 0.8 *All plots received 50+30+30 in October 2009. **Foliar treatment=1 gallon/acre RSA gradual N 30-0-0 (3# nitrogen) 100% headed - pollen shedding Experimental Design= RCB with 3 replications(12 plots) Plot size= 25' x 500'(.29acres) - Entire plot swathed and combined separately by Magnusson Farms Sub samples taken from each plot to determine %clean seed,moisture and test wt. XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Table 10. 2010 Top Dressing of Nitrogen Fertilizer to Perennial Ryegrass At 2 On Farm Locations Spring Additional spring* Treatment Nitrogen Applied Mag Helm Mag Helm Mag Helm Mag Helm Date Mag Helm Seed yield (#/ac.) Color ** Lodging*** Ht.(in.) 19 Apr 50 90 1211 1659 3.0 4.5 7.0 7.3 26.3 24.8 6 May 50 90 1128 1679 3.0 5.0 6.5 7.5 25.8 25.0 none added 0 0 885 1447 2.0 2.0 2.8 2.0 23.5 22.8 21 May 50 90 1177 1345 4.0 6.3 6.3 6.0 26.0 24.8 29 May 50 90 1193 1476 5.8 7.0 6.5 5.0 26.0 24.3 10 Jun 50 90 1171 1146 7.3 8.0 6.3 3.8 25.3 24.5 5/6+5/29 25+25 45+45 1251 1554 3.8 6.0 7.3 7.5 27.0 24.5 5/21+6/10 25+25 45+45 1166 1343 4.8 6.8 6.8 8.0 26.0 24.8 5/21+foliar**** 47+3 NA 1111 NA 4.3 NA 6.0 NA 25.5 NA LSD @5% level 113 213 1.4 1.3 1.5 1.7 1.3 1.2 * Urea source(46 0 0) ** Color,1=brown stubble; 9=green stubble ***Lodging,9=flat;1=no lodging ****Foliar apply during late heading 6/30/10- Magnusson site only Foliar treatment= 1 gallon/acre RSA Gradual N(30 0 0) 3# nitrogen Location Mag= Magnusson Farms just southwest of Magnusson Research Farm var. 'Tandem' 50+30+30 applied to all plots October 2009 Location Helm= Helmstetter Farm north of Roosevelt var. 'Arctic Green' 30+30+30 Applied to all plots October 2009 Both locations fall seeded into summer fallow/preventive plant

Table 11. 2009 10 Top Dress Fertility Arctic Green Perennial Ryegrass Magnusson Research Farm Urea source Spring N Liquid* Total # Spring Seed trt # 10/20/2009 Application foliar nitrogen application Yield Lodging Height Harvest Fertilizer Rate applications spring+fall timing (#/ac.) at harvest date 1 7+30+40 0 7 0 816 1.0 21.8 20 Jul 2 7+30+40 100# 107 10 May 1224 8.5 25.3 25 Jul 3 57+30+40 0 57 0 968 2.0 22 20 Jul 4 57+30+40 0 57 0 950 2.8 22 20 Jul 5 57+30+40 38# 4 x 3# 107 5/21/2010 1133 7.3 24.8 25 Jul 6 57+30+40 44# 2 x 3# 107 5/21/2010 1345 7.5 25.3 25 Jul 7 57+30+40 25# 82 5/10/2010 1133 2.5 23 21 Jul 8 57+30+40 50# 107 6/1/2010 1195 4.5 23 24 Jul 9 57+30+40 25# 82 6/1/2010 1099 3.8 23 21 Jul 10 57+30+40 50# 107 5/10/2010 1180 8.3 25.5 25 Jul 11 57+30+40 25#+25# 107 5/10+6/10 1218 7.5 25 25 Jul 12 57+30+40 50# 107 6/10/2010 1264 4.3 24.5 25 Jul 13 57+30+40 50# 107 6/22/2010 1162 2.3 23 23 Jul 14 57+30+40 25#+25# 107 5/10+6/10 1296 6.3 24.5 25 Jul 15 57+30+40 16+16+16 105 4/17+6/1+6/22 1247 7.3 25 25 Jul 16 57+30+40 16+16 1 x 15# 104 4/17+6/1+(6/22 liquid) 1291 6.3 24.8 25 Jul 17 57+30+40 47#+Npact 1 x 3# 107 5/10+(6/22 liquid) 1265 8.0 25.8 25 Jul 18 57+30+40 47#+BlackLabelZn 1 x 1# 105 5/10+(6/22 liquid) 1251 8.0 25.5 25 Jul LSD @5% level 222 2.0 1.6 2 All plots received 7+31+40 10/14/09 Ryegrass fall seeded into wheat stubble * Liquid foliar applications:(# of applications x # nitrogen/application) Treatment# 5 4 x 3# applications(28 0 0) timing=broadleaf,grass,growth regulator,and fungicide 6 2 x 3# applications(28 0 0) timing=broadleaf and fungicide 16 1 x 15# application(28 0 0) on 6/22 17 1 x 3# Nortrace N Pact (26 0 0) on 6/22 18 1 x 1# N BlackLabel ZN (6 20 0.77zn)on 6/22

Table 12. 2009 10 Fertility Source x Rate x Timing Perennial Ryegrass v.'arctic Green' spring planting under wheat Magnusson Research Farm Seed Application Yield Ht. Fertilizer Rate: timing* (#/ac.) at harvest 10 14 Oct 348 19.0 2 60+0+0 14 Oct 934 21.5 3 100+0+0 14 Oct 1135 24.0 4 50urea+50ESN 14 Oct 1148 23.3 5 100+0+0+22s 14 Oct 1175 23.5 6 140+0+0 14 Oct 1242 25.5 7 90urea+50ESN 14 Oct 1300 25.5 8 60+0+0 10/14+6/1** 789 22.0 9 100+0+0 10/14+6/1** 1135 23.0 10 (25+25ESN)+(25+25ESN) 10/14+6/1** 1086 22.3 11 100+0+0+22s 10/14+6/1** 1153 22.5 12 140+0+0 10/14+6/1** 1296 23.5 13 60+0+0 6 May 1110 23.5 14 100+0+0 6 May 1224 25.0 15 75+25ESN 6 May 1206 23.5 16 100+0+0+22s 6 May 1220 24.5 17 140+0+0 6 May 1371 24.8 18 60+0+0 10/14+4/14+6/1 1106 22.3 19 100+0+0 10/14+4/14+6/1 1231 25.5 20 (17+17ESN)+(17+17ESN)+33 10/14+4/14+6/1 1151 25.0 21 100+0+0+22s 10/14+4/14+6/1 1293 26.0 22 140+0+0 10/14+4/14+6/1 1331 25.8 LSD @5% level 155 1.5 All plots received 7+31+40 10/14/09 *Split fertility rates applied equally between fall and spring dates ** 6/1/10 applications later than scheduled Fertilizer used: 7+30+40 11 52 0(MAP) & 0 0 60) AMS 21 0 0 24 Urea 46 0 0 coated Urea(ESN) 44 0 0 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Table 13. 2009 Starter Fertilizer Applications to Late Planting of 'Arctic Green' Perennial Ryegrass Seeded 9/20/2009 Seed Yield Fertilizer rate (#/ac.) 1) 0 1324 2) 7+31+12 1002 3) 5+24+0 1084 4) 5+24+30 1074 LSD @5% level NS Ryegrass seeded in 3 6" rows/ plot x 4 replications 2 rows of spring wheat seeded between plots

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