ab Lung cancer tissue array, 75 cases, 150 samples (1.1mm), set 1

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ab178187 Lung cancer tissue array, 75 cases, 150 samples (1.1mm), set 1 Instructions for Use Designed for IHC or ISH based protein or RNA tissue profiling in lung cancers. This product is for research use only and is not intended for diagnostic use.

Product Overview: ab178187 is a lung cancer tissue array, non-overlapping with ab178188 or ab17818, 150 cores from normal/benign (5 cases) and cancer (70 cases with grading and TNM staging data) tissues in duplicates. Case: 70 Core: 150 Format: 10 rows x 15 columns Size: 1.1mm Thickness: 4µm All the tissues were fixed in 10% neutral buffered formalin for 24 hours and processed using identical SOPs. Sections were picked onto Superfrost Plus or Startfrost Adhesive slides. There may be >5% tissue core losses for a slide. But retention rate should be >90%. Notes for use: Bake the slides at 60 C for 30 minutes before use. If antigen retrieval is needed, it is always a good idea to start with a protocol with weak to mild strength. For hints and tips on Immunohistochemistry as well as other techniques please visit www.abcam.com/protocols. Keep up to date on Abcam events and conferences on Facebook & Twitter. 1

A01 59 M Lung Normal Normal A02 58 M Lung Normal Normal A03 66 M Lung Normal Normal A04 58 M Lung Inflammatory A05 64 M Lung Inflammatory A06 69 M Lung Malignant A07 69 M Lung Malignant A08 67 M Lung Malignant A09 60 M Lung Malignant A10 69 M Lung Metastasis A11 70 M Lung Malignant A12 60 M Lung Malignant A13 40 F Lung Malignant A14 48 M Lung Malignant Chronic pneumonia Chronic pneumonia cancers, from gastrointestional site Bronchioloalveolar I~ T3N0M0 A15 59 M Lung Malignant Adeno B01 59 M Lung Normal Normal B02 58 M Lung Normal Normal B03 66 M Lung Normal Normal B04 58 M Lung Inflammatory B05 64 M Lung Inflammatory B06 69 M Lung Malignant B07 69 M Lung Malignant B08 67 M Lung Malignant B09 60 M Lung Malignant Chronic pneumonia Chronic pneumonia I~ T3N0M0 2

B10 69 M Lung Metastasis B11 70 M Lung Malignant B12 60 M Lung Malignant B13 40 F Lung Malignant B14 48 M Lung Malignant cancers, from gastrointestional site Bronchioloalveolar B15 59 M Lung Malignant Adeno C01 49 M Lung Malignant Adeno ~ C02 47 F Lung Malignant Adeno I~ C03 50 M Lung Malignant C04 51 M Lung Malignant C05 45 M Lung Malignant Bronchioloalveolar I C06 55 F Lung Malignant Adeno C07 53 M Lung Malignant C08 43 M Lung Malignant C09 74 M Lung Malignant C10 77 M Lung Malignant C11 61 M Lung Metastasis C12 65 M Lung Malignant C13 53 M Lung Malignant C14 67 M Lung Malignant C15 63 M Lung Malignant adeno I~ I~ T3N1M0 D01 49 M Lung Malignant Adeno ~ D02 47 F Lung Malignant Adeno I~ 3

D03 50 M Lung Malignant D04 51 M Lung Malignant D05 45 M Lung Malignant Bronchioloalveolar I D06 55 F Lung Malignant Adeno D07 53 M Lung Malignant D08 43 M Lung Malignant D09 74 M Lung Malignant D10 77 M Lung Malignant D11 61 M Lung Metastasis D12 65 M Lung Malignant D13 53 M Lung Malignant D14 67 M Lung Malignant D15 63 M Lung Malignant E01 55 M Lung Malignant adeno I~ I~ T3N1M0 E02 42 F Lung Malignant Adeno T3N1M0 E03 74 M Lung Malignant Adeno T3N2M0 E04 40 F Lung Malignant E05 48 M Lung Malignant Adeno ~ E06 39 F Lung Malignant E07 47 M Lung Malignant E08 61 F Lung Metastasis E09 54 M Lung Malignant E10 66 F Lung Malignant adeno adeno E11 55 M Lung Malignant Adeno 4

E12 64 F Lung Malignant E13 55 M Lung Malignant E14 53 M Lung Malignant Adeno E15 64 F Lung Malignant F01 55 M Lung Malignant F02 42 F Lung Malignant Adeno T3N1M0 F03 74 M Lung Malignant Adeno T3N2M0 F04 40 F Lung Malignant F05 48 M Lung Malignant Adeno ~ F06 39 F Lung Malignant F07 47 M Lung Malignant F08 61 F Lung Metastasis F09 54 M Lung Malignant F10 66 F Lung Malignant adeno adeno F11 55 M Lung Malignant Adeno F12 64 F Lung Malignant F13 55 M Lung Malignant F14 53 M Lung Malignant Adeno F15 64 F Lung Malignant G01 57 M Lung Malignant G02 44 M Lung Malignant G03 53 M Lung Malignant G04 64 M Lung Metastasis G05 50 M Lung Malignant adeno ~ ~ 5

G06 65 M Lung Malignant G07 70 M Lung Malignant G08 78 M Lung Malignant G09 47 M Lung Malignant adeno I G10 46 M Lung Malignant Adeno G11 50 M Lung Malignant G12 44 M Lung Malignant G13 34 M Lung Malignant G14 52 F Lung Malignant Mucinous adeno G15 77 M Lung Malignant Adeno H01 57 M Lung Malignant H02 44 M Lung Malignant H03 53 M Lung Malignant H04 64 M Lung Metastasis H05 50 M Lung Malignant H06 65 M Lung Malignant H07 70 M Lung Malignant H08 78 M Lung Malignant H09 47 M Lung Malignant adeno adeno ~ ~ I H10 46 M Lung Malignant Adeno H11 50 M Lung Malignant H12 44 M Lung Malignant H13 34 M Lung Malignant H14 52 F Lung Malignant Mucinous adeno 6

H15 77 M Lung Malignant Adeno I01 55 F Lung Malignant Adeno I02 57 M Lung Malignant I I03 70 F Lung Malignant Adeno I04 64 F Lung Malignant Carcinoid T3N1M0 I05 70 F Lung Malignant Adeno I06 55 M Lung Malignant I07 60 F Lung Malignant I08 65 M Lung Malignant I09 56 M Lung Malignant I10 50 F Lung Malignant I11 67 M Lung Malignant I12 74 M Lung Malignant I13 78 M Lung Malignant I14 56 M Lung Malignant I15 56 M Lung Malignant adeno adeno ~ I~ ~ T3N0M0 J01 55 F Lung Malignant Adeno J02 57 M Lung Malignant I J03 70 F Lung Malignant Adeno J04 64 F Lung Malignant Carcinoid T3N1M0 J05 70 F Lung Malignant Adeno J06 55 M Lung Malignant J07 60 F Lung Malignant J08 65 M Lung Malignant J09 56 M Lung Malignant adeno ~ 7

J10 50 F Lung Malignant J11 67 M Lung Malignant J12 74 M Lung Malignant J13 78 M Lung Malignant J14 56 M Lung Malignant J15 56 M Lung Malignant adeno I~ ~ T3N0M0 8

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