InertSustain Phenylhexyl. A New Second Choice Column to Change Chromatographic Behavior

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InertSustain Phenylhexyl A ew Second Choice Column to Change Chromatographic Behavior

Providing Alternative Selectivity to C8 Phases InertSustain Phenylhexyl columns are bonded with phenylhexyl groups, which employs a phenyl ring with a hexyl (6- carbon) linker and is densely bonded to our newly developed ES silica gel delivering complementary selectivity to straight alkyl-chain columns, but with industry leading reproducibility, inertness and low back pressure. Physical Properties Silica : ES (Evolved Surface) Silica Gel Particle Size : μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume : 0.85 ml/g Bonded Phase : Phenylhexyl Groups End-capping : Complete Carbon Loading : 9.0 % USP Code : L ph Range :.0 to 0.0 Benefits Different selectivity to C8 phases Strong Retentivity than other Phenyl phases Highly inert packing material results in less tailing of peaks for virtually any type of analytes Deliver extreme sensitivity analysis in LC-MS and LC-MS/MS methods Extreme resistance to low and high ph mobile phases Excellent stability to 00 % aqueous mobile phases Compatible with The European Pharmacopeia (Ph. Eur.) methods Endlessly reproducible from column-to-column and batch-to-batch

Different Selectivity to C8 Phases The following selectivity test was used to understand the difference in selectivity between a conventional C8 phase, Phenylhexyl and Phenyl phases. Column Size : 5 μm, 50 x 4.6 mm I.D. Eluent : A) CH OH B) H O A/B = 70/0, v/v Flow Rate :.0 ml/min Col. Temp. :40 Detection :UV 54 nm Sample : 4 5, 6 7 InertSustain C8. Uracil. Caffeine. Phenol 4. n-butylbenzene 5. o-terphenyl 6. n-amylbenzene 7. Triphenylene 0 40 60 80 7 4 6 5 InertSustain Phenylhexyl Bonded Phase: Phenylhexyl Groups (C6-Ph) 0 0 0 7 4 6 5 InertSustain Phenyl Bonded Phase: Phenyl Groups (C0-Ph) 0 0 0 Sample o., Uracil cannot be retained in a reversed phase mode when using the mobile phase condition described on the right. Therefore, Uracil was used to determine the t0 position. t0: Void Volume Sample o.,, Caffeine and Phenol are used to confirm the amount of residual silanol on the surface of the silica gel. Caffeine elutes later against Phenol when there is a lot of residual silanol on the surface of the silica gel. Sample o.4, n-butylbenzene and Sample o.6, n-amylbenzene were used to determine the hydrophobic property of the column. n-amylbenzene elutes later against n-butylbenzene when the hydrophobicity of the column is high. Stereoselectivity is indicated by Sample o.5, o-terphenyl and Sample o.7, Triphenylene. o-terphenyl has a twisted tertiary structure and Triphenylene has a planar structure. Triphenylene elutes later against o-terphenyl when the stereoselectivity of the column is high.

Comparison of Selectivity Between a C8 and Phenylhexyl Phase Figure compares the selectivity provided by a conventional C8 and Phenylhexyl phase. InertSustain Phenylhexyl show stronger retention of compounds containing nitro groups. Figure compares the separation of analgesic drugs on a conventional C8 and Phenylhexyl phase. Indomethacin was strongly retained on InertSustain Phenylhexyl due to the presence of strong pi-pi interaction resulting in change in elution order with better separation. 0 0 0 40 50 InertSustain C8 Figure. Column Size : 5 μm, 50 x 4.6 mm I.D. Eluent : A) CH OH B) H O A/B = 0/70, v/v Flow Rate :.0 ml/min Col. Temp. : 40 Detection : UV 0 nm Sample :.,,5-Trinitrobenzene.,-Dinitrobenzene. itrobenzene O O O O O.,,5-Trinitrobenzene.,-Dinitrobenzene O 0 0 0 40 50 InertSustain Phenylhexyl. itrobenzene Analysis of Analgesic Drugs 0 0 0 40 InertSustain C8 Figure. Column Size : 5 μm, 50 x 4.6 mm I.D. Eluent : A) CH C B) 5 mm KH PO 4 (ph.0, H PO 4 ) A/B = 45/55, v/v Flow Rate :.0 ml/min Col. Temp. :40 Detection :UV 0 nm Sample :. Indomethacin. Ibuprofen O Cl H C O CH OH H C CH H CH O OH 0 0 0 40 InertSustain Phenylhexyl O. Indomethacin. Ibuprofen

Strong Retentivity than other Phenyl Phases The retentivity of InertSustain Phenylhexyl is stronger comparing with commercially available Phenyl columns. Sample o.5, n-amylbenzene was used to determine the hydrophobic property of columns. 4 5 0 0 0, InertSustain Phenylhexyl 6 6 7, Column Size : 5 μm, 50 x 4.6 mm I.D. Eluent : A) CH OH B) H O A/B = 70/0, v/v Flow Rate :.0 ml/min Col. Temp. :40 Detection :UV 54 nm Sample :. Uracil. Caffeine. Phenol 4. n-butylbenzene 5. n-amylbenzene 6 6. Triphenylene 7. o-terphenyl 4 5 7 4 5 7 0 0 0 XBridge Phenyl 0 0 0 40 Inertsil Ph, 6 4 5 6, 7 4 5 7 0 0 0, Luna Phenyl-Hexyl 0 0 0 40 6 InertSustain Phenyl 4 5 7 6 4 5 7 0 0 0 Zorbax Phenyl-Hexyl 0 0 0 40 Inertsil Ph-

Benefits of Highly Inert Packing Material InertSustain Phenylhexyl also maintains the same extreme inertness, wide ph range and provide separations with symmetric peaks. Poorly end-capped columns often show tailing of peaks due to the presence of silanols resulting in longer analysis time. Analysis of Antidepressants 4 Eluent Flow Rate Col. Temp. :40 Detection :UV 54 nm Sample :A) CH C B) 5 mm KH PO 4 (ph 7.0, K HPO 4 ) A/B = 50/50, v/v :.0 ml/min :. Imipramine hydrochloride. Amitriptyline hydrochloride. Clomipramine hydrochloride 4. Mianserin hydrochloride 0 0 InertSustain Phenylhexyl. Imipramine hydrochloride. Amitriptyline hydrochloride 4 H C Cl H 0 0 Luna Phenyl-Hexyl. Clomipramine hydrochloride 4. Mianserin hydrochloride

Extreme Sensitivity Analysis in LC-MS and LC-MS/MS Methods The usage of highly inert packing material creates another benefit for LC-MS and LC-MS/MS applications. As shown below, InertSustain Phenylhexyl deliver excellent peak shape and high sensitivity even with strong basic analyte. H CO H Dextromethorphan CH System Column Eluent : GL-7700 4000 QTRAP : μm, 50 x. mm I.D. : A) CH C B) 0 mm CH COOH 4 in H O A/B = 0/70 (gradient mixer ), v/v :0 μl/min Flow rate Col. Temp. :40 Detection :SIM (ESI, Positive ) CUR IS TEM GS GS ihe 0 5500 500 60 40 O Injection Vol. Sample : μl :Dextromethorphan (50 ng/ml).0e6.0e6.0e6 Max..e6 cps..0e6 Max..7e6 cps..0e6.0e6 0.0 4 6 8 0 4 6 8 0.0 4 6 8 0 4 6 8 InertSustain Phenylhexyl Other Brands Phenyl Column

Wide ph Compatibility with Long Column Lifetime As shown in the experiment below, InertSustain Phenylhexyl maintained high efficiency for 0 hours. % Initial 00 80 60 40 0 0 50 00 50 0 Purging Column :5 μm, 50 4.6 mm I.D. Eluent :A) 50 mm Triethylamine (ph 9.5) B)CH OH A/B = 70/0, v/v Flow Rate :.0 ml/min Col. Temp. :50 Eluent :CH C/H O = 65/5, v/v Flow Rate :.0 ml/min Col. Temp. :40 Detection :UV 54 nm Sample :aphthalene Hours of Purging (h) Excellent Stability to 00 % Aqueous Mobile Phases When analyzing hydrophilic compounds under water rich mobile phase condition, once the pump is stopped, the hydrophobic bonded group pushes the aqueous mobile phase out off the pore in an irreversible fashion, in what has become known as the dewetting phenomenon. As shown in the following test, InertSustain Phenylhexyl demonstrates excellent stability to dewetting guaranteeing highly stable, reliable and reproducible chromatograms. 4 First Injection 5 0 4 6 8 0 Dewetting Test Column : 5 μm, 50 x 4.6 mm I.D. Eluent : 00 % H O Flow rate :.0 ml/min Col. Temp. : 40 Detection : UV 54 nm Sample :.Cytosine.Uracil.Guanine 4.Thymine 5.Adenine 0 4 6 8 0 InertSustain Phenylhexyl Second Injection Variation: 99.89 % Testing Procedure ) 00 % water is introduced into column over 60 minutes. ) Conduct analysis. ) Stop flow for 5 minutes. 4) 00 % water is introduced again into column over 0 minutes. 5) Stop flow for 5 minutes again. 6) Conduct analysis.

Compatible with The European Pharmacopeia (Ph. Eur.) Methods Analysis of Indomethacin H C O O CH OH O Indomethacin Cl Column : μm, 50 x 4.6 mm I.D. Eluent : A) 0 g/l CH COOH in H O B) CH C A/B = 70/0 min - 70/0 9 min - 50/50 min 50/50-0 min - 70/0 9 min - 0/70 6 min - 0/70, v/v Flow Rate :.0 ml/min Col. Temp. : 40 Detection : UV 54 nm Injection Vol. : μl Criteria : Indomethacin to be eluted approx. 8 min 0 0 InertSustain Phenylhexyl Access to the latest pharmaceutical, life science, environmental and food applications at www.glsciences.com/tech/inertsearch

Comparison of Performance By conducting the tests below, we can evaluate the degree of inertness of the column. To strictly evaluate all columns, the below mentioned samples were used, which are all known as strong adsorptive compounds. Strong Basic Compound Test Dextromethorphan and Berberine Chloride are strong basic compounds. Severe tailing can be confirmed when the packing material contains residual silanol groups. H CO Column : 5 μm, 50 x 4.6 mm I.D. Eluent : A) CH C B) 5 mm K HPO 4 (ph 7.0, KH PO 4 ) A/B = 40/60,v/v Flow Rate :.0 ml/min Col. Temp. : 40 Detection : UV 0 nm Injection Vol : μl Sample : : Dextromethorphan H CH :Dextromethorphan Strong Acidic Compound Test Brilliant Blue FCF has three sulfonic groups in its chemical structure, severe tailing will occur when the surface of the packing material is slightly basic. - O S SO - SO - Column : 5 μm, 50 x 4.6 mm I.D. Eluent : A) CH C B) 0. % H PO 4 A/B = 5/75,v/v Flow Rate :.0 ml/min Col. Temp. : 40 Detection : UV 54 nm Injection Vol : 5 μl Sample : :Brilliant Blue FCF :Phenol :Salicylic acid + :Brilliant Blue FCF

Comparison of Performance Strong Basic Compound Test Strong Acidic Compound Test InertSustain Phenylhexyl : Dextromethorphan : Brilliant Blue FCF : Phenol : Salicylic acid 0 0 0 0 Luna Phenyl-Hexyl. o peak 0 0 0 0 XBridge Phenyl. o peak 0 0 0 0 Zorbax Phenyl-Hexyl. o peak 0 0 0 0 0 40

Analytical Columns 50-899 InertSustain Phenylhexyl HP µm.x0mm 50 50-890 InertSustain Phenylhexyl HP µm.x50mm 50 50-89 InertSustain Phenylhexyl HP µm.x75mm 50 50-89 InertSustain Phenylhexyl HP µm.x00mm 50 50-89 InertSustain Phenylhexyl HP µm.x50mm 50 50-894 InertSustain Phenylhexyl HP µm.x50mm 50 50-895 InertSustain Phenylhexyl HP µm.0x0mm 50 50-896 InertSustain Phenylhexyl HP µm.0x50mm 50 50-897 InertSustain Phenylhexyl HP µm.0x75mm 50 50-898 InertSustain Phenylhexyl HP µm.0x00mm 50 50-899 InertSustain Phenylhexyl HP µm.0x50mm 50 50-89 InertSustain Phenylhexyl HP µm.0x50mm 50 50-89 InertSustain Phenylhexyl HP µm 4.6x0mm 50 50-89 InertSustain Phenylhexyl HP µm 4.6x50mm 50 50-89 InertSustain Phenylhexyl HP µm 4.6x75mm 50 50-894 InertSustain Phenylhexyl HP µm 4.6x00mm 50 50-895 InertSustain Phenylhexyl HP µm 4.6x50mm 50 50-896 InertSustain Phenylhexyl HP µm 4.6x50mm 50 * End-fittings are /6 Waters-compatible.

Analytical Columns 50-8960 InertSustain Phenylhexyl µm.0x0mm 50-896 InertSustain Phenylhexyl µm.0x50mm 50-896 InertSustain Phenylhexyl µm.0x75mm 50-896 InertSustain Phenylhexyl µm.0x00mm 50-8964 InertSustain Phenylhexyl µm.0x50mm 50-8965 InertSustain Phenylhexyl µm.0x50mm 50-8966 InertSustain Phenylhexyl µm.5x0mm 50-8967 InertSustain Phenylhexyl µm.5x50mm 50-8968 InertSustain Phenylhexyl µm.5x75mm 50-8969 InertSustain Phenylhexyl µm.5x00mm 50-8970 InertSustain Phenylhexyl µm.5x50mm 50-897 InertSustain Phenylhexyl µm.5x50mm 50-894 InertSustain Phenylhexyl µm.x0mm 50-895 InertSustain Phenylhexyl µm.x50mm 50-896 InertSustain Phenylhexyl µm.x75mm 50-897 InertSustain Phenylhexyl µm.x00mm 50-898 InertSustain Phenylhexyl µm.x50mm 50-899 InertSustain Phenylhexyl µm.x50mm 50-89 InertSustain Phenylhexyl µm.0x0mm 50-89 InertSustain Phenylhexyl µm.0x50mm 50-89 InertSustain Phenylhexyl µm.0x75mm 50-894 InertSustain Phenylhexyl µm.0x00mm 50-895 InertSustain Phenylhexyl µm.0x50mm 50-896 InertSustain Phenylhexyl µm.0x50mm 50-898 InertSustain Phenylhexyl µm 4.0x0mm 50-899 InertSustain Phenylhexyl µm 4.0x50mm 50-8940 InertSustain Phenylhexyl µm 4.0x75mm 50-894 InertSustain Phenylhexyl µm 4.0x00mm 50-894 InertSustain Phenylhexyl µm 4.0x50mm 50-894 InertSustain Phenylhexyl µm 4.0x50mm 50-8945 InertSustain Phenylhexyl µm 4.6x0mm 50-8946 InertSustain Phenylhexyl µm 4.6x50mm 50-8947 InertSustain Phenylhexyl µm 4.6x75mm 50-8948 InertSustain Phenylhexyl µm 4.6x00mm 50-8949 InertSustain Phenylhexyl µm 4.6x50mm 50-8950 InertSustain Phenylhexyl µm 4.6x50mm * End-fittings are /6 Waters-compatible.

Analytical Columns 50-8908 InertSustain Phenylhexyl 5 µm.0x0mm 50-8909 InertSustain Phenylhexyl 5 µm.0x50mm 50-89040 InertSustain Phenylhexyl 5 µm.0x75mm 50-8904 InertSustain Phenylhexyl 5 µm.0x00mm 50-8904 InertSustain Phenylhexyl 5 µm.0x50mm 50-8904 InertSustain Phenylhexyl 5 µm.0x50mm 50-89044 InertSustain Phenylhexyl 5 µm.5x0mm 50-89045 InertSustain Phenylhexyl 5 µm.5x50mm 50-89046 InertSustain Phenylhexyl 5 µm.5x75mm 50-89047 InertSustain Phenylhexyl 5 µm.5x00mm 50-89048 InertSustain Phenylhexyl 5 µm.5x50mm 50-89049 InertSustain Phenylhexyl 5 µm.5x50mm 50-8900 InertSustain Phenylhexyl 5 µm.x0mm 50-8900 InertSustain Phenylhexyl 5 µm.x50mm 50-8900 InertSustain Phenylhexyl 5 µm.x75mm 50-89004 InertSustain Phenylhexyl 5 µm.x00mm 50-89005 InertSustain Phenylhexyl 5 µm.x50mm 50-89006 InertSustain Phenylhexyl 5 µm.x50mm 50-89008 InertSustain Phenylhexyl 5 µm.0x0mm 50-89009 InertSustain Phenylhexyl 5 µm.0x50mm 50-8900 InertSustain Phenylhexyl 5 µm.0x75mm 50-890 InertSustain Phenylhexyl 5 µm.0x00mm 50-890 InertSustain Phenylhexyl 5 µm.0x50mm 50-890 InertSustain Phenylhexyl 5 µm.0x50mm 50-8905 InertSustain Phenylhexyl 5 µm 4.0x0mm 50-8906 InertSustain Phenylhexyl 5 µm 4.0x50mm 50-8907 InertSustain Phenylhexyl 5 µm 4.0x75mm 50-8908 InertSustain Phenylhexyl 5 µm 4.0x00mm 50-8909 InertSustain Phenylhexyl 5 µm 4.0x50mm 50-890 InertSustain Phenylhexyl 5 µm 4.0x50mm 50-890 InertSustain Phenylhexyl 5 µm 4.6x0mm 50-890 InertSustain Phenylhexyl 5 µm 4.6x50mm 50-8904 InertSustain Phenylhexyl 5 µm 4.6x75mm 50-8905 InertSustain Phenylhexyl 5 µm 4.6x00mm 50-8906 InertSustain Phenylhexyl 5 µm 4.6x50mm 50-8907 InertSustain Phenylhexyl 5 µm 4.6x50mm * End-fittings are /6 Waters-compatible.

Guard Columns for UHPLC 50-0860 Holder for Guard Columns for UHPLC - Guard Columns for UHPLC, Holder/Cartridge Set, 50-890 Holder with pcs.5x0 mm Cartridges, InertSustain Phenylhexyl µm 80 Guard Columns for UHPLC, Holder/Cartridge Set, 50-89 Holder with pcs.x0 mm Cartridges, InertSustain Phenylhexyl µm 80 Guard Columns for UHPLC, Holder/Cartridge Set, 50-89 Holder with pcs.0x0 mm Cartridges, InertSustain Phenylhexyl µm 80 50-897 Guard Columns for UHPLC,.5x0 mm, pcs, InertSustain Phenylhexyl µm 80 50-898 Guard Columns for UHPLC,.x0 mm, pcs, InertSustain Phenylhexyl µm 80 50-899 Guard Columns for UHPLC,.0x0 mm, InertSustain Phenylhexyl µm 80 Cartridge Guard Column Ei (on-metal) 50-08650 Holder for 0 mm, Cartridge Guard Column Ei - Cartridge Ei Holder/Cartridge Set, 50-89 Holder with pcs.0x0 mm, InertSustain Phenylhexyl 5 µm Cartridge Ei Holder/Cartridge Set, 50-898 Holder with pcs.x0 mm, InertSustain Phenylhexyl 5 µm Cartridge Ei Holder/Cartridge Set, 50-896 Holder with pcs.0x0 mm, InertSustain Phenylhexyl 5 µm Cartridge Ei Holder/Cartridge Set, 50-894 Holder with pcs 4.0x0 mm, InertSustain Phenylhexyl 5 µm 50-899 Cartridge Ei Replacement Cartridges, pcs.0x0 mm, InertSustain Phenylhexyl 5 µm 50-897 Cartridge Ei Replacement Cartridges, pcs.x0 mm, InertSustain Phenylhexyl 5 µm 50-895 Cartridge Ei Replacement Cartridges, pcs.0x0 mm, InertSustain Phenylhexyl 5 µm 50-89 Cartridge Ei Replacement Cartridges, pcs 4.0x0 mm, InertSustain Phenylhexyl 5 µm * End-fittings are /6 Waters-compatible.

Cartridge Guard Column E 50-08500 Holder for 0 mm, Cartridge Guard Column E - 50-08550 Holder for mm, Cartridge Guard Column E - 50-890 Holder with pcs.0x0 mm Cartridges, InertSustain Phenylhexyl µm 50-89 Holder with pcs.5x0 mm Cartridges, InertSustain Phenylhexyl µm 50-8998 Holder with pcs.0x0 mm Cartridges, InertSustain Phenylhexyl µm 50-8996 Holder with pcs 4.0x0 mm Cartridges, InertSustain Phenylhexyl µm 50-896 Holder with pcs.0x mm Cartridges, InertSustain Phenylhexyl µm 50-894 Holder with pcs 4.0x mm Cartridges, InertSustain Phenylhexyl µm 50-8906 Holder with pcs.0x0 mm Cartridges, InertSustain Phenylhexyl 5 µm 50-8908 Holder with pcs.5x0 mm Cartridges, InertSustain Phenylhexyl 5 µm 50-8904 Holder with pcs.0x0 mm Cartridges, InertSustain Phenylhexyl 5 µm 50-890 Holder with pcs 4.0x0 mm Cartridges, InertSustain Phenylhexyl 5 µm 50-89 Holder with pcs.0x mm Cartridges, InertSustain Phenylhexyl 5 µm 50-890 Holder with pcs 4.0x mm Cartridges, InertSustain Phenylhexyl 5 µm 50-8999 Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustain Phenylhexyl µm 50-89 Cartridge E Replacement Cartridges, pcs.5x0mm, InertSustain Phenylhexyl µm 50-8997 Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustain Phenylhexyl µm 50-8995 Cartridge E Replacement Cartridges, pcs 4.0x0mm, InertSustain Phenylhexyl µm 50-895 Cartridge E Replacement Cartridges, pcs.0xmm, InertSustain Phenylhexyl µm 50-89 Cartridge E Replacement Cartridges, pcs 4.0xmm, InertSustain Phenylhexyl µm 50-8905 Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustain Phenylhexyl 5 µm 50-8907 Cartridge E Replacement Cartridges, pcs.5x0mm, InertSustain Phenylhexyl 5 µm 50-890 Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustain Phenylhexyl 5 µm 50-890 Cartridge E Replacement Cartridges, pcs 4.0x0mm, InertSustain Phenylhexyl 5 µm 50-89 Cartridge E Replacement Cartridges, pcs.0xmm, InertSustain Phenylhexyl 5 µm 50-8909 Cartridge E Replacement Cartridges, pcs 4.0xmm, InertSustain Phenylhexyl 5 µm * End-fittings are /6 Waters-compatible.

GL Cart Guard Column * End-fittings are /6 Waters-compatible. 50-0870 Holder for GL Cart Multipurpose Type - GL Cart Holder/Cartridge Set, 50-898 Holder with 5 pcs Cartridges, InertSustain Phenylhexyl µm 50-897 GL Cart Replacement Cartridges, 0 pcs 4.6x5 mm, InertSustain Phenylhexyl µm GL Cart Holder/Cartridge Set, 50-89 Holder with 5 pcs Cartridges, InertSustain Phenylhexyl 5 µm 50-89 GL Cart Replacement Cartridges, 0 pcs 4.6x5 mm, InertSustain Phenylhexyl 5 µm Conventional Guard Column 50-8975 Guard Column.0Xmm InertSustain Phenylhexyl µm 50-897 Guard Column.0X50mm InertSustain Phenylhexyl µm 50-8974 Guard Column.5Xmm InertSustain Phenylhexyl µm 50-897 Guard Column.5X50mm InertSustain Phenylhexyl µm 50-8959 Guard Column.Xmm InertSustain Phenylhexyl µm 50-8954 Guard Column.X50mm InertSustain Phenylhexyl µm 50-8958 Guard Column.0Xmm InertSustain Phenylhexyl µm 50-895 Guard Column.0X50mm InertSustain Phenylhexyl µm 50-8955 Guard Column 4.0X0mm InertSustain Phenylhexyl µm 50-8957 Guard Column 4.0Xmm InertSustain Phenylhexyl µm 50-895 Guard Column 4.0X50mm InertSustain Phenylhexyl µm 50-8956 Guard Column 4.6Xmm InertSustain Phenylhexyl µm 50-895 Guard Column 4.6X50mm InertSustain Phenylhexyl µm 50-8905 Guard Column.0Xmm InertSustain Phenylhexyl 5 µm 50-8905 Guard Column.0X50mm InertSustain Phenylhexyl 5 µm 50-8905 Guard Column.5Xmm InertSustain Phenylhexyl 5 µm 50-89050 Guard Column.5X50mm InertSustain Phenylhexyl 5 µm 50-8907 Guard Column.Xmm InertSustain Phenylhexyl 5 µm 50-890 Guard Column.X50mm InertSustain Phenylhexyl 5 µm 50-8906 Guard Column.0Xmm InertSustain Phenylhexyl 5 µm 50-890 Guard Column.0X50mm InertSustain Phenylhexyl 5 µm 50-890 Guard Column 4.0X0mm InertSustain Phenylhexyl 5 µm 50-8905 Guard Column 4.0Xmm InertSustain Phenylhexyl 5 µm 50-8900 Guard Column 4.0X50mm InertSustain Phenylhexyl 5 µm 50-8904 Guard Column 4.6Xmm InertSustain Phenylhexyl 5 µm 50-8909 Guard Column 4.6X50mm InertSustain Phenylhexyl 5 µm * End-fittings are /6 Waters-compatible.

Preparative Columns Max. Operating Pressure 50-89054 InertSustain Phenylhexyl 5 µm 6.0x50mm 50-89055 InertSustain Phenylhexyl 5 µm 6.0x00mm 50-89056 InertSustain Phenylhexyl 5 µm 6.0x50mm 50-89057 InertSustain Phenylhexyl 5 µm 6.0x50mm 50-89058 InertSustain Phenylhexyl 5 µm 7.6x50mm 50-89059 InertSustain Phenylhexyl 5 µm 7.6x00mm 50-89060 InertSustain Phenylhexyl 5 µm 7.6x50mm 50-8906 InertSustain Phenylhexyl 5 µm 7.6x50mm 50-8906 InertSustain Phenylhexyl 5 µm 0x50mm 50-8906 InertSustain Phenylhexyl 5 µm 0x00mm 50-89064 InertSustain Phenylhexyl 5 µm 0x50mm 50-89065 InertSustain Phenylhexyl 5 µm 0x50mm 50-89066 InertSustain Phenylhexyl 5 µm 4x50mm 50-89067 InertSustain Phenylhexyl 5 µm 4x00mm 50-89068 InertSustain Phenylhexyl 5 µm 4x50mm 50-89069 InertSustain Phenylhexyl 5 µm 4x50mm 50-89070 InertSustain Phenylhexyl 5 µm x50mm 50-8907 InertSustain Phenylhexyl 5 µm x00mm 50-8907 InertSustain Phenylhexyl 5 µm x50mm 50-8907 InertSustain Phenylhexyl 5 µm x50mm * End-fittings are /6 Waters-compatible. Guard Columns for Preparative Columns * End-fittings are /6 Waters-compatible. 50-89074 InertSustain Phenylhexyl 5 µm 6.0x50mm Guard 50-89074 InertSustain Phenylhexyl 5 µm 7.6x50mm Guard 50-89074 InertSustain Phenylhexyl 5 µm 0x50mm Guard 50-89074 InertSustain Phenylhexyl 5 µm 4x50mm Guard 50-89074 InertSustain Phenylhexyl 5 µm x50mm Guard Cartridge Guard Columns for Preparative Columns 50-069 Holder for Prep Guard Cartridge - 50-89079 Prep Guard Cartridges, pcs 7.6x0 mm, InertSustain Phenylhexyl 5 µm Prep Guard Holder/Cartridge Set, 50-89080 Holder with pcs 7.6x0 mm Cartridges, InertSustain Phenylhexyl 5 µm * End-fittings are /6 Waters-compatible.

Capillary Columns 50-898 Capillary EX-AO 0.05X50mm InertSustain Phenylhexyl µm 5 50-8984 Capillary EX-AO 0.05X50mm InertSustain Phenylhexyl µm 5 50-8985 Capillary EX-AO 0.05X50mm InertSustain Phenylhexyl µm 5 50-8986 Capillary EX-AO 0.075X50mm InertSustain Phenylhexyl µm 5 50-8987 Capillary EX-AO 0.075X50mm InertSustain Phenylhexyl µm 5 50-8990 Capillary EX-AO 0.X50mm InertSustain Phenylhexyl µm 5 50-899 Capillary EX-AO 0.X50mm InertSustain Phenylhexyl µm 5 50-899 Capillary EX-AO 0.X50mm InertSustain Phenylhexyl µm 5 50-899 Capillary EX-AO 0.X50mm InertSustain Phenylhexyl µm 5 50-8994 Capillary EX-AO 0.X50mm InertSustain Phenylhexyl µm 5 50-8976 Capillary EX 0.X50mm InertSustain Phenylhexyl µm 50-8977 Capillary EX 0.X50mm InertSustain Phenylhexyl µm 50-8978 Capillary EX 0.5X50mm InertSustain Phenylhexyl µm 50-8979 Capillary EX 0.5X50mm InertSustain Phenylhexyl µm 50-8980 Capillary EX 0.7X50mm InertSustain Phenylhexyl µm 50-898 Capillary EX 0.7X50mm InertSustain Phenylhexyl µm 50-898 EX Micro Guard (Metal) 0.Xmm InertSustain Phenylhexyl µm 50-89089 Capillary EX-AO 0.05x50mm InertSustain Phenylhexyl 5 µm 5 50-89090 Capillary EX-AO 0.05x50mm InertSustain Phenylhexyl 5 µm 5 50-8909 Capillary EX-AO 0.05x50mm InertSustain Phenylhexyl 5 µm 5 50-8909 Capillary EX-AO 0.075x50mm InertSustain Phenylhexyl 5 µm 5 50-8909 Capillary EX-AO 0.075x50mm InertSustain Phenylhexyl 5 µm 5 50-89094 Capillary EX-AO 0.075x50mm InertSustain Phenylhexyl 5 µm 5 50-89097 Capillary EX-AO 0.x50mm InertSustain Phenylhexyl 5 µm 5 50-89098 Capillary EX-AO 0.x50mm InertSustain Phenylhexyl 5 µm 5 50-89099 Capillary EX-AO 0.x50mm InertSustain Phenylhexyl 5 µm 5 50-8900 Capillary EX-AO 0.x50mm InertSustain Phenylhexyl 5 µm 5 50-8908 Capillary EX 0.x50mm InertSustain Phenylhexyl 5 µm 50-8908 Capillary EX 0.x50mm InertSustain Phenylhexyl 5 µm 50-8908 Capillary EX 0.5x50mm InertSustain Phenylhexyl 5 µm 50-89084 Capillary EX 0.5x50mm InertSustain Phenylhexyl 5 µm 50-89085 Capillary EX 0.7x50mm InertSustain Phenylhexyl 5 µm 50-89086 Capillary EX 0.7x50mm InertSustain Phenylhexyl 5 µm 50-89087 EX Micro Guard (Metal) 0.xmm InertSustain Phenylhexyl 5 µm 50-89088 EX Micro Guard (on-metal) 0.xmm InertSustain Phenylhexyl 5 µm * End-fittings are Valco /6 (0- UF). * Valco / (6-40 UF) end-fittings are available, indicate / when ordering.

Worldwide Ordering Information GL Sciences, Inc. USA 47 Torrance Blvd. Suite 55 Torrance, CA 9050 Phone: 0-65-444 Fax: 0-65-445 Email: info@glsciencesinc.com Web: www.glsciencesinc.com GL Sciences, Inc. Japan - ishishinjuku 6-Chome Shinjuku-ku, Tokyo, 6-0, Japan Phone: +8--5-66 Fax: +8--5-66 Email: world@gls.co.jp Web: www.glsciences.com GL Sciences B.V. De Sleutel 9 565 AS Eindhoven The etherlands Phone: + (0)40 54 95 Fax: + (0)40 54 97 79 Email: info@glsciences.eu Web: www.glsciences.eu International Distributors Visit our Website at www.glsciences.com/distributors The GL Sciences name, the GL Sciences logo and the following registered trademark or trademark are the property of GL Sciences Inc. Inertsil InertSustain InertSustainSwift All other trademarks or service marks are the property of their respective owners. The specification and the column type are subject to change without notice due to continual improvements.