2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Department of Haematology & lood Transfusion East Kent Hospitals University NHS Foundation Trust William Harvey Hospital Kennington Road Willesborough Ashford TN24 0LZ Contact: Dr Gillian Evans Tel: +44 (0) 1233 633 331 Fax: +44 (0) 1233 643 331 E-Mail: gillian.evans4@nhs.net Website: www.ekhuft.nhs.uk Laboratory locations: below s covered by the organisation and their relevant activities details Activity code Address William Harvey Hospital Kennington Road Willesborough Ashford TN24 0LZ Haematology lood Transfusion (including the issue of red cells and blood products) A Address Kent & Canterbury Hospital Ethelbert Road Canterbury CT1 3NG Haematology lood Transfusion (including the issue of red cells and blood products) lood Fridge at rabourne Ward Address Queen Elizabeth the Queen Mother Hospital St Peters Road Margate CT9 4AN Haematology lood Transfusion (including the issue of red cells and blood products) C Assessment Manager: HL 1 of 8
Site activities performed away from the locations listed above: details Activity uckland Hospital Coombe Valley Road Dover CT17 0HD Royal Victoria Hospital Radnor Park Avenue Folkestone CT19 5N eneden Hospital Goddards Green Road Cranbrook TN17 4AX lood fridge storage only Fridge managed by Laboratory One Ashford Healthcare Ltd Kennington Road Ashford TN24 0YS lood fridge storage only Fridge managed by Laboratory rabourne Ward Kent & Canterbury Hospital Cantebury Kent lood fridge storage only Fridge managed by Laboratory Kent Surrey & Sussex air ambulance trust (HEMS) Provision of O negative RC in Golden hour boxes Assessment Manager: HL Page 2 of 8
DETAIL OF ACCREDITATION HUMAN ODY FLUIDS Haematological examination activities for the purpose of clinical diagnosis Full lood Count (FC) Including:- Haemoglobin White lood Count Platelet Count Red lood Count Haematocrit MCV MCH MCHC RDW Neutrophil count Lymphocyte count Monocyte count Eosinophil count asophil count Reticulocyte Count NRC Count Sysmex XE2100 Flow Cell/various with specific reference to SOP: HAE-LP-110a HAE-LP-100a-c & e-f lood Film for detection of abnormalities Manual blood films using Light microscopy HAE-LP-119/123 Automated blood film stainers: Hematek 2000 HAE-LP-102 using May Grundwald and Giemsa stain Shandon Varistain HAE-LP-142 using May Grundwald and Giemsa stain C lood Film for detection of abnormalities Sysmex Sp1000i HAE-LP-142/102/201 HAE-LP-202/HAE-LP-203 A Assessment Manager: HL Page 3 of 8
HUMAN ODY FLUIDS Haematological examination activities for the purpose of clinical diagnosis Venous Whole lood Erythrocyte sedimentation rate (ESR) Starsed InteRRLiner / Autocompact Westergren - 1Hr HAE-LP-160/206/207/307/308 A,C Venous Whole lood Detection of infectious mononucleosis heterophile antibodies iokit Monogen commercial kit, latex micro particle immunoassay HAE-LP-135 lood Parasitology Malaria Parasite speciation (P falciparum, P. vivax, P ovale, P malariae) Manual staining thin films with Giemsa HAE-LP-119/122/123 Haemoglobinopathy: Quantitation of HbA2 Quantitation of HbF Quantitation of HbS Identification/Quantitation of other Variant Hb Sebia Capillary 2 HPLC with reference to: HAE-LP-130 HAE-LP-213 C Glucose-6-Phosphate Dehydrogenase Deficiency Screen Trinity iotech Fluorescence With reference to: HAE-LP-301 A Sickle cell screen Manual sickle solubility Sickledex kit HAE-LP-130 Venous blood: Citrated D Dimer STAGO STA-R Evolution, Turbidimetric HPA-LP-154 A,C Assessment Manager: HL Page 4 of 8
HUMAN ODY FLUIDS Haematological examination activities for the purpose of clinical diagnosis Venous blood: Citrated D Dimer STAGO STA-R MAX HPA-LP-208 Venous lood: Citrated Clotting Screen Prothrombin time/inr APPT/APPTR Fibrinogen (Clauss) Thrombin Time Reptilase PT-50:50 Mixing Studies APTT 50:50 Mixing Studies STAGO STA-R Evolution, Viscosity /Chromogenic HPA-LP-152/151 HPA-LP-152/154 HPA-LP-022/152/155 HPA-LP-022/152/156 HPA-LP-022 HPA-LP-023 STAGO STA-R MAX HPA-LP-201 HPA-LP-203 HPA-LP-205 HPA-LP-206 A, C Flow Cytometry for Immune Monitoring Venous Whole lood Detection of Human Leukocyte Antigen (HLA) 27 eckman Coulter Navios - Flow Cytometry HAE-LP-205p/205q/302 Venous Whole lood Lymphocyte Subsets CD-4 CD-8 CD-3 eckman Coulter Navios & Aquios- Flow Cytometry HAE-LP- 205p/205q/205r/205s/205v/212f/21 2g/310 Assessment Manager: HL Page 5 of 8
HUMAN ODY FLUIDS lood Transfusion examination activities for the purpose of clinical diagnosis: Venous blood: red cells lood Grouping ORhD positive ORhD negative ARhD positive A RhD negative RhD positive RhD negative A RhD positive A RhD negative IH1000 and Gel Station, LT-LP-073/074a,b, LT-LP-009/111/113 Antigen phenotyping C, c, E, e, K, Fya, Fyb, Jka, Jkb, M, N, S, s, Lea, Leb, P1, Kpa, Lua and Cw IH1000 and Gel Station, LT-LP-073/074a,b, LT-LP-009/111/113 Venous blood: plasma, red cells Antibody Screen following systems: Rh, C,D,E,c,e, C w Kell K, k, Kp a Duffy Fy a, Fy b MNSs M, N, S, s Kidd JK a, Jk b Lutheran Lu a Lewis Le a, Le b P P1 A1 IH1000 and Gel Station, LT-LP-073/074a,b, LT-LP-009/111/113 Venous blood: plasma, red cells Compatibility Testing: Serological Crossmatch - serological compatibility testing between donor red cell antigens and patient plasma. IH1000 and Gel Station, LT-LP-073/074a,b, LT-LP-009/111/113 Assessment Manager: HL Page 6 of 8
HUMAN ODY FLUIDS lood Transfusion examination activities for the purpose of clinical diagnosis : Venous blood: plasma, red cells lood Grouping: ORhD positive ORhD negative ARhD positive A RhD negative RhD positive RhD negative A RhD positive A RhD negative Manual method using Diamed Card/ incubator & centrifuge - LT-LP-074c Venous blood: plasma Antibody Screen Rh, C,D,E,c,e, C w Kell K, k, Kp a Duffy Fy a, Fy b MNSs M, N, S, s Kidd JK a, Jk b Lutheran Lu a Lewis Le a, Le b P P1 A1 Manual method using Diamed Card/ incubator & centrifuge - LT-LP-074c Venous blood: plasma Antibody Identification (IAT) Rh, C,D,E,c,e, C w Kell K, K, Kp a Duffy Fy a, Fy b MNSs M, N, S, s Kidd JK a, Jk b Lutheran Lu a Lewis Le a, Le b P P1 A1 Manual method using Diamed Card/ incubator & centrifuge - LT-LP-005/041/043 Assessment Manager: HL Page 7 of 8
HUMAN ODY FLUIDS lood Transfusion examination activities for the purpose of clinical diagnosis : Venous blood: plasma Antibody Identification (Enzyme) Rh, C,D,E,c,e, C w Kell K, K, Kpa Kidd JK a, Jk b Lutheran Lu a Lewis Le a, Le b P P1 A1 Manual Diamed Card/ incubator & centrifuge - LT-LP-005/042/043 Venous blood: plasma Direct Antiglobulin Test Manual Column agglutination technique Diamed Card/ incubator & centrifuge LT-LP-044/045 Venous blood red cells Antigen phenotyping C, c, E, e,, Fya, Fyb, Jka, Jkb, M, N, S, s, Lea, Leb, P1, Kpa, Lua, Cw and K Manual Column agglutination technique Diamed Card/ incubator & centrifuge LT-LP-028 Venous blood red cells Compatibility testing Manual Column agglutination technique Diamed Card/ incubator & centrifuge LT-LP-011/013/012/014/056/061 Venous Whole lood Detection and Estimation of Foetal Maternal Haemorrhage Manual Kleihauer slide acid elution technique/microscopy procedures using Shepherds stain and Guest Medical Foetal Cell Detection Kit with reference to; LT-LP-119 A,C END Assessment Manager: HL Page 8 of 8