Protocol #: 12-016 Final https://www.ir4org/about-environmental-horticulture/ehc-registration-support-research/envhort-researcher-resources/#protocols p 1 of 10 Objective: Determine efficacy of new active ingredient formulations and new biopesticides for managing root, crown and stem rots of ornamental plants caused by Fusarium species. Experimental Design: Plot Size: Must be adequate to reflect actual use conditions. Replicates: Minimum of 4. Application Instructions: For 2012, soil or plant material is to be inoculated with Fusarium species prior to fungicide treatment. A number of different application methods can be employed based on the disease system studied. Application rates and intervals follow in tables below for foliar, drench, corm/root dip, top dress, and soil incorporation application methods. Multiple application types may be utilized in the same experiment, but rates must be used consistent with the different methods.. Applications should be made using application equipment consistent with conventional commercial equipment. Calibrate application equipment prior to application. Read available labels before making applications. Avoid treating plants under unusually extreme environmental conditions. Target Species: TBD. Contact your regional coordinator for specifics. Plant Hosts: Use a plant host suitable for target species, recording species and variety used. Use Site: May be greenhouse, field container or field in-ground. Please specify in final report. Evaluations: Record disease severity and incidence 0, 14, and 28 days after application for herbaceous plant material. For woody plant material, record disease severity and incidence 0, 14, 28, 42 and 56 days after initial application. Record plant height & width at initial and final evaluations only. Record phytotoxicity at each rating date on a scale of 0 to 10 (0 = no phytotoxicity; 10 = complete kill). If phytotoxicity is observed in treated plants, take pictures comparing treated and untreated plant material. Recordkeeping: All operations, data and observations appropriate to this study should be recorded. It is helpful to review the Ornamental Horticulture Research Report Form in advance. Keep detailed records of weather conditions including temperature, precipitation and/or irrigation, and relative humidity with a minimum of high, low and average daily temperatures. Other information to record includes soil-type or soil-less media, application equipment, irrigation (type & frequency), liner size, plant height & width, and plant growth stage at application and data collection dates. Content of reports should be sufficient to fully understand how the experiment was conducted. Photographs often illustrate experimental design, site conditions, and impacts of treatments very well. It is encouraged to include a picture or two of the greenhouse, field or landscape where the experiment is sited. It is highly encouraged that pictures illustrating treatment effects are taken if and when these impacts are visually apparent. If different application methods or evaluations are made, please clearly specify differences in final report and explain reason for change. Treatments: See table on the following page.
p 2 of 10 Foliar Treatments: Foliar Rates A 1 Acibenzolar 0.5 oz 2 0.75 oz 7 d 3 RootShield Plus BW240 WP (Trichoderma harzianum T-22 + 8 oz Trichoderma virens G-41) 4 Cg100 38.4 fl oz 5 Palladium (cyprodinil and fludioxinil) 6 fl oz 7 14 d 6 SP2169 12.3 fl oz 7 Tourney (metconazole) 2 oz 8 4 oz 9 Trinity (triticonazole) 8 fl oz 10 12 fl oz Standards 11 Standard Control See below See below 12 Untreated Uninoculated -- -- 13 Untreated Inoculated -- -- Standards a 3336 (thiophanate-methyl) 16 oz b Medallion (fludioxonil) 4 oz 7 14 d c Terraguard (triflumizole) Special Application Instructions (Foliar) Must be applied twice before exposing to pathogen or inoculation Protocol #: 12-016 Final B Compass O (trifloxystrobin) DisArm Heritage (azoxystrobin) Insignia (pyraclostrobin) Pageant 38WG Torque (tebuconazole) 0.5 oz + 1.8 oz 0.75 oz + 2.7 oz 4 fl oz 1.8 oz 12 oz 14 day 10 fl oz Apply with sufficient coverage for foliar disease protection prior to first sign of infection.
p 3 of 10 Directed Spray Treatments (to crown or base of plant): Directed Spray Rates A 1 Acibenzolar 0.5 oz 2 0.75 oz 3 RootShield Plus BW240 WP 8 oz (Trichoderma harzianum T-22 + Trichoderma virens G-41) 4 Cg100 38.4 fl oz 5 Palladium (cyprodinil and 6 fl oz fludioxinil) 7 d 7 14 d 6 SP2169 12.3 fl oz 7 Tourney (metconazole) 2 oz 8 4 oz 9 Trinity (triticonazole) 8 fl oz 10 12 fl oz Standards 11 Standard Control See below See below 12 Untreated Uninoculated -- -- 13 Untreated Inoculated -- -- Standards a 3336 (thiophanate-methyl) 16 oz b Medallion (fludioxonil) 4 oz c Terraguard (triflumizole) 7 14 d Special Application Instructions (Directed Spray) Must be applied twice before exposing to pathogen or inoculation Protocol #: 12-016 Final B Compass O (trifloxystrobin) DisArm 0.5 oz + 1.8 oz 0.75 oz + 2.7 oz 4 fl oz 7 14 d Flagship 25WG 6 oz 7 14 d Flagship 25WG + Heritage 6 oz + 4 oz 7 14 d
p 4 of 10 Directed Spray Rates Heritage (azoxystrobin) 1.8 oz 7 14 d Special Application Instructions (Directed Spray) Insignia (pyraclostrobin) Pageant 38WG 12 oz 14 day Apply with sufficient coverage for foliar disease protection prior to first sign of infection. Torque (tebuconazole) 10 fl oz Protocol #: 12-016 Final
p 5 of 10 Drench Application Treatments: Drench Rates Special Application Instructions (Soil Drench) A 1 Acibenzolar 0.125 oz 21 days Apply as a drench 2 weeks prior to 2 0.25 oz inoculations 3 RootShield Plus BW240 WP (Trichoderma harzianum T-22 + Trichoderma virens G-41) 6 oz ½ rate at 10 to 12 weeks Apply material 72 hours before pathogen inoculation or dip plants before planting into inoculated soil 4 Cg100 38.4 oz 5 Palladium (cyprodinil and 6 oz fludioxinil) 21 d 6 SP2169 12.3 oz per 100 gal Optional: 24.6 oz per 100 gal 7 Tourney (metconazole) 2 oz Apply as a drench 8 4 oz 9 Trinity (triticonazole) 8 fl oz 10 12 fl oz Apply as a drench Standards 11 Standard Control See below See below See below 12 Untreated Uninoculated -- -- -- 13 Untreated Inoculated -- -- -- Standards a 3336 (thiophanate-methyl) b Medallion (fludioxonil) 2 oz 21 days Apply as a drench to thoroughly wet ¾ of the root zone c Terraguard (triflumizole) Protocol #: 12-016 Fianl B Compass O (trifloxystrobin) DisArm 0.125 oz + 0.45 oz 0.25 oz + 0.9 oz 4 oz 1-2 application 28 day 2 application 28 day The determination of application frequency can be determined by the researcher based on their I realize this will vary. Apply at 1-2pts/sqft The determination of application frequency can be determined by the researcher based on their
p 6 of 10 Flagship 25WG Flagship 25WG + Heritage Flagship 25 WG + Hurricane Heritage (azoxystrobin) Drench Rates 6 oz 6 oz + 0.9 oz 6 oz + 1.5 oz 2 applications 28 day 2 applications 28 day Special Application Instructions (Soil Drench) The application frequency can be determined by the researcher based on their Apply at 1-2 pts per sqft. 1 application 28 day 0.9 oz 28 days Drench to thoroughly wet ¾ of the root zone Hurricane (fludioxonil + mefonaxam) 1.5 oz Insignia (pyraclostrobin) 6.1 fl oz Pageant 38WG 12 oz 14 day Apply with sufficient coverage for foliar disease protection prior to first sign of infection. Torque (tebuconazole) 10 fl oz Protocol #: 12-016 Fianl
p 7 of 10 Corm Dip Treatments: Corm Dip Rates A 1 Acibenzolar 0.125 oz none 2 0.25 oz 3 RootShield Plus BW240 WP 6 oz ½ rate at 10 to (Trichoderma harzianum T-22 + 12 weeks Trichoderma virens G-41) 4 Cg100 38.4 oz 5 Palladium (cyprodinil and 6 oz Special Application Instructions (Corm Dip) Apply material 72 hours before pathogen inoculation or dip plants before planting into inoculated soil fludioxinil) 6 SP2169 12.3 oz per 100 gal Optional: 24.6 oz per 100 gal 7 Tourney (metconazole) 2 oz 8 4 oz Standards 9 Standard Control See below See below See below 10 Untreated Uninoculated -- -- -- 11 Untreated Inoculated -- -- -- Standards a 3336 (thiophanate-methyl) b Medallion (fludioxonil) 2 oz none c Terraguard (triflumizole) Protocol #: 12-016 Fianl B Compass O (trifloxystrobin) DisArm Heritage (azoxystrobin) Torque (tebuconazole) 0.125 oz + 0.45 oz 0.25 oz + 0.9 oz 0.5 oz + 1.8 oz 4 oz 4.0 oz 0.9 oz 1.8 oz 10 fl oz none none
p 8 of 10 Top Dress Treatments (Application to soil surface either spray or granular broadcast, followed by irrigation): A 1 Acibenzolar 2 3 RootShield Plus BW240 WP (Trichoderma harzianum T-22 + Trichoderma virens G-41) Top Dress Rates (per 1000 sq ft) (0.08 g) 4 Cg100 26.0 ml 5 Palladium (cyprodinil and 1oz fludioxinil) (0.16 g) 32 oz per acre ½ rate at 10 to 12 weeks Container Rate (5oz ) 6 SP2169 8.35 ml Special Application Instructions (Soil Drench) 21 days Topdress rate given in parenthesis is based on a drench rate of 0.125 and 0.25 with a 1 pt/sqft application volume. Apply material 72 hours before pathogen inoculation or dip plants before planting into inoculated soil Topdress rate given in (parenthesis) is based on a drench rate of 4oz /100gal with a 1 pt/sqft application volume. This rate should be used if the trial is for container grown plants, otherwise use other rate. Protocol #: 12-016 Fianl 7 Tourney (metconazole) 2.8 g 8 5.6 g 9 Trinity (triticonazole) 5.43 ml 10 8.14 ml Standards 11 Standard Control See below See below 12 Untreated Uninoculated -- -- 13 Untreated Inoculated -- -- Standards a 3336 (thiophanate-methyl) 10.8 ml b Medallion (fludioxonil) 0.75 oz per 1000 sq ft c Terraguard (triflumizole) Container Rate (2.5oz) 21 days This top rate is based on label use limit for outdoor production assuming 2 applications allowed per year. The rate given in (parenthesis) is based on a drench rate of 2oz /100gal with a 1 pt/sqft application volume. This rate should be used if the trial is for container grown plants, otherwise use other rate.
p 9 of 10 B Compass O (trifloxystrobin) DisArm Flagship 25WG Flagship 25WG + Heritage Flagship 0.22G Top Dress Rates (per 1000 sq ft) (0.08g + 1oz) 2.71 ml 2.71 ml 0.55 oz/1000 linear ft (3 ft row spacing) 0.4 oz + 0.4 oz Container Rate (7.5oz + 1oz) 44 oz (2.76 lb) per 1000 ft Special Application Instructions (Soil Drench) Topdress rate given in parenthesis is based on a drench rate of 0.125 and 0.9oz/100 gal with a 1 pt/sqft application volume. This top rate is based on label use limit for outdoor production assuming 2 applications allowed per year. The rate given in (parenthesis) is based on a drench rate of 6oz +0.9oz /100gal with a 1 pt/sqft application volume. This rate should be used if the trial is for container grown plants, otherwise use other rate.. Flagship 0.22G + Heritage 44 oz (2.76 lb) + 0.4 oz per 1000 ft Field application rate Protocol #: 12-016 Fianl Heritage (azoxystrobin) Insignia (pyraclostrobin) Pageant 38WG Torque (tebuconazole) 0.4 oz Container rate (1 oz) 10 ml per 100 gal delivering 0.24 ml product per 11.8 sq ft. 14 day This top rate is based on label use for outdoor production as a banded treatment. The rate given in (parenthesis) is based on a drench rate of 0.9oz /100gal with a 1 pt/sqft application volume. This rate should be used if the trial is for container grown plants, otherwise use other rate.
Protocol #: 12-016 Final p 10 of 10 Sources for Products: Product Contact Phone Email 3336 Cleary, Rick Fletcher 800-524-1662 rick.fletcher@clearychemical.com Acibenzolar Syngenta, Nancy Rechcigl 941-238-7413 nancy.rechcigl@syngenta.com Cg100 AMVAC, Ned French 949-260-1212 NedF@amvac-chemical.com DisArm OHP, Jeff Dobbs 770-992-0121 jdobbs@ohp.com Compass 0 OHP, Jeff Dobbs 770-992-0121 jdobbs@ohp.com Flagship Syngenta, Nancy Rechcigl 941-238-7413 nancy.rechcigl@syngenta.com Heritage Syngenta, Nancy Rechcigl 941-238-7413 nancy.rechcigl@syngenta.com Hurricane Syngenta, Nancy Rechcigl 941-238-7413 nancy.rechcigl@syngenta.com Insignia BASF, Kathie Kalmowitz 919-270-4592 kathie.kalmowitz@basf.com Medallion Syngenta, Nancy Rechcigl 941-238-7413 nancy.rechcigl@syngenta.com Pageant BASF, Kathie Kalmowitz 919-270-4592 kathie.kalmowitz@basf.com Palladium Syngenta, Nancy Rechcigl 941-238-7413 nancy.rechcigl@syngenta.com RootShield Plus BioWorks, Matt Krause 800-877-9443 x 335 mkrause@bioworksinc.com SP2169 SePRO, Tyler Koschnick 317-216-8280 tylerk@sepro.com Torque Cleary, Rick Fletcher 800-524-1662 rick.fletcher@clearychemical.com Tourney Valent, Joe Chamberlin 770-985-0303 jcham@valent.com Trinity BASF, Kathie Kalmowitz 919-270-4592 kathie.kalmowitz@basf.com Reports: Reports are due 60 days after last data collection date. Submit reports electronically on the standard IR-4 Ornamental Horticulture Research Report Form. The basic report can be sent in MS Word, the recordkeeping information as pdf or other electronic documents, the raw data in MS Excel or other suitable program such as ARM, and photographs can be submitted as picture embedded in the report or as separate jpg, bmp, or tiff files. Please direct questions to: Cristi Palmer, IR-4 HQ, Rutgers University, 500 College Road East, Suite 201W, Princeton, NJ 08540, Phone 732-932-9575 x4629, palmer@aesop.rutgers.edu OR Ely Vea, 308 Aston Forest Lane, Crownsville, MD 21032, Phone & FAX#: 410-923-4880, E-mail: evvea@comcast.net. Draft Date: 8/16/2012 Revised By: CLP