T.E.N. for a High Producing Holstein Herd. Rodrigo Garcia, DVM PhD Technical Service Intn l Ruminants - TechMix Global Czech Republic, Feb 2015

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T.E.N. for a High Producing Holstein Herd Rodrigo Garcia, DVM PhD Technical Service Intn l Ruminants - TechMix Global Czech Republic, Feb 2015

Dairy Events Birth Pre & Post Weaning Transportation Dehydration Off-Feed periods Temperature changes Calving Feed changes Timed Event Nutrition The right nutritional assistance at the right time.

NELSON FAMILY COMPANIES Currently employing 285 employees

Production Facility Stewart, MN Opened, January, 2007

Fresh Cow Program Current Dairy Products Heat Stress Program Rehydrate. Recover. Perform. Rumen Efficiency Program..now A Calf Program

Soluble Powder

Midwest USA, 12 Herd Summary trial Why use Fresh Cow YMCP? Reduced metabolic problems associated with calving YMCP provides KEY nutrients at a very critical moment *Mike Hutjens, University of Illinois Improved milk production Cows helps cows recover faster, with better rumen function & appetite

20 L 1 L Drinkable post calving nutrition supplement (calcium, energy, electrolytes, vitamins and live yeast) Also applied by Drench (recommended) Dairy cow Right after calving 1 DOSE (500 G), 1 COW, 1 TIME Prevent: Ketosis, Milk Fever, Abomasal Displacement, Retained placenta Promote: Ruminal Activation, Rehydration Improve: Milk production (1-2 Kg/day) and quality (fat & protein)

Proof of Performance YMCP Calcium Trial -Summary Results- Spain, August 2014 Objectives: Determine the efficacy of Fresh Cow YMCP to stimulate blood calcium levels in fresh cows To compare the performance of YMCP Powder and YMCP Paste in stimulating blood calcium levels

Location, facilities & protocol: Farm: Mas Janer in Girona, Spain Farmer: Marc Xifra 220 milking cows TechMix knows this farm from previous visits by Toni Ferro and Jon Gelschus. It is a considered a medium-high performance herd (ME 305 ave was 24 200 lbs (10 977Kg) for cows in trial) with minimum health issues The trial was supervised by a local veterinarian: Antoni Margenat (Veterinaris Vilubí) Blood samples were collected, processed, and later sent to the University of Barcelona: Biochemical Department of Veterinary College for calcium analysis

Blodd Calcium - mg/dl Blood Calcium vs Time - YMCP trial, Spain 2014 12 11 10 Calving YMCP Control 9 8 7 6 5 4 3 2 <58h <12 <6 0-3 >3 >6 >9 >12 >16 >24 >36 Time - hours before/after calving

Soluble Powder Application: -> Drinking by Pail Mix 500 grams in 20 liters of warm water KEY POINT: Cows will only drink YMCP within 30 minutes after calving

Soluble Powder Application: By Drench Pump Mix 500 grams with 20 liters of warm water Manual pump Drench mate system for large dairies

Soluble Powder Application: -> Bottle feed Mix 500 grams in 2 liters of water

Soluble Powder Application: By drench pistol/syringe Mix 500 grams with 600 mls of water to create paste Apply via drench syringe or pistol

Maximum Convenience Paste 375 gram tube Ready to Use 1 tube per cow (dosage rate) Requires applicator pistol

Soluble Powder Available in 500 gram powder paks To be mixed with water Drink from pail Via stomach pump Make paste Ready to use Maximum convenience

20 grams bolus Any moment when the cow is either off fed, depressed or stressed. DAIRY COW 1 BOLUS EVERY 12 HOURS, MAX 3 BOLUSES Ruminal activation that leads to an appetite improvement and overcoming of individual stress periods.

Pre and/or post Calving (1 bolus every 12 hours, max 3 boluses) When the cow changes group or pen (1 bolus every 12 hours, max 3 boluses) Off feed animals (1 bolus every 12 hours, max 3 boluses) Following antibiotic treatment (1 bolus every 12 hours, max 3 boluses) Following foot trimming (1 bolus every 12 hours, max 3 boluses) Following any kind of treatment, intervention or cow manipulation (Shows, vaccination, transport) (1 bolus every 12 hours, max 3 boluses): - Abomasal displacement - Retained Placenta - Ketosis Acidosis

VITAMINS AND PROVITAMINS Vitamin E + 6 grams of Niacin MICRO-ORGANISMS Saccharomyces cerevisiae 4.6 x 10 11 cfu/kg DIGESTIBILITY ENHANCER Fermentation product of Aspergillus oryzae 250g/kg Improve: Ruminal activation Increase appetite Overcoming of individual stressful periods. Control Rumen Yeast P - Value Average ph 5.49 6.05 <0.01 Below ph 5.6 - % of time 68.3 25.1 <0.01 Below ph 6.0 - % of time 90.6 46.7 <0.01

New data from clients in Turkey 2 large dairy operations in Turkey (July/August 14) Measuring impact of TriStart caps in problem fresh cows Measuring impact of TriStart caps for off feed events during lactation

SCR Rumination Technology Real time measurement of cows rumination time - Allows dairy manager to detect & track sub-optimal rumen function, and take steps to correct this

The need to ruminate A normal cow will ruminate approximately 1/3 of the day = 8-9 hours/day High producing dairy cows will eat more and tend to ruminate longer Functions: - Break down fibrous material and help the passage through the rumen - Saliva production = rumen buffering

Problem Fresh Cows Rumination (minutes per day) 600 500 400 Healthy cow 300 200 100 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Rumination 161 204 330 388 461 448 481 407 423 475 450 468 521 482 Healthy cow line 300 350 380 410 430 450 470 483 490 500 500 500 500 500 A healthy cow will ideally return to her target rumination (450-500 minutes/day) within 6-10 days (green dotted line). The BLUE line is an off feed cow whom was given 1 x TriStart capsule/day for 3 days

5.VIII 6.VIII 7.VIII 8.VIII 9.VIII 10.VIII 11.VIII 12.VIII 13.VIII 14.VIII 15.VIII 16.VIII 17.VIII 18.VIII 19.VIII 20.VIII 21.VIII 22.VIII 23.VIII Off Feed events 800 Rumination (minutes per day) 700 600 500 400 Healthy cow 300 200 100 0 Mid lactation cow suffering from off feed event. After 3 days of declining rumination, dairy manager applied 1 TriStart capsule. Within 24 hours the cow recovered feed intake and the improved rumination activity.

Consumption of sugars : competition with lactate producing bacteria Oxygen scavenging Decreased rumen lactic acid : stimulation of lactate consuming bacteria Stabilization of ruminal function (bacteria, protozoa, fungi) Raizes and stabilizes ph Limits risk of acidosis Increases fibre degradation IMPROVE MILK YIELD BEST ENERGY BALANCE IMPROVE REPRODUCTIVE EFFICIENCY

VITAMINS AND PROVITAMINS Vitamin E + Niacin MICRO-ORGANISMS Saccharomyces cerevisiae 1.0x10 12 cfu/kg DIGESTIBILITY ENHANCER DAIRY COW 10-20 g/cow/day Fermentation product of Aspergillus oryzae 250g/kg Transition cow 14-21 days Precalving and 14-21 days Postcalving (adaptable to farm conditions) To prepare calving, for a good milking starting and preparing next reproduction time (Better signs of estrus). More milk and quality Feed changes, weather changes, winter diarrheas 7 10 days Promoting rumen bacteria growth Reducing acidosis Improve digestion when mycotoxins are present.

IRTA: Loose housing Control 5.51 Live Yeast 6.06 SARA zone SARA zone Minnesota Uty: Tie-stall cows Ave ph 5.95 Ave ph 6.53

Reproductive efficiency Jack Britt 1992, development of the follicle more than 80 days before insemination North Florida Holsteins 2003 TriMic in lactation Conservation of body condition Fertility rate at first service improved from 37.8% to 45.4% with TriMic.

Electrolytes Betaine Energy Vitamins Soluble powder product for drinking water (energy, electrolytes, betain and vitamins) 1 kg/500l water Niacin DAIRY COW 60 gr/cow/day By water or mixed with the TMR Transition / Fresh Cows High production Cows Heat Stress Sick Pen Diarrhea

Soluble Powder Product for drinking Water: 1 kg/500l water CALVES 15-30-60 gr/calf/day via water or with the feedstuffs (depending on BW) Receiving cattle Pre-Harvest Adaptation Management (dehorning ) Diarrhea

DAIRY COWS Heat & Cold Stress 130-150 g/cow/day (7 cows/kg product/day) Transition or Fresh Cows and High production cows 115 170 g/cow/day (6-9 cows/kg product/day) Depending on the production level Sick Pens 115 g/cow/day- (8-9 cows/kg product/day) Weather changes, Winter Diarrhea 115 g/cow/day- (8-9 cows/kg product/day)

CALVES, HEIFERS AND PREHARVEST 70-150 g in all stages when the calves suffer heat stress and dehydration Before and After transport :100 150 gr for rehydration Mixed with feedstuff: 2 3 days before and 2 3 days after. ***Pre Harvest: 2 days before the transport, 500 g first day and 500 g the second day. The transport should be the third day. Fed at 30% in a concentrate 2 days before slaughter. Benefits: Higher weight and better Meat Quality (less dark cutters)

Improved water and feed intake and general rehydration in high production or sickness periods and in Heat Stress. Increased reserves of glycogen in liver and muscle and their availability for synthesis of lactose = milk production. Improved water and feed intake = Higher milk yield Regarding MEAT QUALITY, BBL increases energy reserves of glycogen in muscle and their availability for production of lactic acid. Lactic acid lowers ph of meat and improves its quality avoiding Dark Cutters.

Fresh Cow Program Current Dairy Products Heat Stress Program Rehydrate. Recover. Perform. Rumen Efficiency Program..now A Calf Program

Thank You Timed Event Nutrition for a high producing Holstein Herd Any Question? Rodrigo Garcia, DVM PhD rodrigogarcia@techmixglobal.co m