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Sepax Technologies, Inc. Sepax Technologies, Inc. develops and manufactures products in the area of chemical and biological separations, biosurfaces and proteomics. Sepax product portfolio includes ) liquid chromatography columns and media, ) SPE and Flash chromatography columns and tubes, ) bulk resin for preparative separation and process chromatography, and ) natural product and Chinese traditional medicine separation and purification. Super Critical Fluid Chromatography Sepax SFC-Pyridine... Sepax SFC-SCX... Sepax SFC-Diol... Sepax SFC-Cyano... Sepax SFC-Amino... 6 Sepax SFC-Silica... 7 Column price list... 8- www.sepax-tech.com -877-SEPAX-US

AU Sepax SFC-Pyridine SFC test chromatogram Si Si CH CH N Vendor X N(#)=, Silica: Spherical, high purity (< ppm metals) Pore size: Å Particle size:.8,.,,, 7, and m Pore volume:. ml/g Surface area: m /g Phase structure: Polymeric and no endcapping % Carbon: 8.% Coverage: ~. mol/m.7.6...... 6 8 6 8 Column: SFC-Pyridine (µm, Å,.6xmm) Mobile phase: 8% Hexane/% Ethanol Flow rate:. ml/min Detector (UV): nm Injection volume:. µl Temperature: Ambient ( o C) Sample:. Pyridine. Aniline. Phenol. Uracil. p-aminobenzoic acid 6 Vendor Y N(#)=8,86 Sepax N(#)=8, 7 8 Column: Pyridine phases (µ,.6x mm) Mobile phase: C /MeH Gradient: t (min) 6 7 8 9 MeH(%) Flow rate:. ml/min Detection (DAD): nm Instrument: Berger Analytical SFC Sample:. Dimethylphthalate. Caffeine. Uracil. Cortisone Well controlled chemistry of polymeric monolayer High column-to-column reproducibility Recommended for separations of acidic, neutral and basic organic compounds, as well as the pharmaceuticals Sepax SFC-Pyridine Products SFC-Pyridine Pore size P/N m,.6xmm Å 69-6 m,.6xmm Å 69-6 m,.6xmm Å 69-6 m,.xmm Å 69- m,.xmm Å 69- m,.xmm Å 69- m, xmm Å 69- m, xmm Å 69- m, xmm Å 69-9 www.sepax-tech.com -877-SEPAX-US

Sepax SFC-SCX Phase Structure Si Si Si Technical Specifications Silica: Spherical, high purity (< ppm metals) Pore size: Å Particle size:.8,.,,, m Pore volume:. ml/g Surface area: m /g Phase structure: Polymeric and mixed mode % Carbon:.% Coverage:. mol/m Description Sepax SFC-SCX is a silica based mixed-mode strong cation exchange phase. Its phase has a mixed chemical structure of sulfonic acid and phenyl group. This mixed mode of strong cation-exchange and hydrophobicity offers high selectivity and high resolution separation for cationic/basic and nitrogen containing compounds as well as desirable retention for a variety of weak cationic and neutral organic compounds. A uniform, polymeric bonding of Sepax SFC- SCX enhances its stability under various separation conditions. Sepax SFC-SCX has expanded its applications from the traditional SCX separation of cationic and nitrogen containing compounds to weak cationic and neutral organic molecules. Examples include separation of analytes of amines and polyamines, such as alkaloids, peptides, codeine, cough and cold ingredients. S H Highly controlled chemistry of polymeric monolayer formation and end-capping Mixed chemical structure of sulfonic acid and phenyl group Mixed-mode of ion-exchange and hydrophobic interaction enabling high selectivity and appropriate retention for a variety of compounds Polymeric bonding and end-capping to achieve the exceptionally high stability ph stability:.-8. Suitable for separations of a complex of cationic, nitrogen containing, and neutral compounds mau 8 6 Column: Sepax SFC-SCX (.6x mm, m) Mobile phase: mm phosphate buffer, ph. Flow rate:. ml/min (8 bar) Detector: UV 8 nm Injection volume: L Temperature: Ambient ( o C) Compounds:. Uridine (. mg/ml). Cytidine (. mg/ml). Guanosine (. mg/ml). Inosine and impurities (. mg/ml). Adenosine (. mg/ml) 6 8 min Sepax SFC-SCX Products Sepax SFC-SCX Pore size P/N m,.6xmm Å 66-6 m,.6xmm Å 66-6 m,.6xmm Å 66-6 m,.xmm Å 66- m,.xmm Å 66- m,.xmm Å 66- m, xmm Å 66- m, xmm Å 66- m, xmm Å 66- www.sepax-tech.com -877-SEPAX-US

AU mau Sepax SFC-Diol SFC test chromatogram 7 Si Si CH CH CH H CH CHCH H 6 nm (N=6,) Silica: Spherical, high purity (< ppm metals) Pore size: Å Particle size:.8,.,,, m Pore volume:. ml/g Surface area: m /g Phase structure: Monomeric and fully endcapped % Carbon: 8.8% Coverage: ~. mol/m.8.7.6. nm 6 7 Column: Sepax SFC-Diol (µ,.6x mm) Mobile phase: C /MeH Gradient: t (min) 6 7 8 9 MeH(%) Flow rate:. ml/min Detection (DAD): nm Instrument: Berger Analytical SFC Sample:. Dimethylphthalate. Caffeine. Uracil. Cortisone 8 9..... 6 7 8 9 Well controlled chemistry of polymeric monolayer High column-to-column reproducibility Polar phase for separations of acidic, neutral and basic organic compounds, as well as the pharmaceuticals High selectivity and efficiency for separations Sepax SFC-Diol Products Column: SFC-Diol (µm, Å,.6xmm) Mobile phase: 8% Hexane/% Ethanol Flow rate:. ml/min Detector (UV): nm Injection volume:. µl Temperature: Ambient ( o C) Sample:. Phenol. -Nitrophenol.,-Biphenol Sepax SFC-Diol Pore size P/N m,.6xmm Å 66-6 m,.6xmm Å 66-6 m,.6xmm Å 66-6 m,.xmm Å 66- m,.xmm Å 66- m,.xmm Å 66- m, xmm Å 66- m, xmm Å 66- m, xmm Å 66- www.sepax-tech.com -877-SEPAX-US

AU mau Sepax SFC-Cyano SFC test chromatogram Si CH Si (CH ) CN 7 6 CH Silica: Spherical, high purity (< ppm metals) Pore size: Å Particle size:.8,.,,, m Pore volume:. ml/g Surface area: m /g Phase structure: Monomeric and fully endcapped % Carbon: 7.% Coverage: ~. mol/m.6. (N=,6) Sym=.7 (N=,) 6 7 8 Column: Sepax SFC-Cyano (µ,.6x mm) Mobile phase: C /MeH Gradient: t (min) 6 7 8 9 MeH(%) Flow rate:. ml/min Detection (DAD): nm Instrument: Berger Analytical SFC Sample:. Dimethylphthalate. Caffeine. Uracil. Cortisone 9..... 6 7 8 9 Highly controlled chemistry of monolayer formation and end-capping Extremely high column-to-column reproducibility High selectivity and efficiency for separations Suitable for separations of acidic, neutral and basic organic compounds, and pharmaceuticals Sepax SFC-Cyano Products Column: SFC-Cyano (µm, Å,.6xmm) Mobile phase: % Acetonitrile/% H Flow rate:. ml/min Detector (UV): nm Injection volume:. µl Temperature: Ambient ( o C) Sample:. -Cyanophenol. Anisole. Benzophenone The SFC-Cyano packing materials are bonded with propyl cyano functional groups that enable special interaction with polar compounds. The monomeric bonding chemistry produces very high efficiency and high resolution separations. Sepax SFC-Cyano Pore size P/N m,.6xmm Å 6-6 m,.6xmm Å 6-6 m,.6xmm Å 6-6 m,.xmm Å 6- m,.xmm Å 6- m,.xmm Å 6- m, xmm Å 6- m, xmm Å 6- m, xmm Å 6- www.sepax-tech.com -877-SEPAX-US

AU Sepax SFC-Amino Si Si Silica: Spherical, high purity (< ppm metals) Pore size: Å Particle size:,, 7 and m Pore volume:. ml/g Surface area: m /g Phase structure: Polymeric and no endcapping % Carbon:.% Coverage: ~.6 mol/m (CH ) NH Well controlled chemistry of polymeric monolayer High column-to-column reproducibility Utilized as both SFC and LC phases Versatile mobile phases: non-aqueous solvents, such as hexane/ethyl acetate and chloroform/methanol, and aqueous solutions Recommended for separations of pharmaceuticals, saccharides, nucleotides, and basic organic compounds Sepax SFC-Amino Products Sepax SFC-Amino Pore size P/N m,.6xmm Å 6-6 m,.6xmm Å 6-6 m,.6xmm Å 6-6 m,.xmm Å 6- m,.xmm Å 6- m,.xmm Å 6- m,.xmm Å 6- m, xmm Å 6- m, xmm Å 6- m, xmm Å 6- m, xmm Å 6-.. Peak N=, Sym=..... 6 8 6 8 Column: SFC-Amino (µm, Å,.6xmm) Mobile phase:.7% EtH/.% H /8% Isooctane Flow rate:. ml/min Detector (UV): nm Injection volume:. µl Temperature: Ambient ( o C) Sample:. Toluene. Benzanilide. p-nitroaniline www.sepax-tech.com 6-877-SEPAX-US

AU mau Sepax SFC-Silica Si H Suitable for separations in aqueous and non-aqueous mobile phases Suitable for separations of polar and basic organic compounds, such as vitamins, steroids, as well as pharmaceuticals SFC test chromatogram,, Silica: Spherical, high purity (< ppm metals) Pore size: Å Particle size:,, 7 and m Pore volume:. ml/g Surface area: m /g Phase structure: activated hydroxyl (-H) % Carbon: %, 9 8 7 6 (N=7,).7.6.... Peak N=,6 Sym=. 6 7 8 Column: Sepax SFC-Silica (µ,.6x mm) Mobile phase: C /MeH Gradient: t (min) 6 7 8 9 MeH(%) Flow rate:. ml/min Detection (DAD): nm Instrument: Berger Analytical SFC Sample:. Dimethylphthalate. Caffeine. Uracil. Cortisone 9.. 6 8 6 8 Column: SFC-Silica (µm, Å,.6xmm) Mobile phase:.7% EtH/.% H /8% Isooctane Flow rate:. ml/min Detector (UV): nm Injection volume:. µl Temperature: Ambient ( o C) Sample:. Toluene. Benzanilide. p-nitroaniline Activated silica surface Ultra high purity Enhanced mechanical stability Sepax SFC-Silica Products Sepax SFC-Silica Pore size P/N m,.6xmm Å 67-6 m,.6xmm Å 67-6 m,.6xmm Å 67-6 m,.xmm Å 67- m,.xmm Å 67- m,.xmm Å 67- m, xmm Å 67- m, xmm Å 67- m, xmm Å 67- www.sepax-tech.com 7-877-SEPAX-US

rdering Information.8 m Analytical Columns (length x ID mm) Phases.x.x.x.x.x.x SFC-Cyano ( Å) 6-6- 6-6- 6-6- SFC-NH ( Å) 6-6- 6-6- 6-6- SFC-SCX ( Å) 66-66- 66-66- 66-66- SFC-Pyridine (Å) 69-69- 69-69- 69-69- SFC-Diol ( Å) 66-66- 66-66- 66-66- SFC-Silica ( Å) 67-67- 67-67- 67-67-.8 m Analytical Columns (length x ID mm) Phases.x.x.6x.6x.6x.6x SFC-Cyano ( Å) 6-6- 6-6 6-6 6-6 6-6 SFC-NH ( Å) 6-6- 6-6 6-6 6-6 6-6 SFC-SCX ( Å) 66-66- 66-6 66-6 66-6 66-6 SFC-Pyridine (Å) 69-69- 69-6 69-6 69-6 69-6 SFC-Diol ( Å) 66-66- 66-6 66-6 66-6 66-6 SFC-Silica ( Å) 67-67- 67-6 67-6 67-6 67-6. m Analytical Columns (length x ID mm) Phases.x.x.x.x.x.x SFC-Cyano ( Å) 6-6- 6-6- 6-6- SFC-NH ( Å) 6-6- 6-6- 6-6- SFC-SCX ( Å) 66-66- 66-66- 66-66- SFC-Pyridine (Å) 69-69- 69-69- 69-69- SFC-Diol ( Å) 66-66- 66-66- 66-66- SFC-Silica ( Å) 67-67- 67-67- 67-67-. m Analytical Columns (length x ID mm) Phases.x.x.6x.6x.6x.6x SFC-Cyano ( Å) 6-6- 6-6 6-6 6-6 6-6 SFC-NH ( Å) 6-6- 6-6 6-6 6-6 6-6 SFC-SCX ( Å) 66-66- 66-6 66-6 66-6 66-6 SFC-Pyridine (Å) 69-69- 69-6 69-6 69-6 69-6 SFC-Diol ( Å) 66-66- 66-6 66-6 66-6 66-6 SFC-Silica ( Å) 67-67- 67-6 67-6 67-6 67-6 m Analytical Columns (length x ID mm) Guard column Phases.x.x.x.x.x.x * SFC-Cyano ( Å) 6-6- 6-6- 6-6- SFC-NH ( Å) 6-6- 6-6- 6-6- SFC-SCX ( Å) 66-66- 66-66- 66-66- SFC-Pyridine (Å) 69-69- 69-69- 69-69- SFC-Diol ( Å) 66-66- 66-66- 66-66- SFC-Silica ( Å) 67-67- 67-67- 67-67- www.sepax-tech.com 8-877-SEPAX-US

m Analytical Columns (length x ID mm) Guard column Phases.6x.6x.6x.6x.6x.x * SFC-Cyano ( Å) 6-6 6-6 6-6 6-6 6-6 6- SFC-NH ( Å) 6-6 6-6 6-6 6-6 6-6 6- SFC-SCX ( Å) 66-6 66-6 66-6 66-6 66-6 66- SFC-Pyridine (Å) 69-6 69-6 69-6 69-6 69-6 69- SFC-Diol ( Å) 66-6 66-6 66-6 66-6 66-6 66- SFC-Silica ( Å) 67-6 67-6 67-6 67-6 67-6 67- m Analytical Columns (length x ID mm) Guard column Phases.x.x.x.x.x.x * SFC-Cyano ( Å) 6-6- 6-6- 6-6- SFC-NH ( Å) 6-6- 6-6- 6-6- SFC-SCX ( Å) 66-66- 66-66- 66-66- SFC-Pyridine (Å) 69-69- 69-69- 69-69- SFC-Diol ( Å) 66-66- 66-66- 66-66- SFC-Silica ( Å) 67-67- 67-67- 67-67- m Analytical Columns (length x ID mm) Guard column Phases.6x.6x.6x.6x.6x.x * SFC-Cyano ( Å) 6-6 6-6 6-6 6-6 6-6 6- SFC-NH ( Å) 6-6 6-6 6-6 6-6 6-6 6- SFC-SCX ( Å) 66-6 66-6 66-6 66-6 66-6 66- SFC-Pyridine (Å) 69-6 69-6 69-6 69-6 69-6 69- SFC-Diol ( Å) 66-6 66-6 66-6 66-6 66-6 66- SFC-Silica ( Å) 67-6 67-6 67-6 67-6 67-6 67- m preparative and semi-preparative Columns (length x ID mm) Guard column Phases 7.8x 7.8x x x.x * SFC-Cyano ( Å) 6-78 6-78 6-6- 6- SFC-NH ( Å) 6-78 6-78 6-6- 6- SFC-SCX ( Å) 66-78 66-78 66-66- 66- SFC-Pyridine ( Å) 69-78 69-78 69-69- 69- SFC-Diol ( Å) 66-78 666-78 66-66- 66- SFC-Silica ( Å) 67-78 67-78 67-67- 67- m preparative and semi-preparative Columns (length x ID mm) Guard column Phases.x.x.x.x.x.x ** SFC-Cyano ( Å) 6-6- 6-6- 6-6- SFC-NH ( Å) 6-6- 6-6- 6-6- SFC-SCX ( Å) 66-66- 66-66- 66-66- SFC-Pyridine (Å) 69-69- 69-69- 69-69- SFC-Diol ( Å) 66-66- 66-66- 66-66- SFC-Silica ( Å) 67-67- 67-67- 67-67- www.sepax-tech.com 9-877-SEPAX-US

m preparative and semi-preparative Columns (length x ID mm) Guard column Phases x x x x x.x ** SFC-Cyano ( Å) 6-6- 6-6- 6-6- SFC-NH ( Å) 6-6- 6-6- 6-6- SFC-SCX ( Å) 66-66- 66-66- 66-66- SFC-Pyridine (Å) 69-69- 69-69- 69-69- SFC-Diol ( Å) 66-66- 66-66- 66-66- SFC-Silica ( Å) 67-67- 67-67- 67-67- m preparative and semi-preparative Columns (length x ID mm) Guard column Phases 7.8x x x x.x * SFC-Cyano ( Å) 69-78 69-69- 69-69- SFC-NH ( Å) 69-78 69-69- 69-69- SFC-SCX ( Å) 669-78 669-669- 669-669- SFC-Pyridine ( Å) 699-78 699-699- 699-699- SFC-Diol ( Å) 669-78 669-669- 669-669- SFC-Silica ( Å) 679-78 679-679- 679-679- m preparative and semi-preparative Columns (length x ID mm) Guard column Phases.x.x.x.x.x.x ** SFC-Cyano ( Å) 69-69- 69-69- 69-69- SFC-NH ( Å) 69-69- 69-69- 69-69- SFC-SCX ( Å) 669-669- 669-669- 669-669- SFC-Pyridine (Å) 699-699- 699-699- 699-699- SFC-Diol ( Å) 669-669- 669-669- 669-669- SFC-Silica ( Å) 679-679- 679-679- 679-679- m preparative and semi-preparative Columns (length x ID mm) Guard column Phases x x x x x.x ** SFC-Cyano ( Å) 69-69- 69-69- 69-69- SFC-NH ( Å) 69-69- 69-69- 69-69- SFC-SCX ( Å) 669-669- 669-669- 669-669- SFC-Pyridine (Å) 699-699- 699-699- 699-699- SFC-Diol ( Å) 669-669- 669-669- 669-669- SFC-Silica ( Å) 679-679- 679-679- 679-679- * Guard column cartridge does not include the cartridge holder ** Guard column cartridge does not include the cartridge holder * Cartridge holder ** Cartridge holder P/N# -, $./EA for x mm guard cartridge P/N# -, $./EA for x mm guard cartridge P/N# -, $7/EA for x. mm guard cartridge Cartridge Holder Cartridge www.sepax-tech.com -877-SEPAX-US

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