Product Datasheet CD133 Antibody NB120-16518 Unit Size: 0.1 mg Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Publications: 8 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: www.novusbio.com/nb120-16518 Updated 3/18/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at www.novusbio.com/publications Submit a review at www.novusbio.com/reviews/destination/nb120-16518
NB120-16518 CD133 Antibody Product Information Unit Size Concentration Storage Clonality Preservative Isotype Purity Buffer 0.1 mg 1.0 mg/ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Polyclonal 0.02% Sodium Azide IgG Immunogen affinity purified PBS Page 1 of 4 v.20.1 Updated 3/18/2018 Product Description Host Rabbit Gene ID 8842 Gene Symbol Species Reactivity Notes Marker Specificity/Sensitivity Immunogen PROM1 Human, Mouse, Rat, Porcine Reacts with human, mouse and rat. porcine reactivity reported in scientific literature (PMID: 29352176). Stem Cell Marker CD133 - Hematopoietic Stem Cell Marker Synthetic peptide corresponding to a C-terminal region of CD133 (within amino acids 750-865). Product Application Details Applications Recommended Dilutions Application Notes Western Blot, Chromatin Immunoprecipitation, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin Western Blot 1:500-1:1000, Chromatin Immunoprecipitation, Flow Cytometry 1:10-1:1000, ELISA 1:1000, Immunohistochemistry 1:100-1:250, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry- Paraffin 1:100-1:250, Immunohistochemistry-Frozen ELISA: 1/1000. WB: 1/1,000-1:2,000. Detects a band of approximately 120 kda (predicted molecular weight: 97 kda). IHC: Citrate buffer antigen retreival required. ICC/IF: Fix with 3% paraformaldehyde for 20 min at RT. Optimal dilutions/concentrations should be determined by the end user. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 29352176).
Images Flow Cytometry: CD133 Antibody [NB120-16518] - Flow Cytometry: CD133 Antibody [DyLight 488] [NB120-16518G] - Flow Cytometry: CD133 Antibody (DyLight488) [NB120-16518G] - Rat bone marrow cells were stained with CD133 (1:100) antibody (20 minutes at 4C), fixed, and then analyzed. Image using the DyLight 488 form of this antibody. Page 2 of 4 v.20.1 Updated 3/18/2018 Western Blot: CD133 Antibody [NB120-16518] - Predicted band size : 97 kda recognizes 97 kda human CD133 in Y79 cells. Band is at ~120 kda due to protein glycosylation. Immunohistochemistry-Paraffin: CD133 Antibody [NB120-16518] - Staining of CD133 in human hepatocarcinoma (HC) tissue. NB120-16518 was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Chromatin Immunoprecipitation: CD133 Antibody [NB120-16518] - [NB 120-16518] - Staining CD133 in Bone Marrow Mononuclear Cells by Immunofluorescence.
Publications Page 3 of 4 v.20.1 Updated 3/18/2018 Zou X, Kwon SH, Jiang K et al. Renal scattered tubular-like cells confer protective effects in the stenotic murine kidney mediated by release of extracellular vesicles Sci Rep 2018 Jan 19 [PMID: 29352176] (IHC-Fr, Porcine) Alhaider IA, Mohamed ME, Ahmed KKM, Kumar AHS. Date Palm (Phoenix dactylifera) Fruits as a Potential Cardioprotective Agent: The Role of Circulating Progenitor Cells Front Pharmacol 2017 Sep 20 [PMID: 28928656] (Flow, Rat) Calle AS, Nair N, Oo AK et al. A new PDAC mouse model originated from ipscs-converted pancreatic cancer stem cells (CSCcm). Am J Cancer Res. Jan 2 2017 12:00AM [PMID: 28042501] (IHC, Mouse) Wang J, Liu X, Jiang Z et al. A novel method to limit breast cancer stem cells in states of quiescence, proliferation or differentiation: Use of gel stress in combination with stem cell growth factors Oncol Lett 2016 Aug 01 [PMID: 27446437] Lubeseder-Martellato C, Hidalgo-Sastre A, Hartmann C et al. Membranous CD24 drives the epithelial phenotype of pancreatic cancer. Oncotarget May 17 2016 12:00AM [PMID: 27203385] Wang K, Xu J, Zhang J, Huang J. Prognostic role of CD133 expression in colorectal cancer: a meta-analysis BMC Cancer 2012 Dec 5 [PMID: 23216926] (IHC, Human) Grosse-Gehling P, Fargeas CA, Dittfeld C et al. CD133 as a biomarker for putative cancer stem cells in solid tumours: limitations, problems and challenges J Pathol 2012 Aug 16 [PMID: 22899341] (ELISA, ICC/IF, IHC, WB, Human) Ma, T, Liu, H, Chen, W et al. Implanted Adipose-Derived Stem Cells Attenuate Small-for-Size Liver Graft Injury by Secretion of VEGF in Rats American Journal of Transplantation 2012 [PMID: 22151301] (FLOW, ICC/IF, Rat)
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