2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Issue No: 002 Issue date: 05 March 2018 Berkshire and Surrey Pathology Services Department of Histopathology Wexham Park Hospital Slough Berkshire SL2 4HL Contact: Elaine Inglis Tel: +44 (0) 1753 633098 E-Mail: Elaine Inglis1@nhs.net Website: www.nhspathology.fph.nhs.uk Testing performed at the above address only DETAIL OF ACCREDITATION Fixed, fresh tissue; excisional and incisional biopsies and surgical resection specimens Morphological assessment and interpretation/diagnosis LP-CP- Specimen Dissection HCPC 77 As appropriate to specimen type: Breast General Specimens GIT & Liver Head & neck Skin Soft tissue and bone Urology Specimen dissection & tissue processing HIS-WPH-SOP-0100 In conjunction with manufacturers instructions for Sakura VIP 5 & 6 tissue processors. Tissue embedding HIS-WPH-SOP-0100 In conjunction with manufacturers instructions for Sakura TEC 5 and Leica EG1150 embedding stations. Assessment Manager: GD1 Page 1 of 9
Formalin fixed praffin wax blocks of processed tissue Tissue sectioning HIS-WPH-SOP-0204 Microtome sectioning procedure In conjunction with manufacturers instructions for Leica RM2235, Microm 335, 330 microtomes Fresh frozen human tissue samples Diagnostic frozen sections HIS-WPH-SOP-0300 In conjunction with manufacturers instructions for Bright Clinicut and Bright OFT 5000 cryostats. above above Routine staining for identification of basophilic and eosinophilic structures Special staining to identify the following: Mast Cells Acid mucins Neutral Mucins Acid & Neutral mucins Helicobactor pylori Haematoxylin and Eosin staining using HIS-WPH-SOP-0217 and Operation of the Tissue-Tek Prisma Staining Machine & G2 Glass Coverslipper in conjunction with manufacturers instructions for Sakura Prisma and Sakura DRS 2000 automated staining machines. And HIS-WPH-SOP-0400 Documented in house procedures for manual specials stains HIS-WPH-SOP-0400 Toluidine Blue Alcian Blue PAS & Diastase LP-CP-Alcian Blue / Periodic Acid Schiff Modified Giemsa Assessment Manager:GD1 Page 2 of 9
above Special staining to identify the following: Elastic fibres Gram +/- organisms Fungi Haemosiderin Documented in house procedures for manual specials stains HIS-WPH-SOP-0400 Millers Elastic van Gieson Gram Grocott Iron (Perls Prussian Blue) Melanin Melanin Bleach Connective tissue Fibrin Hepatitis B & copper associated Protein PAS +ve substances e.g. Glycogen Reticulin fibres Metachromatic substances Calcium Tubercle bacilli (TB) Amyloid Masson Fontana Melanin Bleach Masson Trichrome Martius Scarlet Blue (MSB) Orcien (Shikata) Periodic acid Schiff Reticulin (Gordon & Sweet) Toluidine Blue Von Kossa Ziehl Neelsen Congo Red Assessment Manager:GD1 Page 3 of 9
above Pan Cytokeratin Prostate tumour marker Foetal Liver Scattered T cells in germinal centres & mantle zone B cells Germinal centre B Cells Epithelial Cells Luminal cells of the fallopian tube Parafolicular C cells of the thyroid Peripheral nerve ganglion T-Cells All germinal cells of tonsil Neutrophils, eosinophils, macrophages, R-S cells in Hodgkins lymphoma Germinal mantle zone B lymphocytes Activated lymphocytes & plasma cells. R-S cells in Hodgkins lymphoma following antibodies AE1/AE3 AMACR AFP BCL-2 BCL-6 BER-EP4 CA125 Calcitonin Calretinin CD5 CD10 CD15 CD20 CD30 Assessment Manager:GD1 Page 4 of 9
above Endothelial cells All leucocytes Peripheral nerves following antibodies CD34 CD45 CD56 Mantle zone B cells, germinal centre B cells, plasma cells GIST marker Carcinoma, plasma and epithelial cells Large cell anaplastic lymphoma Normal and neoplastic GIT epithelial cells Adenocarcinoma Neuroendocrine cells Squamous epithelium Ductal and glandular epithelium Squamous epithelium Intestinal Epithelium Squamous epithelium CD79A CD117 CD138 CD246 CDX2 CEA Chromogranin A CK5/6 CK7 CK14 CK20 CK34BE12 Assessment Manager:GD1 Page 5 of 9
above Epithelial cells Mantle cell lymphoma following antibodies CK MNF116 Cyclin D1 Endothelial Cells D2-40 Hairy Cell leukaemia Smooth & striated muscle cells Gastrointestinal stromal tumours (GIST) Epstein Barr virus infected cells Epithelial cell membranes Epithelial cells Breast and Urothelial Carcinoma Gestational trophoblastic cells Normal and neoplastic hepatocytes Hepatitis infected cells Melanoma cells Granulosa cell tumours and sertoli- Leydig cells tumours Light chains DBA-44 Desmin DOG-1 EBI-3 E-Cadherin EMA GATA3 HCG Hepatocyte Hepatitis Surface Antigen HMB45 Inhibin Kappa Assessment Manager:GD1 Page 6 of 9
above Proliferating cells Light chains Melanocytes lympho-hemopoietic neoplasms Rhabdomyosarcomas Neuroendocrine & neuronal cells Breast cancer and normal gland Colon adenocarcinoma Trophoblasts in placenta Squamous epithelium Renal Cell, Thyroid and Ovarian Carcinomas Germ cell tumours Breast cancer Prostate glands Peripheral nerve cells, Adipocytes, melanocytes & Histiocytes Smooth Muscle Actin following antibodies KI67 Lambda Melan A Mum-1 Myogenin NSE Oestrogen P53 P57 P63 PAX8 PLAP Progesterone PSA S100 SMA Assessment Manager:GD1 Page 7 of 9
above Slides prepared from human body tissues and fluids as above. Smooth Muscle Myosin Neural / neuroendocrine tumours Lymphoblastic leukaemia Follicular epithelial cells of thyroid Follicular epithelial cells of thyroid, type II pneumocytes, smooth muscle, endothelium lymphocytes Intermediate filaments Endothelial cells Mesenchymal cells Morphological assessment and interpretation/diagnosis following antibodies SMM Synaptophysin TdT Thyroglobulin TTF-1 Vimentin Von Willebrand WT-1 Interpretive microscopy Nikon Microscope Olympus Microscope HIS-WPH-SOP-0703 Assessment Manager:GD1 Page 8 of 9
Urine, nipple discharge, cyst fluid, skin scrapings, synovial fluid, CSF, sputum, bronchial washings, pleural fluid, peritoneal ascites, pericardial fluid, FNA solid organ, endoscopic US guided FNA and transbronchial FNA. Slides prepared from human body tissues and fluids as above. Cytopathology (Non-Gynae) examination activities for the purpose of clinical diagnosis Morphological assessment and interpretation/diagnosis examination HIS-WPH-SOP-0703 Preparation of Non-Gnynae Cytology Samples In conjunction with the manufacturers instructions for Sakura DRS 2000 staining machine. Interpretive microscopy Nikon Microscope Olympus Microscope HIS-WPH-SOP-0703 as appropriate to specimen type END Assessment Manager:GD1 Page 9 of 9