CARB 1004 Aldehyde/Ketone-DNPH Calibration

Reference Standards CARB 1004 Aldehyde/Ketone-DNPH Calibration Catalog # 33093 Lot # A0103403 110 Benner Circle Bellefonte, PA 16823-8812 1-814-353-1300 1-800-356-1688 www.restek.com FOR LABORATORY USE ONLY. READ SDS PRIOR TO USE. RAW MATERIAL TEST INFORMATION AVAILABLE UPON REQUEST MANUFACTURED UNDER RESTEK'S ISO 9001 REGISTERED QUALITY SYSTEM Page 1 of 8

CERTIFIED REFERENCE MATERIAL 110 Benner Circle Bellefonte, PA 16823-8812 Tel: (800)356-1688 Fax: (814)353-1309 Certificate of Analysis www.restek.com FOR LABORATORY USE ONLY-READ SDS PRIOR TO USE. This Reference Material is intended for Laboratory Use Only as a standard for the qualitative and/or quantitative determination of the analyte(s) listed. Catalog No. : 33093 Lot No.: A0103403 Description : CARB 1004 Aldehyde/Ketone-DNPH Calibration Aldehyde/Ketone-DNPH Calibration Std 3µg/mL, Acetonitrile, 1mL/ampul Container Size : Pkg Amt: Expiration Date : May 31, 2017 Storage: 0 C or colder C E R T I F I E D V A L U E S Elution Order Compound Grav. Conc. (weight/volume) 1 Formaldehyde-DNPH CAS # 1081-15-8 (Lot 130412JLM) Purity 14.3% 2 Acetaldehyde-DNPH CAS # 1019-57-4 (Lot 130625JLM) Purity 19.6% 3 Acrolein-DNPH CAS # 888-54-0 (Lot 130819JLM) Purity 23.8% 4 Acetone-DNPH CAS # 1567-89-1 (Lot 130307JLM) Purity 24.4% 5 Propionaldehyde-DNPH CAS # 725-00-8 (Lot 091102JLM) Purity 24.4% 6 Crotonaldehyde-DNPH CAS # 1527-96-4 (Lot 121031JLM) Purity 28% 7 Methyl ethyl ketone-dnph CAS # 958-60-1 (Lot 140106JLM) Purity 27.2% Expanded Uncertainty (95% C.L.; K=2) +/- 0.0420 µg/ml +/- 0.1443 µg/ml +/- 0.1464 µg/ml +/- 0.0420 µg/ml +/- 0.1442 µg/ml +/- 0.1463 µg/ml +/- 0.1437 µg/ml +/- 0.1458 µg/ml +/- 0.1438 µg/ml +/- 0.1460 µg/ml +/- 0.1438 µg/ml +/- 0.1460 µg/ml +/- 0.0420 µg/ml +/- 0.1443 µg/ml +/- 0.1464 µg/ml +/- 0.0418 µg/ml +/- 0.1434 µg/ml +/- 0.1455 µg/ml Page 2 of 8

8 Methacrolein-DNPH CAS # 5077-73-6 (Lot 002113) Purity 28% 9 n-butyraldehyde-dnph CAS # 1527-98-6 (Lot 101104JLM) Purity 28.6% 10 Benzaldehyde-DNPH CAS # 1157-84-2 (Lot 120203A) Purity 37% 11 n-valeraldehyde-dnph CAS # 2057-84-3 (Lot 120203JLM) Purity 32.3% 12 m-tolualdehyde-dnph CAS # 2880-05-9 (Lot 002119) Purity 40% 13 n-hexaldehyde-dnph CAS # 1527-97-5 (Lot 002112) Purity 35.7% +/- 0.0420 µg/ml +/- 0.1443 µg/ml +/- 0.1464 µg/ml +/- 0.1439 µg/ml +/- 0.1460 µg/ml +/- 0.0418 µg/ml +/- 0.1436 µg/ml +/- 0.1458 µg/ml +/- 0.1440 µg/ml +/- 0.1461 µg/ml +/- 0.1438 µg/ml +/- 0.1459 µg/ml +/- 0.0421 µg/ml +/- 0.1446 µg/ml +/- 0.1467 µg/ml Solvent: Acetonitrile CAS # 75-05-8 Purity 99% Specific Reference Material Notes: The reported concentrations reflect the amount of aldehyde or ketone in the mixture. The concentration of derivatized aldehyde/ketone is not reported. This certificate, updated 12/15/2016 for the following reason, supersedes any pre-existing certificate: Corrected the elution order of Acrolein-DNPH and Acetone-DNPH. Page 3 of 8

Column: 150mm x 4.6mm Ultra C18 (cat.# 9174565) Flow Rate: 1.5 ml/min. Mobile Phase A: water Mobile Phase B: acetonitrile Mobile Phase Composition: 0'-60%B:7'-70%B:9'-90%B:12'-100%B: 20'-100% Det. Type: Wavelength: 365 nm This chromatogram represents a general set of testing conditions chosen for product acceptance. For optimal results in your lab, conditions should be adjusted for your specific instrument, method, and application. Date Mixed: 15-May-2014 Balance: 1127510105 Date Passed: 19-May-2014 Manufactured under Restek's ISO 9001:2008 Registered Quality System Certificate #FM 80397 Page 4 of 8

General Certified Reference Material Notes Expiration Notes: Expiration date valid for unopened ampul stored in compliance with the recommended conditions. Uncertainty, concentration, and expiration of the CRM are based on the unopened product being stored according to the recommended condition found in the storage field. Purity Notes: Purity and/or chemical identity are determined by one or more of the following techniques: GC/FID, HPLC, GC/µECD, GC/MS, LC/MS, RI, and/or melting point. Compounds with a listed purity of less than 99% have been weight corrected to compensate for impurities and/or salts. A correction factor is used to calculate the amount of compound necessary to achieve the desired concentration of the parent compound in solution. Purity of isomeric compounds is reported as the sum of the isomers. Purity values are rounded to the nearest whole number. Certified Uncertainty Value Notes: The uncertainties are determined in accordance with ISO Guides 34 and 35. The certified combined stressed uncertainty value ( includes gravimetric uncertainty, homogeneity between-ampul uncertainty, storage stability uncertainty and shipping stability uncertainty and were combined using the following formula: k is a coverage factor of 2, which gives a level of confidence of approximately 95%. It is important to note that the shipping stability uncertainty was obtained under temperature extremes for specific time intervals; therefore, the certified combined stressed uncertainty value should only be applied to the product if it was stored at non-standard temperature conditions up to and including 7 days. Contact Restek Technical Service at www.restek.com/contact-us for use recommendations if your shipment was in-transit for more than 7 days at nonstandard temperature conditions. Apply the certified combined unstressed uncertainty value if the product was received under standard shipping conditions. Apply the certified combined stressed uncertainty value if the product was received under non-standard conditions as specified below. Label Conditions 25 C Nominal (Room Temperature) 10 C or colder (Refrigerate) 0 C or colder (Freezer) Standard Conditions < 60 C < 40 C < 25 C Non-Standard Conditions 60 C up to 7 days 40 C up to 7 days 25 C up to 7 days Separate (not combined) uncertainty values for gravimetric uncertainty are also displayed on the certificate, if needed, separate homogeneity between-ampul uncertainty, storage stability uncertainty and shipping stability uncertainty values are available by contacting Restek Technical Service at www.restek.com/contact-us. The packaged amount is the minimum sample size for which uncertainty is valid. The ampules are over-filled to ensure that the minimum packaged amount can be sufficiently transferred. Manufacturing Notes: Concentration is based upon gravimetric preparation using either a balance whose calibration has been verified daily using NIST traceable weights, and/or dilutions with Class A glassware. Handling Notes: Samples should be transferred into deactivated vials for handling and storage. Restek supplies deactivated vials along with most standards packed in 2 ml ampules. Due to space constraints, Restek does not supply vials for larger volume ampules. Restek sells DMDCS for the purpose of glassware deactivation as catalog number 31861, which includes complete instructions. Restek will also deactivate larger volume vials from our inventory as a custom ordered item. Contact your Restek sales or customer service representative for details. If any undissolved material is visible inside the ampul, sonicate the unopened ampul until the material is completely dissolved. Page 5 of 8

Catalog #: 33093 Target: 3 ug/ml Description: CARB 1004 Aldehyde/Ketone-DNPH Calibration Solvent: Acetonitrile Solvent Lot: 53248 Chemical Standard Batch Sheet Lot #:A0103403 Final Volume: 50 ml Made by: Alexandria Pavkovich Date: 5/15/2014 10:27:35PM Tested by: Amanda Miller Pass Date: 5/16/2014 12:36:02PM By: Diane Shaffer Date: 5/19/2014 10:01:11AM Packaged by: Lane Kibe / Lane Kibe Date: 5/15/2014 11:05:39PM No. Units: 32 Pkg Size: 1 Balance Used: BEDEARMBALPC7 XP205 Serial #: 1127510105 Compound CAS Formaldehyde-DNPH 1081-15-8 Acetaldehyde-DNPH 1019-57-4 Acetone-DNPH 1567-89-1 Acrolein-DNPH 888-54-0 Propionaldehyde-DNPH 725-00-8 Crotonaldehyde-DNPH 1527-96-4 Benzaldehyde-DNPH 1157-84-2 n-valeraldehyde-dnph 2057-84-3 m-tolualdehyde-dnph 2880-05-9 n-hexaldehyde-dnph 1527-97-5 n-butyraldehyde-dnph 1527-98-6 Methacrolein-DNPH 5077-73-6 Methyl ethyl ketone-dnph 958-60-1 Storage Target Location Lot # Purity Conc(ug/mL) Target Actual Calc Conc(ug/mL) F0231 A0103357 0.14 3.00 5.00 ml 5.00 ml 3.0 F0252 A0103357 0.20 3.00 5.00 ml 5.00 ml 3.0 F0253 A0103357 0.24 3.00 5.00 ml 5.00 ml 3.0 F0254 A0103357 0.24 3.00 5.00 ml 5.00 ml 3.0 F0255 A0103357 0.24 3.00 5.00 ml 5.00 ml 3.0 F0256 A0103357 0.28 3.00 5.00 ml 5.00 ml 3.0 F0258 A0103357 0.37 3.00 5.00 ml 5.00 ml 3.0 F0259 A0103357 0.32 3.00 5.00 ml 5.00 ml 3.0 F0261 A0103357 0.40 3.00 5.00 ml 5.00 ml 3.0 F0263 A0103357 0.36 3.00 5.00 ml 5.00 ml 3.0 F0264 A0103357 0.29 3.00 5.00 ml 5.00 ml 3.0 R0973 A0103357 0.28 3.00 5.00 ml 5.00 ml 3.0 R0974 A0103357 0.27 3.00 5.00 ml 5.00 ml 3.0 Page 6 of 8

Catalog #: 33093STK Target: 30 ug/ml Description: CARB 1004 Aldehyde/Ketone-DNPH Calib Stock Solvent: Acetonitrile Solvent Lot: 33248 Chemical Standard Batch Sheet Lot #:A0103357 Final Volume: 200 ml Made by: Alexandria Pavkovich Date: 5/15/2014 8:05:59PM Tested by: Date: By: Packaged by: / Date: No. Units: Balance Used: BEDEARMBALPC7 XP205 Serial #: 1127510105 Date: Pkg Size: 1 Compound CAS Formaldehyde-DNPH 1081-15-8 Acetaldehyde-DNPH 1019-57-4 Acetone-DNPH 1567-89-1 Acrolein-DNPH 888-54-0 Propionaldehyde-DNPH 725-00-8 Crotonaldehyde-DNPH 1527-96-4 Benzaldehyde-DNPH 1157-84-2 n-valeraldehyde-dnph 2057-84-3 m-tolualdehyde-dnph 2880-05-9 n-hexaldehyde-dnph 1527-97-5 n-butyraldehyde-dnph 1527-98-6 Methacrolein-DNPH 5077-73-6 Methyl ethyl ketone-dnph 958-60-1 Storage Target Location Lot # Purity Conc(ug/mL) Target Actual Calc Conc(ug/mL) F0231 130412JLM 0.14 30.00 41.96 mg 42.10 mg 30.1 F0252 130625JLM 0.20 30.00 30.61 mg 30.70 mg 30.1 F0253 130307JLM 0.24 30.00 24.59 mg 24.60 mg 30.0 F0254 130819JLM 0.24 30.00 25.21 mg 25.20 mg 30.0 F0255 091102JLM 0.24 30.00 24.59 mg 24.60 mg 30.0 F0256 121031JLM 0.28 30.00 21.43 mg 21.50 mg 30.1 F0258 120203A 0.37 30.00 16.22 mg 16.20 mg 30.0 F0259 120203JLM 0.32 30.00 18.58 mg 18.60 mg 30.0 F0261 002119 0.40 30.00 15.00 mg 15.00 mg 30.0 F0263 002112 0.36 30.00 16.81 mg 16.90 mg 30.2 F0264 101104JLM 0.29 30.00 20.98 mg 21.00 mg 30.0 R0973 002113 0.28 30.00 21.43 mg 21.50 mg 30.1 R0974 140106JLM 0.27 30.00 22.06 mg 22.00 mg 29.9 Page 7 of 8

QA Report: CARB 1004 Aldehyde/Ketone-DNPH Calibration (Cat.#33093) Runs of Lot # A087218 Runs of Lot # A0103403 COMPONENT Run #1 Run #2 Run #3 AVG STD DEV % RSD Run #1 Run #2 Run #3 AVG STD DEV % RSD %D MEAN P/F Formaldehyde-DNPH 353380 351997 352579 352652 694 0.20 351893 353091 352486 352490 599 0.17 0.05 PASS Acetaldehyde-DNPH 281414 281508 280820 281247 373 0.13 283836 283644 283952 283811 156 0.05-0.91 PASS Acetone-DNPH 478838 479884 479539 479420 533 0.11 476389 476870 477926 477062 786 0.16 0.49 PASS Acrolein-DNPH 218452 218825 218950 218742 259 0.12 218017 218770 218996 218594 513 0.23 0.07 PASS Propionaldehyde-DNPH & Crotonaldehyde-DNPH 214229 214621 214305 214385 208 0.10 213776 214240 214465 214160 351 0.16 0.10 PASS Methyl ethyl ketone-dnph 208886 209896 208149 208977 877 0.42 211920 213712 213620 213084 1009 0.47-1.97 PASS Methacrolein-DNPH 170747 171982 169813 170847 1088 0.64 179194 180374 179808 179792 590 0.33-5.24 PASS Butyraldehyde-DNPH 176900 174612 179206 176906 2297 1.30 177467 175688 177985 177047 1205 0.68-0.08 PASS Benzaldehyde-DNPH 141375 141920 141790 141695 285 0.20 141580 141259 142167 141669 460 0.33 0.02 PASS Valeraldehyde-DNPH 149079 149707 149588 149458 334 0.22 151319 151353 152423 151698 628 0.41-1.50 PASS m-tolualdehyde-dnph 119241 120542 120238 120007 681 0.57 118965 119536 119727 119409 396 0.33 0.50 PASS Hexaldehyde-DNPH 129182 129657 129083 129307 307 0.24 128281 128697 129007 128662 364 0.28 0.50 PASS Page 8 of 8 5/19/2014 9:49:42 AM 1/1-1