Page 1 of 1 RFE MEMO Product Description(s): V-PLEX and V-PLEX Plus Proinflammatory Panel 2 (rat) Kit Catalog Number(s) / Size(s): K15059-series Lot Number: K0080651 Based on additional testing, the original date of expiration for this kit has been extended by 12 months. The expiration date of K0080651 has been extended to 31 Jul 2018. The expiration dates for the following kit components have also been extended: Original Expiration Description Lot Number Date New Expiration Date Proinflammatory Panel 2 (rat) Plate Z0046287 30 Apr 2019 Same as Original Anti-rat IFN-γ Antibody D0080844 30 Apr 2019 Same as Original Anti-rat IL-1β Antibody D0080845 30 Apr 2019 Same as Original Anti-rat IL-4 Antibody D0080696 31 Jul 2018 Same as Original Anti-rat IL-5 Antibody D0080698 31 Jul 2018 Same as Original Anti-rat IL-6 Antibody D0080846 30 Apr 2019 Same as Original Anti-rat KC/GRO Antibody D0080847 30 Apr 2019 Same as Original Anti-rat IL-10 Antibody D0080848 30 Apr 2019 Same as Original Anti-rat IL-13 Antibody D0080671 30 Apr 2018 31 Jul 2018 Anti-rat TNF-α Antibody D0080849 30 Apr 2019 Same as Original Proinflammatory Panel 1 (rat) Calibrator Blend A0080083 31 May 2021 Same as Original Blocker H R0100010 28 Feb 2018 31 Jul 2018 Diluent 42 M0420008 28 Feb 2018 31 Jul 2018 Diluent 40 M0400009 31 Jul 2017 31 Jul 2018 Function Name Signature Date Quality Assurance Dawn Stollar 02 MAR 2017 This memo supersedes all other statements regarding date of expiration for this product. Memo Rev. A CM-00289-B
Page 1 of 5 Product Description: V-PLEX and V-PLEX Plus Proinflammatory Panel 2 (rat) Kit Kit Catalog Numbers: K15059-Series Kit Lot Number: K0080651 Expiration Date: 31 Jul 2017 Acceptance Data/ Percent Recovery Specifications: Calibrator Proinflammatory Panel 1 (rat) Control 1 Proinflammatory Panel 1 (rat) Control 2 Proinflammatory Panel 1 (rat) Control 3 Spot Name Recovery CV Recovery CV Recovery CV Recovery CV 1 rt IFN-γ 80% - 120% < 20% 75% - 125% < 25% 75% - 125% < 25% 75% - 125% < 25% 2 rt IL-1β 80% - 120% < 20% 75% - 125% < 25% 75% - 125% < 25% 75% - 125% < 25% 4 rt IL-4 80% - 120% < 20% 75% - 125% < 25% 75% - 125% < 25% 75% - 125% < 25% 5 rt IL-5 80% - 120% < 20% 75% - 125% < 25% 75% - 125% < 25% 75% - 125% < 25% 6 rt IL-6 80% - 120% < 20% 75% - 125% < 25% 75% - 125% < 25% 75% - 125% < 25% 7 rt KC/GRO 80% - 120% < 20% 75% - 125% < 25% 75% - 125% < 25% 75% - 125% < 25% 8 rt IL-10 80% - 120% < 20% 75% - 125% < 25% 75% - 125% < 25% 75% - 125% < 25% 9 rt IL-13 80% - 120% < 20% 75% - 125% < 25% 75% - 125% < 25% 75% - 125% < 25% 10 rt TNF-α 80% - 120% < 20% 75% - 125% < 25% 75% - 125% < 25% 75% - 125% < 25% LLOQ ULOQ Spot Name Value Recovery CV Value Recovery CV 1 rt IFN-γ 39.7 75% - 125% < 25% 3750 75% - 125% < 25% 2 rt IL-1β 102 75% - 125% < 25% 8100 75% - 125% < 25% 4 rt IL-4 8.00 75% - 125% < 25% 723 75% - 125% < 25% 5 rt IL-5 82.0 75% - 125% < 25% 3000 75% - 125% < 25% 6 rt IL-6 96.9 75% - 125% < 25% 8550 75% - 125% < 25% 7 rt KC/GRO 21.7 75% - 125% < 25% 728 75% - 125% < 25% 8 rt IL-10 163 75% - 125% < 25% 15700 75% - 125% < 25% 9 rt IL-13 12.5 75% - 125% < 25% 1080 75% - 125% < 25% 10 rt TNF-α 9.10 75% - 125% < 25% 793 75% - 125% < 25%
Page 2 of 5 Acceptance Data/ Non Specific Binding Specifications: Spot Name NSB Spec 1 rt IFN-γ < 1.0% 2 rt IL-1β < 1.0% 4 rt IL-4 < 1.0% 5 rt IL-5 < 1.0% 6 rt IL-6 < 1.0% 7 rt KC/GRO < 1.0% 8 rt IL-10 < 1.0% 9 rt IL-13 < 1.0% 10 rt TNF-α < 1.0% Stock Concentration of Material Tested : Spot Name Item Conc Units Calibrator 6300 1 rt IFN-γ Proinflammatory Panel 1 (rat) Control 1 4313 Proinflammatory Panel 1 (rat) Control 2 1049 Proinflammatory Panel 1 (rat) Control 3 517 Calibrator 13700 2 rt IL-1β Proinflammatory Panel 1 (rat) Control 1 12400 Proinflammatory Panel 1 (rat) Control 2 7940 Proinflammatory Panel 1 (rat) Control 3 1820 Calibrator 1310 4 rt IL-4 Proinflammatory Panel 1 (rat) Control 1 951 Proinflammatory Panel 1 (rat) Control 2 312 Proinflammatory Panel 1 (rat) Control 3 81.5 Calibrator 9280 5 rt IL-5 Proinflammatory Panel 1 (rat) Control 1 3380 Proinflammatory Panel 1 (rat) Control 2 1730 Proinflammatory Panel 1 (rat) Control 3 770 Calibrator 12200 6 rt IL-6 Proinflammatory Panel 1 (rat) Control 1 4783 Proinflammatory Panel 1 (rat) Control 2 1807 Proinflammatory Panel 1 (rat) Control 3 434 7 rt KC/GRO Calibrator 4060
Page 3 of 5 7 rt KC/GRO 8 rt IL-10 9 rt IL-13 10 rt TNF-α Proinflammatory Panel 1 (rat) Control 1 554 Proinflammatory Panel 1 (rat) Control 2 327 Proinflammatory Panel 1 (rat) Control 3 158 Calibrator 26100 Proinflammatory Panel 1 (rat) Control 1 19800 Proinflammatory Panel 1 (rat) Control 2 4360 Proinflammatory Panel 1 (rat) Control 3 1640 Calibrator 1670 Proinflammatory Panel 1 (rat) Control 1 1580 Proinflammatory Panel 1 (rat) Control 2 495 Proinflammatory Panel 1 (rat) Control 3 162 Calibrator 1340 Proinflammatory Panel 1 (rat) Control 1 803 Proinflammatory Panel 1 (rat) Control 2 282 Proinflammatory Panel 1 (rat) Control 3 86.9 Controls tested may not be the same lot as shipped.
Page 4 of 5 Standard Curve Data: rt IFN-γ - Spot 1 6300 1,530,000 3.9 1580 425,000 7.2 394 90,100 6.3 98.4 24,200 8.7 24.6 5,680 4.3 6.15 1,940 5.9 1.54 796 5.2 0.00 474 6.8 rt IL-1β - Spot 2 13700 1,220,000 2.6 3430 220,000 3.1 856 37,600 2.7 214 8,000 2.4 53.5 2,200 2.5 13.4 752 4.3 3.34 413 5.2 0.00 276 5.8 rt IL-4 - Spot 4 1310 1,110,000 2.4 328 302,000 1.9 81.9 57,900 1.6 20.5 11,500 1.4 5.12 2,750 3.2 1.28 955 3.5 0.320 511 6.6 0.00 352 5.8 rt IL-5 - Spot 5 9280 1,610,000 3.2 2320 491,000 2.6 580 83,700 5.2 145 10,200 3.5 36.3 1,440 3.5 9.06 492 5.3 2.27 376 5.3 0.00 334 5.4 rt IL-6 - Spot 6 12200 299,000 2.5 3050 62,700 2.4 763 14,000 3.5 191 3,410 2.4 47.7 1,190 5.1 11.9 560 7.1 2.98 413 8.3 0.00 371 14.0 rt KC/GRO - Spot 7 4060 1,120,000 3.5 1020 544,000 2.2 254 133,000 2.4 63.4 29,100 4.3 15.9 8,260 3.4 3.96 2,800 3.4 0.991 1,400 8.8 0.00 1,030 11.0 rt IL-10 - Spot 8 26100 911,000 4.1 6530 245,000 3.3 1630 61,700 3.6 408 16,100 4.7 102 4,340 3.8 25.5 1,640 12.0 6.37 877 11.0 0.00 737 20.0 rt IL-13 - Spot 9 1670 1,150,000 2.1 418 212,000 2.2 104 33,600 2.4 26.1 6,190 2.2 6.52 1,730 5.7 1.63 688 15.0 0.408 448 11.0 0.00 416 20.0 rt TNF-α - Spot 10 1340 923,000 3.5 335 209,000 2.4 83.8 42,700 2.0 20.9 9,740 2.3 5.23 3,120 5.1 1.31 1,240 10.0 0.327 987 16.0 0.00 762 18.0
Page 5 of 5 Components: Description Lot Number Storage Temperature Expiration Date Proinflammatory Panel 2 (rat) Plate Z0046287 2-8 C 30 Apr 2019 Anti-rat IFN-γ Antibody D0080844 2-8 C 31 Jul 2018 Anti-rat IL-1β Antibody D0080845 2-8 C 31 Jul 2018 Anti-rat IL-4 Antibody D0080696 2-8 C 31 Jul 2018 Anti-rat IL-5 Antibody D0080698 2-8 C 31 Jul 2018 Anti-rat IL-6 Antibody D0080846 2-8 C 30 Apr 2019 Anti-rat KC/GRO Antibody D0080847 2-8 C 30 Apr 2019 Anti-rat IL-10 Antibody D0080848 2-8 C 30 Apr 2019 Anti-rat IL-13 Antibody D0080671 2-8 C 30 Apr 2018 Anti-rat TNF-α Antibody D0080849 2-8 C 30 Apr 2019 Proinflammatory Panel 1 (rat) Calibrator Blend A0080083 2-8 C 31 May 2021 Blocker H R0100010 Room Temperature 28 Feb 2018 Diluent 42 M0420008-10 C 28 Feb 2018 Diluent 40 M0400009-10 C 31 Jul 2017 MSD Read Buffer T N/A Additional Components Included in V-PLEX Plus Plate Seals Wash Buffer (20X), 100mL Proinflammatory Panel 1 (rat) Control 1 Proinflammatory Panel 1 (rat) Control 2 Proinflammatory Panel 1 (rat) Control 3 Statement: The above product is intended for Research Use Only. Not for use in Diagnostic Procedures. Name Function Signature Date Review/Approval Dawn Stollar Quality Assurance 02 MAR 2017