Mark Scheme (Results) Summer Pearson Edexcel GCE In Biology (6BI04) Paper 01 The Natural Environment and Species Survival

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Mark Scheme (Results) Summer 2017 Pearson Edexcel GCE In Biology (6BI04) Paper 01 The Natural Environment and Species Survival

Edexcel and BTEC Qualifications Edexcel and BTEC qualifications come from Pearson, the world s leading learning company. We provide a wide range of qualifications including academic, vocational, occupational and specific programmes for employers. For further information, please visit our website at www.edexcel.com. Our website subject pages hold useful resources, support material and live feeds from our subject advisors giving you access to a portal of information. If you have any subject specific questions about this specification that require the help of a subject specialist, you may find our Ask The Expert email service helpful. www.edexcel.com/contactus Pearson: helping people progress, everywhere Our aim is to help everyone progress in their lives through education. We believe in every kind of learning, for all kinds of people, wherever they are in the world. We ve been involved in education for over 150 years, and by working across 70 countries, in 100 languages, we have built an international reputation for our commitment to high standards and raising achievement through innovation in education. Find out more about how we can help you and your students at: www.pearson.com/uk Summer 2017 Publications Code 6BI04_01_1706_MS All the material in this publication is copyright Pearson Education Ltd 2017

General Marking Guidance All candidates must receive the same treatment. Examiners must mark the first candidate in exactly the same way as they mark the last. Mark schemes should be applied positively. Candidates must be rewarded for what they have shown they can do rather than penalised for omissions. Examiners should mark according to the mark scheme not according to their perception of where the grade boundaries may lie. There is no ceiling on achievement. All marks on the mark scheme should be used appropriately. All the marks on the mark scheme are designed to be awarded. Examiners should always award full marks if deserved, i.e. if the answer matches the mark scheme. Examiners should also be prepared to award zero marks if the candidate s response is not worthy of credit according to the mark scheme. Where some judgement is required, mark schemes will provide the principles by which marks will be awarded and exemplification may be limited. When examiners are in doubt regarding the application of the mark scheme to a candidate s response, the team leader must be consulted. Crossed out work should be marked UNLESS the candidate has replaced it with an alternative response.

1(a) 1. chloroplast only : NB TWO structures needed for each mark grana, thylakoid (membrane / lumen), stroma, (inter granal) lamellae, starch {grains / granules}; 2. both chloroplasts and mitochondria : (double) membrane, ribosomes,; 3. mitochondria only : 2 Ignore cytoplasm Accept (loop) DNA 3 Ignore mesosomes matrix, stalked particles, {cristae / folded inner membrane} ; (3) 1(b)(i) 1. one glycerol and three fatty acids ; 2. reference to ester bonds (between fatty acids and glycerol); 3. idea that (triglycerides /fatty acids / hydrocarbon chains) may be saturated or unsaturated ; 3 Accept description of presence and absence of double carbon carbon bonds (3)

1(b)(ii) Answer 1(b)(ii). The only correct answer is A - condensation B is not correct because hydrolysis breaks bonds C is not correct because this is not an oxidation reaction Mark D is not correct because this is not a reduction reaction (1) 1(b)(iii) 1. use of reduced NADP produced by light-dependent reaction; 2. use of ATP produced by light-dependent reaction; 3.( light-independent reaction produces) {GALP / trioses} used in synthesis of {FAs / glycerol / triglyceride} ; 3 Accept GALP to glucose to glycerol 4. {GALP / trioses} converted to amino acids used to synthesise {proteins / enzymes} / eq ; 5. idea of enzymes used in synthesis of triglycerides ; (4)

2(a) 1. idea of suitable temperature for the bacteria to {grow / multiply / eq} ; Do not accept optimum temperature 2. idea of preventing the growth of (human) pathogens ; 2(b) 1. idea that Petri dish 1 plate opened with bacteria already on the agar ; 2. the risk of contamination (of the investigator) / eq ; 2(c) 1. P is bacteriostatic as the bacteria in Petri dish 1 were still present but prevented from growth in Petri dish 2 / eq ; Accept references to the presence or absence of clear zones as an eq for bacterial growth throughout 2. Q is bactericidal as bacteria did not grow in {either Petri dish}/ eq ; 3. {antibiotic R is not effective / bacteria are resistant to R} as they grew in both Petri dishes / eq ; (3)

3(a) Answer 3(a). The only correct answer is C role of a species in an ecosystem A is not correct because niche is about role not distribution and abundance B is not correct because niche is about role not location Mark D is not correct because niche is about role not trophic level (1) 3(b) 1. idea of testing water samples and {noting down observing / eq} (the different) species present ; Accept appropriate lab based experiment that includes all species being added to each 2. idea of using an (oxygen) probe / chemical testing kit ; concentration and survival looked for 3. idea that the lowest level of oxygen with species present is minimum level tolerated ; 3(c)(i) 4. idea that all other {variables / named variable} must be {controlled / monitored / eq} ; Answer 3(c)(i). The only correct answer is C species richness A is not correct because endemism describes organisms found in a specific area B is not correct because genetic diversity describes the genetic variation of a species (3) Mark D is not correct because taxonomy concerns classification (1)

Answer 3(c)(ii) 3(c)(ii). The only correct answer is C mayfly nymph Mark A is not correct because blood worm are found in polluted areas where the oxygen content will be low to avoid competition B is not correct because freshwater shrimp can tolerate low pollution levels and will therefore not be in unpolluted areas to avoid competition D is not correct because tubifex worms are found in polluted areas where the oxygen content will be low to avoid competition (1) 3(c)(iii) 1. idea that species adapted to {low oxygen levels / polluted water} will avoid competition ; 1 Accept converse 2. for {space / food / substratum / eq} ; 3. idea that there will be predators of some of the species ; 4. idea that the polluted water provided other {nutrients / food / eq} required by these species ; (3)

3(c)(iv) Answer 3(c)(iv). The only correct answer is D tubifex worm A is not correct because caddis fly need relatively high levels of oxygen so cannot survive in polluted water B is not correct because hoglouse need relatively high levels of oxygen so cannot survive in polluted water C is not correct because stone fly nymph need high levels of oxygen so cannot survive in polluted water Mark (1) 3(d)(i) idea that the {tube / syphon / eq} will absorb oxygen from the {air / above the water} ; Accept tail / flagellum Accept haemoglobin has a high affinity for oxygen / metabolism is very low /adapted to respire anaerobically (1) 3 (d)(ii) 1. anatomical / structural / physical ; 2. because it has the {tube / syphon / eq} ; 2 Accept tail / flagellum OR 3. behavioural ; 4. because it has to be close to the surface of the water / eq ;

4(a)(i) 1. (75% of 6100 =) 4575 (squirrels) ; (1) 4(a)(ii) 1. (Total number of alleles in black squirrels = 6100 2 =) 12200 ; Correct answer only gains full marks CE to be applied from 4(a)(i) 2. (4575 12200 =) 0.375 / 0.38 / 0.4; CE from mp 1 e.g.4575 6100 = 0.75 Accept 37.5 %

4(b) 1. idea that the allele for {black fur could increase / grey fur could decrease} if there was a disease ; Accept B will increase / b will decrease 2. because the disease would act as a selection pressure ; 3. idea that the black squirrels would survive and pass the black fur alleles onto their offspring ; 4. idea that black squirrels will out-compete the grey ones as they are more resistant to disease ; Accept B will be passed on... converse 5. idea that if the grey squirrels are wiped out the frequency of b will {decrease in the total population / stay the same in the black population} ; 6. idea that it will not change at all if there is no disease ; 7. idea that it will not change if the black squirrel is not immune to a particular disease that occurs; 8. idea that it will not change if the scientists were wrong ; (4)

*5(a)(i) (QWC Spelling of technical terms must be correct and the answer must be organised in a logical sequence) Emphasis is on logical sequence 1. idea that {vaccine / antigens / attenuated virus / eq } needs to be injected into person ; 1 Do not accept killing virus 2. to stimulate the (primary) immune response / eq ; 3. idea that macrophages present the antigen (in the vaccine) to T helper cells ; 4. idea that T helper cells release cytokines to stimulate {B cells / T killer cells} ; 5. resulting in formation of memory (T / B) cells ; 6. idea that memory cells remain in the body ; 7. idea that these memory cells are activated on infection (with the virus) ; 8. idea that the resulting immune response will be faster ; 8 e.g. idea of {faster / more} antibody produced (by plasma cells), idea of (macrophages) destroying virus before it has chance to replicate, idea that T killer cells would be able to destroy host-infected cells sooner (6)

Answer 5(a)(ii) 5(a)(ii). The only correct answer is A artificial active Mark B is not correct because a vaccine contains antigen and therefore stimulates an immune response C is not correct because a vaccine is not natural D is not correct because a vaccine contains antigen and therefore stimulates an immune response and is not natural (1) 5(a)(iii) 1. tested on animals ; 1 Accept cells, tissues 2. tested on (small number of) healthy individuals ; 3. tested on small number of people who are likely to come in contact with Zika ; 4. idea of (then) testing on a small number of pregnant women (to check for side effects in the baby) ; 5. idea that it is unlikely that phase III will be carried out first 3 Accept women before they got pregnant Do not accept people infected with the disease 4 Accept testing on pregnant animals (3)

5(b) 1. idea of analysing the structure of { RNA / protein / genetic material } from all three viruses ; NB penalyse reference to DNA only once 2. to determine the sequence of {bases in the RNA / amino acids in the protein} ; 3. reference to use of phylogenics ; 4. use of gel electrophoresis to analyse {RNA / proteins / eq} of the three viruses ; 3 Accept proteomics 4 Accept DNA made using RNA as a template 5. idea of closely-related viruses will have similar {protein / RNA} ; (3)

6(a) 1. idea that they can reduce the number of {(foreign) bacteria / fungi / yeast / pathogen} in the wound ; 2. by competing with them for {space / nutrients /eq} ; 1 Accept prevent colonisation by {(foreign) bacteria / fungi / yeast / pathogen} Do not accept virus 6(b)(i) 1. red / swollen ; 1 Ignore painful or hot 2. because of {increased blood flow to the area / histamine release /eq} ; 2 Accept vasodilation 6(b)(ii) 1. idea that it results in skin cells to {cover / close / repair / eq } wound ; 1 Accept replace the damaged cells 2. produce identical cells so that the (new) skin carries out the same { function / appearance / eq } ; 6(b)(iii) 1. idea of long (poly)peptide chains ; 1 Ignore long proteins 2. {little / no} tertiary structure / {hydrogen bonds / cross links} between (poly)peptide chains ; 2 Accept mainly secondary structure 3. idea of repeating amino acid sequences ;

6(c)(i) 1. idea that E. coli and Pseudomonas are found in all the ulcers ; 1 piece together 2. idea that Pseudomonas is the most common bacteria found in ulcers ; 3. idea that the presence of S. aureus depends on the type of ulcer ; 3 Accept e.g. of ulcers it does not contaminate, not found in all ulcers (3) 6(c)(ii) 1. idea that doctors cannot know which type of bacteria has caused the ulcer ; 1 Accept idea that ulcers are caused by a number of different types of bacteria 2. therefore {do not know which antibiotic will be effective / may prescribe the wrong antibiotic} ; 3. idea that a {wrong prescription of / broad spectrum} antibiotic can result in the increase in antibiotic resistance ; 2 Accept problem of prescribing only one antibiotic 3 Ignore unnecessary prescription Do not accept immune

7(a) 1. increase in {mean / average} temperature ; 2. of the earth's {surface / atmosphere} ; 7(b)(i) 1. idea that there is a correlation because when rainfall increases tree ring width increases 2. idea that in some years rainfall increases but tree ring width decreases, so not a perfect correlation ; 1 Accept idea that the peaks or troughs coincide / pattern of rainfall and tree ring width is similar 7(b)(ii) 1. use of water in { photolysis / light-dependent reaction } ; 2. credit a named molecule made by the plant and how it contributes to growth ; 3. credit use of water in transport of { sucrose / (mineral) ions } ; 4. credit named mineral ion and how it is related to growth ; 5. idea that the increase in tree ring growth results from an increase in {number / size} of xylem ; 2 e.g. glucose for energy, cellulose for new cells 3 Accept sugars, minerals, named mineral Ignore nutrients 4 e.g. nitrates used to make protein (3)

Answer 7(c)(i) 7(c)(i). The only correct answer is D - kj cm -2 yr -1 Mark A is not correct because an area is measured in cm 2 and it is per year B is not correct because an area is measured in cm 2 C is not correct because it is per year (1) 7(c)(ii) 1. idea that GPP is the { organic matter / eq } produced as a result of photosynthesis ; 1 Accept energy converted 2. idea that NPP is the { biomass / energy available for the next trophic level / eq } remaining after respiration ; 2 Accept NPP is the biomass and NPP=GPP-R 7(c)(iii) 1. idea that there are different species of tree ; 2. idea of genetic difference between the trees ; 3. idea that other parts of trees are growing not just tree rings ; 4. idea that another named abiotic variable involved ; 5. idea that a named biotic variable; 3 Accept number or size of leaves 4 e.g. temperature 5 e.g. disease, herbivores involved, competition (3)

*8(a) (QWC Spelling of technical terms must be correct and the answer must be organised in a logical sequence) Emphasis is on clarity of expression 1. idea that { bacteria / fungi / microorganisms } decompose (plant) material ; 2. reference to (release of) { enzymes / named enzyme } ; 1 Accept other named organism e.g. worm Ignore decomposers 3. reference to hydrolysis of {bonds / named bond} ; 4. credit example of plant molecule that is digested 4 e.g. starch, cellulose, protein OR credit example of named product of digestion ; 5. idea that some of the products are absorbed by the decomposers ; 6. credit use of products by the decomposer ; 5 Accept bacteria feed on these products 6 e.g. used in respiration 7. idea that {carbon dioxide / methane / eq} is released (by the decomposers) ; (6) 8(b)(i) 1. increase (in first 4 days) due to heat (energy) released by {respiration / metabolism} of decomposers / eq ; 2. decrease in temperature (after 4 days) due to { denaturation of enzymes / decrease in numbers of decomposers / decrease in substrate / eq } ; 2 Do not accept enzymes start to denature

8(b)(ii) 1. idea that decomposition would be faster; 2. as enzymes will work faster ; 3. idea that this would occur as long as the temperature was above ambient temperature ; 4. and below the optimum temperature of the enzymes ; 5. idea that {(core) temperature drop / algor mortis} would be slower ; 6. idea that {putrefaction / liquefaction } would be faster ; 7. idea that {maggots will hatch sooner / insect activity will be increased / eq } ; (5) Pearson Education Limited. Registered company number 872828 with its registered office at 80 Strand, London, WC2R 0RL, United Kingdom