Ultra High Performance Liquid Chromatograph Nexera X2 C196-E79
Maximizing the Potential of UHPLC/HPLC Analyses Intelligence Intelligent data solutions, including a new i -PDeA peak calculation method for enhanced separation power, accelerate workflows. Sensitivity The ultimate platform for high-sensitivity UHPLC and HPLC detection. Resolution Enhanced peak resolution achieved by minimizing external column dispersion. Stability Superior stability for UHPLC/HPLC analyses. Ul tra H ig h P e r for mance Liquid Chr oma to g ra p h
Utilizing patented technologies, and driven by customer input, Shimadzu has developed the Nexera X2 series to deliver superior, real-world performance across a wider application range, while delivering unparalleled flexibility and reliability in UHPLC/HPLC analyses. With no limits and no compromises, the Nexera X2 achieves a new milestone in the evolution of liquid chromatography.
Nexera X2 Series A full range of configurations to meet your applications needs Nexera SR system features superior sensitivity, resolution and stability. i-pdea* offers new separation technology. Nexera UHPLC/HPLC system enables switching from UHPLC to HPLC in a single system using a selection of mixers and columns. *Intelligent Peak Deconvolution Analysis Nexera Quaternary system is a flexible quaternary gradient system that is HPLC and UHPLC compatible. Nexera Method Scouting system offers comprehensive method development using 96 combinations of mobile phases and columns. Nexera MP system is an LC/MS frontend HPLC for high-throughput analysis of multiple samples. Nexera TQ system and Nexera SQ system offers ultrafast LC/MS and LC/MS/MS analysis. 4
Nexera X2 Component The CBM-2A enables system control via a network. The CBM-2A allows for various system configuration to meet different laboratory workflows. CBM-2A Detector SPD-M3A Ultra-high-sensitivity and high-resolution detector The SPD-M3A realizes ultra-high sensitivity and minimized dispersion, supporting the new separation calculation method, i-pdea. The temperature-controlled optics provide stable baseline for genuine UHPLC analysis. *Intelligent Peak Deconvolution Analysis Column oven CTO-3A Features control to a maximum 15 C (Max column length: 15 mm) CTO-2AC Large space for placing multiple columns and valves (Max column length: 3 mm) CTO-3AS Compact column oven for LC/MS front end (Max column length: 5 mm) Autosampler SIL-3AC Multifunctional autosampler; pretreatment and loop injection are available SIL-3AC / Rack Changer II Maximum 12 plates can be set with this combination SIL-3ACMP Open-access autosampler with maximum 6 plates Solvent delivery unit Upgradable to binary gradient and quaternary gradient, the LC-3AD also supports solvent blending for automated mobile phase preparation. LC-3AD Ultra High Performance Liquid Chromatograph 5
Intelligence in UHPLC/HPLC Analysis Complete Separation of Co-eluted Peaks by Intelligent Peak Deconvolution Analysis ( i-pdea) i-pdea enables the extraction of a single peak from co-eluted peaks by utilizing difference in spectra by a few steps. The new separation method removes discussion of integration methods for co-eluted peaks. The i-pdea also helps detect impurity peaks in a target peak. Step 1 Analysis Step 2 Extraction of single peak signal using the difference between each spectrum Step 3 Display of single component representative peak Component B AU Component A Component B 1 2 Component A 1 2 nm shows 1 extraction of component B. shows 2 extraction of component A. Baseline separation Vertical separation Removes peak integration ambiguities Example1: Remove tailing peak Tailing peak.5 mg/l spiked.488 mg/l measured (98% accuracy) Target peak i-pdea..25.5.75 1. 1.25 min The target peak is on the tailing peak..25.5.75 1. 1.25 min Remove the tailing peak!! Example2: Impurity peak extracted from co-eluted peak Peak purity curve i-pdea.22.24.26.28 min This peak looks a single peak..22.24.26.28 min By removing the main peak, the impurity peak appears on the chromatogram!! 6
Ultimate Sensitivity Achieving maximum sensitivity The SPD-M3A photodiode array detector adopts the high-sensitivity and low-dispersion capillary cell, SR-Cell, which achieves a.4 1-5 AU noise level. The SPD-M3A offers ultimate in UHPLC analysis, realizing high-sensitivity and high-resolution. High-sensitivity and high-resolution SR-Cell Section of capillary cell The SR-Cell features high-sensitivity and low-dispersion by adopting total reflection capillary cell. mau 175 μau Main peak 125 6 4 S/N 9.53 2 75 5.5 7.7 9.9 min Impurity peak 25..5 1. min Column Shim-pack XR-ODS (5 mml. 2. mmi.d., 1.6 μm).6 ml/min Main peak propyl 4-hydroxybenzoate.5 Impurity peak ethyl 4-hydroxybenzoate The excellent sensitivity and wide dynamic detection range enables simultaneous analysis of a main ingredient and impurity of.5% content. Column Time program Shim-pack VP-ODS (15 mml. 4.6 mmi.d., 4.6 μm) 2% ( min)1% (3 min) 1. ml/min. 5. 1. 15. 2. 25. min Column Time program Shim-pack XR-ODS (75 mml. 3. mmi.d., 2.2 μm) 2% ( min)1% (4.2 min) 1.3 ml/min. 1. 2. 3. 4. min The SR-Cell is available for UHPLC and HPLC, realizing high-sensitivity analysis across a wide analytical range. Ultra High Performance Liquid Chromatograph 7
Maximized Resolution Superior peak shape for maximum peak resolution In ultrafast analysis, external column dispersion affects peak resolution. The SR-Cell in the SPD-M3A has minimized the cell volume to realize excellent peak shape with higher peak resolution, supporting genuine UHPLC analysis. peak 1 peak 2 peak 3 Column Shim-pack XR-ODS III (5 mml. 2. mmi.d., 1.6 μm).6 ml/min Tailing factor (Tf5%) Peak No. Nexera X2 Vendor A Vendor A UHPLC 1 2 1.39 1.297 1.546 1.486 Nexera X2 3 1.273 1.432.2.25.3.35.4.45.5 min Superior spectrum resolution and linearity The SPD-M3A provides unrivaled spectrum resolution by adopting an optimized optic system. In addition, the new signal treatment technology realizes excellent spectrum linearity across a wide area from low concentration to high concentration. The SPD-M3A supports analyses, such as purity analysis, that require a wide dynamic range. B/A=2.9 AU Caffeine 1 mg/l Caffeine 1 mg/l B Similarity.9998 A 23 24 25 26 27 28 nm A Benzene spectrum is often used for evaluation of spectrum resolution. The SPD-M3A shows world s highest spectrum resolution, B/A=2.9*. 25 3 35 nm (Normalize) The SPD-M3A shows excellent spectrum linearity in the analysis of caffeine samples with different concentrations. Nexera SR system for high-sensitivity and high-resolution Nexera SR system is configured with the new SPD-M3A photodiode array detector. The new peak calculation technology, i-pdea, proposes a new approach for separation of co-eluted peaks and extraction of impurity peaks from main peaks. The superior sensitivity and resolution expand the range of UHPLC analyses. *As of November 212, according to Shimadzu research. Nexera SR system 8
Unrivaled Stability Baseline stabilization by temperature-controlled TC-Optics The SPD-M3A adopts the new temperature-controlled TC-Optics with the SR-Cell that has an optimized heat exchange inlet pipe. These features achieve faster stabilization times low external dispersion for UHPLC analysis. The faster stabilization shortens the wait time for analysis, thereby increasing total analytical throughput. mau 1 SPD-M3A Mobile phase.5 ml/min Methanol Detector PDA 254 nm -1 Temperature RT -2-3 -4 Vendor A PDA detector 1 2 3 4 5 min The SPD-M3A shows a stable baseline faster after startup while the detector from vendor A shows baseline drift during 1 hour. SPD-M3A Stable performance against room temperature shift The SPD-M3A incorporates TC-Optics to achieve a more stable baseline against room temperature shifts. mau 25 254 nm.5 ml/min 2 15 Room temperature 3 C Mobile phase Detector Methanol UV 254 nm 1 5 SPD-M3A 25 C -5-1 -15 Vendor A PDA detector -2 1 2 3 4 5 6 min Ultra High Performance Liquid Chromatograph 9
Ultimate Flexibility Automation of mobile phase preparation by solvent blending function The modular nature of Nexera X2 allows a solvent blending function that can mix solvents according to desired ratios. This function enables dilution of solvent, buffer preparation and modifier addition for mobile phase preparation. 85% 1% 5% % A B C D Pump1 5% A 2% 5% B C 25% D Pump2 Solvent Blending Automated mobile phase preparation Binary Gradient Gradient elution by prepared solvents Buffer ph can be optimized using the solvent blending function as shown to the right chromatograms. The function is a useful tool for optimizing mobile phase conditions in method development. Blending 1 1, 2, 3 A / B = 6 / 4 ph 3. Blending 2 2 3 A / B = 2 / 8 ph 7.2 1, Benzoic acid 2, Sorbic acid 3, methyl 4-hydroxybenzoate 1. 1. 2. 3. 4. min. 1. 2. 3. 4. min Measured ph of mobile phase prepared by solvent blending Solvent blending Blending 1 3.1 Blending 2 7.192 Manual preparation 3.4 7.191 Pump X solvent Blending 1 A / B = 6 / 4 Blending 2 A / B = 2 / 8 A; 2 mmol/l phosphoric acid aqueous solution B; 2 mmol/l disodium hydrogen-phosphate Pump Y solvent Methanol Combination of UHPLC / HPLC Switching system and solvent blending The solvent blending function can be added to a Nexera UHPLC/HPLC switching system by upgrading the configuration. The system supports all processes from method development to method transfer from HPLC to UHPLC. Nexera UHPLC/HPLC system 1
Enhanced UHPLC Reliability Ultrafast 7-second injection and 14-second cycle time Nexera X2 autosamplers support ultrafast injections to increase analytical throughput. In particular, the SIL-3ACMP achieves a 7-second injection time and a 14-second cycle time, making it ideally suited for ultrafast analysis. 125 1:235.4>86.1(+) 2:256.1>167.1(+) 3:281.1>86.1(+) 14 sec Column Shim-pack XR-ODS II (3 mml. 1.5 mmi.d., 2.2 μm) 1.2 ml/min 1 75 Event # Compound Q1 m/z Q3 m/z 1 Lidocaine 235.4 86.1 5 2 Diphenhydramine 256.1 167.1 25 3 Imipramine 281.1 86.1..25.5.75 1. min Minimizing carryover with multiple rinse solvents The Nexera X2 autosamplers, SIL-3AC and SIL-3ACMP, provide near-zero carryover. The improved design maintains a low carryover level, even during long-term usage, to ensure reliable analysis. AU 2 175 15 125 1 75 5 25 uv 75 5 25.18% 2.25 2.5 2.75 min Column Shim-pack XR-ODS III (15 mml. 2. mmi.d., 2.2 μm) Mobile phase Water / Methanol = 7 / 3.4 ml/min Sample Caffeine 4 mg/l The carryover level was extremely low even after 3, injections. -25..5 1. 1.5 2. 2.5 min Both the SIL-3AC and SIL-3ACMP support the use of multiple rinse solvents for rinsing the needle s outer surface and inner surface to thoroughly minimize carryover. Needle Sample loop Rinse ports Contact surfaces of needle and needle port where carryover is likely to occur Drain Needle port (schematic diagram) R R1 R2 R3 For rinsing needle surface and inner surface (3 types) For rinsing needle surface (1 type) Multi-rinse mechanism can accommodate up to 4 solutions Port section is rinsed by any of the rinse solutions (1 to 3 solutions) Discharge to drain port Ultra High Performance Liquid Chromatograph 11
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