PI3K / AKT / mtor. Signalling Pathway in Cancer. PI3K / AKT / mtor Related Antibodies from CovalAb

NEWSLETTER Version 1 From Research to Discovery PI3K / AKT / mtor Signalling Pathway in Cancer PI3K / AKT / mtor Related Antibodies from CovalAb www.covalab.com CovalAb - 11 avenue Albert Einstein 69100 VILLEURBANNE - France Tel: +33 (0) 437 654 230

Amongst the numerous pathways implicated in cancer development, the PI3K/AKT/mTOR signalling pathway is highly active in cancer cells. Mutation or amplification of the PI3-kinase or AKT genes, as well as loss of function of PTEN protein, result in the activation of the mtor pathway, which is associated with a poor prognosis in cancer, and therapy resistance. Antibodies 14-3-3 (60C10) Ms Hu, Ms, Rat IHC - P, WB mab71368 14-3-3 (C-Terminus) Rb Rat, Hu, Ms... IHC - P, WB pab70029 14-3-3 Rb Hu, Ms, Rat IHC - P, IP, WB pab74800 14-3-3 Beta (aa1-246) (J2E9) Ms Hu ELISA, IHC - P, WB mab70291 14-3-3 Beta (N-Terminus) Rb Hu, Ms, Rat IHC - P pab72731 14-3-3 Beta (N-Terminus) Rb Hu, Ms, Rat IHC - P, WB pab74861 14-3-3 Beta Rb Hu, Zfi ICC, IF, IHC - P pab73313 14-3-3 Beta Rb Hu, Zfi ICC, IF, IHC - P pab73314 14-3-3 Beta Rb Hu, Ms, Rat IHC - P, WB pab75102 14-3-3 Epsilon / YWHAE (aa1-255) (5A5) Ms Hu ELISA, IHC - P, WB mab70282 14-3-3 Eta / YWHAH (N-Terminus) Rb Chick, Hu, Ms IHC - P, WB pab74766 14-3-3 Gamma (KC21) Ms Hu, Ms, Rat WB mab50115 14-3-3 Gamma / YWHAG (aa1-247) (J3H10) Ms Hu ELISA, IHC - P, WB mab70294 14-3-3 Gamma (HS23) Ms Hu, Ms, Rat WB mab50036 14-3-3 protein Sigma (Stratifin) Rb Hu, Ms, Rat ELISA, IHC, WB pab0392 14-3-3 protein Sigma phosphoserine 248 (Stratifin-pSer248) Rb Hu ELISA, WB, IHC pab0713 14-3-3 protein Zeta/Delta phosphoserine 184 (KCIP-1/pSer184) Rb Hu ELISA, WB, IHC pab0644 14-3-3 Tau / YWHAQ (aa1-245) (AT1A1) Ms Hu ELISA, IHC - P, WB mab70297 14-3-3 Zeta Delta / YWHAZ (C-Terminus) Rb Hu, Ms, Rat IF, IHC - P, WB... pab74327 14-3-3 Zeta Delta / YWHAZ (Internal) Rb Hu, Ms, Rat ELISA, IHC - P, WB pab74404 14-3-3 Zeta Delta / YWHAZ (pser58) Rb Hu, Ms, Rat IHC - P, WB pab72083 AKT Rb Hu, Ms, Rat IHC - P, IP, WB pab75231 AKT1 (C-Terminus) Rb Hu IHC - P, WB pab72125 AKT1 (N-Terminus) Rb Hu, Ms, Rat IHC - P, WB pab70307 AKT1 (pser473) Rb Hu ELISA, IHC - P, WB pab70039 AKT1 (pthr308) Rb Hu ELISA, IHC - P, WB pab70040 AKT1 (pthr308) Rb Hu, Ms, Rat IHC - P, WB pab71951 AKT1 Rb Hu, Ms, Rat WB, IHC, IF... pab80597 AKT1 Rb Hu, Rat, Ms ELISA, WB, IHC pab0895-p AKT1 pser473 Rb Hu, Rat, Ms ELISA, WB pab0834-p AKT1 pthr308 Rb Hu, Rat, Ms ELISA, WB pab0832-p AKT1 pthr450 Rb Hu, Rat, Ms ELISA, WB pab0833-p AKT1/2/3 (Internal) Rb Hu, Ms, Rat ELISA, IHC - P, WB pab74277 AKT2 (1B6) Ms Hu, Mk, Rat IF, IHC - P, WB... mab70099 AKT3 (aa119-136) (66C1247.1) Ms Hu, Ms, Rat IHC - P, WB mab70131 AKT3 (aa119-136) Rb Hu, Ms, Rat IHC - P, WB pab74591 AKT3 (Internal) Goat Hu, Ms, Rat ELISA, IHC - P, WB pab72934 AKT3 (N-Terminus) Rb Hu, Ms IHC - P, WB pab70308 ATG13 (C-Terminus) Rb Hu, Ms, Rat ELISA, IHC - P, WB pab70353 14-3-3 Beta pab73313 14-3-3 Gamma mab50036 AKT1(pSer473) pab70039 Anti-14-3-3 Beta ICC staining (in green) of HeLa cells. Immunocytochemistry of paraformalhehydefixed cells. DAPI in blue. Anti-14-3-3 Gamma western blot staining of lane 1 : HeLa cell lysate lane 2 & 3 : bengamide treated (8h) cell lysate. Anti-AKT1 IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed paraffinembedded tissue after heat-induced antigen retrieval. www.covalab.com CovalAb - 11 avenue Albert Einstein 69100 VILLEURBANNE - France Tel: +33 (0) 437 654 230 1

REFERENCES 1. Yuan TL and Cantley LC. PI3K pathway alterations in cancer: variations on a theme. 2008. Oncogene 27:5497-510 2. Yang WL et al. Regulation of Akt signaling activation by ubiquitination. 2010. Cell Cycle 9:487-97 3. Zhao B. Inactivation of YAP oncoprotein by the Hippo pathway is involved in cell contact inhibition and tissue growth control. 2007. Genes Dev 21:2747-61 4. Sakamaki JI et al. Arginine methylation of BCL-2 antagonist of cell death (BAD) counteracts its phosphorylation and inactivation by Akt. 2011. PNAS 108:6085-90 5. Takahashi-Yanaga F and Sasaguri T. GSK-3beta regulates cyclin D1 expression: a new target for chemotherapy. 2008. Cell Signal 20:581-9 6. Wullschleger S et al. TOR signaling in growth and metabolism. 2006. Cell 124:471-84 7. De Benedetti A and Graff JR. eif-4e expression and its role in malignancies and metastases. 2004. Oncogene 23:3189-99 8. Wade M et al. The p53 orchestra: Mdm2 and Mdmx set the tone. 2010. Trends Cell Biol 20:299-309 9. Chipuk JE et al. Direct activation of Bax by p53 mediates mitochondrial membrane permeabilization and apoptosis. 2004. Science 303:1010-4 10. Fu Z and Tindall DJ. FOXOs, cancer and regulation of apoptosis. 2008. Oncogene 27:2312-9 www.covalab.com CovalAb - 11 avenue Albert Einstein 69100 VILLEURBANNE - France Tel: +33 (0) 437 654 230 ABBREVIATIONS Akt = protein kinase B; BAD = Bcl-2-associated death promoter; Bax = Bcl2-associated X protein; Bcl-2 = B-cell lymphoma protein-2; Bim = BH3-only protein; Cyclin D1 = G1/S-specific cyclin-d1; eif4ebp1 = eukaryotic translation initiation factor 4E-binding protein 1; FoxO1 = forkhead box protein O1; GAB2 = GRB2-associated-binding protein 2; Grb2 = growth factor receptor-bound protein 2; GSK3 = glycogen synthase kinase 3; IRS-1 = insulin receptor substrate 1; Mdm2 = mouse double-minute 2 protein; mlst8/gβl = mammalian lethal with SEC13 protein 8/G protein beta subunit-like; mtor = mammalian target of rapamycin; mtorc1 = mammalian target of rapamycin complex 1; mtorc2 = mammalian target of rapamycin complex 2; PDCD4 = programmed cell death protein 4; PDK1 = 3-phosphoinositide-dependent protein kinase 1; PI3K = phosphatidylinositol 3 kinase; PRR5 = proline-rich protein 5; PTEN = phosphatase and tensin homolog; p27kip1 = cyclin-dependent kinase inhibitor 1B; p53 = tumor protein 53; Rheb = Ras homolog enriched in brain; RTK = receptor tyrosine kinase; XIAP = X-linked inhibitor of apoptosis; YAP = Yes-Associated Protein/ Yorkie homolog. www.covalab.com CovalAb - 11 avenue Albert Einstein 69100 VILLEURBANNE - France Tel: +33 (0) 437 654 230 2

Antibodies BAD pab80585 Bim mab70713 CCND1 pab70552 Anti-BAD IF staining of rat thymus. Anti-BCL2L11/Bim IHC staining of human heart. Immunohistochemistry of formalinfixed, paraffin-embedded tissue after heatinduced antigen retrieval. Anti-Cyclin D1 IF staining (in green) of HeLa cells. Actin filaments in red. ATG13 (C-Terminus) Rb Hu, Ms, Rat WB, IHC, IF... pab81118 BAD (C-Terminus) Rb Hu, Ms, Rat WB, IHC, IF... pab80585 BAD (C-Terminus) Rb Hu, Ms, Rat IHC - P, WB pab74999 BAD (C-Terminus) Rb Hu, Ms, Rat ELISA, IHC - P, WB pab74258 BAD (Internal) Rb Hu, Ms, Rat ELISA, IHC - P pab74329 BAD (N-Terminus) (BYC001) Ms Ms, Hu IHC - P, IP, WB mab70326 BAD (pser112) Rb Hu, Ms, Rat IHC - P, WB pab71955 BAX (6A7) Ms Hu, Ms, Rat IHC - P, IP, WB mab71211 BAX (Internal) Rb Hu, Ms, Rat ELISA, IF, IHC - P pab73965 BAX (N-Terminus) Rb Ms, Hu, Rat IHC - P, IP, WB pab74913 BAX (N-Terminus) Rb Hu ICC, IHC - P, WB pab74874 BAX Rb Hu WB, ICC, IF... pab80303 BCL2 / Bcl-2 (11C5) Ms Hu IHC - P, IP, WB mab71374 BCL2 / Bcl-2 (aa41-54) Ms Hu FC, IF, IHC - P mab70328 BCL2 / Bcl-2 (Internal) Rb Hu, Ms, Rat IF, IHC - P, WB... pab73966 BCL2 / Bcl-2 (pser70) Rb Hu IHC - P pab71957 BCL2 / Bcl-2 (pthr56) Rb Hu IHC - P, WB pab71956 BCL2 / Bcl-2 Rb Hu IHC - P, WB pab75275 BCL2 Rb Hu ELISA, WB, ICC pab80216 BCL2 Rb Hu, Ms WB, IHC, IF... pab80584 Bim / BCL2L11 (Internal) Rb Hu, Ms, Rat ICC, IHC - P, WB pab70412 Bim / BCL2L11 (N-Terminus) Rb Hu, Ms, Rat IHC - P, WB pab74063 Bim Ms Hu, Ms, Rat WB, ICC, IF... mab80032 Bim Rb Hu, Ms, Rat ELISA, WB, IHC pab80416 Bim Rb Hu, Ms, Rat WB, ICC, IF... pab80307 CCND1 / Cyclin D1 (CD1.1) Ms Hu, Rat FC, ICC, IHC - F mab70713 CCND1 / Cyclin D1 (Ser90) Rb Hu, Ms IF, IHC - P, WB... pab70552 CDKN1B / p27 Kip1 (4B4-E6) Ms Hu IF, IHC - P, WB... mab70794 CDKN1B / p27 Kip1 (C-Terminus) Rb Hu, Ms, Rat IHC - P, IP, WB pab73599 CDKN1B / p27 Kip1 (C-Terminus) Rb Hu, Ms, Rat IHC - P, IP, WB pab74928 CDKN1B / p27 Kip1 (Internal) Goat Hu, Ms, Rat ELISA, IHC - P, WB pab72942 CDKN1B / p27 Kip1 (pthr187) Rb Hu ELISA, IHC - P, WB pab70627 CDKN1B / p27 Kip1 (pser10) Rb Hu, Ms, Rat IHC - P, WB pab72061 CDKN1B / p27 Kip1 Rb Hu IHC - P, WB pab74994 EIF4B (aa150-200) Rb Hu, Ms, Rat... IHC - P, WB pab74158 EIF4B (aa570-630) Rb Hu, Mk, Ms IHC - P, WB pab74169 EIF4B (pser422) Rb Hu ELISA, IHC - P, WB pab75290 www.covalab.com CovalAb - 11 avenue Albert Einstein 69100 VILLEURBANNE - France Tel: +33 (0) 437 654 230 3

Antibodies EIF4EBP1 pab70848 FOXO1 pab74237 mtor pab74323 Anti-EIF4EBP1/4EBP1 IHC staining of human prostate. Immunohistochemistry of formalin-fixed paraffin-embedded tissue after heat-induced antigen retrieval. Anti-FOXO1 western blot staining of HeLa cell lysate. Anti-MTOR IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffinembedded tissue after heat-induced antigen retrieval. EIF4E (C-Terminus) Rb Hu, Ms, Rat ELISA, IHC - P, WB pab74060 EIF4E (N-Terminus) Rb Hu IHC - P, WB pab72277 EIF4EBP1 / 4EBP1 (aa101-118) Rb Hu, Ms, Rat GS, IHC - P, WB pab72829 EIF4EBP1 / 4EBP1 (C-Terminus) Rb Hu ICC, IHC - P, WB pab70848 EIF4EBP1 / 4EBP1 (Internal) Rb Hu, Ms, Rat ELISA, IHC - P, WB pab74250 EIF4EBP2 / 4EBP2 (aa99-120) Rb Hu, Ms, Rat IHC - P, WB pab72830 EIF4ENIF1 (C-Terminus) Goat Hu, Ms, Rat ELISA, IHC - P, WB pab72332 FOXO1 (aa242-655) (7H3) Ms Hu IHC - P, WB mab70133 FOXO1 (C-Terminus) Goat Hu, Ms, Rat... IHC - P pab73015 FOXO1 (Internal) Rb Hu, Ms, Rat IF, IHC - P, WB... pab74237 FOXO1 (N-Terminus) Rb Hu, Ms, Rat WB, ICC, IF... pab81123 FOXO1 (N-Terminus) Rb Hu, Ms, Rat ICC, IHC - P, WB pab70979 FOXO1 (pser256) Rb Hu, Ms, Rat IHC, IHC - P, WB pab73083 GAB2 (Internal) Rb Hu, Ms, Rat ELISA, IHC - P, WB pab74281 Glycogen synthase kinase-3 Rb P. fals. ELISA, IHC, WB pab0250 Glycogen synthase kinase-3 Beta 2 Rb Hu ELISA, WB, IF pab0668 GRB2 (C-Terminus) Rb Hu, Ms, Rat IHC - P, WB pab73178 GRB2 (C-Terminus) Goat Hu, Ms, Rat ELISA, IHC - P, WB pab72922 GRB2 Rb Hu IHC - P, WB pab72987 GSK3B / GSK3 Beta (C-Terminus) Rb Hu, Ms, Rat IHC, IHC - P, WB pab75210 GSK3B / GSK3 Beta (pser9) Rb Hu GS, IF, IHC - P pab70152 GSK3B / GSK3 Beta Rb Rat, Hu, Ms IF, IHC - P, WB pab75234 IRS1 (aa1223-1242) Rb Hu, Ms, Rat IHC - P pab72749 IRS1 (C-Terminus) Rb Hu IHC - P, WB pab72768 IRS1 (Internal) Rb Hu, Ms, Rat ELISA, IF, IHC - P pab73796 IRS1 (Internal) Rb Hu, Ms ICC, IHC - P, WB pab71542 IRS1 Rb Hu, Ms WB, ICC, IF... pab80318 MDM2 (1A7) Ms Hu ELISA, IHC - P, WB mab70863 MDM2 (aa154-167) (SMP14) Ms Hu, Ms IHC, IHC - F, IP mab71411 MDM2 (aa177-195) Rb Ms ELISA, IHC - P, WB pab70162 MDM2 (pser185) Rb Ms ELISA, IHC - P, WB pab70163 MDM2 (pser395) Rb Hu ELISA, IHC - P pab71722 MDM4 / MDMX (2D10F4) Ms Hu ELISA, IF, IHC, WB mab70075 MLST8/ GBL (aa150-200) Rb Hu, Ms, Rat IHC - P, WB pab74502 MLST8/ GBL (C-Terminus) Rb Hu, Ms, Rat IHC - P, WB pab71043 mtor (aa2440-2457) Rb Hu, Ms, Rat IF, IHC - P, WB... pab70158 mtor (C-Terminus) Rb Hu, Ms, Rat ELISA, IHC - P, WB pab74323 www.covalab.com CovalAb - 11 avenue Albert Einstein 69100 VILLEURBANNE - France Tel: +33 (0) 437 654 230 4

Antibodies mtor (C-Terminus) Rb Hu, Ms, Rat ELISA, IHC - P, WB pab74267 mtor (N-Terminus) Rb Hu, Ms ICC, IHC - P, WB pab71788 mtor (pser2448) Rb Hu ELISA, IHC - P, WB pab70076 mtor Rb Hu, Ms ELISA, WB, IHC pab0894-p p53 / TP53 (Acetyl-Lys379) Rb Hu, Ms, Rat ELISA, IF, IHC - P pab73997 p53 / TP53 (C-Terminus) Rb Hu IF, IHC - P, WB... pab74272 p53 / TP53 (C-Terminus) Rb Hu, Ms, Rat IF, IHC - P, WB... pab74392 p53 / TP53 (C-Terminus) Rb Hu, Ms, Rat IF, IHC - P, WB... pab74391 p53 / TP53 (N-Terminus) Rb Hu, Rat ELISA, IHC - P, WB pab74269 p53 / TP53 (N-Terminus) Rb Hu, Ms, Rat IF, IHC - P, WB... pab74271 p53 / TP53 Ms Hu ELISA, IHC - P, IP, WB mab70600 p53 (H53C2) Ms Hu ELISA, WB, IHC, IP mab0052-p p53r2 Rb Hu, Ms, Rat WB, IHC, IF... pab80536 PDCD4 (aa1-469) (K4C1) Ms Hu ELISA, IHC - P, WB mab70283 PDCD4 (C-Terminus) Rb Hu, Ms, Rat ELISA, IHC - P, WB pab70157 PDCD4 (C-Terminus) Rb Hu, Ms, Rat IHC - P, WB pab72099 PDCD4 (C-Terminus) Rb Hu, Ms, Rat ELISA, WB, IHC pab80473 PDCD4 (pser457) Rb Hu, Ms, Rat ELISA, IHC - P, WB pab70146 PDCD4 Rb Hu, Ms, Rat IHC - P, WB pab75081 PHLPP1 (N-Terminus) Rb Hu ELISA, WB pab81508 PHLPP2 (C-Terminus) Rb Hu ELISA, WB pab81509 PI 3 Kinase p110 Delta (aa450-550) Rb Hu IHC - P, WB pab72240 PIK3C2A (3E7) Ms Hu ELISA, IHC - P, WB mab71053 PIK3CA (Internal) Goat Hu, Ms, Rat ELISA, IHC - P pab72400 Protor-1 / PRR5 (C-Terminus) Rb Hu, Ms, Rat WB, IHC, IF... pab81287 Protor-1 / PRR5 (C-Terminus) Rb Hu IHC - P, WB pab72514 Protor-2 / PRR5L Rb Hu ELISA, IHC, WB pab0500 Protor-2 / PRR5L Rb Hu, Ms WB, ICC, IF... pab81288 PRR5 (C-Terminus) Rb Hu IHC - P, WB pab72514 PRR5 (C-Terminus) Rb Hu, Ms, Rat WB, IHC, IF... pab81287 PTEN (C-Terminus) Rb Hu, Ms ELISA, WB pab80085 PTEN (N-Terminus) Rb Hu, Ms ELISA, WB, IF pab80388 PDK1 Rb Hu, Ms, Rat... IHC - P, WB pab75233 PDK1 Rb Hu ELISA, IHC - P, WB pab75048 Pyruvate dehydrogenase kinase (PDK1) [Biotin] Rb Hu WB pab50698 Pyruvate dehydrogenase kinase (PDK1) [Biotin] Rb Hu WB pab50692 Pyruvate dehydrogenase kinase (PDK1) [DyLight 488] Rb Hu WB pab50700 p53(h53c2) mab0052 PI3K p110 Delta pab72240 PDK1 pab75233 Immunoprecipitation of p53 mutated forms stably expressed in the p53 null HEp3B cell line: lane 1: Hep3B - lane 2: Hep3B143 lane 3: Hep3B248 - lane 4: Hep3B249. Anti-PI3 kinase p110 Delta IHC staining of human placenta, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heatinduced antigen retrieval. Anti-PDK1 western blot staining of lysate from: lane 1: MWM cell, lane 2: HeLa cell, lane 3: rat brain, lane 4: mouse brain, lane 5: Vero cell, lane 6: ESK-4 cell, lane 7: RK-13 cell, lane 8: MDCK cell. www.covalab.com CovalAb - 11 avenue Albert Einstein 69100 VILLEURBANNE - France Tel: +33 (0) 437 654 230 5

Antibodies Pyruvate dehydrogenase kinase (PDK1) [DyLight 488] Rb Hu WB pab50694 Pyruvate dehydrogenase kinase (PDK1) [DyLight 550] Rb Hu WB pab50702 Pyruvate dehydrogenase kinase (PDK1) [DyLight 550] Rb Hu WB pab50696 Pyruvate dehydrogenase kinase (PDK1) [DyLight 650] Rb Hu WB pab50699 Pyruvate dehydrogenase kinase (PDK1) [DyLight 650] Rb Hu WB pab50693 Pyruvate dehydrogenase kinase (PDK1) [HRP] Rb Hu WB pab50701 Pyruvate dehydrogenase kinase (PDK1) Rb Hu, Ms, Rat WB pab50211 Rheb (Internal) Rb Hu, Ms, Rat IHC - P, WB pab75117 Rheb (N-Terminus) Rb Hu, Ms, Rat ELISA, WB pab80082 Rheb Rb Hu, Ms, Rat IHC - P, WB pab72847 Rheb Rb Hu, Ms, Rat WB, IHC, IF pab80596 RICTOR (1F3) Ms Hu IHC - P, IP, WB mab71077 RICTOR (C-Terminus) Goat Hu, Ca, Ms ELISA, IHC - P pab74051 RPS6 (C-Terminus) Rb Hu, Ms, Rat IF, IHC - P, WB... pab73900 RPS6 (pser235) Rb Hu, Ms, Rat IHC - P, WB pab72108 RPS6KB1 / S6K (C-Terminus) Rb Hu, Ms, Rat IHC - P, IP, WB pab70130 RPS6KB1 / S6K (C-Terminus) Goat Hu, Ms, Rat ELISA, IHC - P, WB pab73148 RPS6KB1 / S6K (C-Terminus) Rb Hu, Ms, Rat ELISA, IHC - P, WB pab74280 RPS6KB1 / S6K (Internal) Rb Hu, Ms, Rat IF, IHC - P, WB... pab74275 RPS6KB1 / S6K (Internal) Rb Hu, Ms, Rat ELISA, IHC - P, WB pab74363 RPS6KB1 / S6K (pser411) Rb Hu, Ms, Rat IHC - P, WB pab71965 RPTOR / Raptor (aa900-950) Rb Hu, Ms IHC - P, WB pab74797 RPTOR / Raptor (N-Terminus) Rb Hu, Ms ICC, IHC - P, WB pab72900 RPTOR / Raptor Rb Hu, Ms ICC, IHC - P, WB pab72901 RPTOR / Raptor Rb Hu IHC - P, WB pab72114 Serine / threonine-protein Phosphatase 2A (PP2A) Ms Hu, Ms IP, WB mab50072 SGK1 (1C4) Ms Hu IF, IHC - P, WB... mab70910 SGK1 (3C4) Ms Hu IF, IHC - P, WB... mab70911 SGK1 (3E3) Ms Hu IF, IHC - P, WB... mab70909 SGK1 (3G8) Ms Hu IF, IHC - P, WB... mab70912 SGK1 (aa419-431) Rb Hu ELISA, IHC - P, WB pab70167 SGK1 (C-Terminus) Rb Hu, Ms, Rat WB, IHC, IF pab81299 SIN1 (aa100-150) Rb Hu, Ms, Rat IHC - P, WB pab73961 SIN1 (Internal) Rb Hu, Ms, Rat IHC - P, WB pab73206 SIN1 (N-Terminus) Rb Hu, Ms, Rat IHC - P, WB pab73207 PDK1 pab50211 Rheb pab80596 RPTOR pab72114 Anti-PDK1 western blot staining of human heart lysate. Anti-Rheb IF staining of mouse brain cells. Anti-RPTOR IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffinembedded tissue after heat-induced antigen retrieval. www.covalab.com CovalAb - 11 avenue Albert Einstein 69100 VILLEURBANNE - France Tel: +33 (0) 437 654 230 6

Antibodies TSC1 pab73745 VPS43 pab50333 Yorkie homolog pab0848-p Anti-TSC1 IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffinembedded tissue after heat-induced antigen retrieval. Anti-VPS34 western blot staining of HepG2 cell lysate. Anti-YAP1 homolog staining of HeLa cells expressing YAP1. SIN1 / MAPKAP1 (N-Terminus) Rb Hu, Ms, Rat WB, IHC, IF pab80667 SIN1 / MAPKAP1 Rb Hu, Ms, Rat ELISA, WB, IHC pab80481 THEM4 / CTMP (Internal) Rb Hu, Ms, Rat IHC, IHC - P, WB pab73519 THEM4 / CTPM Rb Hu, Ms, Rat WB, IHC, IF pab80905 TSC1 (C-Terminus) Rb Hu, Ms ELISA, WB pab80083 TSC1 (C-Terminus) Rb Hu, Ms IHC - P, WB pab73744 TSC1 (C-Terminus) Goat Hu, Ms, Rat ELISA, IHC - P, WB pab73166 TSC1 Rb Hu, Ms, Rat WB, ICC, IF... pab80315 TSC1 Rb Hu, Ms, Rat ICC, IHC - P, WB pab73745 TSC2 (C-Terminus) Rb Hu, Ms ELISA, WB pab80084 TSC2 (N-Terminus) Rb Hu, Ms WB, ICC, IF... pab80316 TSC2 / Tuberin (C-Terminus) Rb Hu, Ms IHC - P, WB pab73746 TSC2 / Tuberin (N-Terminus) Rb Hu, Ms ICC, IHC - P, WB pab73747 TSC2 / Tuberin (pser664) Rb Hu IF, IHC - P pab74899 TSC2 / Tuberin (pthr1462) Rb Hu IF, IHC - P pab74900 TSC2 / Tuberin (pser1798) Rb Hu ELISA, IHC - P, WB pab73749 VPS34 Rb Hu, Ms, Rat... WB pab50333 VPS34 [Biotin] Rb Hu WB pab51579 VPS34 [DyLight 488] Rb Hu WB pab51581 VPS34 [DyLight 550] Rb Hu WB pab51583 VPS34 [DyLight 650] Rb Hu WB pab51580 VPS34 [HRP] Rb Hu WB pab51582 XIAP (C-Terminus) Rb Hu, Ms IHC - P, WB pab74231 XIAP Rb Hu ICC, IF, IHC - P pab73363 XIAP Rb Hu WB pab51048 Yorkie homolog (YAP1) (2F12) Ms Hu IF, IHC - P, WB... mab70705 YAP1 (2H1) Ms Hu IF, IHC - P, WB... mab70706 YAP1 Rb Hu IHC - P, WB, IP pab50282 YAP1 [Biotin] Rb Hu IHC - P, IP, WB pab51306 YAP1 [DyLight 488] Rb Hu IHC - P, IP, WB pab51308 YAP1 [DyLight 550] Rb Hu IHC - P, IP, WB pab51310 YAP1 [DyLight 650] Rb Hu IHC - P, IP, WB pab51307 YAP1 [HRP] Rb Hu IHC - P, IP, WB pab51309 YAP1 Rb Hu WB, IHC, IF pab0848-p www.covalab.com CovalAb - 11 avenue Albert Einstein 69100 VILLEURBANNE - France Tel: +33 (0) 437 654 230 7

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