ACE ENVIRONMENTAL 49 SHADOWCREEK WAY ORMOND BEACH, FL 32174 Certificate of Mold Analysis Prepared for: ACE ENVIRONMENTAL Phone Number: (904) 859-9211 Fax Number: Project Name: PINNAMENENI Test Location: 11121 BRIDGE HOUSE DR WINDERMERE, FL 34786 Chain of Custody #: 874317 Received Date: August 11, 2015 Report Date: August 12, 2015 Erika Piechowski, Technical Manager Carlos Ochoa, Quality Control Manager Currently there are no Federal regulations for evaluating potential health effects of fungal contamination and remediation. This information is subject to change as more information regarding fungal contaminants becomes available. For more information visit http://www.epa.gov/mold or www.nyc.gov/html/doh/html/epi/mold.shtml. This document was designed to follow currently known industry guidelines for the interpretation of microbial sampling, analysis, and remediation. Since interpretation of mold analysis reports is a scientific work in progress, it may as such be changed at any time without notice. The client is solely responsible for the use or interpretation. PRO-LAB/SSPTM Inc. makes no express or implied warranties as to health of a property from only the samples sent to their laboratory for analysis. The Client is hereby notified that due to the subjective nature of fungal analysis and the mold growth process, laboratory samples can and do change over time relative to the originally sampled material. PRO-LAB/SSPTM Inc. reserves the right to properly dispose of all samples after the testing of such samples are sufficiently completed or after a 7 day period, whichever is greater. For more information please contact PRO-LAB at (954) 384-4446 or email info@prolabinc.com Page 1 of 17
0 Prepared for : ACE ENVIRONMENTAL Test Address : PINNAMENENI 11121 BRIDGE HOUSE DR WINDERMERE, FL 34786 ANALYSIS METHOD Direct Microscopic Exam Direct Microscopic Exam Spore trap analysis Spore trap analysis LOCATION STUDY - WALL BEHIND PANEL AIR HANDLER CLOSET #1 MASTER BATH LIVING ROOM COC / LINE # 874317-1 874317-2 874317-3 874317-4 SAMPLE TYPE & VOLUME SWAB TAPE Z5-25L Z5-25L SERIAL NUMBER SWAB TAPE Q284229 Q284224 COLLECTION DATE Aug 9, 2015 Aug 9, 2015 Aug 9, 2015 Aug 9, 2015 ANALYSIS DATE Aug 12, 2015 Aug 12, 2015 Aug 12, 2015 Aug 12, 2015 CONCLUSION UNUSUAL UNUSUAL ELEVATED ELEVATED IDENTIFICATION Aspergillus Bipolaris/Drechslera Cercospora Chaetomium Cladosporium Curvularia Ganoderma Mold Present X X Mold Present Hyphae X X Nigrospora X 1 2 Penicillium/Aspergillus 54 2, 96 236 9,0 100 Pithomyces Pyricularia Smuts, myxomycetes 1 2 Unidentified TOTAL SPORES NA NA 56 2,280 100 236 9,0 100 MINIMUM DETECTION LIMIT * NA NA 1 1 BACKGROUND DEBRIS Not Applicable Not Applicable Light Light Cellulose Fiber 4 160 4 160 Fiberglass Insect Fragments Plant Fragments Pollen OBSERVATIONS & COMMENTS Presence of current or former growth observed. Presence of current or former growth observed. Background debris qualitatively estimates the amount of particles that are not pollen or spores and directly affects the accuracy of the spore counts. The categories of Light, Moderate, Heavy and Too Heavy for Accurate, are used to indicate the amount of deposited debris. Increasing amounts of debris will obscure small spores and can prevent spores from impacting onto the slide. The actual number of spores present in the sample is likely higher than reported if the debris estimate is Heavy or Too Heavy for Accurate. All calculations are rounded to two significant figures and therefore, the total percentage of spore numbers may not equal 100%. * Minimum Detection Limit. Based on the volume of air sampled, this is the lowest number of spores that can be detected and is an estimate of the lowest concentration of spores that can be read in the sample. NA = Not Applicable. that were observed from the samples submitted are listed on this report. If a spore is not listed on this report it was not observed in the samples submitted. Interpretation Guidelines: A determination is added to the report to help users interpret the mold analysis results. A mold report is only one aspect of an indoor air quality investigation. The most important aspect of mold growth in a living space is the availability of water. Without a source of water, mold generally will not become a problem in buildings. These determinations are in no way meant to imply any health outcomes or financial decisions based solely on this report. For questions relating to medical conditions you should consult an occupational or environmental health physician or professional. CONTROL is a baseline sample showing what the spore count and diversity is at the time of sampling. The control sample(s) is usually collected outside of the structure being tested and used to determine if this sample(s) is similar in diversity and abundance to the inside sample(s). ELEVATED means that the amount and/or diversity of spores, as compared to the control sample(s), and other samples in our database, are higher than expected. This can indicate that fungi have grown because of a water leak or water intrusion. Fungi that are considered to be indicators of water damage include, but are not limited to: Chaetomium, Fusarium, Memnoniella, Stachybotrys, Scopulariopsis, Ulocladium. NOT ELEVATED means that the amount and/or the diversity of spores, as compared to the control sample and other samples in our database, are lower than expected and may indicate no problematic fungal growth. UNUSUAL means that the presence of current or former growth was observed in the analyzed sample. An abundance of spores are present, and/or growth structures including hyphae and/or fruiting bodies are present and associated with one or more of the types of mold/fungi identified in the analyzed sample. NORMAL means that no presence of current or former growth was observed in the analyzed sample. If spores are recorded they are normally what is in the air and have settled on the surface(s) tested. Page 2 of 17
0 Prepared for : ACE ENVIRONMENTAL Test Address : PINNAMENENI 11121 BRIDGE HOUSE DR WINDERMERE, FL 34786 ANALYSIS METHOD Spore trap analysis Spore trap analysis Spore trap analysis Spore trap analysis LOCATION STUDY ENTRYWAY/FOYER HALL BATH / POWDER RM HALL / AH CLOSET #1 & 2 COC / LINE # 874317-5 874317-6 874317-7 874317-8 SAMPLE TYPE & VOLUME Z5-25L Z5-25L Z5-25L Z5-25L SERIAL NUMBER Q284242 Q284196 Q284234 Q284230 COLLECTION DATE Aug 9, 2015 Aug 9, 2015 Aug 9, 2015 Aug 9, 2015 ANALYSIS DATE Aug 12, 2015 Aug 12, 2015 Aug 12, 2015 Aug 12, 2015 CONCLUSION ELEVATED ELEVATED ELEVATED ELEVATED IDENTIFICATION Aspergillus Bipolaris/Drechslera Cercospora Chaetomium Cladosporium 1 1 Curvularia Ganoderma Hyphae Nigrospora 1 1 1 1 1 1 Penicillium/Aspergillus 576 23,000 100 141 5,600 99 122 4,900 99 144 5,800 99 Pithomyces Pyricularia Smuts, myxomycetes 1 1 Unidentified TOTAL SPORES 576 23,000 100 143 5,680 100 123 4,9 100 146 5,880 100 MINIMUM DETECTION LIMIT * 1 1 1 1 BACKGROUND DEBRIS Light Light Light Light Cellulose Fiber 4 160 7 280 3 3 Fiberglass 1 2 80 1 Insect Fragments 1 Plant Fragments 4 160 Pollen OBSERVATIONS & COMMENTS too heavy for accurate count. s are estimated. Actual numbers of spores probably much higher. Background debris qualitatively estimates the amount of particles that are not pollen or spores and directly affects the accuracy of the spore counts. The categories of Light, Moderate, Heavy and Too Heavy for Accurate, are used to indicate the amount of deposited debris. Increasing amounts of debris will obscure small spores and can prevent spores from impacting onto the slide. The actual number of spores present in the sample is likely higher than reported if the debris estimate is Heavy or Too Heavy for Accurate. All calculations are rounded to two significant figures and therefore, the total percentage of spore numbers may not equal 100%. * Minimum Detection Limit. Based on the volume of air sampled, this is the lowest number of spores that can be detected and is an estimate of the lowest concentration of spores that can be read in the sample. NA = Not Applicable. that were observed from the samples submitted are listed on this report. If a spore is not listed on this report it was not observed in the samples submitted. Interpretation Guidelines: A determination is added to the report to help users interpret the mold analysis results. A mold report is only one aspect of an indoor air quality investigation. The most important aspect of mold growth in a living space is the availability of water. Without a source of water, mold generally will not become a problem in buildings. These determinations are in no way meant to imply any health outcomes or financial decisions based solely on this report. For questions relating to medical conditions you should consult an occupational or environmental health physician or professional. CONTROL is a baseline sample showing what the spore count and diversity is at the time of sampling. The control sample(s) is usually collected outside of the structure being tested and used to determine if this sample(s) is similar in diversity and abundance to the inside sample(s). ELEVATED means that the amount and/or diversity of spores, as compared to the control sample(s), and other samples in our database, are higher than expected. This can indicate that fungi have grown because of a water leak or water intrusion. Fungi that are considered to be indicators of water damage include, but are not limited to: Chaetomium, Fusarium, Memnoniella, Stachybotrys, Scopulariopsis, Ulocladium. NOT ELEVATED means that the amount and/or the diversity of spores, as compared to the control sample and other samples in our database, are lower than expected and may indicate no problematic fungal growth. UNUSUAL means that the presence of current or former growth was observed in the analyzed sample. An abundance of spores are present, and/or growth structures including hyphae and/or fruiting bodies are present and associated with one or more of the types of mold/fungi identified in the analyzed sample. NORMAL means that no presence of current or former growth was observed in the analyzed sample. If spores are recorded they are normally what is in the air and have settled on the surface(s) tested. Page 3 of 17
0 Prepared for : ACE ENVIRONMENTAL Test Address : PINNAMENENI 11121 BRIDGE HOUSE DR WINDERMERE, FL 34786 ANALYSIS METHOD Spore trap analysis Spore trap analysis Spore trap analysis Spore trap analysis LOCATION ENTERTAINMENT RM MASTER BEDROOM LAUNDRY ROOM FOYER-WALL CAVITY COC / LINE # 874317-9 874317-10 874317-11 874317-12 SAMPLE TYPE & VOLUME Z5-25L Z5-25L Z5-25L Z5-10L SERIAL NUMBER Q284173 Q284179 Q268146 Q284183 COLLECTION DATE Aug 9, 2015 Aug 9, 2015 Aug 9, 2015 Aug 9, 2015 ANALYSIS DATE Aug 12, 2015 Aug 12, 2015 Aug 12, 2015 Aug 12, 2015 CONCLUSION ELEVATED NOT ELEVATED ELEVATED ELEVATED IDENTIFICATION Aspergillus Bipolaris/Drechslera 1 2 Cercospora Chaetomium Cladosporium 6 2 5 Curvularia 1 2 1 100 2 Ganoderma Hyphae Nigrospora 1 2 1 1 3 2 4 160 20 1 2 4 0 10 Penicillium/Aspergillus 115 4,600 92 16 6 80 52 2,100 93 34 3,0 83 Pithomyces Pyricularia Smuts, myxomycetes 1 100 2 Unidentified 1 100 2 TOTAL SPORES 125 5,000 100 20 800 100 56 2,260 100 41 4,100 100 MINIMUM DETECTION LIMIT * 1 1 1 1 100 BACKGROUND DEBRIS Light Light Light too heavy for accurate count. Cellulose Fiber 1 3 2 80 Fiberglass 1 Insect Fragments Plant Fragments 1 1 1 Pollen OBSERVATIONS & COMMENTS s are estimated. Actual numbers of spores probably much higher. Background debris qualitatively estimates the amount of particles that are not pollen or spores and directly affects the accuracy of the spore counts. The categories of Light, Moderate, Heavy and Too Heavy for Accurate, are used to indicate the amount of deposited debris. Increasing amounts of debris will obscure small spores and can prevent spores from impacting onto the slide. The actual number of spores present in the sample is likely higher than reported if the debris estimate is Heavy or Too Heavy for Accurate. All calculations are rounded to two significant figures and therefore, the total percentage of spore numbers may not equal 100%. * Minimum Detection Limit. Based on the volume of air sampled, this is the lowest number of spores that can be detected and is an estimate of the lowest concentration of spores that can be read in the sample. NA = Not Applicable. that were observed from the samples submitted are listed on this report. If a spore is not listed on this report it was not observed in the samples submitted. Interpretation Guidelines: A determination is added to the report to help users interpret the mold analysis results. A mold report is only one aspect of an indoor air quality investigation. The most important aspect of mold growth in a living space is the availability of water. Without a source of water, mold generally will not become a problem in buildings. These determinations are in no way meant to imply any health outcomes or financial decisions based solely on this report. For questions relating to medical conditions you should consult an occupational or environmental health physician or professional. CONTROL is a baseline sample showing what the spore count and diversity is at the time of sampling. The control sample(s) is usually collected outside of the structure being tested and used to determine if this sample(s) is similar in diversity and abundance to the inside sample(s). ELEVATED means that the amount and/or diversity of spores, as compared to the control sample(s), and other samples in our database, are higher than expected. This can indicate that fungi have grown because of a water leak or water intrusion. Fungi that are considered to be indicators of water damage include, but are not limited to: Chaetomium, Fusarium, Memnoniella, Stachybotrys, Scopulariopsis, Ulocladium. NOT ELEVATED means that the amount and/or the diversity of spores, as compared to the control sample and other samples in our database, are lower than expected and may indicate no problematic fungal growth. UNUSUAL means that the presence of current or former growth was observed in the analyzed sample. An abundance of spores are present, and/or growth structures including hyphae and/or fruiting bodies are present and associated with one or more of the types of mold/fungi identified in the analyzed sample. NORMAL means that no presence of current or former growth was observed in the analyzed sample. If spores are recorded they are normally what is in the air and have settled on the surface(s) tested. Page 4 of 17
0 Prepared for : ACE ENVIRONMENTAL Test Address : PINNAMENENI 11121 BRIDGE HOUSE DR WINDERMERE, FL 34786 ANALYSIS METHOD Spore trap analysis Spore trap analysis INTENTIONALLY BLANK INTENTIONALLY BLANK LOCATION OUTSIDE FRONT OUTSIDE REAR COC / LINE # 874317-13 874317-14 SAMPLE TYPE & VOLUME Z5-25L Z5-25L SERIAL NUMBER Q284176 Q284249 COLLECTION DATE Aug 9, 2015 Aug 9, 2015 ANALYSIS DATE Aug 12, 2015 Aug 12, 2015 CONCLUSION CONTROL CONTROL IDENTIFICATION Aspergillus Bipolaris/Drechslera 1 2 Cercospora 1 2 Chaetomium Cladosporium 4 160 6 13 21 Curvularia 2 80 3 6 2 10 Ganoderma 6 2 10 5 8 Hyphae Nigrospora 2 80 3 9 360 14 3 5 33 1,300 52 23 37 Penicillium/Aspergillus 1 2 3 5 Pithomyces 1 2 Pyricularia 1 2 Smuts, myxomycetes 1 2 Unidentified 4 160 6 6 2 10 TOTAL SPORES 63 2,500 100 62 2,480 100 MINIMUM DETECTION LIMIT * 1 1 BACKGROUND DEBRIS Light Light Cellulose Fiber Fiberglass Insect Fragments Plant Fragments 1 1 Pollen 1 OBSERVATIONS & COMMENTS Background debris qualitatively estimates the amount of particles that are not pollen or spores and directly affects the accuracy of the spore counts. The categories of Light, Moderate, Heavy and Too Heavy for Accurate, are used to indicate the amount of deposited debris. Increasing amounts of debris will obscure small spores and can prevent spores from impacting onto the slide. The actual number of spores present in the sample is likely higher than reported if the debris estimate is Heavy or Too Heavy for Accurate. All calculations are rounded to two significant figures and therefore, the total percentage of spore numbers may not equal 100%. * Minimum Detection Limit. Based on the volume of air sampled, this is the lowest number of spores that can be detected and is an estimate of the lowest concentration of spores that can be read in the sample. NA = Not Applicable. that were observed from the samples submitted are listed on this report. If a spore is not listed on this report it was not observed in the samples submitted. Interpretation Guidelines: A determination is added to the report to help users interpret the mold analysis results. A mold report is only one aspect of an indoor air quality investigation. The most important aspect of mold growth in a living space is the availability of water. Without a source of water, mold generally will not become a problem in buildings. These determinations are in no way meant to imply any health outcomes or financial decisions based solely on this report. For questions relating to medical conditions you should consult an occupational or environmental health physician or professional. CONTROL is a baseline sample showing what the spore count and diversity is at the time of sampling. The control sample(s) is usually collected outside of the structure being tested and used to determine if this sample(s) is similar in diversity and abundance to the inside sample(s). ELEVATED means that the amount and/or diversity of spores, as compared to the control sample(s), and other samples in our database, are higher than expected. This can indicate that fungi have grown because of a water leak or water intrusion. Fungi that are considered to be indicators of water damage include, but are not limited to: Chaetomium, Fusarium, Memnoniella, Stachybotrys, Scopulariopsis, Ulocladium. NOT ELEVATED means that the amount and/or the diversity of spores, as compared to the control sample and other samples in our database, are lower than expected and may indicate no problematic fungal growth. UNUSUAL means that the presence of current or former growth was observed in the analyzed sample. An abundance of spores are present, and/or growth structures including hyphae and/or fruiting bodies are present and associated with one or more of the types of mold/fungi identified in the analyzed sample. NORMAL means that no presence of current or former growth was observed in the analyzed sample. If spores are recorded they are normally what is in the air and have settled on the surface(s) tested. Page 5 of 17
Master Bath Unidentified 2 2 2 1 10 100 1000 10000 per cubic meter Page 6 of 17
Living Room Unidentified 2 90 2 1 10 100 1000 10000 per cubic meter Page 7 of 17
Study Unidentified 2 23000 2 1 10 100 1000 10000 100000 per cubic meter Page 8 of 17
Entryway/foyer Unidentified 2 5600 2 1 10 100 1000 10000 per cubic meter Page 9 of 17
Hall Bath / Powder Rm Unidentified 2 4900 2 1 10 100 1000 10000 per cubic meter Page 10 of 17
Hall / Ah Closet #1 & 2 Unidentified 2 5800 2 1 10 100 1000 10000 per cubic meter Page 11 of 17
Entertainment Rm Unidentified 2 4600 2 2 1 10 100 1000 10000 per cubic meter Page 12 of 17
Master Bedroom Unidentified 2 6 160 2 1 10 100 1000 per cubic meter Page 13 of 17
Laundry Room Unidentified 2 2100 Nigros pora 2 Bipolaris /Drec hs lera 1 10 100 1000 10000 per cubic meter Page 14 of 17
Foyer-wall Cavity per cubic meter Page 15 of 17
Identification Outdoor Habitat Indoor Habitat Possible Allergic Potential Not an opinion or interpretation Common on wherever humidity is Known allergen causing Type I (hay Aspergillus too high. Grows on wallboard, fever and asthma) allergies and Common everywhere. Grows on leather, food, wood, etc. Capable Type III (hypersensitivity soil, dead plant material, nearly of growing over a wide range of pneumonitis). Can cause allergenic anything organic. moisture conditions from very dry sinusitis, and ABPA (allergenic to very wet. bronchopulmonar aspergillosis). Bipolaris/Drechslera Cercospora Chaetomium Cladosporium Curvularia Ganoderma Common everywhere. Frequently associated with grasses, but also found on plant material, decaying food, and soil. Common everywhere, especially growing on leaves. Growing on dung, dead leaves, wood. The most common spore type reported in the air worldwide. Found on dead and dying plant litter, and soil. Commonly found everywhere on soil and plant debris. Common everywhere growing on hardwood trees. Not known to grow indoors. None known. Cellulose substrates, especially wallboard, cardboard and wood. Not normally seen growing indoors unless the building material has been wetted. Unusual / Not Normal to be growing indoors. Commonly found on wood and wallboard. Commonly grows on window sills, textiles and foods. Capable of growing on many cellulytic substrates like wallboard and wood. None known. None known. Hyphae Common everywhere. All substrates. None known. Nigrospora Commonly found everywhere. Grows on decaying plant material Does not normally grow on building materials, but occasionally can be found growing on wallboard. Common Type I (hay fever and asthma), fungal sinusitis. Type I (hay fever and asthma) allergies. Type I (hay fever and asthma), Type III (hypersensitivity pneumonitis) allergies. Type I (hay fever and asthma) and common cause of allergenic sinusitis. Type I (hay fever and asthma) allergies. Comments Aspergillus fumigatus and Aspergillus niger are the most common species found in indoor air. This is a group of like-looking spores that include Bipolaris, Drechslera, Exserohilum, and sometimes Helminosporium. They cannot be consistently separated by spore morphology and are thus grouped together. Must be cultured to consistly separate the genera. Chaetomium is a water-indicating mold. of this type of mold should not be observed in significantly higher numbers in the air above background/control. If growth and/or significantly higher than backgroud/control spore numbers are reported, corrective action should be considered to reduce the source of water, moisture levels and/or spore numbers in the living space. A very common and important allergen source both outdoors and indoors. Hyphae are the "root-like" food absorption strands common to nearly all fungi. They sometimes can become airborne. Very distinctive spore that is easy to identify. Page 16 of 17
Identification Outdoor Habitat Indoor Habitat Possible Allergic Potential Not an opinion or interpretation Common everywhere. Constitutes a large part of the Ascospores airspora outside. Can reach Very few of this group grow Little known for most of this group of very high numbers in the air inside. The notable exception is fungi. Dependent on the type (see outside during the spring and Chaetomium, Ascotricha and Chaetomium and Ascotricha). summer. Can increase in Peziza. numbers during and after rainfalls. Basidiospores Penicillium/Aspergillus Pithomyces Pyricularia Smuts, myxomycetes Unidentified Commonly found everywhere, especially in the late summer and fall. These spores are from Mushrooms. Common everywhere. Normally found in the air in small amounts in outdoor air. Grows on nearly everything. Commonly seen everywhere growing dead leaves, soil and grasses. Common everywhere. Grows on grass leaves. Commonly found everywhere, espcially on logs, grasses and weeds. Common everywhere. Grow on decaying plant litter and other plant-derived material. Mushrooms are not normally found growing indoors, but can grow on wet lumber, especially in crawlspaces. Sometimes mushrooms can be seen growing in flower pots indoors. Wetted wallboard, wood, food, leather, etc. Able to grow on many substrates indoors. Not normally found growing indoors, sometimes on wallboard. Some allergenicity reported. Type I (hay fever, asthma) and Type III (hypersensitivity pneumonitis). Type I (hay fever and asthma) allergies and Type III (hypersensitivity pneumonitis) allergies. None known. Not known to grow indoors. None known. Smuts don`t normally grow indoors, but can occasionally be found on things brought from outside and stored in the house. Myxomycetes can occasionally grow indoors, but need lots of water to be established. Wetted cellulosic material. Type I (hay fever and asthma) allergies. None known. Comments Among the group of Mushrooms (Basidiomycetes) are dry rot fungi Serpula and Poria that are particularly destructive to buildings. This is a combination group of Penicillium and Aspergillus and is used when only the spores are seen. The spores are so similar that they cannot be reliably separated into their respective genera. Smuts and myxomycetes are a combined group of organisms because their spores look so similar and cannot be reliably distinquished from each other. This group of spores is reserved for spores whose identity is unknown. These kinds of spores have usually never been seen before in spore traps by our laboratory and/or are of such morphology that they cannot be identified with any degree of certainty to a particular genus. Page 17 of 17