Hydrolysis Unit B-4 / E-46 Technical data sheet The Hydrolysis Unit B-4 and E-46 ensure safe and fast acid hydrolysis for total fat content determination handling four or six samples simultaneously. The method complies with the standardized methods (Weibull Stoldt, AOAC). The B-4 (4 position) works with the B-8 extraction unit while the E-46 (6 position) is compatible with the E-86 extraction unit. POWER PREHEATING OFF HYDROLYSIS HEATING BÜCHI Hydrolysis Unit E-46
Scope of delivery All models are delivered ready to use and are packaged with: Components E-46 B-4 Sample tubes 8 4 Sample aspiration tubes 6 4 Rubber coupling, EPDM 6 4 Rubber coupling, Viton 4 Suction tube for E-46 Suction tube for B-4 Cap for rinsing sample tube 6 4 Stoppers, blind plug 8 4 Bottle of.5 kg quartz sand Order code Choose the configuration according to your needs: 0 4 8 7 Hydrolysis Unit E-46: Supply voltage 0 0 V ±0 % 50/60 Hz 0V ±0 % 50/60 Hz 0 4 4 4 6 Hydrolysis Unit E-46: Sealing modification 8 0 V ±0 % 50/60 Hz / Viton 0 7 4 Hydrolysis Unit B-4: Supply voltage, sealing material 5 5 0 V ±0 % 50/60 Hz, EPDM 5 6 0 V ±0 % 50/60 Hz, EPDM 6 0 V ±0 % 50/60 Hz, Viton Dimensions and weight Dimensions (WxHxD) Weight E-46 75 x 600 x 570 mm 4.5 kg B-4 75 x 600 x 570 mm.5 kg
Technical data B-4 E-46 Volume of glass sample tubes 0 ml 5 ml Height of glass sample tubes 7 ml 05 ml Volume of sample tubes Maximum sample weight Recommended particle size of quartz sand 00 ml 0g 0. - 0.9 mm Recommended diatomaceous earth Celite 55 Recommended concentration of hydrochloric acid 4 mol/l Mains voltage 0 V ± 0 % or 0 V ± 0 % Frequency Power consumption Overvoltage category 50/60 Hz 00W II Pollution degree Ambient conditions For indoor use only, altitude up to 000 m above sea level, maximum relative humidity 80 % for temperatures up to 0 C, ambient temperature 0-40 C Accessories Glass parts Qty Order number Picture Sample tubes 4 0777 Glass sample tubes with frit for B-8 4 078 Glass sample tubes with frit for E-8/86 04940 Glass sample tubes with frit for E-8/86 05578 Suction tube for B-4 0787 Suction tube for E-46 04868 Sample aspiration tube 0780 4 Additional parts Qty Order number Picture Rubber coupling, EPMD 078 5 Rubber coupling, Viton 4 0444 5 Rubber coupling, Viton 6 04449 5
Additional parts Qty Order number Picture Stoppers ø 45 mm, blind plug 4 0775 6 Cap for sample tube 4 0746 7 Upper insulation plate B-4 0746 Upper insulation plate E-46 0676 8 Pair of glass tongs 0004 Holder for sample tubes (6 pos.) 0409 Holder for sample tubes ( pos.) 0404 Holder for glass sample tubes, PTFE, microwavable (6 pos.) 0590 Holder for glass sample tubes, PP (4 pos.) 0746 Water jet pump, plastic 09 Vacuum hose Ø 0/0 0045 Quartz sand,.5 kg Special sand to be used for extraction and hydrolysis, fat free, fire dried, 0. - 0.9 mm Quartz sand, 5 kg Special sand to be used for extraction and hydrolysis, fat free, fire dried, 0. - 0.9 mm 07689 0495 IQ/OQ package for B-4/E-46 045659 Repeating OQ for B-4/E-46 05506 Accessories pictures 4 7 5 8 5 6 4
Functional principle For the Weibull-Stoldt fat determination in food and animal feed, the sample must be hydrolyzed to make the fat accessible for extraction, as solvents are not capable of breaking lipids bound as lipoproteins, liposaccharides or sterol esters. Therefore hydrolysis is used to transform these lipids into extractable forms, allowing reproducible fat determination. The sample is boiled in dilute hydrochloric acid (typically 4 mol/l) to break down proteins and high molecular weight carbohydrates into acid soluble constituents. The powerful IR-heating enables fast heat transfer to the sample vessels. The hydrolysis normally takes 0 min. Then the sample is filtered over a glass sample tube filled with sand and celite. The filter residue containing the fat is rinsed with water to become neutralized. Finally the filter residue is dried ready for extraction. The celite distributes the hydrolysate evenly in the tube, preventing any clumping and helps facilitate the extraction process. The glass sample tube is designed to be used directly in the Extraction units E-8/86 or B-8 without manual transfer of the sample. www.buchi.com/extraction Quality in your hands B-4 E-46 Data Sheet en A / Technical data are subject to change without notice / Quality Systems ISO 900 The English version is the original language version and serves as basis for all translations into other languages.