ab Colon cancer tissue array with progressive changes, 48 cases, 96 samples (1.5mm), set 1

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ab178131 Colon cancer tissue array with progressive changes, 48 cases, 96 samples (1.5mm), set 1 Instructions for Use Designed for IHC or ISH based protein or RNA tissue profiling in evolution and progression of cancers. This product is for research use only and is not intended for diagnostic use. Version: 2 Last Updated: 2 August 2016

Product Overview: ab178131 is a cancer tissue array, set 1, non-overlapping with ab178132, 96 cores, 48 cases from normal, premalignant and cancer tissues with progressive grades and TNM stages in duplicates. Case: 48 Core: 96 Format: 8 rows x 12 columns Size: 1.5mm Thickness: 4µm All the tissues were fixed in 10% neutral buffered formalin for 24 hours and processed using identical SOPs. Sections were picked onto Superfrost Plus or Startfrost Adhesive slides. There may be >5% tissue core losses for a slide. But retention rate should be >90%. Extra cores not shown on the array map are orientation markers and have no data. Notes for use: Bake the slides at 60 C for 30 minutes before use. If antigen retrieval is needed, it is always a good idea to start with a protocol with weak to mild strength. For hints and tips on Immunohistochemistry as well as other techniques please visit www.abcam.com/protocols. Keep up to date on Abcam events and conferences on Facebook & Twitter. 1

A01 30 F A02 28 M A03 64 M A04 55 M A05 71 M A06 54 M A07 5 M A08 52 M A09 30 F A10 50 M A11 49 F A12 48 M B01 30 F B02 28 M B03 64 M B04 55 M B05 71 M B06 54 M B07 5 M B08 52 M B09 30 F B10 50 M B11 49 F Benign Juvenile polyp Benign Juvenile polyp 2

B12 48 M C01 66 F C02 62 M C03 68 F C04 60 F C05 76 F C06 69 M C07 70 F C08 70 F C09 71 F C10 55 F C11 58 F C12 51 F D01 66 F D02 62 M D03 68 F D04 60 F D05 76 F D06 69 M D07 70 F D08 70 F D09 71 F D10 55 F Benign Adenoma Adenocarcinoma I T1N0M0 Adenocarcinoma II T3N2M0 Adenocarcinoma I Adenocarcinoma I T3N0M0 Adenocarcinoma I T3N0M0 Adenocarcinoma I~II Adenocarcinoma I T3N0M0 Adenocarcinoma I T3N0M0 Adenocarcinoma I~II Adenocarcinoma I T2N1M0 Adenocarcinoma I T2N1M0 Adenocarcinoma I T3N2M0 Adenocarcinoma I T1N0M0 Adenocarcinoma II T3N2M0 Adenocarcinoma I Adenocarcinoma I T3N0M0 Adenocarcinoma I T3N0M0 Adenocarcinoma I~II Adenocarcinoma I T3N0M0 Adenocarcinoma I T3N0M0 Adenocarcinoma I~II Adenocarcinoma I T2N1M0 3

D11 58 F D12 51 F E01 60 M E02 45 M E03 70 M E04 75 M E05 75 F E06 71 M E07 75 M E08 33 F E09 43 F E10 43 M E11 89 M E12 69 F F01 60 M F02 45 M F03 70 M F04 75 M F05 75 F F06 71 M F07 75 M F08 33 F F09 43 F Adenocarcinoma I T2N1M0 Adenocarcinoma I T3N2M0 Adenocarcinoma II Adenocarcinoma II Adenocarcinoma II T3N0M0 Adenocarcinoma II Adenocarcinoma II T3N0M0 Adenocarcinoma II T3N2M0 Adenocarcinoma II T3N0M0 Adenocarcinoma II Adenocarcinoma II T2N1M0 Adenocarcinoma II Adenocarcinoma II Adenocarcinoma II T3N0M0 Adenocarcinoma II Adenocarcinoma II Adenocarcinoma II T3N0M0 Adenocarcinoma II Adenocarcinoma II T3N0M0 Adenocarcinoma II T3N2M0 Adenocarcinoma II T3N0M0 Adenocarcinoma II Adenocarcinoma II T2N1M0 4

F10 43 M F11 89 M F12 69 F G01 72 F G02 66 M G03 72 F G04 41 F G05 42 M G06 52 M G07 46 F G08 55 M G09 37 M G10 56 M G11 60 M G12 48 M H01 72 F H02 66 M H03 72 F H04 41 F H05 42 M H06 52 M H07 46 F Adenocarcinoma II Adenocarcinoma II Adenocarcinoma II T3N0M0 Adenocarcinoma II T3N0M0 Adenocarcinoma III T3N1M1 Adenocarcinoma III Undifferentiated carcinoma Lymphoma, non- Hodgkin B cell lymphoma Lymphoma, non- Hodgkin lymphoma II Adenocarcinoma II T3N0M0 Adenocarcinoma III T3N1M1 Adenocarcinoma III Undifferentiated carcinoma 5

H08 55 M H09 37 M H10 56 M H11 60 M H12 48 M Lymphoma, non- Hodgkin B cell lymphoma Lymphoma, non- Hodgkin lymphoma II 6

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