Laser-Assisted In Situ Keratomileusis for Patients With Dry Eye

CLINICAL SCIENCES Laser-Assisted In Situ Keratomileusis for Patients With Dry Eye Ikuko Toda, MD; Naoko Asano-Kato, MD; Yoshiko Hori-Komai, MD; Kazuo Tsubota, MD Objective: To evaluate the efficacy and safety of laserassisted in situ keratomileusis (LASIK) in patients with preoperative dry eye. Methods: We divided the 543 eyes that underwent LASIK into the following 3 groups: eyes with definite dry eye ( group), with probable dry eye (P group), and without dry eye (N group). We evaluated visual outcome, dry-eye status, and complications. Outcome Measures: We compared uncorrected and best-corrected visual acuity, manifest refraction, symptoms, tear function, ocular surface abnormality, complications, corneal sensitivity, endothelial cell count, and patient satisfaction among the groups. Results: We found no significant differences among the groups in uncorrected and best-corrected visual acuity, manifest refraction, and patient satisfaction. A dry-eye symptom, dryness, was more severe in the than the N group after LASIK. The mean results of the Schirmer test with anesthesia and tear breakup times were significantly lower and the fluorescein score was higher in the than the N groups after LASIK. We found no differences in the incidence of complications among the groups. Corneal sensitivity was recovered within 6 months after LASIK in the and P groups and within 3 months in the N group. Conclusions: The efficacy and safety of LASIK were not affected by preexisting dry eye. However, preexisting dry eye is a risk factor for severe postoperative dry eye with lower tear function, more vital staining of the ocular surface, and more severe symptoms. Arch Ophthalmol. 22;12:124-128 From the Minamiaoyama Eye Clinic, Tokyo, Japan (Drs Toda, Asano-Kato, Hori-Komai, and Tsubota); and the Department of Ophthalmology, Tokyo Dental College, Chiba, Japan (Dr Tsubota). LASER-ASSISTED in situ keratomileusis (LASIK) is an effective procedure to correct refractive error, which induces little wound-healing response and inflammation in the corneal tissue. These factors contribute to stable postoperative refraction, excellent predictability, quick recovery of visual acuity, and no haze formation. However, some patients still experience regression, epithelial ingrowth, or postoperative inflammatory reactions such as diffuse lamellar and microbial keratitis. 1,2 In general, good candidates for LASIK should have no ocular and systemic diseases, including active uveitis, keratoconus, and diabetes mellitus, which may adversely affect postoperative wound healing. Although dry eye is not an absolute contraindication for LASIK, compromised tear function and ocular surface may influence the outcome of LASIK. Patients often request refractive surgery owing to contact lens intolerance caused by dry eye. A previous study from our group reported that photorefractive keratectomy could be performed safely on patients with contact lens intolerance due to dry eye as long as reflex tearing was noted. 3 Epithelial wound healing, recovery of corneal sensitivity, and visual outcomes of these patients were comparable with previously published data. It is expected that LASIK can be performed safely on patients with dry eye because less epithelial woundhealing response is involved postoperatively compared with photorefractive keratectomy. However, LASIK has its own disadvantages, ie, the corneal nerves are cut by making a flap and recovery time is increased 4 when dry eye itself develops. 5,6 Thus, we investigated whether the outcome, including visual performance and incidence of complications, was comparable between subjects with and without preoperative dry eye. Dry eye is a major reason for patients to consider LASIK, and it is a common post-lasik complication. 2,5,6 Our previous study demonstrated that post- LASIK dry eye develops with compro- 124

PATIENTS AND METHODS We enrolled 543 eyes of 29 consecutive patients (mean age, 33.1 years) who underwent LASIK from January 1, 1998, through March 31, 2, at the Minamiaoyama Eye Clinic, Tokyo, Japan, in this study. These eyes were divided into the following 3 groups on the basis of preoperative dry-eye status: eyes with definite dry eye ( group; 111 patients and 168 eyes), with probable dry eye (P group; 153 patients and 3 eyes), and without dry eye (N group; 49 patients and 75 eyes). Dry eye was diagnosed according to the modified criteria established by the Japanese Dry Eye Association. 8 The subcriteria consisted of results of a Schirmer test with anesthesia of no greater than 5 mm, and/or tear breakup time (BUT) of no greater than 5 seconds, and a fluorescein score of at least 1 and/or a rose bengal score of at least 3. Briefly, patients who satisfied both subcriteria were diagnosed as having ; those who satisfied 1 of 2 subcriteria, P. The mean±sd preoperative refraction in spherical equivalent was 7.24±3.9 diopters (D) in the group, 6.82±2.85 D in the P group, and 6.5±2.54 D in the N group. For the surgical procedure, a corneal flap was created with a microkeratome (LSK One; Moria, Antony, France; or MK-2; NIK Co, Ltd, Gamagori, Japan), and laser ablation was performed using an excimer laser (Apex-plus; Summit Technology, Walnut Creek, Calif; or EC-5; NIK Co, Ltd). Hyaluronate sodium (Hyalein; Santen Pharmaceutical Co, Ltd, Osaka, Japan) and balanced salt solution (BSS Plus; Alcon Laboratories, Ft Worth, Tex) were frequently applied intraoperatively to prevent dehydration of the ocular surface. Immediately after surgery, high viscous methylcellulose (Lacryvisc; Alcon, Hünenberg, Switzerland) was instilled in the eye and patients were asked to close their eyes and rest for 15 minutes. Low-dose corticosteroids (.1% fluorometholone [Flumetholon; Santen Pharmaceutical Co, Ltd]), antibiotics (ofloxacin [Tarivid; Santen Pharmaceutical Co, Ltd]), and.3% hyaluronate sodium eyedrops were prescribed 5 times a day. Eyedrop therapy was discontinued at 1 week postoperatively. Hyaluronate sodium eyedrops remain on the ocular surface longer than isotonic sodium chloride based artificial tears 9 and prevent the symptoms and oular surface damage caused by post-lasik dry eye. 7 To evaluate the efficacy of the LASIK correction, uncorrected (UCVA) and best-corrected visual acuity (BCVA) and manifest refraction in the spherical equivalent were examined at 1, 3, and 6 months and 1 year after LASIK. For the dry-eye examination, we assessed dry-eye symptoms and performed a Schirmer test with anesthesia, measurement of tear BUT, and fluorescein and rose bengal staining of the ocular surface. For subjective symptoms of dry eye, dryness was graded by the patients according to the following scale: indicates none; 1, mild; 2, moderate; 3, strong; and 4, very strong. The Schirmer test with anesthesia was performed 5 minutes after instilling 1 µl of.5% fluorescein sodium and.4% benoxinate hydrochloride into the conjunctival sac. The Schirmer strip was placed for another 5 minutes, and the length of the wet portion was measured. Vital staining of the ocular surface was performed by instillation of 2 µl of preservativefree mixed-dye solution (1% rose bengal and 1% fluorescein) into the conjunctival sac. 1 Fluorescein staining results were graded from to 3 for the upper, middle, and lower thirds of the cornea. Rose bengal staining results were graded from to 3 for the temporal conjunctiva, cornea, and nasal conjunctiva. Staining was graded by the extent as for negative; 1, scattered minute; 2, moderate spotty; and 3, diffuse blotchy. The tear BUT was measured as the number of seconds between the last complete blink and the first disturbance of the precorneal tear film. The safety of LASIK was evaluated by incidence of intraoperative (epithelial defect, bleeding, and flap repositioning) and postoperative (recurrent erosion, diffuse lamellar keratitis, microstriae, and epithelial ingrowth) complications, corneal sensitivity, loss of BCVA, and endothelial cell count. Corneal sensitivity was measured with an esthesiometer (Cochet-Bonnet; Luneau Ophthalmologie, Chartres, France) consisting of a nylon filament 6. mm long and.12 mm in diameter. Patients were asked to look straight ahead and to indicate when they believed that the top of the nylon filament touched the cornea. The measurement was started at 6. mm, and the length of the filament was decreased by 5.-mm increments to increase its rigidity. The corneal sensitivity was defined as the length of the filament that produced a first positive response. We photographed the corneal endothelium using a specular microscope (Konan Medical, Inc, Tokyo), and calculated mean cell density. We surveyed overall patient satisfaction with the outcome of LASIK, using a grade of 1 for very satisfied; 2, satisfied as expected; 3, not very satisfied; and 4, regretted undergoing LASIK. We performed statistical analysis by means of the t test, Wilcoxon rank sum test, or Kruskal-Wallis test. Values of P.5 were considered statistically significant. mised tear function and ocular surface for at least 1 month after surgery. 7 Tear function and dry-eye symptoms improve within several postoperative months in most cases; however, some patients still have dry eye 1 year after undergoing LASIK. Although the mechanisms for post- LASIK dry eye are unclear, more severe post-lasik dry eye may develop in patients with preoperative dry eye for longer periods compared with subjects without preoperative dry eye. The purpose of this study was to investigate whether (1) preoperative dry eye may affect the visual outcome and incidence of complications after LASIK, and (2) preoperative dry eye is a risk factor for severe postoperative dry eye. RESULTS VISUAL OUTCOME We found no significant differences among the, P, and N groups in UCVA and BCVA, except at 1 month, when the UCVA was better in the N than in the group (P=.3). Average postoperative manifest refraction was within ±.5 D in all groups at all follow-ups. Manifest refractive deviation due to emmetropia was slightly larger in the than in the N group at 3 months (.25±.76 vs.1±.55 D; P=.1), but we found no differences among the groups at other follow-up points. 125

4. 3.5 3. P N and within 3 months in the N group (Figure 4). However, no statistical difference was found among the groups at any follow-ups. Endothelial cell count was unchanged after LASIK in all groups. PATIENT SATISFACTION Dryness Score 2.5 We found no significant differences in satisfaction among the groups at 3 and 6 months and 1 year after surgery. 1. COMMENT.5. Figure 1. Dry-eye symptoms before and after laser-assisted in situ keratomileusis (LASIK) in the groups with definite () and probable dry eye (P) and without dry eye (N). Asterisk indicates P.1; dagger, P.5; and error bars, SD. DRY-EYE CONDITION We found significant differences between the and N groups in degree of symptoms of dryness before LASIK and at 3 and 6 months and 1 year after LASIK. Dryness was more severe in the P than in the N group at 6 months and 1 year after LASIK (Figure 1). The mean result of the Schirmer test with anesthesia was lower in the and P groups compared with the N group at all follow-ups after LASIK (Figure 2A). The tear BUT was shorter in the than in the N groups before LASIK and at all the follow-ups after LASIK, and the P group value was between those in the other groups (Figure 2B). For ocular surface abnormality, the fluorescein score was higher in the than in the N group before LASIK and at all follow-ups after LASIK, and staining intensity was between these values in the P group (Figure 3A). The rose bengal score was higher in the and P groups than in the N group preoperatively and at 3 months after LASIK (Figure 3B). SAFETY Intraoperative epithelial defect occurred in 4 eyes in the N group (P=.2). Fifty-eight eyes (34.5%) in the, 78 (26.%) in the P, and 2 (26.7%) in the N groups had bleeding from the pannus (P =.5). Flap repositioning was performed immediately after surgery owing to flap folds or dislocated flaps in 4 eyes (2.4%) in the, 11 (3.7%) in the P, and 2 (2.7%) in the N groups (P=.7). We found no significant differences in the incidence of postoperative complications, ie, epithelial ingrowth of 6 eyes (%) in the P group, diffuse lamellar keratitis of 1 eye (.6%) in the group, microstriae of 5 eyes (3.%) in the and 8 eyes (2.7%) in P groups, and no recurrent corneal erosion. Loss of BCVA of more than 2 lines was observed in 2 eyes (1.2%) in the, 7 (2.3%) in the P, and 1 (1.3%) in the N groups (P=.4) at 1 year after LASIK. Corneal sensitivity recovered to preoperative levels within 6 months after LASIK in the and P groups Dry eye is a common disease that often causes patients to consider LASIK because of difficulty wearing contact lenses. Among all patients who underwent LASIK in our clinic before March 31, 2, 35.2% and 41.2% of patients were diagnosed as having and P, respectively, according to the modified criteria of the Japanese Dry Eye Association. 8 Thus, more than 75% of the patients undergoing LASIK have preoperative dry eye. In the present report, we compared the outcome of LASIK among patients who had, P, and N. The efficacy of LASIK, determined by means of postoperative UCVA, BCVA, and manifest refraction, was almost comparable in the 3 groups. However, UCVA at 1 month and manifest refraction at 3 months were better in the N than in the group. We speculate that these differences were due to slight differences in the amount of correction between the groups. The mean refractive correction was 7.2 D in the group and 6.47 D in the N group (P=.7). Tear function and ocular surface condition determined by results of the Schirmer test, tear BUT, and rose bengal and fluorescein stainings were more compromised after LASIK in the group compared with the N group. This finding suggests that patients with preoperative dry eye have more severe post-lasik dry eye compared with the N group. The examination data of patients in the P group yielded values that were between those of the and N groups. A typical dryeye complaint, dryness, was more marked in the group; however, the differences among all groups were not significant at 1 month after LASIK, because this symptom temporarily increased in the N group and returned to preoperative levels by the follow-up. In contrast, the subjective score of dryness was more severe and unchanged in the group throughout the follow-up. These data may indicate that preoperative dry eye is a risk factor for severe postoperative dry eye. The precise mechanism for post-lasik dry eye is not clear. Wilson 11 suggested the term LASIK-induced neurotrophic epitheliopathy (LNE) for a condition of transient ocular surface abnormality, such as punctate keratitis and rose bengal staining of the cornea after LASIK. They speculate that LNE is attributable to interruption of sensory nerve input to the corneal epithelium, which may last for 6 months or longer postoperatively. They also suggest that preexisting dry eye may be a risk factor for LNE. Although tear function is not affected in this condition, LNE and post-lasik dry eye may be caused by the same mechanism of neurotrophic damage. Our results in this study indicate that preexisting dry eye could 126

A 3 B 14 P N Schirmer Value, mm 25 2 15 1 Tear BUT, s 12 1 8 6 4 5 2 Figure 2. Tear function before and after laser-assisted in situ keratomileusis (LASIK) in the, P, and N groups. A, Schirmer value. B, Tear breakup time (BUT). Asterisk indicates P.1; dagger, P.5; and error bars, SD. Group names are explained in the legend to Figure 1. A Fluorescein Score 4. 3.5 3. 2.5 B Rose Bengal Score 1. P N 1..5.5.. Figure 3. Vital staining of ocular surface before and after laser-assisted in situ keratomileusis (LASIK) in the, P, and N groups. A, Fluorescein score. B, Rose bengal score. Asterisk indicates P.1; dagger, P.5; and error bars, SD. Group names are explained in the legend to Figure 1. lead to postoperative uncomfortable symptoms caused by dry eye and/or LNE. Epithelial problems during or after LASIK surgery were of biggest concern in patients with preexisting dry eye. Although we treated some patients who had severe ocular surface damage caused by dry eye with punctal plugs before surgery, most of our patients underwent LASIK without any dry-eye pretreatment except for artificial tears. Epithelial defect, recurrent erosion, and epithelial ingrowth did not preferentially occur in patients with preoperative dry eye. Incidence of other problems, such as flap dislocation, flap folds, microstriae, and diffuse lamellar keratitis was not increased in patients with dry eye. For long-term complications, we found no statistically singnificant difference in the loss of BCVA between the groups. However, recovery of corneal sensitivity was significantly slower in patients with dry eye. This finding may be explained by dysfunction of tear dynamics in dry eye, because corneal sensitivity is sometimes decreased in these patients. 12 Alternatively, delayed recovery of corneal nerves may be responsible for tear deficiency in these patients. The other possibility is that the difference may be attributable to the amount of corneal tissue ablation, which was increased in the Corneal Sensitivity, cm 8 7 6 5 Kruskal- Wallis Test Preoperative P =.34 1 mo P =.13 3 mo P =.14 6 mo P =.21 P N 1 y P =.27 Figure 4. Corneal sensitivity before and after laser-assisted in situ keratomileusis (LASIK) in the, P, and N groups. Asterisk indicates P.1; dagger, P.5; and error bars, SD. The P values for the differences between the groups at the same follow-ups are shown at the bottom. Group names are explained in the legend to Figure 1. group in this study. Kim and Kim 13 reported that greater reduction of corneal sensitivity was observed in cases with deeper ablation. 127

CONCLUSIONS Our results indicate that the efficacy and safety of LASIK were not affected by preexisting dry-eye status. With the proper ocular surface management, patients with dry eye can be good candidates for LASIK. However, our results also suggest that preexisting dry eye is a risk factor for severe postoperative dry eye with lower tear function, more vital staining of the ocular surface, and more severe dry-eye symptoms until 1 year after LASIK. Patients with dry eye who expect complete resolution of their symptoms after LASIK with removal of contact lenses should be warned that their dry-eye symptoms may persist after LASIK. We have recently found that some patients complain of dry-eye symptoms for more than 1 year after LASIK. The pathogenesis and risk factors of such symptoms are now under investigation. Submitted for publication October 23, 21; final revision received March 19, 22; accepted April 16, 22. We thank Chikako Sakai of the Minamiaoyama Eye Clinic for her assistance in statistical analyses. Corresponding author and reprints: Ikuko Toda, MD, Minamiaoyama Eye Clinic, 2-27-25 Minamiaoyama, Minatoku, Tokyo 17-62, Japan (e-mail: ikuko@tka.att.ne.jp). REFERENCES 1. Alio JL, Perez-Santonja JJ, Tervo T, et al. Postoperative inflammation, microbial complications, and wound healing following laser in situ keratitis. J Refract Surg. 2;16:523-528. 2. Ambrosio RJ, Wilson S. Complications of laser in situ keratomileusis: etiology, prevention, and treatment. J Refract Surg. 21;17:35-379. 3. Toda I, Yagi Y, Hata S, Itoh S, Tsubota K. Excimer laser photorefractive keratectomy for patients with contact lens intolerance caused by dry eye. Br J Ophthalmol. 1996;8:64-69. 4. Linna T, Perez-Santonja J, Terve K, Sakla H, Alioy y Sanz, J, Tervo T. Recovery of corneal nerve morphology following laser in situ keratomileusis. Exp Eye Res. 1998;66:755-763. 5. Hong J, Kim H. The changes of tear break up time after myopic excimer laser photorefractive keratectomy. Korean J Ophthalmol. 1997;11:89-93. 6. Aras C, Ozdamar A, Bahcecioglu H, Karacorlu M, Sener B, Ozkan S. Decreased tear secretion after laser in situ keratomileusis for high myopia. J Refract Surg. 2;16:362-364. 7. Toda I, Kato-Asano N, Hori-Komai Y, Tsubota K. Dry eye after laser in situ keratomileusis. Am J Ophthalmol. 21;132:1-7. 8. Shimazaki J. Definition and criteria of dry eye. Ganka. 1995;37:765-77. 9. Shimmura S, Ono M, Shinozaki K, et al. Sodium hyaluronate eyedrops in the treatment of dry eye. Br J Ophthalmol. 1995;79:17-111. 1. Toda I, Tsubota K. Practical double vital staining for ocular surface evaluation [letter]. Cornea. 1993;12:366-367. 11. Wilson S. Laser in situ keratomileusis induced (presumed) neurotrophic epitheliopathy. Ophthalmology. 21;18:182-187. 12. Xu KP, Yagi Y, Tsubota K. Decrease in corneal sensitivity and change in tear function in dry eye. Cornea. 1996;15:235-239. 13. Kim W, Kim J. Change in corneal sensitivity following laser in situ keratomileusis. J Cataract Refract Surg. 1999;25:368-373. Notice to the Authors of Reports From Clinical Trials T he Journal of the American Medical Association ( JAMA) and the Archives of Ophthalmology function as an editorial consortium. With one submission and one set of reviews, your clinical trial manuscript will be considered for publication in both JAMA and the Archives of Ophthalmology. Submit your paper to the journal of your choice according to the appropriate Instructions for Authors and the following guidelines will apply: 1. If your manuscript is accepted by JAMA, it will be considered for an editorial or commentary in JAMA. Your abstract will also be published in the Archives of Ophthalmology with a commentary or editorial. 2. If your manuscript is accepted by the Archives of Ophthalmology, it will be considered for an editorial or commentary in the Archives of Ophthalmology. Your abstract will also be considered for publication in JAMA. 128