Analytical and Preparative SFC Columns

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Transcription:

Analytical and Preparative SFC Columns Best Performance for Supercritical Fluid Chromatography Sepax SFC-Cyano... Sepax SFC-Amino... Sepax SFC-Pyridine... Sepax SFC-SCX... 5 Sepax SFC-Diol... 6 Sepax SFC-Silica... 7 Sepax Technologies, Inc. 5 Innovation Way, Newark, Delaware 97, USA Phone: () 66-; Fax: () 66-5 Toll Free: -877-Sepax-US www.sepax-tech.com

Sepax SFC-Cyano polar compounds. The monomeric bonding chemistry produces very high efficiency and high resolution separations. CH SFC test chromatogram SiO O Si (CH ) CH CN 7 6 5 Silica: Spherical, high purity (< ppm metals) Pore size: Å Particle size:, 5, 7 and m Pore volume:. ml/g Surface area: m /g Phase structure: Monomeric and fully endcapped % Carbon: 7.% Coverage: ~.5 mol/m mau (N=,) 5 6 7 8 9 LC test chromatogram.6.5. (N=,65) Sym=.7 Column: Sepax SFC-Cyano (5µ,.6x5 mm) Mobile phase: CO /MeOH Gradient: t (min) 6 7 8 9 MeOH(%) 5 5 5 5 Flow rate:. ml/min Detection (DAD): 5 nm Instrument: Berger Analytical SFC Sample:. Dimethylphthalate. Caffeine. Uracil. Cortisone AU.... 5 6 7 8 9 Column: SFC-Cyano (5µm, Å,.6x5mm) Mobile phase: 55% Acetonitrile/5% H O Flow rate:. ml/min Detector (UV): 5 nm Injection volume:. µl Temperature: Ambient ( o C) Sample:. -Cyanophenol. Anisole. Benzophenone The SFC-Cyano packing materials are bonded with propyl cyano functional groups that enable special interaction with Characteristics Highly controlled chemistry of monolayer formation and end-capping Extremely high column-to-column reproducibility High selectivity and efficiency for separations Suitable for separations of acidic, neutral and basic organic compounds, and pharmaceuticals Sepax SFC-Cyano Products Sepax SFC-Cyano Pore size P/N 5 m,.6x5mm Å SEP65-65 5 m,.6x5mm Å SEP65-65 5 m,.6x5mm Å SEP65-65 5 m,.x5mm Å SEP65-5 5 m,.x5mm Å SEP65-5 5 m,.x5mm Å SEP65-5 5 m, x5mm Å SEP65-5 5 m, x5mm Å SEP65-5 5 m, x5mm Å SEP65-5

Sepax SFC-Amino SiO O O Si O Silica: Spherical, high purity (< ppm metals) Pore size: Å Particle size:, 5, 7 and m Pore volume:. ml/g Surface area: m /g Phase structure: Polymeric and no endcapping % Carbon:.% Coverage: ~.6 mol/m LC test chromatogram (CH ) NH Characteristics Well controlled chemistry of polymeric monolayer High column-to-column reproducibility Utilized as both SFC and LC phases Versatile mobile phases: non-aqueous solvents, such as hexane/ethyl acetate and chloroform/methanol, and aqueous solutions Recommended for separations of pharmaceuticals, saccharides, nucleotides, and basic organic compounds Sepax SFC-Amino Products Sepax SFC-Amino Pore size P/N 5 m,.6x5mm Å SEP655-65 5 m,.6x5mm Å SEP655-65 5 m,.6x5mm Å SEP655-65 5 m,.x5mm Å SEP655-5 5 m,.x5mm Å SEP655-5 5 m,.xmm Å SEP655-5 m,.x5mm Å SEP655-5 5 m, x5mm Å SEP655-5 5 m, x5mm Å SEP655-5 5 m, xmm Å SEP655-5 m, x5mm Å SEP655-5.5. Peak N=, Sym=. AU.... 6 8 6 8 Column: SFC-Amino (5µm, Å,.6x5mm) Mobile phase:.7% EtOH/.% H O/85% Isooctane Flow rate:. ml/min Detector (UV): 5 nm Injection volume:. µl Temperature: Ambient ( o C) Sample:. Toluene. Benzanilide. p-nitroaniline

Sepax SFC-Pyridine SFC test chromatogram O SiO O Si CH CH O N Vendor X N(#)=, Vendor Y N(#)=8,86 Silica: Spherical, high purity (< ppm metals) Pore size: Å Particle size:, 5, 7, and m Pore volume:. ml/g Surface area: m /g Phase structure: Polymeric and no endcapping % Carbon: 8.% Coverage: ~. mol/m LC test chromatogram.7.6.5 Sepax N(#)=8, 5 6 7 8 Column: Pyridine phases (5µ,.6x5 mm) Mobile phase: CO /MeOH Gradient: t (min) 6 7 8 9 MeOH(%) 5 5 5 5 Flow rate:. ml/min Detection (DAD): nm Instrument: Berger Analytical SFC Sample:. Dimethylphthalate. Caffeine. Uracil. Cortisone 9. AU. 5... 6 8 6 8 Column: SFC-Pyridine (5µm, Å,.6x5mm) Mobile phase: 8% Hexane/% Ethanol Flow rate:. ml/min Detector (UV): 5 nm Injection volume:. µl Temperature: Ambient ( o C) Sample:. Pyridine. Aniline. Phenol. Uracil 5. p-aminobenzoic acid Characteristics Well controlled chemistry of polymeric monolayer High column-to-column reproducibility Recommended for separations of acidic, neutral and basic organic compounds, as well as the pharmaceuticals Sepax SFC-Pyridine Products SFC-Pyridine Pore size P/N 5 m,.6x5mm Å SEP6955-65 5 m,.6x5mm Å SEP6955-65 5 m,.6x5mm Å SEP6955-65 5 m,.x5mm Å SEP6955-5 5 m,.x5mm Å SEP6955-5 5 m,.xmm Å SEP6955-5 m, x5mm Å SEP6955-5 5 m, x5mm Å SEP6955-5 5 m, xmm Å SEP6955-

Sepax SFC-SCX Phase Structure SiO SO H Si Si Mixed-mode of ion-exchange and hydrophobic interaction enabling high selectivity and appropriate retention for a variety of compounds Polymeric bonding and end-capping to achieve the exceptionally high stability ph stability:.5-8. Suitable for separations of a complex of cationic, nitrogen containing, and neutral compounds LC test chromatogram Technical Specifications Silica: Spherical, high purity (< ppm metals) Pore size: Å Particle size:, 5 m Pore volume:. ml/g Surface area: m /g Phase structure: Polymeric and mixed mode % Carbon:.% Coverage:. mol/m Description Sepax SFC-SCX is a silica based mixed-mode strong cation exchange phase. Its phase has a mixed chemical structure of sulfonic acid and phenyl group. This mixed mode of strong cation-exchange and hydrophobicity offers high selectivity and high resolution separation for cationic/basic and nitrogen containing compounds as well as desirable retention for a variety of weak cationic and neutral organic compounds. A uniform, polymeric bonding of Sepax SFC-SCX enhances its stability under various separation conditions. mau 8 6 Column: Sepax SFC-SCX (.6x5 mm, 5 m) Mobile phase: 5 mm phosphate buffer, ph.5 Flow rate:.5 ml/min (8 bar) Detector: UV 8 nm Injection volume: 5 L Temperature: Ambient ( o C) Compounds:. Uridine (. mg/ml). Cytidine (. mg/ml). Guanosine (. mg/ml). Inosine and impurities (. mg/ml) 5. Adenosine (. mg/ml) 5 6 8 min Sepax SFC-SCX has expanded its applications from the traditional SCX separation of cationic and nitrogen containing compounds to weak cationic and neutral organic molecules. Examples include separation of analytes of amines and polyamines, such as alkaloids, peptides, codeine, cough and cold ingredients. Characteristics Highly controlled chemistry of polymeric monolayer formation and end-capping Mixed chemical structure of sulfonic acid and phenyl group Sepax SFC-SCX Products Sepax SFC-SCX Pore size P/N 5 m,.6x5mm Å SEP665-65 5 m,.6x5mm Å SEP665-65 5 m,.6x5mm Å SEP665-65 5 m,.x5mm Å SEP665-5 5 m,.x5mm Å SEP665-5 5 m,.xmm Å SEP665-5 m, x5mm Å SEP665-5 5 m, x5mm Å SEP665-5 5 m, xmm Å SEP665-5

Sepax SFC-Diol SFC test chromatogram 7 O SiO O Si CH CH CH SiO Si CH CH CH O OH O CH CHCH OH mau 6 5 5 nm (N=6,) Silica: Spherical, high purity (< ppm metals) Pore size: 8, Å Particle size:, 5, 7 and m Pore volume:. ml/g Surface area: m /g Phase structure: Monomeric and fully endcapped % Carbon: 8.8% Coverage: ~. mol/m LC test chromatogram.8.7.6 nm 5 6 7 Column: Sepax SFC-Diol (5µ,.6x5 mm) Mobile phase: CO /MeOH Gradient: t (min) 6 7 8 9 MeOH(%) 5 5 5 5 Flow rate:. ml/min Detection (DAD): 5 nm Instrument: Berger Analytical SFC Sample:. Dimethylphthalate. Caffeine. Uracil. Cortisone 8 9 AU.5..... 5 6 7 8 9 Column: SFC-Diol (5µm, Å,.6x5mm) Mobile phase: 8% Hexane/% Ethanol Flow rate:. ml/min Detector (UV): 5 nm Injection volume:. µl Temperature: Ambient ( o C) Sample:. Phenol. -Nitrophenol.,-Biphenol Characteristics Well controlled chemistry of polymeric monolayer High column-to-column reproducibility Polar phase for separations of acidic, neutral and basic organic compounds, as well as the pharmaceuticals High selectivity and efficiency for separations Sepax SFC-Diol Products Sepax SFC-Diol Pore size P/N 5 m,.6x5mm Å SEP665-65 5 m,.6x5mm Å SEP665-65 5 m,.6x5mm Å SEP665-65 5 m,.x5mm Å SEP665-5 5 m,.x5mm Å SEP665-5 5 m,.xmm Å SEP665-5 m, x5mm Å SEP665-5 5 m, x5mm Å SEP665-5 5 m, x5mm Å SEP665-5 6

Sepax SFC-Silica SiO O H Suitable for separations in aqueous and non-aqueous mobile phases Suitable for separations of polar and basic organic compounds, such as vitamins, steroids, as well as pharmaceuticals SFC test chromatogram, Silica: Spherical, high purity (< ppm metals) Pore size: Å Particle size:, 5, 7 and µm Pore volume:. ml/g Surface area: m /g Phase structure: activated hydroxyl (-OH) % Carbon: % LC test chromatogram,, 9 8 mau 7 6 5 (N=7,) AU.7.6.5... Peak N=,6 Sym=. 5 6 7 8 Column: Sepax SFC-Silica (5µ,.6x5 mm) Mobile phase: CO /MeOH Gradient: t (min) 6 7 8 9 MeOH(%) 5 5 5 5 Flow rate:. ml/min Detection (DAD): nm Instrument: Berger Analytical SFC Sample:. Dimethylphthalate. Caffeine. Uracil. Cortisone 9.. 6 8 6 8 Column: SFC-Silica (5µm, Å,.6x5mm) Mobile phase:.7% EtOH/.% H O/85% Isooctane Flow rate:. ml/min Detector (UV): 5 nm Injection volume:. µl Temperature: Ambient ( o C) Sample:. Toluene. Benzanilide. p-nitroaniline Characteristics Activated silica surface Ultra high purity Enhanced mechanical stability Sepax SFC-Diol Products Sepax SFC-Silica Pore size P/N 5µm,.6x5mm Å SEP675-65 5µm,.6x5mm Å SEP675-65 5µm,.6x5mm Å SEP675-65 5µm,.x5mm Å SEP675-5 5µm,.x5mm Å SEP675-5 5µm,.xmm Å SEP675-5µm, x5mm Å SEP675-5 5µm, x5mm Å SEP675-5 5µm, x5mm Å SEP675-5 7

SFC Analytical and Preparative Column Products Ordering Information: µm Analytical Columns (length x ID mm) x. 5x. x. 5x. 5x. x.* SFC-Cyano ( Å) SEP6- SEP6-5 SEP6- SEP6-5 SEP6-5 SEP6- SFC-NH ( Å) SEP65- SEP65-5 SEP65- SEP65-5 SEP65-5 SEP65- SFC-SCX ( Å) SEP66- SEP66-5 SEP66- SEP66-5 SEP66-5 SEP66- SFC-Pyridine (Å) SEP695- SEP695-5 SEP695- SEP695-5 SEP695-5 SEP695- SFC-Diol (8 Å) SEP65- SEP65-5 SEP65- SEP65-5 SEP65-5 SEP65- SFC-Diol ( Å) SEP66- SEP66-5 SEP66- SEP66-5 SEP66-5 SEP66- SFC-Silica ( Å) SEP67- SEP67-5 SEP67- SEP67-5 SEP67-5 SEP67- * cartridge does not include the cartridge holder µm Analytical Columns (length x ID mm) x.6 5x.6 x.6 5x.6 5x.6 x.* SFC-Cyano ( Å) SEP6-6 SEP6-65 SEP6-6 SEP6-65 SEP6-65 SEP6- SFC-NH ( Å) SEP65-6 SEP65-65 SEP65-6 SEP65-65 SEP65-65 SEP65- SFC-SCX ( Å) SEP66-6 SEP66-65 SEP66-6 SEP66-65 SEP66-65 SEP66- SFC-Pyridine (Å) SEP695-6 SEP695-65 SEP695-6 SEP695-65 SEP695-65 SEP695- SFC-Diol (8 Å) SEP65-6 SEP65-65 SEP65-6 SEP65-65 SEP65-65 SEP65- SFC-Diol ( Å) SEP66-6 SEP66-65 SEP66-6 SEP66-65 SEP66-65 SEP66- SFC-Silica ( Å) SEP67-6 SEP67-65 SEP67-6 SEP67-65 SEP67-65 SEP67- * cartridge does not include the cartridge holder 5 µm Analytical Columns (length x ID mm) x. 5x. x. 5x. 5x. x.* SFC-Cyano ( Å) SEP65- SEP65-5 SEP65- SEP65-5 SEP65-5 SEP65- SFC-NH ( Å) SEP655- SEP655-5 SEP655- SEP655-5 SEP655-5 SEP655- SFC-SCX ( Å) SEP665- SEP665-5 SEP665- SEP665-5 SEP665-5 SEP665- SFC-Pyridine (Å) SEP6955- SEP6955-5 SEP6955- SEP6955-5 SEP6955-5 SEP6955- SFC-Diol (8 Å) SEP655- SEP655-5 SEP655- SEP655-5 SEP655-5 SEP655- SFC-Diol ( Å) SEP665- SEP665-5 SEP665- SEP665-5 SEP665-5 SEP665- SFC-Silica ( Å) SEP675- SEP675-5 SEP675- SEP675-5 SEP675-5 SEP675- * cartridge does not include the cartridge holder 8

Ordering Information: 5 µm Analytical Columns (length x ID mm) x.6 5x.6 x.6 5x.6 5x.6 x.* SFC-Cyano ( Å) SEP65-6 SEP65-65 SEP65-6 SEP65-65 SEP65-65 SEP65- SFC-NH ( Å) SEP655-6 SEP655-65 SEP655-6 SEP655-65 SEP655-65 SEP655- SFC-SCX ( Å) SEP665-6 SEP665-65 SEP665-6 SEP665-65 SEP665-65 SEP665- SFC-Pyridine (Å) SEP6955-6 SEP6955-65 SEP6955-6 SEP6955-65 SEP6955-65 SEP6955- SFC-Diol (8 Å) SEP655-6 SEP655-65 SEP655-6 SEP655-65 SEP655-65 SEP655- SFC-Diol ( Å) SEP665-6 SEP665-65 SEP665-6 SEP665-65 SEP665-65 SEP665- SFC-Silica ( Å) SEP675-6 SEP675-65 SEP675-6 SEP675-65 SEP675-65 SEP675- * cartridge does not include the cartridge holder 5 µm Preparative and Semi-preparative Columns (length x ID mm) 5x7.8 5x7.8 5x 5x x.* SFC-Cyano ( Å) SEP65-785 SEP65-785 SEP65-5 SEP65-5 SEP65- SFC-NH( Å) SEP655-785 SEP655-785 SEP655-5 SEP655-5 SEP655- SFC-SCX ( Å) SEP665-785 SEP665-785 SEP665-5 SEP665-5 SEP665- SFC-Pyridine ( Å) SEP6955-785 SEP6955-785 SEP6955-5 SEP6955-5 SEP6955- SFC-Diol (8 Å) SEP655-785 SEP655-785 SEP655-5 SEP655-5 SEP655- SFC-Diol ( Å) SEP665-785 SEP665-785 SEP665-5 SEP665-5 SEP665- SFC-Silica ( Å) SEP675-785 SEP675-785 SEP675-5 SEP675-5 SEP675- * cartridge does not include the cartridge holder 5 µm Preparative and Semi-Preparative Columns (length x ID mm) 5x. x. 5x. 5x. x. x.** SFC-Cyano ( Å) SEP65-5 SEP65- SEP65-5 SEP65-5 SEP65- SEP65- SFC-NH ( Å) SEP655-5 SEP655- SEP655-5 SEP655-5 SEP655- SEP655- SFC-SCX ( Å) SEP665-5 SEP665- SEP665-5 SEP665-5 SEP665- SEP665- SFC-Pyridine (Å) SEP6955-5 SEP6955- SEP6955-5 SEP6955-5 SEP6955- SEP6955- SFC-Diol (8 Å) SEP655-5 SEP655- SEP655-5 SEP655-5 SEP655- SEP655- SFC-Diol ( Å) SEP665-5 SEP665- SEP665-5 SEP665-5 SEP665- SEP665- SFC-Silica ( Å) SEP675-5 SEP675- SEP675-5 SEP675-5 SEP675- SEP675- ** cartridge does not include the cartridge holder 9

Ordering Information: 5 µm Preparative and Semi-Preparative Columns (length x ID mm) 5x x 5x 5x 5x5 x.** SFC-Cyano ( Å) SEP65-5 SEP65- SEP65-5 SEP65-5 SEP65-55 SEP65- SFC-NH ( Å) SEP655-5 SEP655- SEP655-5 SEP655-5 SEP655-55 SEP655- SFC-SCX ( Å) SEP665-5 SEP665- SEP665-5 SEP665-5 SEP665-55 SEP665- SFC-Pyridine (Å) SEP6955-5 SEP6955- SEP6955-5 SEP6955-5 SEP6955-55 SEP6955- SFC-Diol (8 Å) SEP655-5 SEP655- SEP655-5 SEP655-5 SEP655-55 SEP655- SFC-Diol ( Å) SEP665-5 SEP665- SEP665-5 SEP665-5 SEP665-55 SEP665- SFC-Silica ( Å) SEP675-5 SEP675- SEP675-5 SEP675-5 SEP675-55 SEP675- ** cartridge does not include the cartridge holder µm Preparative and Semi-preparative Columns (length x ID mm) 5x7.8 x 5x 5x x.* SFC-Cyano ( Å) SEP69-785 SEP69- SEP69-5 SEP69-5 SEP69- SFC-NH( Å) SEP659-785 SEP659- SEP659-5 SEP659-5 SEP659- SFC-SCX ( Å) SEP669-785 SEP669- SEP669-5 SEP669-5 SEP669- SFC-Pyridine ( Å) SEP6959-785 SEP6959- SEP6959-5 SEP6959-5 SEP6959- SFC-Diol (8 Å) SEP659-785 SEP659- SEP659-5 SEP659-5 SEP659- SFC-Diol ( Å) SEP669-785 SEP669- SEP669-5 SEP669-5 SEP669- SFC-Silica ( Å) SEP679-785 SEP679- SEP679-5 SEP679-5 SEP679- * cartridge does not include the cartridge holder µm Preparative and Semi-preparative Columns (length x ID mm) 5x. x. 5x. 5x. x. x.** SFC-Cyano ( Å) SEP69-5 SEP69- SEP69-5 SEP69-5 SEP69- SEP69- SFC-NH ( Å) SEP659-5 SEP659- SEP659-5 SEP659-5 SEP659- SEP659- SFC-SCX ( Å) SEP669-5 SEP669- SEP669-5 SEP669-5 SEP669- SEP669- SFC-Pyridine (Å) SEP6959-5 SEP6959- SEP6959-5 SEP6959-5 SEP6959- SEP6959- SFC-Diol (8 Å) SEP659-5 SEP659- SEP659-5 SEP659-5 SEP659- SEP659- SFC-Diol ( Å) SEP669-5 SEP669- SEP669-5 SEP669-5 SEP669- SEP669- SFC-Silica ( Å) SEP679-5 SEP679- SEP679-5 SEP679-5 SEP679- SEP679- ** cartridge does not include the cartridge holder

Ordering Information: µm Preparative and Semi-preparative Columns (length x ID mm) 5x x 5x 5x 5x5 x.** SFC-Cyano ( Å) SEP69-5 SEP69- SEP69-5 SEP69-5 SEP69-55 SEP69- SFC-NH ( Å) SEP659-5 SEP659- SEP659-5 SEP659-5 SEP659-55 SEP659- SFC-SCX ( Å) SEP669-5 SEP669- SEP669-5 SEP669-5 SEP669-55 SEP669- SFC-Pyridine (Å) SEP6959-5 SEP6959- SEP6959-5 SEP6959-5 SEP6959-55 SEP6959- SFC-Diol (8 Å) SEP659-5 SEP659- SEP659-5 SEP659-5 SEP659-55 SEP659- SFC-Diol ( Å) SEP669-5 SEP669- SEP669-5 SEP669-5 SEP669-55 SEP669- SFC-Silica ( Å) SEP679-5 SEP679- SEP679-5 SEP679-5 SEP679-55 SEP679- ** cartridge does not include the cartridge holder ) * Cartridge holder P/N# SEP-, for x mm guard cartridge P/N# SEP-, for x mm guard cartridge ) ** Cartridge holder P/N# SEP-, for x. mm guard cartridge Cartridge Holder ) ) Cartridge ) P/N# SEP-. mm in-line filter for prep columns

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