SAFETY DATA SHEET 1. Identification of the substance/mixture and of the company/undertaking Identification of the substance/preparation Product name 5% PHENYLISOTHIOCYNATE IN N-HEPTANE PROTEIN SEQUENCING REAGENT Company/Undertaking Identification Life Technologies 5791 VAN ALLEN WAY PO BOX 6482 CARLSBAD, CA 92008 +1 760 603 7200 INVITROGEN CORPORATION 5250 MAINWAY DRIVE BURLINGTON, ONT CANADA L7L 6A4 800/263-6236 24 hour Emergency Response (Transport): 1-800-424-9300 Outside of the U.S. +1-703-527-3887 For research use only Not intended for human or animal diagnostic or therapeutic uses Page 1 / 8
2. Hazards identification GHS - Classification Signal Word DANGER GHS Physical Hazard Graphic GHS Physical Hazard UN Graphic GHS Final Health Effects Graphic Aquatic Toxicity Graphic Health Hazard Aspiration toxicity Category 1 Acute oral toxicity Category 4 Acute dermal toxicity Category 5 Skin Corrosion/Irritation Category 1 B Serious Eye Damage/Eye Irritation Category 2A Respiratory Sensitization Category 1 Skin Sensitization Category 1 Specific target organ systemic toxicity (single exposure) Category 3 Page 2 / 8
Physical Hazards GHS Physical Hazard 1 Flammable liquids GHS Physical Hazard Category Number Category 2 Hazard statements H302 - Harmful if swallowed H314 - Causes severe skin burns and eye damage H304 - May be fatal if swallowed and enters airways H317 - May cause an allergic skin reaction H334 - May cause allergy or asthma symptoms or breathing difficulties if inhaled H336 - May cause drowsiness and dizziness H410 - Very toxic to aquatic life with long lasting effects Physical Hazard Statements Highly flammable liquid and vapor Precautionary statements P210 - Keep away from heat/sparks/open flames/hot surfaces. - No smoking P261 - Avoid breathing dust/fume/gas/mist/vapors/spray P273 - Avoid release to the environment P280 - Wear protective gloves/protective clothing/eye protection/face protection P303 + P361 + P353 - IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower P304 + P340 - IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing Principle Routes of Exposure/ Potential Health effects Eyes Skin Inhalation Ingestion Specific effects carcinogenic effects mutagenic effects Reproductive toxicity Sensitization Aggravated Medical Conditions Causes burns. Irritating to eyes. Causes burns. Harmful in contact with skin. Causes sensitization. Irritating to skin. Harmful by inhalation. Causes burns. May cause sensitization by inhalation. Harmful! Can cause moderate respiratory irritation, dizziness, weakness, fatigue, nausea and headache. Can cause systemic damage. Irritating to respiratory system. Causes burns. R65 - Harmful: may cause lung damage if swallowed. Toxic if swallowed. not applicable not applicable not applicable R42 - May cause sensitization by inhalation R43 - May cause sensitization by skin contact Respiratory system Skin Central nervous system (CNS) Central Vascular System (CVS) HMIS Health 4 * Chronic Hazard Page 3 / 8
Flammability 3 Reactivity 0 3. Composition/information on ingredients Chemical Name CAS-No EINECS-No Weight % Phenyl Isothiocyanate 103-72-0 203-138-1 3-7 Heptane (n-) 142-82-5 205-563-8 60-100 We recommend handling all chemicals with caution. 4. First aid measures Skin contact Eye contact Ingestion Inhalation Notes to physician Wash off immediately with plenty of water. Remove and wash contaminated clothing before re-use. Immediate medical attention is required. Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician. Call a physician or Poison Control Centre immediately. Never give anything by mouth to an unconscious person. Do not induce vomiting without medical advice. Move to fresh air. Oxygen or artificial respiration if needed. Consult a physician. Treat symptomatically. 5. Fire-fighting measures Suitable extinguishing media Special protective equipment for firefighters Water spray. Carbon dioxide (CO2). Foam. Dry chemical. As in any fire, wear self-contained breathing apparatus pressure-demand, MSHA/NIOSH (approved or equivalent) and full protective gear. Use personal protective equipment. 6. Accidental release measures Personal precautions Methods for cleaning up Ensure adequate ventilation. Avoid contact with skin, eyes and clothing. Use personal protective equipment. Soak up with inert absorbent material (e.g. sand, silica gel, acid binder, universal binder, sawdust). Prevent product from entering drains. Environmental precautions Prevent further leakage or spillage if safe to do so See Section 12 for additional information. 7. Handling and storage Handling Storage Always wear reccommended Personal Protective Equipment. Keep container tightly closed in a dry and well-ventilated place. Keep away from open flames, hot surfaces and sources of ignition. Page 4 / 8
8. Exposure controls/personal protection Exposure limits Chemical Name Heptane (n-) OSHA PEL 2000 mg/m 3 500 ppm OSHA PEL (Ceiling) ACGIH OEL (TWA) - 400 ppm ACGIH OEL (STEL) 500 ppm Engineering measures Ensure adequate ventilation, especially in confined areas. Personal protective equipment Respiratory protection When workers are facing concentrations above the exposure limit they must use appropriate certified respirators. Hand protection Eye protection Skin and body protection Hygiene measures Environmental exposure controls Impervious gloves. Tightly fitting safety goggles. Avoid contact with eyes. impervious clothing. Skin and body protection. Avoid contact with skin, eyes and clothing. Handle in accordance with good industrial hygiene and safety practice. Do not allow material to contaminate ground water system Prevent product from entering drains 9. Physical and chemical properties General Information Form Appearance Odor Boiling Point/Range Melting point/range Flash point Autoignition temperature Oxidizing properties Water solubility liquid C no data available C no data available C no data available C no data available no data available F no data available F no data available F no data available F no data available 10. Stability and reactivity Stability Materials to avoid Hazardous decomposition products polymerization Stable under normal conditions. Strong oxidizing agents. Strong bases. Amines. Acids. Water. Alcohols. Carbon oxides. nitrogen oxides (NOx). Sulphur oxides. Acute toxicity 11. Toxicological information Page 5 / 8
Chemical Name Phenyl Isothiocyanate Heptane (n-) LD50 (oral,rat/mouse) LD50 (dermal,rat/rabbit) = 87 mg/kg (Mouse) no data available = 5000 mg/kg (Mouse) 3,000 mg/kg (rabbit) LC50 (inhalation,rat/mouse) no data available =103g/m 3 (Rat) Principle Routes of Exposure/ Potential Health effects Eyes Skin Inhalation Ingestion carcinogenic effects mutagenic effects Reproductive toxicity Sensitization Aggravated Medical Conditions Causes burns. Irritating to eyes. Causes burns. Harmful in contact with skin. Causes sensitization. Irritating to skin. Harmful by inhalation. Causes burns. May cause sensitization by inhalation. Harmful! Can cause moderate respiratory irritation, dizziness, weakness, fatigue, nausea and headache. Can cause systemic damage. Irritating to respiratory system. Causes burns. R65 - Harmful: may cause lung damage if swallowed. Toxic if swallowed. R42 - May cause sensitization by inhalation. R43 - May cause sensitization by skin contact. Respiratory system Skin Central nervous system (CNS) Central Vascular System (CVS) 12. Ecological information Ecotoxicity effects Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment Acute aquatic toxicity Category 1 Chronic aquatic toxicity Category 1 Mobility Biodegradation Bioaccumulation Chemical Name Heptane (n-) 142-82-5 Freshwater Algae Data Water Flea Data Daphnia magna EC50>10 mg/l (24 h) Freshwater Fish Species Data Microtox Data log Pow logpow4.66 13. Disposal considerations Dispose of in accordance with local regulations 14. Transport information Page 6 / 8
IATA Proper shipping name Flammable liquid, toxic, n.o.s. (Phenyl Isothiocyanate, Heptane) Hazard class 3 Subsidiary Class 6.1 Packing group II UN-No UN1992 15. Regulatory information Component Phenyl Isothiocyanate 103-72-0 ( 3-7 ) Heptane (n-) 142-82-5 ( 60-100 ) TSCA Listed Listed U.S. Federal Regulations SARA 313 This product is not regulated by SARA. Clean Air Act, Section 112 Hazardous Air Pollutants (HAPs) (see 40 CFR 61) This product does not contains HAPs. U.S. State Regulations Chemical Name Massachusetts - RTK New Jersey - RTK Pennsylvania - RTK Illinois - RTK Rhode Island - RTK Heptane (n-) Listed Listed Listed - Listed California Proposition 65 This product does not contain chemicals listed under Proposition 65 WHMIS hazard class: B2 Flammable liquid D2B Toxic materials This product has been classified according to the hazard criteria of the CPR and the MSDS contains all of the information required by the CPR 16. Other information Reason for Revision not applicable. (M)SDS sections updated. Page 7 / 8
For research use only Not intended for human or animal diagnostic or therapeutic uses The above information was acquired by diligent search and/or investigation and the recommendations are based on prudent application of professional judgment. The information shall not be taken as being all inclusive and is to be used only as a guide. All materials and mixtures may present unknown hazards and should be used with caution. Since the Company cannot control the actual methods, volumes, or conditions of use, the Company shall not be held liable for any damages or losses resulting from the handling or from contact with the product as described herein. THE INFORMATION IN THIS MSDS DOES NOT CONSTITUTE A WARRANTY, EXPRESSED OR IMPLIED, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE. End of Safety Data Sheet Page 8 / 8