Integrted Biologicl Control of Woolly Apple Aphid Apple Crop Protection Reserch Review Ykim Convention Center, Ykim, WA 27 Jnury, 211 2:45 pm Elizeth H. Beers WSU Tree Fruit Reserch & Extension Center 11 N. Western Ave. Wentchee, Wshington
3-yer project (28-21) Identify nd quntify the predtors nd prsitoids of woolly pple phid in estern Wshington (yer 1). Determine the reltive impct of the predtors nd prsitoids (yers 2 nd 3). Determine the effect of orchrd pesticides on the key nturl enemies (yers 2 nd 3).
Oj. 1: ID, quntify nturl enemies Other, 4.8% Lcewings, 23.6% *Aphelinus mli only prsitoid found Ldy Beetles, 8.9% Syrphids, 62.7% Other, 3.% Lcewings, 6.% Lcewings, 12.% 28 Ldy Beetles, 13.% Ldy Beetles, 24.% Syrphids, 62.% Syrphids, 81.% 26 27
Colonies/5-min serch 8 6 4 2 Mid-seson Oj. 1: side enefit (WAA sesonl phenology - 28) Apr My Jun Jul Aug Sep Oct Nov 12 Mid- nd Lte Seson 8 4 12 8 Lte Seson 25 2 15 Apr My Jun Jul Aug Sep Oct Nov 4 1 5 Apr My Jun Jul Aug Sep Oct Nov
Oj. 2: Reltive impct of predtors nd prsitoids Mid-summer Fll 15 15 WAA only WAA + A. mli WAA + A. mli + predtors Men WAA/tree 1 5 Men WAA/tree 1 5 21-Jun 28-Jun 5-Jul 12-Jul 19-Jul 26-Jul 2-Aug c 6-Sep 13-Sep 2-Sep 27-Sep 4-Oct 11-Oct 18-Oct 25-Oct c
Entrust Lorsn Sevin Guthion Clypso Assil Wrrior II Cyzpyr Oj. 3: Impct of Pesticides on A. mli 31. Acute toxicity Kumulus Ultor Koc+Mn.1x 1x 2 4 6 8 1 Corrected % Mortlity
3.5 Fecundity.7 Proportion Femles 3..6 Men Dily Fecundity % Mortlity 2.5 2. 1.5 1..5. 3 25 2 15 1 Mortlity Control Proportion Femles R (Popultion Growth).5.4.3.2.1. 1.8 1.6 1.4 1.2 1..8.6 R Control A. mli Sulethl Biossys 32. Sulethl effects 5.4.2 Control. Control
Oj. 3: Impct of Pesticides on A. mli 32. Sulethl effects Prsitoid Aundnce (Adults) 1..8.6.4.2 13 Control. 2 4 6 8 1 Time (dys)
Oj. 3. Pesticide effects Lrge plots Trt. 1 2 3 4 PF (1 DD) 32 fl oz Intrepid 16 fl oz 32 fl oz Intrepid 16 fl oz 1st cover (35 DD) 7 oz 7 oz 4.5 oz 4.5 oz 2nd cover (+17 d) 7 oz 7 oz 4.5 oz 4.5 oz 4 trts x 4 replictes Ech rep 1-1.5 cres (Totl 16-2 cres) Smpled every other week April-Nov: WAA, GAA, mites WAA predtors/pr HIPV trps A. mli sticky crds Erwig trps Tp Counts
29 Lrge Plot: Bridgeport 5 91 WAA colonies/5 min 4 3 2 1 - Intrepid- - Intrepid- Intrepid- Woolly Apple Aphid d - My Jun Jul Aug Sep Oct Nov Dec Intrepid- c - 2 4 6 8 1 12 WAA colonies (sesonl men)
29 Lrge Plot: Bridgeport 25 2 Erwigs Erwigs/trp 15 1 5 Intrepid- - Jun Jul Aug Sep Oct Nov 91 - Intrepid- - Intrepid- Intrepid- - 2 4 6 8 1 12 14 Erwigs/trp (sesonl sum)
29 Lrge Plot: Othello 25 911 WAA colonies/5 min 2 15 1 5 - Intrepid- - Intrepid- Intrepid- Woolly Apple Aphid c - c My Jun Jul Aug Sep Oct Nov Dec Intrepid- - 1 2 3 4 5 6 7 WAA colonies (sesonl men)
21 Lrge Plot: Othello 4 111 WAA colonies/5 min 3 2 1 - Intrepid- - Intrepid- Apr My Jun Jul Aug Sep Oct Nov Dec Intrepid- - Intrepid- Woolly Apple Aphid 29 c 21 c c - 1 2 3 4 5 6 7 WAA colonies (sesonl men)
Trt. 1 2 3 4 Oj. 3. Pesticide effects Lrge plots protocol #2 PF (1 DD) Intrepid 16 fl oz Intrepid 16 fl oz Intrepid 16 fl oz Intrepid 16 fl oz 1st cover (35 DD) 7 oz 4.5 oz Wrrior 2.56 fl oz Guthion 2 l 2nd cover (+17 d) 7 oz 4.5 oz Wrrior 2.56 fl oz Guthion 2 l 4 trts x 4 replictes Ech rep 1 cres (Totl 16 cres) Smpled every other week April-Nov: WAA, GAA, mites WAA predtors/pr HIPV trps A. mli sticky crds Erwig trps Tp Counts
21 Lrge Plot: Othello #2 12 113 WAA colonies/5 min 1 8 6 4 2 Wrrior II Guthion Guthion Woolly Apple Aphid Wrrior II c Apr My Jun Jul Aug Sep Oct Nov Dec 5 1 15 2 25 3 WAA colonies (sesonl men)