Aeris Precision Engineered Core- Shell Particles for Ultra-High Resolution BioSeparations Aeris is a specialized line of reversed phase core-shell UHPLC columns, built exclusively for the ultra-high performance separation and analysis of proteins and peptides. Core-shell particle technology provides striking increases in peak capacity and resolution at lower backpressures, giving chromatographers the ability to use longer (or coupled) columns for BioSeparations. Additionally, optimizing the pore size and shell thickness for intact proteins or smaller peptide fragments leads to maximum separation power. If Aeris core-shell columns do not provide at least an equivalent separation as compared to a competing column of the same phase, return the column with the comparative data within 45 days for a FULL REFUND. For more in-depth information about Aeris core-shell columns and to select the right column for your application, please visit p. 295 Aeris PEPTIDE Columns for Peptide and Peptide Mapping Separations Aeris PEPTIDE particles are designed with small pores, inert XB- C18 surface chemistry, and three different particle sizes (3.6 µm, 2.6 µm, and 1.7 µm) to meet the resolution demands of chromatographers performing complex peptide and peptide map separations on HPLC and/or UHPLC systems. Recommended for: Synthetic peptide impurity analysis Peptide mapping Identifying protein modifications Glycosylation, Substitution, and Truncation Analyzing post-translational modifications Deamidation, Oxidation, and Deletions 3.6 µm Aeris PEPTIDE 2.6 µm Small pore optimized for peptides and for peptide mapping 1.7 µm 3.6---3.2 solid core 3.6---2.6 solid core 1.7---1.25 solid core AERIS UHPLC 3.6---3.2 solid core 3.6---2.6 solid core 1.7---1.25 solid core Aeris WIDEPORE Columns for Intact Protein and Polypeptide Separations Aeris WIDEPORE columns are packed with 3.6 µm core-shell particles that are specially engineered with a thin porous shell, large pores, and sterically protected XB surface chemistry resulting in low backpressures, fast rates of diffusion, and excellent selectivity, generating exceptional chromatographic resolution on UHPLC systems. Recommended for: Protein structural characterization Stability indicating assays Post-translational modification identification PEGylated proteins, antibodies, biogenerics, etc. Impurity profiling Peptide mapping Aeris WIDEPORE 3.6 µm Large pore optimized for intact proteins and polypeptides 286 Phenomenex
Aeris Maximize UHPLC Resolving Power with Unique 3.6 µm Core-Shell Particle 3.6 µm core-shell technology combined with inert surface chemistries and tight packing specifications results in Aeris WIDEPORE columns delivering exceptional resolving power at significantly lower backpressures. Chromatographers now have the ability to generate higher quality data than typically produced by columns packed with fully porous particles for every protein analysis. Performance Equivalent to Sub-2 µm Particle at Low Backpressure Waters ACQUITY BEH3 1.7 µm C18 Aeris WIDEPORE 3.6 µm XB-C18 UHPLC Results at 1 /3 the Backpressure 8 8 6 42 bar 6 14 bar 4 2 App ID 19912 4 2 App ID 19838 16 18 2 22 24 26 28 min 16 18 2 22 24 26 28 min Conditions for both columns: Column: ACQUITY BEH3 1.7 µm C18 Aeris WIDEPORE 3.6 µm XB-C18 Dimensions: 15 x 2.1 mm Mobile Phase: A: Water with.1 % TFA B: Acetonitrile with.1 % TFA Gradient: A/B (97:3) to A/B (35:65) over 45 min Flow Rate:.3 ml/min Temperature: 4 ºC Injection Volume: 1 µl Instrument: Agilent 12 Detection: UV @ 214 nm (ambient) Sample: Human Epidermal Growth Factor (EGF) Increase Efficiency on UHPLC Systems with Sub-2 µm Core-Shell Particles Aeris PEPTIDE 1.7 µm core-shell columns are an excellent solution for ultra-high resolution peptide and peptide mapping separations. Core-shell particle technology combined with a sub-2 µm particle size results in extremely high efficiencies that scientists can use to pull apart critical peaks. Ultra-High Resolution Achieved with 1.7 µm Core-Shell Technology AU.1 Aeris PEPTIDE 3.6 µm XB-C18 3.6 µm App ID 19894 AU.3.2.1 Increased Resolution and Sensitivity Aeris PEPTIDE 1.7 µm XB-C18 1.7 µm App ID 19897 UHPLC AERIS 2 4 6 8 1 12 min 2 4 6 8 1 12 min Conditions for both columns: Column: Aeris PEPTIDE 3.6 µm XB-C18 Aeris PEPTIDE 1.7 µm XB-C18 Dimensions: 15 x 2.1 mm Part Nos.: F-457-AN F-456-AN Mobile Phase: A: Water with.1 % TFA B: Acetonitrile with.8 % TFA Gradient: A/B (97:3) for 1.5 min to A/B (6:4) over 11 min to A/B (5:95) for 1 min Flow Rate:.5 ml/min Temperature: 4 ºC Injection Volume: 5 µl Instrument: Agilent 12SL Detection: UV @ 214 nm (ambient) Sample: Alpha-Casein Tryptic Digest Waters and ACQUITY are registered trademarks, and BEH Technology is a trademark of Waters Corporation. Phenomenex is not affiliated with Waters Corporation. Study was performed using new columns and, to the extent possible, identical experimental conditions were applied. Comparative separations may not be representative of all applications. Phenomenex 287
Aeris Greater Method Flexibility with Specialty Surface Chemistries Aeris WIDEPORE columns are available in three surface chemistries (XB-C18, XB-C8, C4) to satisfy applications of all types, ranging from sticky, intact proteins to complex protein digests. Aeris PEPTIDE columns utilize the XB-C18 chemistry, as it is excellent for peptides and peptide mapping applications. The unique, sterically protected XB surface ligands are designed by bonding bulky isobutyl chains aside the alkyl chains, and then fully endcapping the surface to cover any remaining exposed silanols. Material Characteristics Total Particle Size (µm) Porous Shell (µm) If Aeris core-shell columns do not provide at least an equivalent separation as compared to a competing column of the same phase, return the column with the comparative data within 45 days for a FULL REFUND. Core Size (µm) ph Stability Temp Stability Pressure Stability Packing Material Aeris WIDEPORE 3.6.2 3.2 1.5-9 9 C 6 bar Aeris PEPTIDE 1.7.22 1.25 1.5-9 9 C 1 bar Aeris PEPTIDE 2.6.35 1.9 1.5-9 9 C 1 bar Aeris PEPTIDE 3.6.5 2.6 1.5-9 9 C 6 bar Aeris PEPTIDE 1.7 µm Minibore Columns (mm) Phases 5 x 2.1 1 x 2.1 15 x 2.1 3/pk Protective isobutyl side chains XB-C18 B-456-AN D-456-AN F-456-AN AJ-8948 Aeris PEPTIDE 2.6 µm Minibore Columns (mm) Phases 15 x 2.1 25 x 2.1 3/pk Si Si XB-C18 F-455-AN G-455-AN AJ-8948 Aeris PEPTIDE 2.6 µm Analytical Columns (mm) Phases 15 x 4.6 25 x 4.6 3/pk XB-C18 F-455-E G-455-E AJ-8946 for 4.6 mm ID Aeris PEPTIDE 3.6 µm Minibore Columns (mm) Phases 5 x 2.1 1 x 2.1 15 x 2.1 25 x 2.1 3/pk XB- C18 C4 XB- C8 AERIS UHPLC XB-C18 B-457-AN D-457-AN F-457-AN G-457-AN AJ-8948 Aeris PEPTIDE 3.6 µm Analytical Columns (mm) Phases 5 x 4.6 1 x 4.6 15 x 4.6 25 x 4.6 3/pk XB-C18 B-457-E D-457-E F-457-E G-457-E AJ-8946 for 4.6 mm ID Aeris WIDEPORE 3.6 µm Minibore Columns (mm) Phases 5 x 2.1 1 x 2.1 15 x 2.1 25 x 2.1 3/pk XB-C18 B-4482-AN D-4482-AN F-4482-AN G-4482-AN AJ-8783 XB-C8 B-4481-AN D-4481-AN F-4481-AN G-4481-AN AJ-8785 C4 B-4486-AN D-4486-AN F-4486-AN G-4486-AN AJ-8899 Aeris WIDEPORE 3.6 µm Analytical Columns (mm) Phases 5 x 4.6 1 x 4.6 15 x 4.6 25 x 4.6 3/pk ULTRA Holder with cartridge Cartridge Holder XB-C18 B-4482-E D-4482-E F-4482-E G-4482-E AJ-8769 XB-C8 B-4481-E D-4481-E F-4481-E G-4481-E AJ-8771 C4 B-4486-E D-4486-E F-4486-E G-4486-E AJ-891 * ULTRA cartridges require holder part number, AJ-9 for 4.6 mm ID ULTRA Cartridge Holder* (for 2.1 to 4.6 mm ID columns) ULTRA Guard Cartridge Holder ea Price AJ-9 For more about ULTRA, see p. 255. For Core-Shell Performance Enhancement Kit, see p. 369 288 Phenomenex
Clarity Core-Shell Columns for Synthetic DNA/RNA Clarity Oligo-MS UHPLC Columns Rapid and Efficient LC/MS Separation for QC and Characterization Core-shell particle technology provides improved speed, resolution, and sensitivity Clarity Oligo-MS, C18 columns have been engineered for the MS characterization of synthetic DNA and RNA samples. This media is based on core-shell technology which generates extremely high efficiencies due to the innovative particle design. This increase in efficiency improves the resolution between critical oligo sequences, gives higher sensitivity for easer MS quantitation, and allows for a decrease in column length for higher throughput. Core-Shell Technology for Synthetic DNA/RNA Analysis Clarity Oligo-MS media is not fully porous like traditional particles used for the analysis of oligonucleotides. It is a core-shell particle technology which uses a sol-gel processing technique to grow a homogeneous porous shell onto a solid core. This highly optimized process combined with uniform particle size distribution produces a column that generates extremely high plate counts. Achieve Baseline Resolution of N-1 and N+1 Oligo from Target The high plate counts generated by the Clarity Oligo-MS material produce extremely high efficiencies and thus excellent resolution between oligonucleotides of similar length and structure. Scientists can achieve baseline resolution between synthetic oligonucleotides with just one base difference allowing easier quantitation. Poly dt Standard (12-18mer) Column: Clarity 2.6 µm Oligo-MS C18 Dimensions: 5 x 2. mm Part No.: B-4479-BO Mobile Phase: A: 1 mm HFIP / 4 mm TEA / 2 % Methanol B: 1 mm HFIP / 4 mm TEA / 98 % Methanol Gradient: A/B (95:5) to A/B (8:2) in 1 min Flow Rate:.5 ml/min Temperature: 5 C Detection: UV @ 26 nm (22 C) Injection Volume: 2 µl Sample: Poly dt (12-18) If analytical Clarity LC products do not provide at least an equivalent separation as compared to a competing product of the same particle size, similar phase and dimensions, return the column with comparative data within 45 days for a FULL REFUND. 1.7 µm Core-Shell Particle Boost performance of existing sub-2 µm methods 1.25 µm Solid Core 2.6 µm Core-Shell Particle.23 µm Porous Shell 1.7 µm Deliver increased efficiency at reduced backpressures for UHPLC performance on any system 1.9 µm Solid Core Clarity Oligo-MS HPLC Columns Minibore Columns (mm) Phases 5 x 2.1 1 x 2.1 1.7 µm Oligo-MS C18 B-448-AN D-448-AN.35 µm Porous Shell 2.6 µm 8 6 4 2 2 4 6 8 1 12 min Rapid Separation of Complex Oligo Samples Due to the high resolving power of Clarity Oligo-MS columns, high-throughput methods for the separation of complex synthetic mixtures can be developed. Using short (5 mm length) columns, impurities are separated from the peak of interest in less than 12 minutes. Crude DNA 3mer Column: Clarity 2.6 µm Oligo-MS C18 Dimensions: 5 x 2. mm Part No.: B-4479-BO Mobile Phase: A: 1 mm HFIP / 4 mm TEA / 2 % Methanol B: 1 mm HFIP / 4 mm TEA / 98 % Methanol Gradient: A/B (95:5) to A/B (8:2) in 1 min Flow Rate:.5 ml/min Temperature: 5 C Detection: UV @ 26 nm (22 C) Injection Volume: 2 µl Sample: Crude DNA 3mer App ID 18836 UHPLC CLARITY OLIGO-MS 2.6 µm Oligo-MS C18 B-4479-AN D-4479-AN 6 Analytical Columns (mm) Phase 5 x 4.6 1 x 4.6 2.6 µm Oligo-MS C18 B-4479-E D-4479-E * Column ID (mm) Material Description ph Stability 2.1 4.6 /3pk /3pk Oligo-MS C18 (ODS, Octadecyl) 1.5 8.5* AJ-968 AJ-966 *ph stable 1.5 8.5 under gradient conditions. ph stable 1.5 1 under isocratic conditions. ** ULTRA cartridges require holder, Part No.: AJ-9. Phenomenex 4 2 2 4 6 8 1 12 min For more about ULTRA and cartridge holder ordering information, see p. 255. For Core-Shell Performance Enhancement Kit, see p. 369 App ID 18837 289
ULTRA UHPLC Column Protection System for Any Sub-2 μm Column Increases column lifetime of virtually all manufacturers UHPLC, sub-2 μm, and core-shell columns Offers more reproducible chromatography For pressures up to 2 psi (1378 bar) ULTRA Increases Column Lifetime, Guaranteed! When contaminates and particulates build up at the head of your column or on the guard cartridge, system backpressures can increase dramatically. By simply replacing the ULTRA cartridge, instead of your column, you are able to regain normal operating conditions and reclaim original column performance. Accelerated lifetime test using endogenous biological matrix on Kinetex 2.6 µm C18 5 x 4.6 mm ID column UHPLC Column Protection 212 R&D 1 Award Recipient Frit Material Chamber If ULTRA cartridge protection system does not perform as well or better than your current guard cartridge system of similar phase and dimensions, return the product with the comparative data within 45 days for a FULL REFUND See it in action: www.phenomenex.com/ultra Cartridge* Holder SECURITYGUARD ULTRA UHPLC 8 6 4 2 Backpressure (Bar) 3 28 26 24 22 2 18 16 14 12 1 1 ULTRA Performance Cartridge Replacement Protects with No Loss of Column Performance! With ULTRA, contaminants and microparticulates are trapped within the guard cartridge instead of in your expensive UHPLC column. The extremely low dead volume (<.3 μl) of this unique guard design minimizes sample peak dispersion to maintain column performance without altering your chromatography results. With SG ULTRA No Guard Injection No. WITHOUT ULTRA WITH ULTRA 2 Cartridge Replacement With ULTRA..2.4.6.8 1. 1.2 1.4 min Conditions for both columns: Column: Kinetex 1.7 µm XB-C18 Sample: 1. Uracil Dimensions: 5 x 2.1 mm 2. Acetophenone Guard Cartridge: ULTRA C18 (ODS) 2.1 mm ID 3. Toluene Part No.: AJ-8768 4. Naphthalene Mobile Phase: Acetonitrile / Water (5:5) Flow Rate:.5 ml/min Detection: UV @ 254 nm Without SG ULTRA Parameters Difference Selectivity 1.36 1.35 -.99 % Efficiency (Plates/Meter) 237,22 246,8-3.6 % Backpressure 36 348 3.45 % 3 4 App ID 19859 App ID 1986 Compounds of Interest } Contaminants and Particulates *Cartridge schematic not drawn to scale Holder with cartridge ULTRA Cartridge Holder Part No. Description Unit Price AJ-9 ULTRA Cartridge Holder ea ULTRA Cartridges Cartridges for General Purpose/ Pharmaceutical Column ID (mm) Material Description ph Stability 2.1 3. 4.6 /3pk /3pk /3pk C18 (ODS, Octadecyl) 1.5 8.5* AJ-8782 AJ-8775 AJ-8768 C8 (MOS, Octyl) 1.5 8.5* AJ-8784 AJ-8777 AJ-877 PFP (Pentafluorophenyl) 1.5 8.5* AJ-8787 AJ-878 AJ-8773 HILIC HILIC 2. 7.5 AJ-8786 AJ-8779 AJ-8772 Phenyl (Phenylhexyl) 1.5 8.5* AJ-8788 AJ-8781 AJ-8774 Cartridges for Protein and Peptide Reversed Phase Widepore C18 (ODS, Octadecyl) 1.5 8.5* AJ-8783 AJ-8769 Widepore C8 (MOS, Octyl) 1.5 8.5* AJ-8785 AJ-8771 Widepore C4 (Butyl) 1.5 8.5* AJ-8899 AJ-891 Peptide C18 (ODS, Octadecyl) 1.5 8.5* AJ-8948 AJ-8946 Cartridges for Synthetic DNA / RNA Analysis Oligo-MS C18 (ODS, Octadecyl) 1.5 8.5* AJ-968 AJ-967 AJ-966 *ph stable 1.5 8.5 under gradient conditions. ph stable 1.5 1 under isocratic conditions. Initial ULTRA installation onto Kinetex, Aeris, and Oligo-MS columns and later cartridge replacement, requires 3 wrenches, which must be purchased separately: one 3/8 in. wrench (AQ-8959; fits Kinetex, Aeris, and Oligo-MS column end-fittings), and two 5/16 in. wrenches (AQ-893; fits ULTRA cartridge and holder). See p. 376 29 Phenomenex
BioSeparations BIOSEPARATIONS 291-324 Amino Acid Analysis EZ:faast... 292 Proteins/Peptides Aeris... 295 BioSep... 34 Jupiter... 36 Yarra... 39 Yarra PREP... 314 Synthetic DNA/RNA Clarity... 315