Laboratory Accreditation Programmes

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Client No. 1609 LABNET Invermay Limited PO Box 371, Mosgiel, 9053 Puddle Alley, RD 2, Mosgiel, 9092 Telephone 03 489-4600 www.gribblesvets.co.nz Fax 03 489-8576 Authorised Representative Ms Denise Carian-Smith Quality and Technical Manager Programme Biological Testing Laboratory Accreditation Number 391 Initial Accreditation Date 7 September 1989 Conformance Standard NZS ISO/IEC 17025:2005 General requirements for the competence of testing and calibration laboratories Testing Services Summary General 1.11 Foods 1.12 Waters 1.71 Biological Condition Key Technical Personnel Ms Denise Carian-Smith Mr Brent Hananeia Mr Matthew McDonald Ms Denise Carian-Smith 1.02 (a) 1.02 (a) 1.02 (a) 1.02 (b) Issue 38 Date: 27/06/18 Page 1 of 7

Ms Victoria Fitiao 1.02 (f) General Ms Victoria Fitiao 1.11, 1.12, 1.71 Miss Antoinette McIntosh 1.11, 1.12, 1.71 Issue 38 Date: 27/06/18 Page 2 of 7

(a) The following tests in accordance with the Roche/Hitachi Modular Manufacturer s method: Alanine Aminotransferase (ALT) Albumin Alkaline Phosphatase Amylase Aspartate Aminotransferase (AST) Bicarbonate Bilirubin Calcium Chloride Cholesterol Creatine Kinase (CK) Creatinine Gamma Glutamyl Transferase (GGT) Glucose Glutamate dehydrogenase (GLDH) Lipase Magnesium Phosphate Potassium Sodium Total protein Urea The following tests in accordance with the methods indicated: β-hydroxybutyrate Clinical Chem. (1984) 30.3 Copper AOAC (On-line Edition) 968.08 & 983.24 Ferroxidase Tietz (1976) Fundamentals of Clinical Chemistry 2 nd Edition Glutathione peroxidise RES-Vet-Sci (1980) 28, 321-324 Selenium JAOAC 1988 (vol. 7) Vitamin B12 British Journal of (22:507) 1974 Zinc AOAC (On-line Edition) 968.08 & 991.11 Lead (blood) Perkin Elmer Notice 2011 Issue 38 Date: 27/06/18 Page 3 of 7

(b) Routine analysis of canine, feline, ovine, cervine, equine, bovine, caprine, porcine and zoo/exotic blood and plasma for: Complete blood count using Celldyne 3700 cell counter: Haematocrit (HCT) Haemoglobin Mean Cell Haemoglobin (MCH) Mean Cell Haemoglobin Concentration (MCHC) Mean Cell Volume (MCV) Platelets Red cell count White cell count The following haematology tests by the methods indicated: Blood film staining Heinz bodies Packed cell volume Parasite identification Reticulocyte count Leishman s stain Methylene blue method Microhaematocrit method Microscopy Methylene blue method Differential cell count using methods as indicated: Platelet morphology Red cell morphology White cell differential White cell morphology Issue 38 Date: 27/06/18 Page 4 of 7

(f) Conducting the following staining technique: Bacterial Gram Cultures for the following enteric pathogens: Campylobacter Yersinia Antibiotic sensitivity testing (Kirby-Bauer method) Routine aerobic and anaerobic cultures; unknown pathogens with identification of organisms by biochemical testing General 1.11 Foods (a) (c) (d) (e) (g) (h) (i) Cereals and cereal products Nuts, fruits, vegetables and derived products Sauces, herbs, spices and condiments Sugar and sugar confectionery Meat, poultry and derived products Fish and fish products Eggs and egg products APHA Compendium of Methods for the Microbiological Examination of Foods (5 th Edition) unless where otherwise stated Aerobic plate count APHA 8 Bacillus cereus APHA 31 Campylobacter FDA BAM Chapter 7 Clostridium perfringens APHA 33 Coagulase positive Staphylococcus APHA 39 Coliform - MPN APHA 9 E.coli MPN APHA 9 E.coli / Coliforms (Petrifilm) APHA 9.935 Enterobacteriaceae APHA 9 Issue 38 Date: 27/06/18 Page 5 of 7

Faecal Coliforms - MPN APHA 9 Listeria 3M MDS Listeria V2 (AOAC 2016.07) 3M MDS MIMM 7.7 (g) only (culture method) FDA BAM Chapter 5 Shigella APHA 37 Yeasts and moulds APHA 21 (h) Fish and fish products FDA BAM Chapter 5 1.12 Waters (a) Potable waters E.coli MIMM 11.A2 Faecal Coliforms MIMM 11.A2 Total Aerobic Plate Count MIMM 11.6.2 Total Coliforms MIMM 11.A2 1.71 Biological Condition (a) Air Aerobic plate count APHA 3 Yeasts and mould APHA 3 (b) Plant hygiene evaluation Coliforms In-house method Enterobacteriaceae APHA 9 Listeria 3M MDS Listeria 3M MDS Listeria V2 (AOAC 2016.07) FDA BAM Ch 5 3M MDS Total count In-house method (FMI-TPM-029) References: APHA Comp FDA BAM MIMM APHA Compendium of Methods for the Microbiological Examination of Foods (5 th Edition) United States Food and Drug Administration Bacteriological Analytical Manual (8 th Edition) and Online Meat Industry Microbiological Methods (5 th Edition) Issue 38 Date: 27/06/18 Page 6 of 7

Issue 38 Date: 27/06/18 Page 7 of 7